P29435 (GYRB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B
EC=5.99.1.3

Gene names

Name:	gyrB
Ordered Locus Names:	BUsg_010

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	803 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 803	803	DNA gyrase subunit B HAMAP MF_01898		PRO_0000145299
Experimental info
	Sequence conflict	146	1	K → E in AAC38108. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29435 [UniParc]. Last modified August 30, 2002. Version 3. Checksum: F4E2BDD97EA93A71	FASTA	803	92,678
	10 20 30 40 50 60 MIDTYDSSKI KILRGLDAVR KRPGMYIGDT DDGSGLHHMV FEIVDNSIDE ALAGFCKEIK 70 80 90 100 110 120 VVIHSDNSVS IKDDGRGIPT DIHPEEKISA AEVIMTVLHS GGKFDNASYK ISGGLHGVGI 130 140 150 160 170 180 SVVNALSEKL ELIIYKNKKK YRQQYKNGNP ESPLCIIGAT EITGTYIRFW PSYKIFTNKT 190 200 210 220 230 240 QFQYEILSKR LRELSFLNSN ILIHLKDNRT NLEEKYHYKG GIKAFIKFLN KNKFPINLNI 250 260 270 280 290 300 FYFRCIKNQI ELEIAIQWNN SHQENILCFT NNIPQKDGGT HLSGFRAGIT RTLNLHIERE 310 320 330 340 350 360 GYNKKNKTII TGEDTREGLT AIISIKIPDP KFSSQTKDKL VSSEVKSVIE SLINENLIEY 370 380 390 400 410 420 LLENPIDAKF IIQKIINSAK IREAARRARE INKKKGTLDL GTLPGKLSDC QENDPKLSEI 430 440 450 460 470 480 YLVEGDSAGG SAKQGRNRKN QAILPLKGKI LNVQKTKFDK IILSQELTSL ITALGCSITK 490 500 510 520 530 540 SEYSLDKLRY NHIIIMTDAD VDGAHIRTLL LTFFYRQLPE LIKKGYVYIA QPPLYKVKKG 550 560 570 580 590 600 KQEKYIKNDE EMNKYQIKIA LKEIVIKNKK NENCNKIIKE FQKIVSDFNH IQIKMKKNKN 610 620 630 640 650 660 YFPELILNEL IYHPRLYNLK NENVVQNWIE KLVEKLNKKD KNNTIYTSKI KRNENDSIFE 670 680 690 700 710 720 PSIKLSRYAN HTQYDLKNDF LESKEYFLIT NLGEKFKKFQ ENENFIEKGE KIYKINDIKN 730 740 750 760 770 780 TLEWLIKETK RGFFVQRYKG LGEMNPDQLW NTTMNPETRN MLQVTIKDAI SANNLFNTLM 790 800 GDLVEPRRKF IQKNALKAEN IDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
[3]	"Genetic analysis of an aphid endosymbiont DNA fragment homologous to the rnpA-rpmH-dnaA-dnaN-gyrB region of eubacteria." Lai C.-Y., Baumann P. Gene 113:175-181(1992) [PubMed: 1572539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-91.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008210 Genomic DNA. Translation: AAC38108.1. AE013218 Genomic DNA. Translation: AAM67582.1. M80817 Genomic DNA. Translation: AAA73151.1.
RefSeq	NP_660371.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P29435.
SMR	P29435. Positions 10-377.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000326; EBBUCP00000000326; EBBUCG00000000326.
GeneID	1005827.
GenomeReviews	Gene locus BUsg_010 in contig AE013218_GR.
KEGG	bas:BUsg010.
PATRIC	21246775. VBIBucAph100086_0010.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008102.
HOGENOM	HBG304889.
OMA	DCSSRDP.
ProtClustDB	PRK14939.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG010-MONOMER.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 2 hits.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_BUCAP

Accession

Primary (citable) accession number: P29435

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	August 30, 2002
Last modified:	January 25, 2012
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program