P29434 (DNAA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	BUsg_012

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_0000114149
Regions
	Nucleotide binding	159 – 166	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P29434 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: A989508297256C3C	FASTA	454	52,527
	10 20 30 40 50 60 MSLCLWKQCL DRLQSELPST EFSMWIRSLK AKLNNNILEI YAPNQFILDW VKDKYLIHFK 70 80 90 100 110 120 KILQDFCGSN SPFIKFKVYQ TSKEKKFKKN ILQKIQNLNA KPIWDKIPIF KKSSHRSNIN 130 140 150 160 170 180 KKHSFENFIE GKSNQLARAA ASQVAKNPGN SYNPLFLYGG TGLGKTHLLH AIGNGILAYK 190 200 210 220 230 240 YNVKIIYMHS ERFVQDMVKA LQNNAIEKFK LYYRSVDALL IDDIQFFAHK ERSQEEFFHT 250 260 270 280 290 300 FNALLEGNQQ IILTSDRYPK EINGVEDRLK SRFGWGLTVA IDPPELETRV AILIKKADEN 310 320 330 340 350 360 NIVLSDEVAF FIAKHLRSNV RELEGALNRV IVNANFTHRS ITVEFVREAL RDILALQEKL 370 380 390 400 410 420 VTIANIQKTV AEYYKIKVAD LLSRRRSRSV ARPRQMAMAM AKELTNHSLP EIGDAFSGRD 430 440 450 HTTVLHACRK IEQLRKENHD IKEDFSNLIR TLSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of an aphid endosymbiont DNA fragment homologous to the rnpA-rpmH-dnaA-dnaN-gyrB region of eubacteria." Lai C.-Y., Baumann P. Gene 113:175-181(1992) [PubMed: 1572539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80817 Genomic DNA. Translation: AAA73149.1. Sequence problems. AF008210 Genomic DNA. Translation: AAC38106.1. AE013218 Genomic DNA. Translation: AAM67584.1.
PIR	IQJVBA. JC1158.
RefSeq	NP_660373.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P29434.
SMR	P29434. Positions 2-79, 361-453.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000118; EBBUCP00000000118; EBBUCG00000000118.
GeneID	1005828.
GenomeReviews	Gene locus BUsg_012 in contig AE013218_GR.
KEGG	bas:BUsg012.
PATRIC	21246779. VBIBucAph100086_0012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008033.
HOGENOM	HBG695666.
OMA	DEVAFLI.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG012-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_BUCAP

Accession

Primary (citable) accession number: P29434

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program