Reviewed, UniProtKB/Swiss-Prot P29422 (CATA_MICLU)

Names and origin

Protein names

Recommended name:
    Catalase
    EC=1.11.1.6

Gene names

Name:

katA

Organism

Micrococcus luteus (Micrococcus lysodeikticus)

Taxonomic identifier

1270 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Micrococcus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
Catalytic activity	2 H2O2 = O2 + 2 H2O.
Cofactor	Heme group. NADPH.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the catalase family.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase Peroxidase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 503

503

Catalase

PRO_0000084990

Sites

Active site

61

1

Active site

133

1

Metal binding

343

1

Iron (heme axial ligand)

Secondary structure

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503

Helix Strand Turn

Details...

Beta strand

18 – 20

3

Beta strand

22 – 25

4

Turn

31 – 33

3

Helix

41 – 50

10

Beta strand

63 – 73

11

Turn

78 – 80

3

Helix

84 – 86

3

Beta strand

91 – 99

9

Beta strand

101 – 103

3

Beta strand

116 – 123

8

Beta strand

126 – 136

11

Helix

143 – 145

3

Helix

146 – 153

8

Turn

157 – 159

3

Helix

164 – 172

9

Helix

175 – 177

3

Helix

178 – 185

8

Helix

187 – 189

3

Beta strand

190 – 192

3

Helix

194 – 196

3

Beta strand

205 – 208

4

Beta strand

214 – 223

10

Helix

232 – 241

10

Helix

245 – 255

11

Beta strand

261 – 270

10

Helix

273 – 276

4

Turn

290 – 292

3

Beta strand

296 – 305

10

Helix

310 – 313

4

Turn

314 – 316

3

Helix

334 – 350

17

Helix

355 – 357

3

Helix

359 – 361

3

Beta strand

386 – 389

4

Beta strand

391 – 393

3

Beta strand

408 – 415

8

Helix

428 – 436

9

Helix

440 – 454

15

Helix

459 – 472

14

Helix

474 – 485

12

Sequences

	Sequence		Length	Mass (Da)
	P29422-1 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 8C60ADEFC0E46A09	FASTA	503	56,906
	10 20 30 40 50 60 MEHQKTTPHA TGSTRQNGAP AVSDRQSLTV GSEGPIVLHD THLLETHQHF NRMNIPERRP 70 80 90 100 110 120 HAKGSGAFGE FEVTEDVSKY TKALVFQPGT KTETLLRFST VAGELGSPDT WRDVRGFALR 130 140 150 160 170 180 FYTEEGNYDL VGNNTPIFFL RDPMKFTHFI RSQKRLPDSG LRDATMQWDF WTNNPESAHQ 190 200 210 220 230 240 VTYLMGPRGL PRTWREMNGY GSHTYLWVNA QGEKHWVKYH FISQQGVHNL SNDEATKIAG 250 260 270 280 290 300 ENADFHRQDL FESIAKGDHP KWDLYIQAIP YEEGKTYRFN PFDLTKTISQ KDYPRIKVGT 310 320 330 340 350 360 LTLNRNPENH FAQIESAAFS PSNTVPGIGL SPDRMLLGRA FAYHDAQLYR VGAHVNQLPV 370 380 390 400 410 420 NRPKNAVHNY AFEGQMWYDH TGDRSTYVPN SNGDSWSDET GPVDDGWEAD GTLTREAQAL 430 440 450 460 470 480 RADDDDFGQA GTLVREVFSD QERDDFVETV AGALKGVRQD VQARAFEYWK NVDATIGQRI 490 500 EDEVKRHEGD GIPGVEAGGE ARM

References

[1]	"Three-dimensional structure of catalase from Micrococcus lysodeikticus at 1.5-A resolution." Murshudov G.N., Melik-Adamyan W.R., Grebenko A.I., Barynin V.V., Vagin A.A., Vainshtein B.K., Dauter Z., Wilson K.S. FEBS Lett. 312:127-131(1992) [PubMed: 1426241] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
[2]	"The structures of Micrococcus lysodeikticus catalase, its ferryl intermediate (compound II) and NADPH complex." Murshudov G.N., Grebenko A.I., Brannigan J.A., Antson A.A., Barynin V.V., Dodson G.G., Dauter Z., Wilson K.S., Melik-Adamyan W.R. Acta Crystallogr. D 58:1972-1982(2002) [PubMed: 12454454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (0.88 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ438208 Genomic DNA. Translation: CAD27348.1.

PIR

S27264.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GWE	X-ray	0.88	A	1-502	[»]
1GWF	X-ray	1.96	A	1-502	[»]
1GWH	X-ray	1.74	A	1-502	[»]
1HBZ	X-ray	1.50	A	6-502	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.11.1.6. 873.

Family and domain databases

InterPro

IPR002226. Catalase.
IPR010582. Catalase-rel_immune_responsive.
IPR011614. Catalase_N.
IPR018028. Catalase_rel_subgroup.
[Graphical view]

Gene3D

G3DSA:2.40.180.10. Catalase_N. 1 hit.

PANTHER

PTHR11465. Catalase. 1 hit.

Pfam

PF00199. Catalase. 1 hit.
PF06628. Catalase-rel. 1 hit.
[Graphical view]

PRINTS

PR00067. CATALASE.

ProDom

PD000510. Catalase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00437. CATALASE_1. 1 hit.
PS00438. CATALASE_2. 1 hit.
PS51402. CATALASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CATA_MICLU

Accession

Primary (citable) accession number: P29422
Secondary accession number(s): Q8RK91

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	December 6, 2002
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)