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Protein

Calreticulin

Gene

Crc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109CarbohydrateBy similarity1
Binding sitei111CarbohydrateBy similarity1
Binding sitei128CarbohydrateBy similarity1
Binding sitei135CarbohydrateBy similarity1
Binding sitei317CarbohydrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • central nervous system development Source: FlyBase
  • olfactory behavior Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • protein folding Source: InterPro
  • sleep Source: FlyBase

Keywordsi

Molecular functionChaperone
LigandCalcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Gene namesi
Name:Crc
ORF Names:CG9429
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0005585. Crc.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000000418218 – 406CalreticulinAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi105 ↔ 137By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP29413.
PRIDEiP29413.

Expressioni

Gene expression databases

BgeeiFBgn0005585.
ExpressionAtlasiP29413. differential.
GenevisibleiP29413. DM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi66325. 93 interactors.
DIPiDIP-19046N.
IntActiP29413. 3 interactors.
MINTiMINT-755481.
STRINGi7227.FBpp0081581.

Structurei

3D structure databases

ProteinModelPortaliP29413.
SMRiP29413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati191 – 2021-1Add BLAST12
Repeati210 – 2211-2Add BLAST12
Repeati227 – 2381-3Add BLAST12
Repeati244 – 2551-4Add BLAST12
Repeati259 – 2692-1Add BLAST11
Repeati273 – 2832-2Add BLAST11
Repeati287 – 2972-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 2554 X approximate repeatsAdd BLAST65
Regioni259 – 2973 X approximate repeatsAdd BLAST39

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 402Asp/Glu/Lys-richAdd BLAST50

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
InParanoidiP29413.
KOiK08057.
OMAiDTWIYSK.
OrthoDBiEOG091G0AP1.
PhylomeDBiP29413.

Family and domain databases

InterProiView protein in InterPro
IPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom_sf.
IPR013320. ConA-like_dom_sf.
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiView protein in Pfam
PF00262. Calreticulin. 2 hits.
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiView protein in PROSITE
PS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
PS00014. ER_TARGET. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMWCKTVIVL LATVGFISAE VYLKENFDNE NWEDTWIYSK HPGKEFGKFV
60 70 80 90 100
LTPGTFYNDA EADKGIQTSQ DARFYAASRK FDGFSNEDKP LVVQFSVKHE
110 120 130 140 150
QNIDCGGGYV KLFDCSLDQT DMHGESPYEI MFGPDICGPG TKKVHVIFSY
160 170 180 190 200
KGKNHLISKD IRCKDDVYTH FYTLIVRPDN TYEVLIDNEK VESGNLEDDW
210 220 230 240 250
DFLAPKKIKD PTATKPEDWD DRATIPDPDD KKPEDWDKPE HIPDPDATKP
260 270 280 290 300
EDWDDEMDGE WEPPMIDNPE FKGEWQPKQL DNPNYKGAWE HPEIANPEYV
310 320 330 340 350
PDDKLYLRKE ICTLGFDLWQ VKSGTIFDNV LITDDVELAA KAAAEVKNTQ
360 370 380 390 400
AGEKKMKEAQ DEVQRKKDEE EAKKASDKDD EDEDDDDEEK DDESKQDKDQ

SEHDEL
Length:406
Mass (Da):46,808
Last modified:October 1, 1993 - v2
Checksum:i65D72C69D0BEC427
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107G → A AA sequence (PubMed:2365822).Curated1
Sequence conflicti184V → L AA sequence (PubMed:2365822).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64461 Genomic DNA. Translation: CAA45791.1.
AE014297 Genomic DNA. Translation: AAF54416.1.
PIRiA56637.
RefSeqiNP_001262430.1. NM_001275501.1.
NP_524293.2. NM_079569.5.
UniGeneiDm.2457.

Genome annotation databases

EnsemblMetazoaiFBtr0082103; FBpp0081581; FBgn0005585.
FBtr0334618; FBpp0306685; FBgn0005585.
GeneIDi41166.
KEGGidme:Dmel_CG9429.

Similar proteinsi

Entry informationi

Entry nameiCALR_DROME
AccessioniPrimary (citable) accession number: P29413
Secondary accession number(s): Q9VHA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1993
Last modified: November 22, 2017
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families