Reviewed, UniProtKB/Swiss-Prot P29410 (KAD2_RAT)

Names and origin

Protein names

Recommended name:
    Adenylate kinase 2, mitochondrial
      Short name=AK 2
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 2

Gene names

Name:

Ak2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis By similarity.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion intermembrane space By similarity.
Sequence similarities	Belongs to the adenylate kinase family. AK2 subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Disulfide bond
Gene Ontology (GO)
Biological process	adenine metabolic process Ref.1 Traceable author statement. Source: RGD
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Traceable author statement. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P29410-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P29410-2)

The sequence of this isoform differs from the canonical sequence as follows:
     232-239: CKDLVMFV → S

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 239	238	Adenylate kinase 2, mitochondrial		PRO_0000158920
Regions
	Nucleotide binding	22 – 30	9	ATP By similarity
	Nucleotide binding	45 – 74	30	AMP By similarity
Sites
	Binding site	46	1	AMP By similarity
Amino acid modifications
	Modified residue	93	1	N6-acetyllysine By similarity
	Disulfide bond	42 ↔ 92		By similarity
Natural variations
	Alternative sequence	232 – 239	8	CKDLVMFV → S in isoform 2.		VSP_036505
Experimental info
	Sequence conflict	14	1	E → K in AAH61727. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 075A4ACBD581DB8C	FASTA	239	26,379
	10 20 30 40 50 60 MAPNALAPEP EHPEGIRAVL LGPPGAGKGT QAPKLAENFC VCHLATGDML RAMVASGSEL 70 80 90 100 110 120 GKKLKATMDA GKLVSDEMVV ELIEKNLETP SCKNGFLLDG FPRTVKQAEM LDDLMDKRKE 130 140 150 160 170 180 KLDSVIEFSI QDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EAMKDDITGE PLIRRSDDNE 190 200 210 220 230 KALKTRLEAY HTQTTPLVEY YRKRGIHCAI DASQTPDVVF ASILAAFSKA TCKDLVMFV
	Isoform 2. Checksum: 07537364EC6EEFA4	FASTA	232	25,530
	10 20 30 40 50 60 MAPNALAPEP EHPEGIRAVL LGPPGAGKGT QAPKLAENFC VCHLATGDML RAMVASGSEL 70 80 90 100 110 120 GKKLKATMDA GKLVSDEMVV ELIEKNLETP SCKNGFLLDG FPRTVKQAEM LDDLMDKRKE 130 140 150 160 170 180 KLDSVIEFSI QDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EAMKDDITGE PLIRRSDDNE 190 200 210 220 230 KALKTRLEAY HTQTTPLVEY YRKRGIHCAI DASQTPDVVF ASILAAFSKA TS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue-specific and developmentally regulated expression of the genes encoding adenylate kinase isozymes." Tanabe T., Yamada M., Noma T., Kajii T., Nakazawa A. J. Biochem. 113:200-207(1993) [PubMed: 8468325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Prostate.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13061 mRNA. Translation: BAA02378.1. BC061727 mRNA. Translation: AAH61727.1.
IPI	IPI00230857. IPI00923141.
PIR	JQ1944.
RefSeq	NP_001029139.1. NP_112248.1.
UniGene	Rn.3421
3D structure databases
HSSP	HSSP built from PDB template 1AK2 based on UniProtKB P08166.
SMR	P29410. Positions 15-232.
ModBase	Search...
PTM databases
PhosphoSite	P29410.
Proteomic databases
PRIDE	P29410.
Genome annotation databases
Ensembl	ENSRNOG00000000122. Rattus norvegicus. [Contig view]
GeneID	24184.
Organism-specific databases
RGD	2077. Ak2.
Phylogenomic databases
HOVERGEN	P29410.
Enzyme and pathway databases
BRENDA	2.7.4.3. 248.
Gene expression databases
GermOnline	ENSRNOG00000000122. Rattus norvegicus.
Family and domain databases
InterPro	IPR006259. Adenyl_kin_sub. IPR000850. Adenylate_kin. IPR007862. Adenylate_kinase_Znf_lid. [Graphical view]
PANTHER	PTHR23359. Adenylate_kin. 1 hit.
Pfam	PF00406. ADK. 1 hit. PF05191. ADK_lid. 1 hit. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
ProDom	PD000657. Adenylate_kin. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01351. adk. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602539.

Entry information

Entry name

KAD2_RAT

Accession

Primary (citable) accession number: P29410
Secondary accession number(s): Q6P7C6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)