P29409 (PGKH_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phosphoglycerate kinase, chloroplastic EC=2.7.2.3
Organism	Spinacia oleracea (Spinach)
Taxonomic identifier	3562 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	433 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 28	›28	Chloroplast Potential
	Chain	29 – 433	405	Phosphoglycerate kinase, chloroplastic		PRO_0000023891
Regions
	Nucleotide binding	384 – 387	4	ATP By similarity
	Region	52 – 54	3	Substrate binding By similarity
	Region	91 – 94	4	Substrate binding By similarity
Sites
	Binding site	68	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	233	1	ATP By similarity
	Binding site	324	1	ATP; via carbonyl oxygen By similarity
	Binding site	355	1	ATP By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P29409 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: D57AE12AA53099F9	FASTA	433	45,573
	10 20 30 40 50 60 GASFSLHVLS KINSYKSQST KPIRGVASMA KKSVGDLTSA DLKGKKVFVR ADLNVPLDDS 70 80 90 100 110 120 QNITDDTRIR AAIPTIKHLI NNGAKVILSS HLGRPKGVTP KFSLAPLVPR LSELLGLQVV 130 140 150 160 170 180 KADDCIGPDV EKLVAELPEG GVLLLENVRF YKEEEKNDPE FAKKLASLAD LYVNDAFGTA 190 200 210 220 230 240 HRAHASTEGV TKFLKPSVAG FLLQKELDYL VGAVSNPKRP FAAIVGGSKV SSKIGVIESL 250 260 270 280 290 300 LEKCDILLLG GGMIFTFYKA QGMSVGSSLV EEDKLDLATS LLAKAKEKGV SLLLPTDVVI 310 320 330 340 350 360 ADKFAADADS KIVPASGIPD GWMGLDIGPD SIKTFSEALD TTQTVIWNGP MGVFEFEKFA 370 380 390 400 410 420 AGTEAIAKKL EEISKKGATT IIGGGDSVAA VEKVGVAEAM SHISTGGGAS LELLEGKQLP 430 GVLALNEADP VPV

References

[1]	"Sequence of mature phosphoglycerate kinase from spinach chloroplasts." Bertsch U., Schlicher T.B., Schroeder I., Soll J. Plant Physiol. 103:1449-1450(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68430 mRNA. Translation: CAA48479.1.
PIR	S26623.
3D structure databases
ProteinModelPortal	P29409.
SMR	P29409. Positions 29-427.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P29409.
UniPathway	UPA00116.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKH_SPIOL

Accession

Primary (citable) accession number: P29409

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program