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P29409 (PGKH_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase, chloroplastic

EC=2.7.2.3
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length433 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate biosynthesis; Calvin cycle. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processCalvin cycle
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: InterPro

reductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 28›28Chloroplast Potential
Chain29 – 433405Phosphoglycerate kinase, chloroplastic HAMAP-Rule MF_00145
PRO_0000023891

Regions

Nucleotide binding384 – 3874ATP By similarity
Region52 – 543Substrate binding By similarity
Region91 – 944Substrate binding By similarity

Sites

Binding site681Substrate By similarity
Binding site1491Substrate By similarity
Binding site1821Substrate By similarity
Binding site2331ATP By similarity
Binding site3241ATP; via carbonyl oxygen By similarity
Binding site3551ATP By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P29409 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: D57AE12AA53099F9

FASTA43345,573
        10         20         30         40         50         60 
GASFSLHVLS KINSYKSQST KPIRGVASMA KKSVGDLTSA DLKGKKVFVR ADLNVPLDDS 

        70         80         90        100        110        120 
QNITDDTRIR AAIPTIKHLI NNGAKVILSS HLGRPKGVTP KFSLAPLVPR LSELLGLQVV 

       130        140        150        160        170        180 
KADDCIGPDV EKLVAELPEG GVLLLENVRF YKEEEKNDPE FAKKLASLAD LYVNDAFGTA 

       190        200        210        220        230        240 
HRAHASTEGV TKFLKPSVAG FLLQKELDYL VGAVSNPKRP FAAIVGGSKV SSKIGVIESL 

       250        260        270        280        290        300 
LEKCDILLLG GGMIFTFYKA QGMSVGSSLV EEDKLDLATS LLAKAKEKGV SLLLPTDVVI 

       310        320        330        340        350        360 
ADKFAADADS KIVPASGIPD GWMGLDIGPD SIKTFSEALD TTQTVIWNGP MGVFEFEKFA 

       370        380        390        400        410        420 
AGTEAIAKKL EEISKKGATT IIGGGDSVAA VEKVGVAEAM SHISTGGGAS LELLEGKQLP 

       430 
GVLALNEADP VPV 

« Hide

References

[1]"Sequence of mature phosphoglycerate kinase from spinach chloroplasts."
Bertsch U., Schlicher T.B., Schroeder I., Soll J.
Plant Physiol. 103:1449-1450(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68430 mRNA. Translation: CAA48479.1.
PIRS26623.

3D structure databases

ProteinModelPortalP29409.
SMRP29409. Positions 29-427.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP29409.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12711.
SABIO-RKP29409.
UniPathwayUPA00116.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKH_SPIOL
AccessionPrimary (citable) accession number: P29409
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways