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Protein

Phosphoglycerate kinase, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68SubstrateBy similarity1
Binding sitei149SubstrateBy similarity1
Binding sitei182SubstrateBy similarity1
Binding sitei233ATPBy similarity1
Binding sitei324ATP; via carbonyl oxygenBy similarity1
Binding sitei355ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi384 – 387ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12711.
SABIO-RKP29409.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase, chloroplastic (EC:2.7.2.3)
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei‹1 – 28ChloroplastSequence analysisAdd BLAST›28
ChainiPRO_000002389129 – 433Phosphoglycerate kinase, chloroplasticAdd BLAST405

Proteomic databases

PRIDEiP29409.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP29409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 54Substrate bindingBy similarity3
Regioni91 – 94Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GASFSLHVLS KINSYKSQST KPIRGVASMA KKSVGDLTSA DLKGKKVFVR
60 70 80 90 100
ADLNVPLDDS QNITDDTRIR AAIPTIKHLI NNGAKVILSS HLGRPKGVTP
110 120 130 140 150
KFSLAPLVPR LSELLGLQVV KADDCIGPDV EKLVAELPEG GVLLLENVRF
160 170 180 190 200
YKEEEKNDPE FAKKLASLAD LYVNDAFGTA HRAHASTEGV TKFLKPSVAG
210 220 230 240 250
FLLQKELDYL VGAVSNPKRP FAAIVGGSKV SSKIGVIESL LEKCDILLLG
260 270 280 290 300
GGMIFTFYKA QGMSVGSSLV EEDKLDLATS LLAKAKEKGV SLLLPTDVVI
310 320 330 340 350
ADKFAADADS KIVPASGIPD GWMGLDIGPD SIKTFSEALD TTQTVIWNGP
360 370 380 390 400
MGVFEFEKFA AGTEAIAKKL EEISKKGATT IIGGGDSVAA VEKVGVAEAM
410 420 430
SHISTGGGAS LELLEGKQLP GVLALNEADP VPV
Length:433
Mass (Da):45,573
Last modified:February 1, 1996 - v2
Checksum:iD57AE12AA53099F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68430 mRNA. Translation: CAA48479.1.
PIRiS26623.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68430 mRNA. Translation: CAA48479.1.
PIRiS26623.

3D structure databases

ProteinModelPortaliP29409.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP29409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00116.
BioCyciMetaCyc:MONOMER-12711.
SABIO-RKP29409.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGKH_SPIOL
AccessioniPrimary (citable) accession number: P29409
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: October 5, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.