P29398 (RPOB_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	TM_0458

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	1263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1263	1263	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047984

Sequences

	Sequence		Length	Mass (Da)
	P29398 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 04B79368567C8237	FASTA	1,263	143,138
	10 20 30 40 50 60 MKEISCGRRT RVSFGKSREP LPIPDLVEIQ KSSYRRFLEE GLLEVLKKFS PIYSQATRSD 70 80 90 100 110 120 LRKSDRGFAL EFVSTRTGEP AIDPLECKAK GLTYSVPIYA TARLTDMKSG EMKEEEVFLG 130 140 150 160 170 180 YIPYMTDRGT FIINGAERVV VNQIVVSPGL YFSSEYIDRE EYGGYFLPSR GAWLEVILDP 190 200 210 220 230 240 YDGVLYAGLD GKKVNLFLFL KTIGYEKDED ILSLYPTYLD ADDEDSLLLH VGSILLEDIY 250 260 270 280 290 300 DGGRKIAEKW DILTKDLAER ILMIDDINQI KIVHPIAQNT FEKMLEVVSS SSEEGEEEEE 310 320 330 340 350 360 KTKIYGLNEV TVVDAYLEIF RRLRPEELPR INAAKRYLHD LFFNPERYDL SEVGRYKVNE 370 380 390 400 410 420 RLRNAYIRYL IEVEGEDPEE ARKKVYNETS LVLKPLDIVL ASRILFDYFE RRYVNDFEID 430 440 450 460 470 480 SYELKNLIRI FKEEYLEKRK TAPYDLRKLV SVFRRNYGVT SDLGVFAAIR YVSNINKELP 490 500 510 520 530 540 SIPFDTKDHL GNKRVRTVGE LVQREFERLF ARAQKAIQER LTLINSLSKV SIQSLINIKS 550 560 570 580 590 600 IISTVNQFFA MNQLSQFMDQ VNPLSELTHK RRVSAVGPGG LRRESKVFEA RNVHYSQYGR 610 620 630 640 650 660 LCPIETPEGA NIGFITSLAI YAKIDEYGFL MTPYRKVVNG KVTDEVVYLR ANEEEEYKII 670 680 690 700 710 720 PATTPVDEEG NIIPERVVAR MGEDIRLVPK EEVDFMDVST KQPFSVSASL IPFLEHDDAS 730 740 750 760 770 780 RALMGSNMQR QAVPLLKTEA PLVGTGMEWE AAKNSGYVIL AEHDGIVKEV DAARVVVHRT 790 800 810 820 830 840 DENGNLMYDD KGNPVVDEYR LLKFVRSNQD TMINQKPIVN EGDFVKKGDP IADGPATDMG 850 860 870 880 890 900 ELALGRNILV AFMPWEGYNY EDAILVSQEL LEEDVFTSIH IEVYETQARE TRLGPEEITA 910 920 930 940 950 960 DIPNVSKELL KNLDENGIIR VGAYVVSDYG VGSQAILVGK VTPKGEGDTT PEEKIIRSVF 970 980 990 1000 1010 1020 GERGRDVKDT SLRLPHGVEG RVIRVDVYDQ NDIAELGAGV LKLVRVYVAS RKTLDIGDKL 1030 1040 1050 1060 1070 1080 AGRHGNKGVV SNILPKEDMP FLPDGTPVQM VLNPLGIPSR MNVGQILETH LGWLAKLTGK 1090 1100 1110 1120 1130 1140 WFATPVFEGA KEDEILRPLY EERKKRGLHL GDDENNPNGK VVLRDGRTGE PFDNPVVVGY 1150 1160 1170 1180 1190 1200 MYMLKLIHIA KEKIHARSTG PYSLIHQQPL GGKSHFGGQR LGEMEVWALE AYGAAHTLAE 1210 1220 1230 1240 1250 1260 MLTIKSDDIK GRNEAYKAIL KNMNIPEPGV PESFRVLIKE LRGLALDVRL YDENGNEIDI DKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits." Palm P., Schleper C., Arnold-Ammer I., Holz I., Meier T., Lottspeich F., Zillig W. Nucleic Acids Res. 21:4904-4908(1993) [PubMed: 8177738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]	"The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima." Liao D., Dennis P.P. J. Biol. Chem. 267:22787-22797(1992) [PubMed: 1429627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-404. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72695 Genomic DNA. Translation: CAA51246.1. AE000512 Genomic DNA. Translation: AAD35543.1. Z11839 Genomic DNA. Translation: CAA77863.1.
PIR	F44466. S41466.
RefSeq	NP_228268.1. NC_000853.1.
3D structure databases
ProteinModelPortal	P29398.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897488.
GenomeReviews	Gene locus TM_0458 in contig AE000512_GR.
KEGG	tma:TM0458.
NMPDR	fig|243274.1.peg.452.
PATRIC	23935813. VBITheMar51294_0465.
TIGR	TM_0458.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	HANEEIT.
PhylomeDB	P29398.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0458-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_THEMA

Accession

Primary (citable) accession number: P29398

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program