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P29397

- NUSG_THEMA

UniProt

P29397 - NUSG_THEMA

Protein

Transcription termination/antitermination protein NusG

Gene

nusG

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Participates in transcription elongation, termination and antitermination.UniRule annotation

    Enzyme regulationi

    Regulated by autoinhibition via interaction of the N-terminal and the C-terminal domains. Autoinhibition may prevent NusG from interacting prematurely with other components of the transcription complex or non-specific interactions with other cellular components.1 Publication

    GO - Biological processi

    1. DNA-templated transcription, elongation Source: UniProtKB-HAMAP
    2. DNA-templated transcription, termination Source: UniProtKB-HAMAP
    3. regulation of DNA-templated transcription, elongation Source: InterPro
    4. transcription antitermination Source: UniProtKB-HAMAP

    Keywords - Biological processi

    Transcription, Transcription antitermination, Transcription regulation, Transcription termination

    Enzyme and pathway databases

    BioCyciTMAR243274:GC6P-473-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription termination/antitermination protein NusGUniRule annotation
    Gene namesi
    Name:nusGUniRule annotation
    Ordered Locus Names:TM_0453
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 353353Transcription termination/antitermination protein NusGPRO_0000113965Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243274.TM0453.

    Structurei

    Secondary structure

    1
    353
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 96
    Helixi15 – 2915
    Beta strandi33 – 397
    Beta strandi42 – 465
    Beta strandi52 – 576
    Beta strandi62 – 643
    Beta strandi76 – 805
    Beta strandi89 – 10315
    Beta strandi110 – 1167
    Helixi117 – 1193
    Beta strandi135 – 1384
    Beta strandi147 – 16317
    Beta strandi169 – 1757
    Helixi176 – 1783
    Turni181 – 1833
    Beta strandi195 – 1973
    Beta strandi200 – 2034
    Beta strandi208 – 2147
    Beta strandi219 – 2246
    Beta strandi226 – 2327
    Beta strandi235 – 2406
    Helixi244 – 2518
    Beta strandi262 – 2654
    Helixi271 – 28010
    Beta strandi304 – 3074
    Turni311 – 3144
    Beta strandi316 – 3238
    Turni324 – 3274
    Beta strandi328 – 3358
    Beta strandi338 – 3458
    Helixi346 – 3483

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LQ8NMR-A1-41[»]
    A230-300[»]
    2XHAX-ray1.91A/B41-233[»]
    2XHCX-ray2.45A1-352[»]
    ProteinModelPortaliP29397.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29397.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini301 – 33535KOWUniRule annotationAdd
    BLAST

    Domaini

    The N-terminal domain interacts with RNAP and the C-terminal domain binds either to Rho or to RpsJ (NusE). Contains an additional, species-specific domain inserted into the N-terminal domain. The N-terminal and C-terminal domains can interact and form a closed conformation.1 Publication

    Sequence similaritiesi

    Belongs to the NusG family.UniRule annotation
    Contains 1 KOW domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0250.
    KOiK02601.
    OMAiYLFVEMI.
    OrthoDBiEOG6FFS95.

    Family and domain databases

    Gene3Di2.30.30.30. 1 hit.
    3.30.70.940. 2 hits.
    HAMAPiMF_00948. NusG.
    InterProiIPR005824. KOW.
    IPR006645. NGN_dom.
    IPR014722. Rib_L2_dom2.
    IPR001062. Transcrpt_antiterm_NusG.
    IPR015869. Transcrpt_antiterm_NusG_bac_CS.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view]
    PfamiPF00467. KOW. 1 hit.
    PF02357. NusG. 1 hit.
    [Graphical view]
    PRINTSiPR00338. NUSGTNSCPFCT.
    SMARTiSM00739. KOW. 1 hit.
    SM00738. NGN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50104. SSF50104. 1 hit.
    SSF82679. SSF82679. 2 hits.
    TIGRFAMsiTIGR00922. nusG. 1 hit.
    PROSITEiPS01014. NUSG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29397-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKWYIVLT MSGYEEKVKE NIEKKVEATG IKNLVGRIVI PEEVVLDATS    50
    PSERLILSPK AKLHVNNGKD VNKGDLIAEE PPIYARRSGV IVDVKNVRKI 100
    VVETIDRKYT KTYYIPESAG IEPGLRVGTK VKQGLPLSKN EEYICELDGK 150
    IVEIERMKKV VVQTPDGEQD VYYIPLDVFD RDRIKKGKEV KQGEMLAEAR 200
    KFFAKVSGRV EVVDYSTRKE IRIYKTKRRK LFPGYVFVEM IMNDEAYNFV 250
    RSVPYVMGFV SSGGQPVPVK DREMRPILRL AGLEEYEEKK KPVKVELGFK 300
    VGDMVKIISG PFEDFAGVIK EIDPERQELK VNVTIFGRET PVVLHVSEVE 350
    KIE 353
    Length:353
    Mass (Da):40,327
    Last modified:December 1, 1992 - v1
    Checksum:i98DCB44B2E2CABB5
    GO

    Sequence cautioni

    The sequence AAD35536.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11839 Genomic DNA. Translation: CAA77858.1.
    AE000512 Genomic DNA. Translation: AAD35536.1. Different initiation.
    PIRiF72375.
    RefSeqiNP_228263.1. NC_000853.1.
    YP_007976800.1. NC_021214.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35536; AAD35536; TM_0453.
    GeneIDi897482.
    KEGGitma:TM0453.
    tmm:Tmari_0450.
    PATRICi23935801. VBITheMar51294_0459.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11839 Genomic DNA. Translation: CAA77858.1 .
    AE000512 Genomic DNA. Translation: AAD35536.1 . Different initiation.
    PIRi F72375.
    RefSeqi NP_228263.1. NC_000853.1.
    YP_007976800.1. NC_021214.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LQ8 NMR - A 1-41 [» ]
    A 230-300 [» ]
    2XHA X-ray 1.91 A/B 41-233 [» ]
    2XHC X-ray 2.45 A 1-352 [» ]
    ProteinModelPortali P29397.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0453.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35536 ; AAD35536 ; TM_0453 .
    GeneIDi 897482.
    KEGGi tma:TM0453.
    tmm:Tmari_0450.
    PATRICi 23935801. VBITheMar51294_0459.

    Phylogenomic databases

    eggNOGi COG0250.
    KOi K02601.
    OMAi YLFVEMI.
    OrthoDBi EOG6FFS95.

    Enzyme and pathway databases

    BioCyci TMAR243274:GC6P-473-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P29397.

    Family and domain databases

    Gene3Di 2.30.30.30. 1 hit.
    3.30.70.940. 2 hits.
    HAMAPi MF_00948. NusG.
    InterProi IPR005824. KOW.
    IPR006645. NGN_dom.
    IPR014722. Rib_L2_dom2.
    IPR001062. Transcrpt_antiterm_NusG.
    IPR015869. Transcrpt_antiterm_NusG_bac_CS.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view ]
    Pfami PF00467. KOW. 1 hit.
    PF02357. NusG. 1 hit.
    [Graphical view ]
    PRINTSi PR00338. NUSGTNSCPFCT.
    SMARTi SM00739. KOW. 1 hit.
    SM00738. NGN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50104. SSF50104. 1 hit.
    SSF82679. SSF82679. 2 hits.
    TIGRFAMsi TIGR00922. nusG. 1 hit.
    PROSITEi PS01014. NUSG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima."
      Liao D., Dennis P.P.
      J. Biol. Chem. 267:22787-22797(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. "Crystal structure of the antitermination factor Nusg from Thermotoga maritima."
      Stegmann C.M., Mandal A., Schweimer C., Burmann B., Roesch P., Wahl M.C.
      Submitted (JUN-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 1-352.
    4. Cited for: STRUCTURE BY NMR, ENZYME REGULATION, DOMAIN.

    Entry informationi

    Entry nameiNUSG_THEMA
    AccessioniPrimary (citable) accession number: P29397
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3