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Protein

Transcription termination/antitermination protein NusG

Gene

nusG

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in transcription elongation, termination and antitermination.UniRule annotation

Enzyme regulationi

Regulated by autoinhibition via interaction of the N-terminal and the C-terminal domains. Autoinhibition may prevent NusG from interacting prematurely with other components of the transcription complex or non-specific interactions with other cellular components.1 Publication

GO - Biological processi

  1. DNA-templated transcription, elongation Source: UniProtKB-HAMAP
  2. DNA-templated transcription, termination Source: UniProtKB-HAMAP
  3. regulation of DNA-templated transcription, elongation Source: InterPro
  4. transcription antitermination Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription antitermination, Transcription regulation, Transcription termination

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-473-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination/antitermination protein NusGUniRule annotation
Gene namesi
Name:nusGUniRule annotation
Ordered Locus Names:TM_0453
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Transcription termination/antitermination protein NusGPRO_0000113965Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0453.

Structurei

Secondary structure

1
353
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi15 – 2915Combined sources
Beta strandi33 – 397Combined sources
Beta strandi42 – 465Combined sources
Beta strandi52 – 576Combined sources
Beta strandi62 – 643Combined sources
Beta strandi76 – 805Combined sources
Beta strandi89 – 10315Combined sources
Beta strandi110 – 1167Combined sources
Helixi117 – 1193Combined sources
Beta strandi135 – 1384Combined sources
Beta strandi147 – 16317Combined sources
Beta strandi169 – 1757Combined sources
Helixi176 – 1783Combined sources
Turni181 – 1833Combined sources
Beta strandi195 – 1973Combined sources
Beta strandi200 – 2034Combined sources
Beta strandi208 – 2147Combined sources
Beta strandi219 – 2246Combined sources
Beta strandi226 – 2327Combined sources
Beta strandi235 – 2406Combined sources
Helixi244 – 2518Combined sources
Beta strandi262 – 2654Combined sources
Helixi271 – 28010Combined sources
Beta strandi304 – 3074Combined sources
Turni311 – 3144Combined sources
Beta strandi316 – 3238Combined sources
Turni324 – 3274Combined sources
Beta strandi328 – 3358Combined sources
Beta strandi338 – 3458Combined sources
Helixi346 – 3483Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LQ8NMR-A1-41[»]
A230-300[»]
2XHAX-ray1.91A/B41-233[»]
2XHCX-ray2.45A1-352[»]
ProteinModelPortaliP29397.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29397.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini301 – 33535KOWUniRule annotationAdd
BLAST

Domaini

The N-terminal domain interacts with RNAP and the C-terminal domain binds either to Rho or to RpsJ (NusE). Contains an additional, species-specific domain inserted into the N-terminal domain. The N-terminal and C-terminal domains can interact and form a closed conformation.1 Publication

Sequence similaritiesi

Belongs to the NusG family.UniRule annotation
Contains 1 KOW domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0250.
InParanoidiP29397.
KOiK02601.
OMAiYLFVEMI.
OrthoDBiEOG6FFS95.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
3.30.70.940. 2 hits.
HAMAPiMF_00948. NusG.
InterProiIPR005824. KOW.
IPR006645. NGN_dom.
IPR014722. Rib_L2_dom2.
IPR001062. Transcrpt_antiterm_NusG.
IPR015869. Transcrpt_antiterm_NusG_bac_CS.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF00467. KOW. 1 hit.
PF02357. NusG. 1 hit.
[Graphical view]
PRINTSiPR00338. NUSGTNSCPFCT.
SMARTiSM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF82679. SSF82679. 2 hits.
TIGRFAMsiTIGR00922. nusG. 1 hit.
PROSITEiPS01014. NUSG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKWYIVLT MSGYEEKVKE NIEKKVEATG IKNLVGRIVI PEEVVLDATS
60 70 80 90 100
PSERLILSPK AKLHVNNGKD VNKGDLIAEE PPIYARRSGV IVDVKNVRKI
110 120 130 140 150
VVETIDRKYT KTYYIPESAG IEPGLRVGTK VKQGLPLSKN EEYICELDGK
160 170 180 190 200
IVEIERMKKV VVQTPDGEQD VYYIPLDVFD RDRIKKGKEV KQGEMLAEAR
210 220 230 240 250
KFFAKVSGRV EVVDYSTRKE IRIYKTKRRK LFPGYVFVEM IMNDEAYNFV
260 270 280 290 300
RSVPYVMGFV SSGGQPVPVK DREMRPILRL AGLEEYEEKK KPVKVELGFK
310 320 330 340 350
VGDMVKIISG PFEDFAGVIK EIDPERQELK VNVTIFGRET PVVLHVSEVE

KIE
Length:353
Mass (Da):40,327
Last modified:December 1, 1992 - v1
Checksum:i98DCB44B2E2CABB5
GO

Sequence cautioni

The sequence AAD35536.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11839 Genomic DNA. Translation: CAA77858.1.
AE000512 Genomic DNA. Translation: AAD35536.1. Different initiation.
PIRiF72375.
RefSeqiNP_228263.1. NC_000853.1.
YP_007976800.1. NC_021214.1.

Genome annotation databases

EnsemblBacteriaiAAD35536; AAD35536; TM_0453.
GeneIDi897482.
KEGGitma:TM0453.
PATRICi23935801. VBITheMar51294_0459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11839 Genomic DNA. Translation: CAA77858.1.
AE000512 Genomic DNA. Translation: AAD35536.1. Different initiation.
PIRiF72375.
RefSeqiNP_228263.1. NC_000853.1.
YP_007976800.1. NC_021214.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LQ8NMR-A1-41[»]
A230-300[»]
2XHAX-ray1.91A/B41-233[»]
2XHCX-ray2.45A1-352[»]
ProteinModelPortaliP29397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35536; AAD35536; TM_0453.
GeneIDi897482.
KEGGitma:TM0453.
PATRICi23935801. VBITheMar51294_0459.

Phylogenomic databases

eggNOGiCOG0250.
InParanoidiP29397.
KOiK02601.
OMAiYLFVEMI.
OrthoDBiEOG6FFS95.

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-473-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP29397.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
3.30.70.940. 2 hits.
HAMAPiMF_00948. NusG.
InterProiIPR005824. KOW.
IPR006645. NGN_dom.
IPR014722. Rib_L2_dom2.
IPR001062. Transcrpt_antiterm_NusG.
IPR015869. Transcrpt_antiterm_NusG_bac_CS.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF00467. KOW. 1 hit.
PF02357. NusG. 1 hit.
[Graphical view]
PRINTSiPR00338. NUSGTNSCPFCT.
SMARTiSM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF82679. SSF82679. 2 hits.
TIGRFAMsiTIGR00922. nusG. 1 hit.
PROSITEiPS01014. NUSG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima."
    Liao D., Dennis P.P.
    J. Biol. Chem. 267:22787-22797(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "Crystal structure of the antitermination factor Nusg from Thermotoga maritima."
    Stegmann C.M., Mandal A., Schweimer C., Burmann B., Roesch P., Wahl M.C.
    Submitted (MAY-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 1-352.
  4. Cited for: STRUCTURE BY NMR, ENZYME REGULATION, DOMAIN.

Entry informationi

Entry nameiNUSG_THEMA
AccessioniPrimary (citable) accession number: P29397
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 1, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.