P29397 (NUSG_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription termination/antitermination protein NusG

Gene names

Name:	nusG
Ordered Locus Names:	TM_0453

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in transcription elongation, termination and antitermination By similarity. HAMAP-Rule MF_00948
Enzyme regulation	Regulated by autoinhibition via interaction of the N-terminal and the C-terminal domains. Autoinhibition may prevent NusG from interacting prematurely with other components of the transcription complex or non-specific interactions with other cellular components. Ref.4
Domain	The N-terminal domain interacts with RNAP and the C-terminal domain binds either to Rho or to RpsJ (NusE). Contains an additional, species-specific domain inserted into the N-terminal domain. The N-terminal and C-terminal domains can interact and form a closed conformation. Ref.4
Sequence similarities	Belongs to the NusG family. Contains 1 KOW domain.
Sequence caution	The sequence AAD35536.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription antitermination Transcription regulation Transcription termination
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, termination Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of transcription elongation from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro transcription antitermination Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 353

353

Transcription termination/antitermination protein NusG HAMAP-Rule MF_00948

PRO_0000113965

Regions

Domain

301 – 335

35

KOW

Secondary structure

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353

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Helix

15 – 29

15

Beta strand

33 – 39

7

Beta strand

42 – 46

5

Beta strand

52 – 57

6

Beta strand

62 – 64

3

Beta strand

76 – 80

5

Beta strand

89 – 103

15

Beta strand

110 – 116

7

Helix

117 – 119

3

Beta strand

135 – 138

4

Beta strand

147 – 163

17

Beta strand

169 – 175

7

Helix

176 – 178

3

Turn

181 – 183

3

Beta strand

195 – 197

3

Beta strand

200 – 203

4

Beta strand

208 – 214

7

Beta strand

219 – 224

6

Beta strand

226 – 232

7

Beta strand

235 – 240

6

Helix

244 – 251

8

Beta strand

262 – 265

4

Helix

271 – 280

10

Beta strand

304 – 307

4

Turn

311 – 314

4

Beta strand

316 – 323

8

Turn

324 – 327

4

Beta strand

328 – 335

8

Beta strand

338 – 345

8

Helix

346 – 348

3

Sequences

	Sequence		Length	Mass (Da)
	P29397 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 98DCB44B2E2CABB5	FASTA	353	40,327
	10 20 30 40 50 60 MKKKWYIVLT MSGYEEKVKE NIEKKVEATG IKNLVGRIVI PEEVVLDATS PSERLILSPK 70 80 90 100 110 120 AKLHVNNGKD VNKGDLIAEE PPIYARRSGV IVDVKNVRKI VVETIDRKYT KTYYIPESAG 130 140 150 160 170 180 IEPGLRVGTK VKQGLPLSKN EEYICELDGK IVEIERMKKV VVQTPDGEQD VYYIPLDVFD 190 200 210 220 230 240 RDRIKKGKEV KQGEMLAEAR KFFAKVSGRV EVVDYSTRKE IRIYKTKRRK LFPGYVFVEM 250 260 270 280 290 300 IMNDEAYNFV RSVPYVMGFV SSGGQPVPVK DREMRPILRL AGLEEYEEKK KPVKVELGFK 310 320 330 340 350 VGDMVKIISG PFEDFAGVIK EIDPERQELK VNVTIFGRET PVVLHVSEVE KIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima." Liao D., Dennis P.P. J. Biol. Chem. 267:22787-22797(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]	"Crystal structure of the antitermination factor Nusg from Thermotoga maritima." Stegmann C.M., Mandal A., Schweimer C., Burmann B., Roesch P., Wahl M.C. Submitted (JUN-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 1-352.
[4]	"An autoinhibited state in the structure of Thermotoga maritima NusG." Drogemuller J., Stegmann C.M., Mandal A., Steiner T., Burmann B.M., Gottesman M.E., Wohrl B.M., Rosch P., Wahl M.C., Schweimer K. Structure 0:0-0(2013) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, ENZYME REGULATION, DOMAIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z11839 Genomic DNA. Translation: CAA77858.1.
AE000512 Genomic DNA. Translation: AAD35536.1. Different initiation.

PIR

F72375.

RefSeq

NP_228263.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LQ8	NMR	-	A	1-353	[»]
2XHA	X-ray	1.91	A/B	41-233	[»]
2XHC	X-ray	2.45	A	1-352	[»]

ProteinModelPortal

P29397.

ModBase

Search...

Protein-protein interaction databases

STRING

243274.TM0453.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD35536; AAD35536; TM_0453.

GeneID

897482.

KEGG

tma:TM0453.

PATRIC

23935801. VBITheMar51294_0459.

Phylogenomic databases

eggNOG

COG0250.

KO

K02601.

OMA

YLFVEMI.

ProtClustDB

CLSK875151.

Family and domain databases

Gene3D

2.30.30.30. 1 hit.
3.30.70.940. 2 hits.

HAMAP

MF_00948. NusG.

InterPro

IPR005824. KOW.
IPR014722. Rib_L2_dom2.
IPR001062. Transcrpt_antiterm_NusG.
IPR015869. Transcrpt_antiterm_NusG_bac_CS.
IPR006645. Transcrpt_antiterm_NusG_N.
IPR008991. Translation_prot_SH3-like.
[Graphical view]

Pfam

PF00467. KOW. 1 hit.
PF02357. NusG. 1 hit.
[Graphical view]

PRINTS

PR00338. NUSGTNSCPFCT.

SMART

SM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]

SUPFAM

SSF82679. Transcrpt_antiterm_NusG_N. 1 hit.
SSF50104. Transl_SH3_like. 1 hit.

TIGRFAMs

TIGR00922. nusG. 1 hit.

PROSITE

PS01014. NUSG. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P29397.

Entry information

Entry name

NUSG_THEMA

Accession

Primary (citable) accession number: P29397

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program