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P29397 (NUSG_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription termination/antitermination protein NusG
Gene names
Name:nusG
Ordered Locus Names:TM_0453
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in transcription elongation, termination and antitermination By similarity. HAMAP-Rule MF_00948

Enzyme regulation

Regulated by autoinhibition via interaction of the N-terminal and the C-terminal domains. Autoinhibition may prevent NusG from interacting prematurely with other components of the transcription complex or non-specific interactions with other cellular components. Ref.4

Domain

The N-terminal domain interacts with RNAP and the C-terminal domain binds either to Rho or to RpsJ (NusE). Contains an additional, species-specific domain inserted into the N-terminal domain. The N-terminal and C-terminal domains can interact and form a closed conformation. Ref.4

Sequence similarities

Belongs to the NusG family.

Contains 1 KOW domain.

Sequence caution

The sequence AAD35536.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Transcription termination/antitermination protein NusG HAMAP-Rule MF_00948
PRO_0000113965

Regions

Domain301 – 33535KOW

Secondary structure

.......................................................... 353
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29397 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 98DCB44B2E2CABB5

FASTA35340,327
        10         20         30         40         50         60 
MKKKWYIVLT MSGYEEKVKE NIEKKVEATG IKNLVGRIVI PEEVVLDATS PSERLILSPK 

        70         80         90        100        110        120 
AKLHVNNGKD VNKGDLIAEE PPIYARRSGV IVDVKNVRKI VVETIDRKYT KTYYIPESAG 

       130        140        150        160        170        180 
IEPGLRVGTK VKQGLPLSKN EEYICELDGK IVEIERMKKV VVQTPDGEQD VYYIPLDVFD 

       190        200        210        220        230        240 
RDRIKKGKEV KQGEMLAEAR KFFAKVSGRV EVVDYSTRKE IRIYKTKRRK LFPGYVFVEM 

       250        260        270        280        290        300 
IMNDEAYNFV RSVPYVMGFV SSGGQPVPVK DREMRPILRL AGLEEYEEKK KPVKVELGFK 

       310        320        330        340        350 
VGDMVKIISG PFEDFAGVIK EIDPERQELK VNVTIFGRET PVVLHVSEVE KIE 

« Hide

References

« Hide 'large scale' references
[1]"The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima."
Liao D., Dennis P.P.
J. Biol. Chem. 267:22787-22797(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]"Crystal structure of the antitermination factor Nusg from Thermotoga maritima."
Stegmann C.M., Mandal A., Schweimer C., Burmann B., Roesch P., Wahl M.C.
Submitted (JUN-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 1-352.
[4]"An autoinhibited state in the structure of Thermotoga maritima NusG."
Drogemuller J., Stegmann C.M., Mandal A., Steiner T., Burmann B.M., Gottesman M.E., Wohrl B.M., Rosch P., Wahl M.C., Schweimer K.
Structure 21:365-375(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, ENZYME REGULATION, DOMAIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11839 Genomic DNA. Translation: CAA77858.1.
AE000512 Genomic DNA. Translation: AAD35536.1. Different initiation.
PIRF72375.
RefSeqNP_228263.1. NC_000853.1.
YP_007976800.1. NC_021214.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LQ8NMR-A1-353[»]
2XHAX-ray1.91A/B41-233[»]
2XHCX-ray2.45A1-352[»]
ProteinModelPortalP29397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0453.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35536; AAD35536; TM_0453.
GeneID15494053.
897482.
KEGGtma:TM0453.
tmm:Tmari_0450.
PATRIC23935801. VBITheMar51294_0459.

Phylogenomic databases

eggNOGCOG0250.
KOK02601.
OMAYLFVEMI.
OrthoDBEOG6FFS95.
ProtClustDBCLSK875151.

Enzyme and pathway databases

BioCycTMAR243274:GC6P-473-MONOMER.

Family and domain databases

Gene3D2.30.30.30. 1 hit.
3.30.70.940. 2 hits.
HAMAPMF_00948. NusG.
InterProIPR005824. KOW.
IPR014722. Rib_L2_dom2.
IPR001062. Transcrpt_antiterm_NusG.
IPR015869. Transcrpt_antiterm_NusG_bac_CS.
IPR006645. Transcrpt_antiterm_NusG_N_dom.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamPF00467. KOW. 1 hit.
PF02357. NusG. 1 hit.
[Graphical view]
PRINTSPR00338. NUSGTNSCPFCT.
SMARTSM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]
SUPFAMSSF50104. SSF50104. 1 hit.
SSF82679. SSF82679. 2 hits.
TIGRFAMsTIGR00922. nusG. 1 hit.
PROSITEPS01014. NUSG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP29397.

Entry information

Entry nameNUSG_THEMA
AccessionPrimary (citable) accession number: P29397
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: February 19, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references