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Protein

50S ribosomal protein L10

Gene

rplJ

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors (such as IF-2, EF-Tu, EF-G and RF3).Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L10
Gene namesi
Name:rplJ
Ordered Locus Names:TM_0456
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17917950S ribosomal protein L10PRO_0000154734Add
BLAST

Interactioni

Subunit structurei

Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and 23S rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which 3 L12 dimers bind in a sequential fashion forming a heptameric L10(L12)2(L12)2(L12)2 complex.2 Publications

Protein-protein interaction databases

STRINGi243274.TM0456.

Structurei

Secondary structure

1
179
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815Combined sources
Beta strandi22 – 276Combined sources
Helixi34 – 4815Combined sources
Beta strandi51 – 577Combined sources
Helixi59 – 6810Combined sources
Helixi75 – 773Combined sources
Beta strandi79 – 9012Combined sources
Helixi93 – 10513Combined sources
Helixi110 – 1123Combined sources
Beta strandi113 – 1186Combined sources
Beta strandi121 – 1244Combined sources
Helixi125 – 1328Combined sources
Helixi137 – 17539Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZAVX-ray1.90A1-179[»]
1ZAWX-ray2.30A1-179[»]
1ZAXX-ray2.10A1-179[»]
ProteinModelPortaliP29394.
SMRiP29394. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29394.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 17943Binds L7/L12 dimersAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein L10P family.Curated

Phylogenomic databases

eggNOGiENOG41081WB. Bacteria.
COG0244. LUCA.
InParanoidiP29394.
KOiK02864.
OMAiVVVAHYS.
OrthoDBiEOG6DNTDR.

Family and domain databases

HAMAPiMF_00362. Ribosomal_L10.
InterProiIPR022973. Ribosomal_L10.
IPR002363. Ribosomal_L10_eubac_CS.
IPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]
PROSITEiPS01109. RIBOSOMAL_L10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTRQQKELI VKEMSEIFKK TSLILFADFL GFTVADLTEL RSRLREKYGD
60 70 80 90 100
GARFRVVKNT LLNLALKNAE YEGYEEFLKG PTAVLYVTEG DPVEAVKIIY
110 120 130 140 150
NFYKDKKADL SRLKGGFLEG KKFTAEEVEN IAKLPSKEEL YAMLVGRVKA
160 170
PITGLVFALS GILRNLVYVL NAIKEKKSE
Length:179
Mass (Da):20,308
Last modified:February 1, 1996 - v2
Checksum:i497C5764A64FC7F9
GO

Mass spectrometryi

Molecular mass is 101376±8 Da from positions 2 - 128. Determined by ESI. Isolated L10(L12)6.1 Publication
Molecular mass is 20335±3 Da from positions 1 - 179. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11839 Genomic DNA. Translation: CAA77861.1.
AE000512 Genomic DNA. Translation: AAD35539.1.
X72695 Genomic DNA. Translation: CAA51244.1.
PIRiA72376. R5HG10.
RefSeqiNP_228266.1. NC_000853.1.
WP_004081510.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35539; AAD35539; TM_0456.
GeneIDi897486.
KEGGitma:TM0456.
PATRICi23935809. VBITheMar51294_0463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11839 Genomic DNA. Translation: CAA77861.1.
AE000512 Genomic DNA. Translation: AAD35539.1.
X72695 Genomic DNA. Translation: CAA51244.1.
PIRiA72376. R5HG10.
RefSeqiNP_228266.1. NC_000853.1.
WP_004081510.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZAVX-ray1.90A1-179[»]
1ZAWX-ray2.30A1-179[»]
1ZAXX-ray2.10A1-179[»]
ProteinModelPortaliP29394.
SMRiP29394. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35539; AAD35539; TM_0456.
GeneIDi897486.
KEGGitma:TM0456.
PATRICi23935809. VBITheMar51294_0463.

Phylogenomic databases

eggNOGiENOG41081WB. Bacteria.
COG0244. LUCA.
InParanoidiP29394.
KOiK02864.
OMAiVVVAHYS.
OrthoDBiEOG6DNTDR.

Miscellaneous databases

EvolutionaryTraceiP29394.

Family and domain databases

HAMAPiMF_00362. Ribosomal_L10.
InterProiIPR022973. Ribosomal_L10.
IPR002363. Ribosomal_L10_eubac_CS.
IPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]
PROSITEiPS01109. RIBOSOMAL_L10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium Thermotoga maritima."
    Liao D., Dennis P.P.
    J. Biol. Chem. 267:22787-22797(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits."
    Palm P., Schleper C., Arnold-Ammer I., Holz I., Meier T., Lottspeich F., Zillig W.
    Nucleic Acids Res. 21:4904-4908(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-179.
  4. "Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria."
    Ilag L.L., Videler H., McKay A.R., Sobott F., Fucini P., Nierhaus K.H., Robinson C.V.
    Proc. Natl. Acad. Sci. U.S.A. 102:8192-8197(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, STOICHIOMETRY, MASS SPECTROMETRY.
  5. "Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation."
    Diaconu M., Kothe U., Schlunzen F., Fischer N., Harms J.M., Tonevitsky A.G., Stark H., Rodnina M.V., Wahl M.C.
    Cell 121:991-1004(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), INTERACTION WITH L7/L12 (RPLL), SUBUNIT.

Entry informationi

Entry nameiRL10_THEMA
AccessioniPrimary (citable) accession number: P29394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1996
Last modified: April 13, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.