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Protein

Spermadhesin-1

Gene

SPADH1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates cell division and progesterone secretion of bovine granulosa cells in vitro in a potent and dose dependent manner. This protein appears to be a potent growth factor with effects on ovarian granulosa cells.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Spermadhesin-1
Alternative name(s):
Acidic seminal fluid protein
Short name:
ASFP
Gene namesi
Name:SPADH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 134114Spermadhesin-1PRO_0000033187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 51PROSITE-ProRule annotation1 Publication
Disulfide bondi74 ↔ 95PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP29392.

Expressioni

Tissue specificityi

Seminal vesicle tissue, ampulla and weakly in tissue of epididymis.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000014297.

Structurei

Secondary structure

1
134
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 343Combined sources
Beta strandi37 – 426Combined sources
Beta strandi49 – 568Combined sources
Beta strandi62 – 7110Combined sources
Turni74 – 763Combined sources
Beta strandi77 – 859Combined sources
Beta strandi90 – 10314Combined sources
Beta strandi105 – 11511Combined sources
Beta strandi125 – 1328Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SFPX-ray1.90A21-134[»]
ProteinModelPortaliP29392.
SMRiP29392. Positions 24-134.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29392.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 131102CUBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the spermadhesin family.Curated
Contains 1 CUB domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JGFV. Eukaryota.
ENOG41118AQ. LUCA.
HOGENOMiHOG000168451.
HOVERGENiHBG004277.
InParanoidiP29392.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR000124. Spermadhesin.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00985. SPERMADHESIN_1. 1 hit.
PS00986. SPERMADHESIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSSVIPWA LLLSTATVDS MDWLPRNTNC GGILKEESGV IATYYGPKTN
60 70 80 90 100
CVWTIQMPPE YHVRVSIQYL QLNCNKESLE IIDGLPGSPV LGKICEGSLM
110 120 130
DYRSSGSIMT VKYIREPEHP ASFYEVLYFQ DPQA
Length:134
Mass (Da):15,036
Last modified:December 1, 1992 - v1
Checksum:i339BCFF8637D64C0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431T → H AA sequence (PubMed:1898381).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84603 mRNA. Translation: AAA30745.1.
BC110191 mRNA. Translation: AAI10192.1.
PIRiJQ1403.
RefSeqiNP_777041.1. NM_174616.3.
UniGeneiBt.457.

Genome annotation databases

GeneIDi282373.
KEGGibta:282373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84603 mRNA. Translation: AAA30745.1.
BC110191 mRNA. Translation: AAI10192.1.
PIRiJQ1403.
RefSeqiNP_777041.1. NM_174616.3.
UniGeneiBt.457.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SFPX-ray1.90A21-134[»]
ProteinModelPortaliP29392.
SMRiP29392. Positions 24-134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000014297.

Proteomic databases

PaxDbiP29392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282373.
KEGGibta:282373.

Organism-specific databases

CTDi282373.

Phylogenomic databases

eggNOGiENOG410JGFV. Eukaryota.
ENOG41118AQ. LUCA.
HOGENOMiHOG000168451.
HOVERGENiHBG004277.
InParanoidiP29392.

Miscellaneous databases

EvolutionaryTraceiP29392.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR000124. Spermadhesin.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00985. SPERMADHESIN_1. 1 hit.
PS00986. SPERMADHESIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization by cDNA cloning of the mRNA of a new growth factor from bovine seminal plasma: acidic seminal fluid protein."
    Wempe F., Einspanier R., Scheit K.H.
    Biochem. Biophys. Res. Commun. 183:232-237(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Seminal vesicle.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  3. "Characterization of a new bioactive protein from bovine seminal fluid."
    Einspanier R., Einspanier A., Wempe F., Scheit K.H.
    Biochem. Biophys. Res. Commun. 179:1006-1010(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-43.
    Tissue: Seminal vesicle.
  4. "Bovine seminal plasma aSFP: localization of disulfide bridges and detection of three different isoelectric forms."
    Einspanier R., Krause I., Calvete J.J., Toepfer-Petersen E., Klostermeyer H., Karg H.
    FEBS Lett. 344:61-64(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiSPAD1_BOVIN
AccessioniPrimary (citable) accession number: P29392
Secondary accession number(s): Q2YDJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.