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Protein

Guanine nucleotide-binding protein subunit beta-4

Gene

Gnb4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: MGI
  • substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiR-MMU-163359. Glucagon signaling in metabolic regulation.
R-MMU-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-MMU-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-MMU-420092. Glucagon-type ligand receptors.
R-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-4
Alternative name(s):
Transducin beta chain 4
Gene namesi
Name:Gnb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104581. Gnb4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 340339Guanine nucleotide-binding protein subunit beta-4PRO_0000127703Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei266 – 2661PhosphohistidineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP29387.
MaxQBiP29387.
PaxDbiP29387.
PeptideAtlasiP29387.
PRIDEiP29387.

2D gel databases

REPRODUCTION-2DPAGEP29387.

PTM databases

iPTMnetiP29387.
PhosphoSiteiP29387.
SwissPalmiP29387.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027669.
ExpressionAtlasiP29387. baseline and differential.
GenevisibleiP29387. MM.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-604N.
IntActiP29387. 3 interactions.
MINTiMINT-4095682.
STRINGi10090.ENSMUSP00000103869.

Structurei

3D structure databases

ProteinModelPortaliP29387.
SMRiP29387. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 9240WD 1Add
BLAST
Repeati95 – 13440WD 2Add
BLAST
Repeati141 – 17939WD 3Add
BLAST
Repeati182 – 22140WD 4Add
BLAST
Repeati224 – 26340WD 5Add
BLAST
Repeati268 – 30740WD 6Add
BLAST
Repeati310 – 33930WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP29387.
KOiK04538.
OMAiDYKTFVS.
OrthoDBiEOG091G0A7T.
PhylomeDBiP29387.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELEQLRQE AEQLRNQIQD ARKACNDATL VQITSNMDSV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGYDSRLL VSASQDGKLI IWDSYTTNKM HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELP GHTGYLSCCR
160 170 180 190 200
FLDDGQIITS SGDTTCALWD IETGQQTTTF TGHSGDVMSL SLSPDLKTFV
210 220 230 240 250
SGACDASSKL WDIRDGMCRQ SFTGHISDIN AVSFFPSGYA FATGSDDATC
260 270 280 290 300
RLFDLRADQE LLLYSHDNII CGITSVAFSK SGRLLLAGYD DFNCSVWDAL
310 320 330 340
KGGRSGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLRIWN
Length:340
Mass (Da):37,379
Last modified:January 23, 2007 - v4
Checksum:iF06827EBC2F0E966
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321N → D in AAB21609 (PubMed:1543505).Curated
Sequence conflicti132 – 1321N → D in AAA37756 (PubMed:1543505).Curated
Sequence conflicti132 – 1321N → D in AAA37664 (PubMed:1543505).Curated
Sequence conflicti140 – 1401P → A in AAB21609 (PubMed:1543505).Curated
Sequence conflicti140 – 1401P → A in AAA37756 (PubMed:1543505).Curated
Sequence conflicti140 – 1401P → A in AAA37664 (PubMed:1543505).Curated
Sequence conflicti238 – 2381G → V in AAM15922 (PubMed:11842130).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S86124 mRNA. Translation: AAB21609.1.
M87286 mRNA. Translation: AAA37756.1.
M63658 mRNA. Translation: AAA37664.1.
AF277893 mRNA. Translation: AAF82124.1.
AF501886 mRNA. Translation: AAM15922.1.
AK036816 mRNA. Translation: BAC29589.1.
AK152227 mRNA. Translation: BAE31054.1.
AK167416 mRNA. Translation: BAE39504.1.
BC028753 mRNA. Translation: AAH28753.1.
CCDSiCCDS17297.1.
PIRiJS0669. RGMSB4.
RefSeqiNP_038559.2. NM_013531.4.
XP_006535466.1. XM_006535403.2.
UniGeneiMm.139192.
Mm.393826.

Genome annotation databases

EnsembliENSMUST00000108234; ENSMUSP00000103869; ENSMUSG00000027669.
ENSMUST00000155737; ENSMUSP00000121127; ENSMUSG00000027669.
GeneIDi14696.
KEGGimmu:14696.
UCSCiuc008owl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S86124 mRNA. Translation: AAB21609.1.
M87286 mRNA. Translation: AAA37756.1.
M63658 mRNA. Translation: AAA37664.1.
AF277893 mRNA. Translation: AAF82124.1.
AF501886 mRNA. Translation: AAM15922.1.
AK036816 mRNA. Translation: BAC29589.1.
AK152227 mRNA. Translation: BAE31054.1.
AK167416 mRNA. Translation: BAE39504.1.
BC028753 mRNA. Translation: AAH28753.1.
CCDSiCCDS17297.1.
PIRiJS0669. RGMSB4.
RefSeqiNP_038559.2. NM_013531.4.
XP_006535466.1. XM_006535403.2.
UniGeneiMm.139192.
Mm.393826.

3D structure databases

ProteinModelPortaliP29387.
SMRiP29387. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-604N.
IntActiP29387. 3 interactions.
MINTiMINT-4095682.
STRINGi10090.ENSMUSP00000103869.

PTM databases

iPTMnetiP29387.
PhosphoSiteiP29387.
SwissPalmiP29387.

2D gel databases

REPRODUCTION-2DPAGEP29387.

Proteomic databases

EPDiP29387.
MaxQBiP29387.
PaxDbiP29387.
PeptideAtlasiP29387.
PRIDEiP29387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108234; ENSMUSP00000103869; ENSMUSG00000027669.
ENSMUST00000155737; ENSMUSP00000121127; ENSMUSG00000027669.
GeneIDi14696.
KEGGimmu:14696.
UCSCiuc008owl.1. mouse.

Organism-specific databases

CTDi59345.
MGIiMGI:104581. Gnb4.

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP29387.
KOiK04538.
OMAiDYKTFVS.
OrthoDBiEOG091G0A7T.
PhylomeDBiP29387.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiR-MMU-163359. Glucagon signaling in metabolic regulation.
R-MMU-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-MMU-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-MMU-420092. Glucagon-type ligand receptors.
R-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

PROiP29387.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027669.
ExpressionAtlasiP29387. baseline and differential.
GenevisibleiP29387. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBB4_MOUSE
AccessioniPrimary (citable) accession number: P29387
Secondary accession number(s): Q3TJJ1, Q8R475, Q9JHX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 143 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.