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Protein

Developmental protein SEPALLATA 1

Gene

SEP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor. Functions with SEPALLATA2/AGL4 and SEPALLATA3/AGL9 to ensure proper development of petals, stamens and carpels, and to prevent the indeterminate growth of the flower meristem. Forms a heterodimer via the K-box domain with AGAMOUS, that could be involved in genes regulation during floral meristem development.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental protein SEPALLATA 1
Alternative name(s):
Agamous-like MADS-box protein AGL2
Gene namesi
Name:SEP1
Synonyms:AGL2
Ordered Locus Names:At5g15800
ORF Names:F14F8_180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G15800.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Developmental protein SEPALLATA 1PRO_0000199483Add
BLAST

Proteomic databases

PaxDbiP29382.
PRIDEiP29382.

Expressioni

Tissue specificityi

Expressed mainly in carpels, and weakly in stamens.

Developmental stagei

Expressed early during flower development.

Gene expression databases

ExpressionAtlasiP29382. differential.
GenevisibleiP29382. AT.

Interactioni

Subunit structurei

Heterodimer with AGAMOUS capable of binding to CArG-box sequences. Interacts with AGL16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TT16Q8RYD93EBI-632935,EBI-621993

Protein-protein interaction databases

BioGridi16712. 22 interactions.
DIPiDIP-34935N.
IntActiP29382. 12 interactions.
STRINGi3702.AT5G15800.2.

Structurei

3D structure databases

ProteinModelPortaliP29382.
SMRiP29382. Positions 2-70, 90-174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5755MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini88 – 17891K-boxPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili85 – 17692Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiP29382.
KOiK09264.
PhylomeDBiP29382.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P29382-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN
60 70 80 90 100
RGKLYEFCSS SNMLKTLDRY QKCSYGSIEV NNKPAKELEN SYREYLKLKG
110 120 130 140 150
RYENLQRQQR NLLGEDLGPL NSKELEQLER QLDGSLKQVR SIKTQYMLDQ
160 170 180 190 200
LSDLQNKEQM LLETNRALAM KLDDMIGVRS HHMGGGGGWE GGEQNVTYAH
210 220 230 240 250
HQAQSQGLYQ PLECNPTLQM GYDNPVCSEQ ITATTQAQAQ QGNGYIPGWM

L
Length:251
Mass (Da):28,657
Last modified:December 6, 2002 - v2
Checksum:i867906D8D657621E
GO

Sequence cautioni

The sequence CAC01779.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1863Missing in AAA32732 (PubMed:1672119).Curated
Sequence conflicti241 – 2411Q → P in AAA32732 (PubMed:1672119).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55551 mRNA. Translation: AAA32732.1.
AL391144 Genomic DNA. Translation: CAC01779.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92207.1.
AK118608 mRNA. Translation: BAC43207.1.
BT006224 mRNA. Translation: AAP12873.1.
PIRiB39534.
T51409.
RefSeqiNP_001119230.1. NM_001125758.1.
NP_568322.1. NM_121585.3. [P29382-1]
UniGeneiAt.10163.
At.49025.

Genome annotation databases

EnsemblPlantsiAT5G15800.1; AT5G15800.1; AT5G15800. [P29382-1]
GeneIDi831436.
KEGGiath:AT5G15800.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55551 mRNA. Translation: AAA32732.1.
AL391144 Genomic DNA. Translation: CAC01779.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92207.1.
AK118608 mRNA. Translation: BAC43207.1.
BT006224 mRNA. Translation: AAP12873.1.
PIRiB39534.
T51409.
RefSeqiNP_001119230.1. NM_001125758.1.
NP_568322.1. NM_121585.3. [P29382-1]
UniGeneiAt.10163.
At.49025.

3D structure databases

ProteinModelPortaliP29382.
SMRiP29382. Positions 2-70, 90-174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16712. 22 interactions.
DIPiDIP-34935N.
IntActiP29382. 12 interactions.
STRINGi3702.AT5G15800.2.

Proteomic databases

PaxDbiP29382.
PRIDEiP29382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G15800.1; AT5G15800.1; AT5G15800. [P29382-1]
GeneIDi831436.
KEGGiath:AT5G15800.

Organism-specific databases

TAIRiAT5G15800.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiP29382.
KOiK09264.
PhylomeDBiP29382.

Miscellaneous databases

PROiP29382.

Gene expression databases

ExpressionAtlasiP29382. differential.
GenevisibleiP29382. AT.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes."
    Ma H., Yanofsky M.F., Meyerowitz E.M.
    Genes Dev. 5:484-495(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins."
    Fan H.-Y., Hu Y., Tudor M., Ma H.
    Plant J. 12:999-1010(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "B and C floral organ identity functions require SEPALLATA MADS-box genes."
    Pelaz S., Ditta G.S., Baumann E., Wisman E., Yanofsky M.F.
    Nature 405:200-203(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, DISRUPTION PHENOTYPE.
  8. Cited for: INTERACTION WITH AGL16.

Entry informationi

Entry nameiSEP1_ARATH
AccessioniPrimary (citable) accession number: P29382
Secondary accession number(s): Q9LFU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 6, 2002
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.