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Protein

Developmental protein SEPALLATA 1

Gene

SEP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcription factor. Functions with SEPALLATA2/AGL4 and SEPALLATA3/AGL9 to ensure proper development of petals, stamens and carpels, and to prevent the indeterminate growth of the flower meristem. Forms a heterodimer via the K-box domain with AGAMOUS, that could be involved in genes regulation during floral meristem development.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Flowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental protein SEPALLATA 1
Alternative name(s):
Agamous-like MADS-box protein AGL2
Gene namesi
Name:SEP1
Synonyms:AGL2
Ordered Locus Names:At5g15800
ORF Names:F14F8_180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G15800

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994831 – 251Developmental protein SEPALLATA 1Add BLAST251

Proteomic databases

PaxDbiP29382

Expressioni

Tissue specificityi

Expressed mainly in carpels, and weakly in stamens.

Developmental stagei

Expressed early during flower development.

Gene expression databases

ExpressionAtlasiP29382 baseline and differential
GenevisibleiP29382 AT

Interactioni

Subunit structurei

Heterodimer with AGAMOUS capable of binding to CArG-box sequences. Interacts with AGL16 (PubMed:15805477). Interacts with TT16/AGL32 (PubMed:16080001).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi16712, 22 interactors
DIPiDIP-34935N
IntActiP29382, 28 interactors
STRINGi3702.AT5G15800.2

Structurei

3D structure databases

ProteinModelPortaliP29382
SMRiP29382
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55
Domaini88 – 178K-boxPROSITE-ProRule annotationAdd BLAST91

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili85 – 176Sequence analysisAdd BLAST92

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014 Eukaryota
COG5068 LUCA
HOGENOMiHOG000155301
InParanoidiP29382
KOiK09264
PhylomeDBiP29382

Family and domain databases

CDDicd00265 MADS_MEF2_like, 1 hit
Gene3Di3.40.1810.10, 1 hit
InterProiView protein in InterPro
IPR033896 MADS_MEF2-like
IPR002487 TF_Kbox
IPR002100 TF_MADSbox
IPR036879 TF_MADSbox_sf
PfamiView protein in Pfam
PF01486 K-box, 1 hit
PF00319 SRF-TF, 1 hit
PRINTSiPR00404 MADSDOMAIN
SMARTiView protein in SMART
SM00432 MADS, 1 hit
SUPFAMiSSF55455 SSF55455, 1 hit
PROSITEiView protein in PROSITE
PS51297 K_BOX, 1 hit
PS00350 MADS_BOX_1, 1 hit
PS50066 MADS_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P29382-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN
60 70 80 90 100
RGKLYEFCSS SNMLKTLDRY QKCSYGSIEV NNKPAKELEN SYREYLKLKG
110 120 130 140 150
RYENLQRQQR NLLGEDLGPL NSKELEQLER QLDGSLKQVR SIKTQYMLDQ
160 170 180 190 200
LSDLQNKEQM LLETNRALAM KLDDMIGVRS HHMGGGGGWE GGEQNVTYAH
210 220 230 240 250
HQAQSQGLYQ PLECNPTLQM GYDNPVCSEQ ITATTQAQAQ QGNGYIPGWM

L
Length:251
Mass (Da):28,657
Last modified:December 6, 2002 - v2
Checksum:i867906D8D657621E
GO

Sequence cautioni

The sequence CAC01779 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184 – 186Missing in AAA32732 (PubMed:1672119).Curated3
Sequence conflicti241Q → P in AAA32732 (PubMed:1672119).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55551 mRNA Translation: AAA32732.1
AL391144 Genomic DNA Translation: CAC01779.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92207.1
AK118608 mRNA Translation: BAC43207.1
BT006224 mRNA Translation: AAP12873.1
PIRiB39534
T51409
RefSeqiNP_001119230.1, NM_001125758.2
NP_568322.1, NM_121585.4 [P29382-1]
UniGeneiAt.10163
At.49025

Genome annotation databases

EnsemblPlantsiAT5G15800.1; AT5G15800.1; AT5G15800 [P29382-1]
GeneIDi831436
GrameneiAT5G15800.1; AT5G15800.1; AT5G15800 [P29382-1]
KEGGiath:AT5G15800

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSEP1_ARATH
AccessioniPrimary (citable) accession number: P29382
Secondary accession number(s): Q9LFU6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 6, 2002
Last modified: April 25, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health