AT-rich interactive domain-containing protein 4A

Names and origin

Protein names

Recommended name:
    AT-rich interactive domain-containing protein 4A
Alternative name(s):
    ARID domain-containing protein 4A
    Retinoblastoma-binding protein 1
      Short name=RBBP-1

Gene names

Name:	ARID4A
Synonyms:	RBBP1, RBP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Interacts with the viral protein-binding domain of the retinoblastoma protein.
Subcellular location	Nucleus.
Sequence similarities	Contains 1 ARID domain.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: GDB transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	chromatin Inferred from electronic annotation. Source: InterPro transcriptional repressor complex Inferred from direct assay. Source: GDB
Molecular function	chromatin binding Inferred from electronic annotation. Source: InterPro protein binding Ref.4 Inferred from physical interaction. Source: GDB transcription factor activity Ref.1 Traceable author statement. Source: ProtInc transcription repressor activity Inferred from direct assay. Source: GDB
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform I (identifier: P29374-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform II (identifier: P29374-2) The sequence of this isoform differs from the canonical sequence as follows: 1121-1174: Missing.

Isoform III (identifier: P29374-3) The sequence of this isoform differs from the canonical sequence as follows: 1106-1174: Missing. 1175-1175: N → D

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1257

1257

AT-rich interactive domain-containing protein 4A

PRO_0000200580

Regions

Domain

309 – 401

93

ARID

Region

951 – 964

14

Retinoblastoma protein binding Potential

Amino acid modifications

Modified residue

676

1

Phosphoserine

Modified residue

1109

1

Phosphoserine Ref.5

Natural variations

Alternative sequence

1106 – 1174

69

Missing in isoform III.

VSP_004371

Alternative sequence

1121 – 1174

54

Missing in isoform II.

VSP_004373

Alternative sequence

1175

1

N → D in isoform III.

VSP_004372

Natural variant

412

1

H → P: dbSNP rs34982206.

VAR_031566

Natural variant

724

1

N → S: dbSNP rs2230098.

VAR_031567

Natural variant

779

1

T → A: dbSNP rs1051858. Ref.1

VAR_031568

Experimental info

Sequence conflict

385

1

V → L in AAB28543. Ref.1

Sequence conflict

618

1

R → S in AAB28543. Ref.1

Sequence conflict

653

1

K → V in AAB25833. Ref.3

Sequence conflict

653

1

K → V in AAB25834. Ref.3

Sequence conflict

653

1

K → V in AAB25835. Ref.3

Sequence conflict

1178

1

D → S Ref.4

Sequence conflict

1196 – 1201

6

IRKYYM → SENIICL Ref.4

Secondary structure

1

.

1257

Helix Strand Turn

Details...

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Sequences

Sequence		Length	Mass (Da)
Isoform I [UniParc]. Last modified September 23, 2008. Version 3. Checksum: 5B3F6A5AFB7588CF Show »	FASTA	1,257	142,752
10 20 30 40 50 60 MKAADEPAYL TVGTDVSAKY RGAFCEAKIK TVKRLVKVKV LLKQDNTTQL VQDDQVKGPL 70 80 90 100 110 120 RVGAIVETRT SDGSFQEAII SKLTDASWYT VVFDDGDERT LRRTSLCLKG ERHFAESETL 130 140 150 160 170 180 DQLPLTNPEH FGTPVIAKKT NRGRRSSLPV TEDEKEEESS EEEDEDKRRL NDELLGKVVS 190 200 210 220 230 240 VVSATERTEW YPALVISPSC NDDITVKKDQ CLVRSFIDSK FYSIARKDIK EVDILNLPES 250 260 270 280 290 300 ELSTKPGLQK ASIFLKTRVV PDNWKMDISE ILESSSSDDE DGPAEENDEE KEKEAKKTEE 310 320 330 340 350 360 EVPEEELDPE ERDNFLQQLY KFMEDRGTPI NKPPVLGYKD LNLFKLFRLV YHQGGCDNID 370 380 390 400 410 420 SGAVWKQIYM DLGIPILNSA ASYNVKTAYR KYLYGFEEYC RSANIQFRTV HHHEPKVKEE 430 440 450 460 470 480 KKDLEESMEE ALKLDQEMPL TEVKSEPEEN IDSNSESERE EIELKSPRGR RRIARDVNSI 490 500 510 520 530 540 KKEIEEEKTE DKLKDNDTEN KDVDDDYETA EKKENELLLG RKNTPKQKEK KIKKQEDSDK 550 560 570 580 590 600 DSDEEEEKSQ EREETESKCD SEGEEDEEDM EPCLTGTKVK VKYGRGKTQK IYEASIKSTE 610 620 630 640 650 660 IDDGEVLYLV HYYGWNVRYD EWVKADRIIW PLDKGGPKKK QKKKAKNKED SEKDEKRDEE 670 680 690 700 710 720 RQKSKRGRPP LKSTLSSNMP YGLSKTANSE GKSDSCSSDS ETEDALEKNL INEELSLKDE 730 740 750 760 770 780 LEKNENLNDD KLDEENPKIS AHILKENDRT QMQPLETLKL EVGENEQIVQ IFGNKMEKTE 790 800 810 820 830 840 EVKKEAEKSP KGKGRRSKTK DLSLEIIKIS SFGQNEAGSE PHIEAHSLEL SSLDNKNFSS 850 860 870 880 890 900 ATEDEIDQCV KEKKLKRKIL GQSSPEKKIR IENGMEMTNT VSQERTSDCI GSEGMKNLNF 910 920 930 940 950 960 EQHFERENEG MPSLIAESNQ CIQQLTSERF DSPAEETVNI PLKEDEDAMP LIGPETLVCH 970 980 990 1000 1010 1020 EVDLDDLDEK DKTSIEDVAV ESSESNSLVS IPPALPPVVQ HNFSVASPLT LSQDESRSVK 1030 1040 1050 1060 1070 1080 SESDITIEVD SIAEESQEGL CERESANGFE TNVASGTCSI IVQERESREK GQKRPSDGNS 1090 1100 1110 1120 1130 1140 GLMAKKQKRT PKRTSAAAKN EKNGTGQSSD SEDLPVLDNS SKCTPVKHLN VSKPQKLARS 1150 1160 1170 1180 1190 1200 PARISPHIKD GEKDKHREKH PNSSPRTYKW SFQLNELDNM NSTERISFLQ EKLQEIRKYY 1210 1220 1230 1240 1250 MSLKSEVATI DRRRKRLKKK DREVSHAGAS MSSASSDTGM SPSSSSPPQN VLAVECR « Hide
Isoform II. Checksum: B2A06AA7071AAAE2 Show »	FASTA	1,203	136,495
10 20 30 40 50 60 MKAADEPAYL TVGTDVSAKY RGAFCEAKIK TVKRLVKVKV LLKQDNTTQL VQDDQVKGPL 70 80 90 100 110 120 RVGAIVETRT SDGSFQEAII SKLTDASWYT VVFDDGDERT LRRTSLCLKG ERHFAESETL 130 140 150 160 170 180 DQLPLTNPEH FGTPVIAKKT NRGRRSSLPV TEDEKEEESS EEEDEDKRRL NDELLGKVVS 190 200 210 220 230 240 VVSATERTEW YPALVISPSC NDDITVKKDQ CLVRSFIDSK FYSIARKDIK EVDILNLPES 250 260 270 280 290 300 ELSTKPGLQK ASIFLKTRVV PDNWKMDISE ILESSSSDDE DGPAEENDEE KEKEAKKTEE 310 320 330 340 350 360 EVPEEELDPE ERDNFLQQLY KFMEDRGTPI NKPPVLGYKD LNLFKLFRLV YHQGGCDNID 370 380 390 400 410 420 SGAVWKQIYM DLGIPILNSA ASYNVKTAYR KYLYGFEEYC RSANIQFRTV HHHEPKVKEE 430 440 450 460 470 480 KKDLEESMEE ALKLDQEMPL TEVKSEPEEN IDSNSESERE EIELKSPRGR RRIARDVNSI 490 500 510 520 530 540 KKEIEEEKTE DKLKDNDTEN KDVDDDYETA EKKENELLLG RKNTPKQKEK KIKKQEDSDK 550 560 570 580 590 600 DSDEEEEKSQ EREETESKCD SEGEEDEEDM EPCLTGTKVK VKYGRGKTQK IYEASIKSTE 610 620 630 640 650 660 IDDGEVLYLV HYYGWNVRYD EWVKADRIIW PLDKGGPKKK QKKKAKNKED SEKDEKRDEE 670 680 690 700 710 720 RQKSKRGRPP LKSTLSSNMP YGLSKTANSE GKSDSCSSDS ETEDALEKNL INEELSLKDE 730 740 750 760 770 780 LEKNENLNDD KLDEENPKIS AHILKENDRT QMQPLETLKL EVGENEQIVQ IFGNKMEKTE 790 800 810 820 830 840 EVKKEAEKSP KGKGRRSKTK DLSLEIIKIS SFGQNEAGSE PHIEAHSLEL SSLDNKNFSS 850 860 870 880 890 900 ATEDEIDQCV KEKKLKRKIL GQSSPEKKIR IENGMEMTNT VSQERTSDCI GSEGMKNLNF 910 920 930 940 950 960 EQHFERENEG MPSLIAESNQ CIQQLTSERF DSPAEETVNI PLKEDEDAMP LIGPETLVCH 970 980 990 1000 1010 1020 EVDLDDLDEK DKTSIEDVAV ESSESNSLVS IPPALPPVVQ HNFSVASPLT LSQDESRSVK 1030 1040 1050 1060 1070 1080 SESDITIEVD SIAEESQEGL CERESANGFE TNVASGTCSI IVQERESREK GQKRPSDGNS 1090 1100 1110 1120 1130 1140 GLMAKKQKRT PKRTSAAAKN EKNGTGQSSD SEDLPVLDNS NELDNMNSTE RISFLQEKLQ 1150 1160 1170 1180 1190 1200 EIRKYYMSLK SEVATIDRRR KRLKKKDREV SHAGASMSSA SSDTGMSPSS SSPPQNVLAV ECR « Hide
Isoform III. Checksum: 0576FA849F761B54 Show »	FASTA	1,188	134,951
10 20 30 40 50 60 MKAADEPAYL TVGTDVSAKY RGAFCEAKIK TVKRLVKVKV LLKQDNTTQL VQDDQVKGPL 70 80 90 100 110 120 RVGAIVETRT SDGSFQEAII SKLTDASWYT VVFDDGDERT LRRTSLCLKG ERHFAESETL 130 140 150 160 170 180 DQLPLTNPEH FGTPVIAKKT NRGRRSSLPV TEDEKEEESS EEEDEDKRRL NDELLGKVVS 190 200 210 220 230 240 VVSATERTEW YPALVISPSC NDDITVKKDQ CLVRSFIDSK FYSIARKDIK EVDILNLPES 250 260 270 280 290 300 ELSTKPGLQK ASIFLKTRVV PDNWKMDISE ILESSSSDDE DGPAEENDEE KEKEAKKTEE 310 320 330 340 350 360 EVPEEELDPE ERDNFLQQLY KFMEDRGTPI NKPPVLGYKD LNLFKLFRLV YHQGGCDNID 370 380 390 400 410 420 SGAVWKQIYM DLGIPILNSA ASYNVKTAYR KYLYGFEEYC RSANIQFRTV HHHEPKVKEE 430 440 450 460 470 480 KKDLEESMEE ALKLDQEMPL TEVKSEPEEN IDSNSESERE EIELKSPRGR RRIARDVNSI 490 500 510 520 530 540 KKEIEEEKTE DKLKDNDTEN KDVDDDYETA EKKENELLLG RKNTPKQKEK KIKKQEDSDK 550 560 570 580 590 600 DSDEEEEKSQ EREETESKCD SEGEEDEEDM EPCLTGTKVK VKYGRGKTQK IYEASIKSTE 610 620 630 640 650 660 IDDGEVLYLV HYYGWNVRYD EWVKADRIIW PLDKGGPKKK QKKKAKNKED SEKDEKRDEE 670 680 690 700 710 720 RQKSKRGRPP LKSTLSSNMP YGLSKTANSE GKSDSCSSDS ETEDALEKNL INEELSLKDE 730 740 750 760 770 780 LEKNENLNDD KLDEENPKIS AHILKENDRT QMQPLETLKL EVGENEQIVQ IFGNKMEKTE 790 800 810 820 830 840 EVKKEAEKSP KGKGRRSKTK DLSLEIIKIS SFGQNEAGSE PHIEAHSLEL SSLDNKNFSS 850 860 870 880 890 900 ATEDEIDQCV KEKKLKRKIL GQSSPEKKIR IENGMEMTNT VSQERTSDCI GSEGMKNLNF 910 920 930 940 950 960 EQHFERENEG MPSLIAESNQ CIQQLTSERF DSPAEETVNI PLKEDEDAMP LIGPETLVCH 970 980 990 1000 1010 1020 EVDLDDLDEK DKTSIEDVAV ESSESNSLVS IPPALPPVVQ HNFSVASPLT LSQDESRSVK 1030 1040 1050 1060 1070 1080 SESDITIEVD SIAEESQEGL CERESANGFE TNVASGTCSI IVQERESREK GQKRPSDGNS 1090 1100 1110 1120 1130 1140 GLMAKKQKRT PKRTSAAAKN EKNGTDELDN MNSTERISFL QEKLQEIRKY YMSLKSEVAT 1150 1160 1170 1180 IDRRRKRLKK KDREVSHAGA SMSSASSDTG MSPSSSSPPQ NVLAVECR « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the retinoblastoma binding proteins RBP1 and RBP2." Fattaey A.R., Helin K., Dembski M.S., Dyson N., Harlow E., Vuocolo G.A., Hanobik M.G., Haskell K.M., Oliff A., Defeo-Jones D., Jones R.E. Oncogene 8:3149-3156(1993) [PubMed: 8414517] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM I), VARIANT ALA-779.
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Alternative splicing of the RBP1 gene clusters in an internal exon that encodes potential phosphorylation sites." Otterson G.A., Kratzke R.A., Lin A.Y., Johnston P.G., Kaye F.J. Oncogene 8:949-957(1993) [PubMed: 8455946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 338-1257 (ISOFORMS I; II AND III), SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
[4]	"Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product." Defeo-Jones D., Huang P.S., Jones R.E., Haskell K.M., Vuocolo G.A., Hanobik M.G., Huber H.E., Oliff A. Nature 352:251-254(1991) [PubMed: 1857421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 855-1203 (ISOFORM I).
[5]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1109, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-676 AND SER-1109, MASS SPECTROMETRY. Tissue: T-cell.
[7]	"Solution structure of the RBB1NT domain of human RB(Retinoblastoma)-binding protein 1." RIKEN structural genomics initiative (RSGI) Submitted (APR-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 170-277.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

S66427 mRNA. Translation: AAB28543.1.
AL132989 Genomic DNA. No translation available.
AL139021 Genomic DNA. No translation available.
S57153 mRNA. Translation: AAB25833.1.
S57160 mRNA. Translation: AAB25834.1.
S57162 mRNA. Translation: AAB25835.2.

IPI

IPI00218648.
IPI00296069.
IPI00328418.

PIR

I58383.

RefSeq

NP_002883.3.
NP_075376.2.
NP_075377.2.

UniGene

Hs.161000

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YRV	NMR	-	A	170-274	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:463N.

IntAct

P29374. 1 interaction.

STRING

P29374.

PTM databases

PhosphoSite

P29374.

Proteomic databases

PRIDE

P29374.

Genome annotation databases

Ensembl

ENST00000355431; ENSP00000347602; ENSG00000032219; Homo sapiens. [Genome view]

GeneID

5926.

KEGG

hsa:5926.

UCSC

uc001xdo.1. human.
uc001xdp.1. human.

Organism-specific databases

CTD

5926.

GeneCards

GC14P057835.

H-InvDB

HIX0037655.

HGNC

HGNC:9885. ARID4A.

MIM

180201. gene.

PharmGKB

PA34249.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P29374.

HOVERGEN

P29374.

OMA

RQKSKRG

OrthoDB

EOG91ZHWT

Gene expression databases

ArrayExpress

P29374.

Bgee

P29374.

CleanEx

HS_ARID4A.
HS_RBP1.

Genevestigator

P29374.

GermOnline

ENSG00000032219. Homo sapiens.

Family and domain databases

InterPro

IPR001606. ARID.
IPR000953. Chromodomain.
IPR012603. RBB1NT.
IPR002999. Tudor.
[Graphical view]

Gene3D

G3DSA:1.10.150.60. ARID. 1 hit.

Pfam

PF01388. ARID. 1 hit.
PF08169. RBB1NT. 1 hit.
[Graphical view]

SMART

SM00501. BRIGHT. 1 hit.
SM00298. CHROMO. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]

PROSITE

PS51011. ARID. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

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Entry information

Entry name

ARI4A_HUMAN

Accession

Primary (citable) accession number: P29374
Secondary accession number(s): Q15991, Q15992, Q15993

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	September 23, 2008
Last modified:	November 24, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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