Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-3-methyladenine glycosylase

Gene

MPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Enzyme regulationi

Binding to SSBP1 in mitochondria inhibits glycosylase activity in the context of a single-stranded DNA (ssDNA), but not a double-stranded DNA (dsDNA) substrates.1 Publication

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: ProtInc
  • depurination Source: Reactome
  • DNA dealkylation involved in DNA repair Source: ProtInc

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BRENDAi3.2.2.21 2681
ReactomeiR-HSA-110330 Recognition and association of DNA glycosylase with site containing an affected purine
R-HSA-110331 Cleavage of the damaged purine
R-HSA-110357 Displacement of DNA glycosylase by APEX1
SABIO-RKP29372

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-alkyladenine DNA glycosylase
3-methyladenine DNA glycosidase
ADPG
N-methylpurine-DNA glycosylase
Gene namesi
Name:MPG
Synonyms:AAG, ANPG, MID1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103152.11
HGNCiHGNC:7211 MPG
MIMi156565 gene
neXtProtiNX_P29372

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4350
OpenTargetsiENSG00000103152
PharmGKBiPA30917

Chemistry databases

ChEMBLiCHEMBL3396943

Polymorphism and mutation databases

BioMutaiMPG

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
ChainiPRO_000010006518 – 298DNA-3-methyladenine glycosylaseAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP29372
PaxDbiP29372
PeptideAtlasiP29372
PRIDEiP29372
TopDownProteomicsiP29372-1 [P29372-1]

PTM databases

iPTMnetiP29372
PhosphoSitePlusiP29372

Expressioni

Gene expression databases

BgeeiENSG00000103152
CleanExiHS_MID1
HS_MPG
ExpressionAtlasiP29372 baseline and differential
GenevisibleiP29372 HS

Organism-specific databases

HPAiHPA006531

Interactioni

Subunit structurei

Binds MBD1. Binds SSBP1.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi110490, 70 interactors
IntActiP29372, 15 interactors
MINTiP29372
STRINGi9606.ENSP00000219431

Chemistry databases

BindingDBiP29372

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi82 – 84Combined sources3
Helixi88 – 91Combined sources4
Helixi95 – 101Combined sources7
Turni102 – 104Combined sources3
Beta strandi106 – 110Combined sources5
Beta strandi116 – 127Combined sources12
Beta strandi129 – 131Combined sources3
Helixi138 – 140Combined sources3
Helixi145 – 150Combined sources6
Beta strandi155 – 161Combined sources7
Turni162 – 164Combined sources3
Beta strandi165 – 171Combined sources7
Beta strandi178 – 188Combined sources11
Helixi190 – 199Combined sources10
Helixi211 – 213Combined sources3
Beta strandi214 – 217Combined sources4
Helixi218 – 224Combined sources7
Helixi229 – 231Combined sources3
Turni236 – 238Combined sources3
Beta strandi240 – 245Combined sources6
Helixi253 – 255Combined sources3
Beta strandi256 – 259Combined sources4
Turni268 – 272Combined sources5
Beta strandi276 – 279Combined sources4
Turni290 – 292Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BNKX-ray2.70A80-295[»]
1EWNX-ray2.10A80-298[»]
1F4RX-ray2.40A80-298[»]
1F6OX-ray2.40A80-298[»]
3QI5X-ray2.20A/B84-298[»]
3UBYX-ray2.00A/B84-298[»]
ProteinModelPortaliP29372
SMRiP29372
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29372

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA glycosylase MPG family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4486 Eukaryota
COG2094 LUCA
GeneTreeiENSGT00390000009825
HOGENOMiHOG000224224
HOVERGENiHBG000019
InParanoidiP29372
KOiK03652
OMAiVEAYHHT
OrthoDBiEOG091G0NE4
PhylomeDBiP29372
TreeFamiTF331768

Family and domain databases

CDDicd00540 AAG, 1 hit
Gene3Di3.10.300.10, 1 hit
HAMAPiMF_00527 3MGH, 1 hit
InterProiView protein in InterPro
IPR011034 Formyl_transferase-like_C_sf
IPR003180 MPG
IPR036995 MPG_sf
PANTHERiPTHR10429 PTHR10429, 1 hit
PfamiView protein in Pfam
PF02245 Pur_DNA_glyco, 1 hit
SUPFAMiSSF50486 SSF50486, 1 hit
TIGRFAMsiTIGR00567 3mg, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P29372-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVTPALQMKK PKQFCRRMGQ KKQRPARAGQ PHSSSDAAQA PAEQPHSSSD
60 70 80 90 100
AAQAPCPRER CLGPPTTPGP YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR
110 120 130 140 150
AFLGQVLVRR LPNGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF
160 170 180 190 200
MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGL ETMRQLRSTL
210 220 230 240 250
RKGTASRVLK DRELCSGPSK LCQALAINKS FDQRDLAQDE AVWLERGPLE
260 270 280 290
PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDTQA
Length:298
Mass (Da):32,869
Last modified:September 23, 2008 - v3
Checksum:iBEA8C4CB250D572B
GO
Isoform 2 (identifier: P29372-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MVTPALQMKKPK → MPARSGA
     195-196: QL → HV

Show »
Length:293
Mass (Da):32,181
Checksum:i81BE31859298D43C
GO
Isoform 3 (identifier: P29372-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MVTPALQMKKPK → MPARSGA

Show »
Length:293
Mass (Da):32,186
Checksum:i6F710D76409B2D81
GO
Isoform 4 (identifier: P29372-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Gene prediction based on EST data.
Show »
Length:281
Mass (Da):30,824
Checksum:i6826A6C692AABA9C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13Q → QV in AAK61213 (PubMed:11157797).Curated1
Sequence conflicti29 – 31GQP → ARA in AAB19537 (PubMed:1874728).Curated3
Sequence conflicti44Q → R in AAB19537 (PubMed:1874728).Curated1
Sequence conflicti69 – 71GPY → SKD in AAA58369 (PubMed:1645538).Curated3
Sequence conflicti69 – 71GPY → SKD in CAA39875 (PubMed:1645538).Curated3
Sequence conflicti82H → L in AAA58369 (PubMed:1645538).Curated1
Sequence conflicti82H → L in CAA39875 (PubMed:1645538).Curated1
Sequence conflicti134A → P in AAA58627 (PubMed:1924375).Curated1
Sequence conflicti287V → E in AAA58369 (PubMed:1645538).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01913822K → Q1 PublicationCorresponds to variant dbSNP:rs3176383Ensembl.1
Natural variantiVAR_01483164P → L. Corresponds to variant dbSNP:rs2308315Ensembl.1
Natural variantiVAR_01483271Y → H1 PublicationCorresponds to variant dbSNP:rs2266607Ensembl.1
Natural variantiVAR_05009693Q → R. Corresponds to variant dbSNP:rs25671Ensembl.1
Natural variantiVAR_014833120R → C. Corresponds to variant dbSNP:rs2308313Ensembl.1
Natural variantiVAR_014834141R → Q. Corresponds to variant dbSNP:rs2308312Ensembl.1
Natural variantiVAR_014835258A → V1 PublicationCorresponds to variant dbSNP:rs769193Ensembl.1
Natural variantiVAR_014836298A → S1 PublicationCorresponds to variant dbSNP:rs2234949Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0466781 – 17Missing in isoform 4. CuratedAdd BLAST17
Alternative sequenceiVSP_0032491 – 12MVTPA…MKKPK → MPARSGA in isoform 2 and isoform 3. 3 PublicationsAdd BLAST12
Alternative sequenceiVSP_035485195 – 196QL → HV in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74905 mRNA Translation: AAA58627.1
L10752 mRNA Translation: AAF77073.1
AY258284 mRNA Translation: AAP82229.1
AY305873 mRNA Translation: AAQ95215.1
AF499437 Genomic DNA Translation: AAM14628.1
AE006462 Genomic DNA Translation: AAK61213.1
Z69720 Genomic DNA Translation: CAA93540.1
Z69720 Genomic DNA Translation: CAI95610.1
CH471112 Genomic DNA Translation: EAW85871.1
BC014991 mRNA Translation: AAH14991.1
S51033 mRNA Translation: AAB19537.1
X56528 mRNA Translation: CAA39875.1
M71215 mRNA Translation: AAA58369.1
M99626 mRNA Translation: AAB46421.1
CCDSiCCDS32345.1 [P29372-4]
CCDS32346.1 [P29372-1]
CCDS42087.1 [P29372-5]
PIRiA40798
A41230
A47471
JN0062
RefSeqiNP_001015052.1, NM_001015052.2 [P29372-4]
NP_001015054.1, NM_001015054.2 [P29372-5]
NP_002425.2, NM_002434.3 [P29372-1]
UniGeneiHs.459596

Genome annotation databases

EnsembliENST00000219431; ENSP00000219431; ENSG00000103152 [P29372-1]
ENST00000356432; ENSP00000348809; ENSG00000103152 [P29372-4]
ENST00000397817; ENSP00000380918; ENSG00000103152 [P29372-5]
GeneIDi4350
KEGGihsa:4350
UCSCiuc002cfm.4 human [P29372-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry namei3MG_HUMAN
AccessioniPrimary (citable) accession number: P29372
Secondary accession number(s): G5E9E2
, Q13770, Q15275, Q15961, Q5J9I4, Q96BZ6, Q96S33, Q9NNX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 23, 2008
Last modified: May 23, 2018
This is version 196 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health