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Protein

DNA-3-methyladenine glycosylase

Gene

MPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Enzyme regulationi

Binding to SSBP1 in mitochondria inhibits glycosylase activity in the context of a single-stranded DNA (ssDNA), but not a double-stranded DNA (dsDNA) substrates.1 Publication

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: ProtInc
  • depurination Source: Reactome
  • DNA dealkylation involved in DNA repair Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciZFISH:HS02461-MONOMER.
BRENDAi3.2.2.21. 2681.
ReactomeiR-HSA-110330. Recognition and association of DNA glycosylase with site containing an affected purine.
R-HSA-110331. Cleavage of the damaged purine.
R-HSA-110357. Displacement of DNA glycosylase by APEX1.
SABIO-RKP29372.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-alkyladenine DNA glycosylase
3-methyladenine DNA glycosidase
ADPG
N-methylpurine-DNA glycosylase
Gene namesi
Name:MPG
Synonyms:AAG, ANPG, MID1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:7211. MPG.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Mitochondrion matrixmitochondrion nucleoid 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4350.
OpenTargetsiENSG00000103152.
PharmGKBiPA30917.

Chemistry databases

ChEMBLiCHEMBL3396943.

Polymorphism and mutation databases

BioMutaiMPG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
ChainiPRO_000010006518 – 298DNA-3-methyladenine glycosylaseAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP29372.
PaxDbiP29372.
PeptideAtlasiP29372.
PRIDEiP29372.
TopDownProteomicsiP29372-1. [P29372-1]

PTM databases

iPTMnetiP29372.
PhosphoSitePlusiP29372.

Expressioni

Gene expression databases

BgeeiENSG00000103152.
CleanExiHS_MID1.
HS_MPG.
ExpressionAtlasiP29372. baseline and differential.
GenevisibleiP29372. HS.

Organism-specific databases

HPAiHPA006531.

Interactioni

Subunit structurei

Binds MBD1. Binds SSBP1.

Binary interactionsi

WithEntry#Exp.IntActNotes
HSD17B14Q9BPX13EBI-1043398,EBI-742664
PRNPP041564EBI-1043398,EBI-977302

Protein-protein interaction databases

BioGridi110490. 70 interactors.
IntActiP29372. 15 interactors.
MINTiMINT-135511.
STRINGi9606.ENSP00000219431.

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi82 – 84Combined sources3
Helixi88 – 91Combined sources4
Helixi95 – 101Combined sources7
Turni102 – 104Combined sources3
Beta strandi106 – 110Combined sources5
Beta strandi116 – 127Combined sources12
Beta strandi129 – 131Combined sources3
Helixi138 – 140Combined sources3
Helixi145 – 150Combined sources6
Beta strandi155 – 161Combined sources7
Turni162 – 164Combined sources3
Beta strandi165 – 171Combined sources7
Beta strandi178 – 188Combined sources11
Helixi190 – 199Combined sources10
Helixi211 – 213Combined sources3
Beta strandi214 – 217Combined sources4
Helixi218 – 224Combined sources7
Helixi229 – 231Combined sources3
Turni236 – 238Combined sources3
Beta strandi240 – 245Combined sources6
Helixi253 – 255Combined sources3
Beta strandi256 – 259Combined sources4
Turni268 – 272Combined sources5
Beta strandi276 – 279Combined sources4
Turni290 – 292Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BNKX-ray2.70A80-295[»]
1EWNX-ray2.10A80-298[»]
1F4RX-ray2.40A80-298[»]
1F6OX-ray2.40A80-298[»]
3QI5X-ray2.20A/B84-298[»]
3UBYX-ray2.00A/B84-298[»]
ProteinModelPortaliP29372.
SMRiP29372.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29372.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA glycosylase MPG family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4486. Eukaryota.
COG2094. LUCA.
GeneTreeiENSGT00390000009825.
HOGENOMiHOG000224224.
HOVERGENiHBG000019.
InParanoidiP29372.
KOiK03652.
OMAiTHYPLRF.
OrthoDBiEOG091G0NE4.
PhylomeDBiP29372.
TreeFamiTF331768.

Family and domain databases

CDDicd00540. AAG. 1 hit.
Gene3Di3.10.300.10. 1 hit.
HAMAPiMF_00527. 3MGH. 1 hit.
InterProiIPR011034. Formyl_transferase_C-like.
IPR003180. PurDNA_glycsylse.
[Graphical view]
PANTHERiPTHR10429. PTHR10429. 1 hit.
PfamiPF02245. Pur_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
TIGRFAMsiTIGR00567. 3mg. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P29372-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVTPALQMKK PKQFCRRMGQ KKQRPARAGQ PHSSSDAAQA PAEQPHSSSD
60 70 80 90 100
AAQAPCPRER CLGPPTTPGP YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR
110 120 130 140 150
AFLGQVLVRR LPNGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF
160 170 180 190 200
MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGL ETMRQLRSTL
210 220 230 240 250
RKGTASRVLK DRELCSGPSK LCQALAINKS FDQRDLAQDE AVWLERGPLE
260 270 280 290
PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDTQA
Length:298
Mass (Da):32,869
Last modified:September 23, 2008 - v3
Checksum:iBEA8C4CB250D572B
GO
Isoform 2 (identifier: P29372-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MVTPALQMKKPK → MPARSGA
     195-196: QL → HV

Show »
Length:293
Mass (Da):32,181
Checksum:i81BE31859298D43C
GO
Isoform 3 (identifier: P29372-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MVTPALQMKKPK → MPARSGA

Show »
Length:293
Mass (Da):32,186
Checksum:i6F710D76409B2D81
GO
Isoform 4 (identifier: P29372-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Gene prediction based on EST data.
Show »
Length:281
Mass (Da):30,824
Checksum:i6826A6C692AABA9C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13Q → QV in AAK61213 (PubMed:11157797).Curated1
Sequence conflicti29 – 31GQP → ARA in AAB19537 (PubMed:1874728).Curated3
Sequence conflicti44Q → R in AAB19537 (PubMed:1874728).Curated1
Sequence conflicti69 – 71GPY → SKD in AAA58369 (PubMed:1645538).Curated3
Sequence conflicti69 – 71GPY → SKD in CAA39875 (PubMed:1645538).Curated3
Sequence conflicti82H → L in AAA58369 (PubMed:1645538).Curated1
Sequence conflicti82H → L in CAA39875 (PubMed:1645538).Curated1
Sequence conflicti134A → P in AAA58627 (PubMed:1924375).Curated1
Sequence conflicti287V → E in AAA58369 (PubMed:1645538).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01913822K → Q.1 PublicationCorresponds to variant rs3176383dbSNPEnsembl.1
Natural variantiVAR_01483164P → L.Corresponds to variant rs2308315dbSNPEnsembl.1
Natural variantiVAR_01483271Y → H.1 PublicationCorresponds to variant rs2266607dbSNPEnsembl.1
Natural variantiVAR_05009693Q → R.Corresponds to variant rs25671dbSNPEnsembl.1
Natural variantiVAR_014833120R → C.Corresponds to variant rs2308313dbSNPEnsembl.1
Natural variantiVAR_014834141R → Q.Corresponds to variant rs2308312dbSNPEnsembl.1
Natural variantiVAR_014835258A → V.1 PublicationCorresponds to variant rs769193dbSNPEnsembl.1
Natural variantiVAR_014836298A → S.1 PublicationCorresponds to variant rs2234949dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0466781 – 17Missing in isoform 4. CuratedAdd BLAST17
Alternative sequenceiVSP_0032491 – 12MVTPA…MKKPK → MPARSGA in isoform 2 and isoform 3. 3 PublicationsAdd BLAST12
Alternative sequenceiVSP_035485195 – 196QL → HV in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74905 mRNA. Translation: AAA58627.1.
L10752 mRNA. Translation: AAF77073.1.
AY258284 mRNA. Translation: AAP82229.1.
AY305873 mRNA. Translation: AAQ95215.1.
AF499437 Genomic DNA. Translation: AAM14628.1.
AE006462 Genomic DNA. Translation: AAK61213.1.
Z69720 Genomic DNA. Translation: CAA93540.1.
Z69720 Genomic DNA. Translation: CAI95610.1.
CH471112 Genomic DNA. Translation: EAW85871.1.
BC014991 mRNA. Translation: AAH14991.1.
S51033 mRNA. Translation: AAB19537.1.
X56528 mRNA. Translation: CAA39875.1.
M71215 mRNA. Translation: AAA58369.1.
M99626 mRNA. Translation: AAB46421.1.
CCDSiCCDS32345.1. [P29372-4]
CCDS32346.1. [P29372-1]
CCDS42087.1. [P29372-5]
PIRiA40798.
A41230.
A47471.
JN0062.
RefSeqiNP_001015052.1. NM_001015052.2. [P29372-4]
NP_001015054.1. NM_001015054.2. [P29372-5]
NP_002425.2. NM_002434.3. [P29372-1]
UniGeneiHs.459596.

Genome annotation databases

EnsembliENST00000219431; ENSP00000219431; ENSG00000103152. [P29372-1]
ENST00000356432; ENSP00000348809; ENSG00000103152. [P29372-4]
ENST00000397817; ENSP00000380918; ENSG00000103152. [P29372-5]
GeneIDi4350.
KEGGihsa:4350.
UCSCiuc002cfm.4. human. [P29372-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74905 mRNA. Translation: AAA58627.1.
L10752 mRNA. Translation: AAF77073.1.
AY258284 mRNA. Translation: AAP82229.1.
AY305873 mRNA. Translation: AAQ95215.1.
AF499437 Genomic DNA. Translation: AAM14628.1.
AE006462 Genomic DNA. Translation: AAK61213.1.
Z69720 Genomic DNA. Translation: CAA93540.1.
Z69720 Genomic DNA. Translation: CAI95610.1.
CH471112 Genomic DNA. Translation: EAW85871.1.
BC014991 mRNA. Translation: AAH14991.1.
S51033 mRNA. Translation: AAB19537.1.
X56528 mRNA. Translation: CAA39875.1.
M71215 mRNA. Translation: AAA58369.1.
M99626 mRNA. Translation: AAB46421.1.
CCDSiCCDS32345.1. [P29372-4]
CCDS32346.1. [P29372-1]
CCDS42087.1. [P29372-5]
PIRiA40798.
A41230.
A47471.
JN0062.
RefSeqiNP_001015052.1. NM_001015052.2. [P29372-4]
NP_001015054.1. NM_001015054.2. [P29372-5]
NP_002425.2. NM_002434.3. [P29372-1]
UniGeneiHs.459596.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BNKX-ray2.70A80-295[»]
1EWNX-ray2.10A80-298[»]
1F4RX-ray2.40A80-298[»]
1F6OX-ray2.40A80-298[»]
3QI5X-ray2.20A/B84-298[»]
3UBYX-ray2.00A/B84-298[»]
ProteinModelPortaliP29372.
SMRiP29372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110490. 70 interactors.
IntActiP29372. 15 interactors.
MINTiMINT-135511.
STRINGi9606.ENSP00000219431.

Chemistry databases

ChEMBLiCHEMBL3396943.

PTM databases

iPTMnetiP29372.
PhosphoSitePlusiP29372.

Polymorphism and mutation databases

BioMutaiMPG.

Proteomic databases

EPDiP29372.
PaxDbiP29372.
PeptideAtlasiP29372.
PRIDEiP29372.
TopDownProteomicsiP29372-1. [P29372-1]

Protocols and materials databases

DNASUi4350.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219431; ENSP00000219431; ENSG00000103152. [P29372-1]
ENST00000356432; ENSP00000348809; ENSG00000103152. [P29372-4]
ENST00000397817; ENSP00000380918; ENSG00000103152. [P29372-5]
GeneIDi4350.
KEGGihsa:4350.
UCSCiuc002cfm.4. human. [P29372-1]

Organism-specific databases

CTDi4350.
DisGeNETi4350.
GeneCardsiMPG.
HGNCiHGNC:7211. MPG.
HPAiHPA006531.
MIMi156565. gene.
neXtProtiNX_P29372.
OpenTargetsiENSG00000103152.
PharmGKBiPA30917.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4486. Eukaryota.
COG2094. LUCA.
GeneTreeiENSGT00390000009825.
HOGENOMiHOG000224224.
HOVERGENiHBG000019.
InParanoidiP29372.
KOiK03652.
OMAiTHYPLRF.
OrthoDBiEOG091G0NE4.
PhylomeDBiP29372.
TreeFamiTF331768.

Enzyme and pathway databases

BioCyciZFISH:HS02461-MONOMER.
BRENDAi3.2.2.21. 2681.
ReactomeiR-HSA-110330. Recognition and association of DNA glycosylase with site containing an affected purine.
R-HSA-110331. Cleavage of the damaged purine.
R-HSA-110357. Displacement of DNA glycosylase by APEX1.
SABIO-RKP29372.

Miscellaneous databases

ChiTaRSiMPG. human.
EvolutionaryTraceiP29372.
GeneWikiiMPG_(gene).
GenomeRNAii4350.
PROiP29372.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103152.
CleanExiHS_MID1.
HS_MPG.
ExpressionAtlasiP29372. baseline and differential.
GenevisibleiP29372. HS.

Family and domain databases

CDDicd00540. AAG. 1 hit.
Gene3Di3.10.300.10. 1 hit.
HAMAPiMF_00527. 3MGH. 1 hit.
InterProiIPR011034. Formyl_transferase_C-like.
IPR003180. PurDNA_glycsylse.
[Graphical view]
PANTHERiPTHR10429. PTHR10429. 1 hit.
PfamiPF02245. Pur_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
TIGRFAMsiTIGR00567. 3mg. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei3MG_HUMAN
AccessioniPrimary (citable) accession number: P29372
Secondary accession number(s): G5E9E2
, Q13770, Q15275, Q15961, Q5J9I4, Q96BZ6, Q96S33, Q9NNX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 185 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.