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P29366

- BEM1_YEAST

UniProt

P29366 - BEM1_YEAST

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Protein

Bud emergence protein 1

Gene
BEM1, SRO1, YBR200W, YBR1412
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.1 Publication

GO - Molecular functioni

  1. phosphatidylinositol-3-phosphate binding Source: SGD
  2. protein binding Source: IntAct
  3. protein complex scaffold Source: SGD

GO - Biological processi

  1. cell morphogenesis involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29141-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud emergence protein 1
Alternative name(s):
Suppressor of RHO3 protein 1
Gene namesi
Name:BEM1
Synonyms:SRO1
Ordered Locus Names:YBR200W
ORF Names:YBR1412
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR200w.
SGDiS000000404. BEM1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. cytoplasm Source: UniProtKB-KW
  4. cytoskeleton Source: UniProtKB-SubCell
  5. incipient cellular bud site Source: SGD
  6. site of polarized growth Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Bud emergence protein 1PRO_0000064908Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei47 – 471Phosphoserine1 Publication
Modified residuei255 – 2551Phosphoserine1 Publication
Modified residuei258 – 2581Phosphoserine1 Publication
Modified residuei458 – 4581Phosphoserine4 Publications
Modified residuei461 – 4611Phosphoserine3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP29366.
PaxDbiP29366.

Expressioni

Gene expression databases

GenevestigatoriP29366.

Interactioni

Subunit structurei

Interacts with AXL2 and BUD5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BOI1P380415EBI-3508,EBI-3719
BOI2P399696EBI-3508,EBI-3727
CAF130P532803EBI-3508,EBI-23322
CDC24P114339EBI-3508,EBI-4220
CLA4P485623EBI-3508,EBI-4750
MPS1P541993EBI-3508,EBI-11224
STE20Q0349711EBI-3508,EBI-18285

Protein-protein interaction databases

BioGridi32897. 473 interactions.
DIPiDIP-688N.
IntActiP29366. 50 interactions.
MINTiMINT-581819.
STRINGi4932.YBR200W.

Structurei

Secondary structure

1
551
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi160 – 1645
Beta strandi170 – 1734
Beta strandi178 – 1825
Turni189 – 1913
Beta strandi192 – 1954
Beta strandi198 – 2014
Beta strandi205 – 2095
Beta strandi217 – 2204
Helixi222 – 2243
Helixi227 – 2315
Turni232 – 2354
Helixi239 – 2457
Turni246 – 2483
Beta strandi283 – 29412
Beta strandi297 – 30610
Beta strandi311 – 3166
Helixi318 – 33114
Turni333 – 3375
Helixi363 – 38119
Helixi385 – 3884
Helixi391 – 3955
Beta strandi401 – 4044
Beta strandi406 – 4083
Beta strandi479 – 4835
Beta strandi490 – 4945
Helixi500 – 51112
Beta strandi516 – 5205
Beta strandi523 – 5253
Helixi533 – 5419
Beta strandi546 – 5505

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IP9NMR-A472-551[»]
1IPGNMR-A472-551[»]
2CZONMR-A269-418[»]
2KFKNMR-A477-551[»]
2RQVNMR-A156-260[»]
2RQWNMR-A156-260[»]
2V6VX-ray1.50A/B266-413[»]
ProteinModelPortaliP29366.
SMRiP29366. Positions 74-131, 156-260, 276-410, 476-551.

Miscellaneous databases

EvolutionaryTraceiP29366.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 13261SH3 1Add
BLAST
Domaini155 – 21763SH3 2Add
BLAST
Domaini278 – 404127PXAdd
BLAST
Domaini478 – 55174OPRAdd
BLAST

Sequence similaritiesi

Contains 1 OPR domain.
Contains 2 SH3 domains.

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG148927.
HOGENOMiHOG000246638.
KOiK11237.
OMAiKTHESIH.
OrthoDBiEOG7B31WW.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR000270. OPR_PB1.
IPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00312. PX. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29366-1 [UniParc]FASTAAdd to Basket

« Hide

MLKNFKLSKR DSNGSKGRIT SADISTPSHD NGSVIKHIKT VPVRYLSSSS    50
TPVKSQRDSS PKNRHNSKDI TSPEKVIKAK YSYQAQTSKE LSFMEGEFFY 100
VSGDEKDWYK ASNPSTGKEG VVPKTYFEVF DRTKPSSVNG SNSSSRKVTN 150
DSLNMGSLYA IVLYDFKAEK ADELTTYVGE NLFICAHHNC EWFIAKPIGR 200
LGGPGLVPVG FVSIIDIATG YATGNDVIED IKSVNLPTVQ EWKSNIARYK 250
ASNISLGSVE QQQQQSITKP QNKSAKLVDG ELLVKASVES FGLEDEKYWF 300
LVCCELSNGK TRQLKRYYQD FYDLQVQLLD AFPAEAGKLR DAGGQWSKRI 350
MPYIPGPVPY VTNSITKKRK EDLNIYVADL VNLPDYISRS EMVHSLFVVL 400
NNGFDREFER DENQNNIKTL QENDTATFAT ASQTSNFAST NQDNTLTGED 450
LKLNKKLSDL SLSGSKQAPA QSTSGLKTTK IKFYYKDDIF ALMLKGDTTY 500
KELRSKIAPR IDTDNFKLQT KLFDGSGEEI KTDSQVSNII QAKLKISVHD 550
I 551
Length:551
Mass (Da):61,605
Last modified:December 1, 1992 - v1
Checksum:iFB6C525AE9A19181
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63826 Genomic DNA. Translation: CAA45320.1.
Z21487 Genomic DNA. Translation: CAA79687.1.
Z36069 Genomic DNA. Translation: CAA85162.1.
BK006936 Genomic DNA. Translation: DAA07316.1.
PIRiS23400.
RefSeqiNP_009759.3. NM_001178548.3.

Genome annotation databases

EnsemblFungiiYBR200W; YBR200W; YBR200W.
GeneIDi852499.
KEGGisce:YBR200W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63826 Genomic DNA. Translation: CAA45320.1 .
Z21487 Genomic DNA. Translation: CAA79687.1 .
Z36069 Genomic DNA. Translation: CAA85162.1 .
BK006936 Genomic DNA. Translation: DAA07316.1 .
PIRi S23400.
RefSeqi NP_009759.3. NM_001178548.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IP9 NMR - A 472-551 [» ]
1IPG NMR - A 472-551 [» ]
2CZO NMR - A 269-418 [» ]
2KFK NMR - A 477-551 [» ]
2RQV NMR - A 156-260 [» ]
2RQW NMR - A 156-260 [» ]
2V6V X-ray 1.50 A/B 266-413 [» ]
ProteinModelPortali P29366.
SMRi P29366. Positions 74-131, 156-260, 276-410, 476-551.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32897. 473 interactions.
DIPi DIP-688N.
IntActi P29366. 50 interactions.
MINTi MINT-581819.
STRINGi 4932.YBR200W.

Proteomic databases

MaxQBi P29366.
PaxDbi P29366.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR200W ; YBR200W ; YBR200W .
GeneIDi 852499.
KEGGi sce:YBR200W.

Organism-specific databases

CYGDi YBR200w.
SGDi S000000404. BEM1.

Phylogenomic databases

eggNOGi NOG148927.
HOGENOMi HOG000246638.
KOi K11237.
OMAi KTHESIH.
OrthoDBi EOG7B31WW.

Enzyme and pathway databases

BioCyci YEAST:G3O-29141-MONOMER.

Miscellaneous databases

EvolutionaryTracei P29366.
NextBioi 971501.
PROi P29366.

Gene expression databases

Genevestigatori P29366.

Family and domain databases

Gene3Di 3.30.1520.10. 1 hit.
InterProi IPR000270. OPR_PB1.
IPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00564. PB1. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view ]
SMARTi SM00666. PB1. 1 hit.
SM00312. PX. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEi PS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains."
    Chenevert J., Corrado K., Bender A., Pringle J., Herskowitz I.
    Nature 356:77-79(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 10:819-831(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1."
    Chant J., Corrado K., Pringle J.R., Herskowitz I.
    Cell 65:1213-1224(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BUD5.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle."
    Gao X.D., Sperber L.M., Kane S.A., Tong Z., Tong A.H., Boone C., Bi E.
    Mol. Biol. Cell 18:2542-2560(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AXL2, FUNCTION.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-255; SER-258 AND SER-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Structure and ligand recognition of the PB1 domain: a novel protein module binding to the PC motif."
    Terasawa H., Noda Y., Ito T., Hatanaka H., Ichikawa S., Ogura K., Sumimoto H., Inagaki F.
    EMBO J. 20:3947-3956(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 472-551.

Entry informationi

Entry nameiBEM1_YEAST
AccessioniPrimary (citable) accession number: P29366
Secondary accession number(s): D6VQJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6490 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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