Bud emergence protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P29366 (BEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 143. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bud emergence protein 1

Alternative name(s):
Suppressor of RHO3 protein 1

Gene names

Name:	BEM1
Synonyms:	SRO1
Ordered Locus Names:	YBR200W
ORF Names:	YBR1412

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface. Ref.9
Subunit structure	Interacts with AXL2 and BUD5. Ref.5 Ref.9
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	Present with 6490 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 OPR domain. Contains 1 PX (phox homology) domain. Contains 2 SH3 domains.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Repeat SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell communication Inferred from electronic annotation. Source: InterPro cell morphogenesis involved in conjugation with cellular fusion Traceable author statement PubMed 10087263. Source: SGD establishment of cell polarity Traceable author statement PubMed 11113154. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 11927541. Source: SGD cellular bud tip Inferred from direct assay PubMed 11927541. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell incipient cellular bud site Inferred from direct assay PubMed 9128251. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD
Molecular_function	phosphatidylinositol-3-phosphate binding Inferred from direct assay PubMed 11557775. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
BOI2	P39969	3	EBI-3508,EBI-3727
CAF130	P53280	3	EBI-3508,EBI-23322
CDC24	P11433	9	EBI-3508,EBI-4220
STE20	Q03497	6	EBI-3508,EBI-18285

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 551

551

Bud emergence protein 1

PRO_0000064908

Regions

Domain

72 – 132

SH3 1

Domain

155 – 217

SH3 2

Domain

278 – 404

127

Domain

478 – 551

OPR

Amino acid modifications

Modified residue

Phosphoserine Ref.10 Ref.11

Modified residue

Phosphoserine Ref.10 Ref.11

Modified residue

255

Phosphoserine Ref.11

Modified residue

258

Phosphoserine Ref.11

Modified residue

458

Phosphoserine Ref.7 Ref.8 Ref.10 Ref.11

Modified residue

461

Phosphoserine Ref.7 Ref.8 Ref.10 Ref.11

Secondary structure

551

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P29366 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: FB6C525AE9A19181
Show »

FASTA

551

61,605

        10         20         30         40         50         60 
MLKNFKLSKR DSNGSKGRIT SADISTPSHD NGSVIKHIKT VPVRYLSSSS TPVKSQRDSS 

        70         80         90        100        110        120 
PKNRHNSKDI TSPEKVIKAK YSYQAQTSKE LSFMEGEFFY VSGDEKDWYK ASNPSTGKEG 

       130        140        150        160        170        180 
VVPKTYFEVF DRTKPSSVNG SNSSSRKVTN DSLNMGSLYA IVLYDFKAEK ADELTTYVGE 

       190        200        210        220        230        240 
NLFICAHHNC EWFIAKPIGR LGGPGLVPVG FVSIIDIATG YATGNDVIED IKSVNLPTVQ 

       250        260        270        280        290        300 
EWKSNIARYK ASNISLGSVE QQQQQSITKP QNKSAKLVDG ELLVKASVES FGLEDEKYWF 

       310        320        330        340        350        360 
LVCCELSNGK TRQLKRYYQD FYDLQVQLLD AFPAEAGKLR DAGGQWSKRI MPYIPGPVPY 

       370        380        390        400        410        420 
VTNSITKKRK EDLNIYVADL VNLPDYISRS EMVHSLFVVL NNGFDREFER DENQNNIKTL 

       430        440        450        460        470        480 
QENDTATFAT ASQTSNFAST NQDNTLTGED LKLNKKLSDL SLSGSKQAPA QSTSGLKTTK 

       490        500        510        520        530        540 
IKFYYKDDIF ALMLKGDTTY KELRSKIAPR IDTDNFKLQT KLFDGSGEEI KTDSQVSNII 

       550 
QAKLKISVHD I

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains." Chenevert J., Corrado K., Bender A., Pringle J., Herskowitz I. Nature 356:77-79(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family." Mallet L., Bussereau F., Jacquet M. Yeast 10:819-831(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1." Chant J., Corrado K., Pringle J.R., Herskowitz I. Cell 65:1213-1224(1991) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BUD5.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle." Gao X.D., Sperber L.M., Kane S.A., Tong Z., Tong A.H., Boone C., Bi E. Mol. Biol. Cell 18:2542-2560(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AXL2, FUNCTION.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67; SER-72; SER-458 AND SER-461, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67; SER-72; SER-255; SER-258; SER-458 AND SER-461, MASS SPECTROMETRY.
[12]	"Structure and ligand recognition of the PB1 domain: a novel protein module binding to the PC motif." Terasawa H., Noda Y., Ito T., Hatanaka H., Ichikawa S., Ogura K., Sumimoto H., Inagaki F. EMBO J. 20:3947-3956(2001) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 472-551.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X63826 Genomic DNA. Translation: CAA45320.1.
Z21487 Genomic DNA. Translation: CAA79687.1.
Z36069 Genomic DNA. Translation: CAA85162.1.
BK006936 Genomic DNA. Translation: DAA07316.1.

PIR

S23400.

RefSeq

NP_009759.3. NM_001178548.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IP9	NMR	-	A	472-551	[»]
1IPG	NMR	-	A	472-551	[»]
2CZO	NMR	-	A	269-418	[»]
2KFK	NMR	-	A	477-551	[»]
2RQV	NMR	-	A	156-260	[»]
2RQW	NMR	-	A	156-260	[»]
2V6V	X-ray	1.50	A/B	266-413	[»]

ProteinModelPortal

P29366.

SMR

P29366. Positions 73-132, 156-260, 276-410, 476-551.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-688N.

IntAct

P29366. 27 interactions.

MINT

MINT-581819.

STRING

4932.YBR200W.

Proteomic databases

PaxDb

P29366.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR200W; YBR200W; YBR200W.

GeneID

852499.

KEGG

sce:YBR200W.
sce:YBR202W.

Organism-specific databases

CYGD

YBR200w.

SGD

S000000404. BEM1.

Phylogenomic databases

eggNOG

NOG148927.

HOGENOM

HOG000246638.

K02210.
K11237.

OMA

KIAPRID.

OrthoDB

EOG48KVMP.

Gene expression databases

Genevestigator

P29366.

GermOnline

YBR200W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.1520.10. 1 hit.

InterPro

IPR000270. OPR_PB1.
IPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00564. PB1. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]

SMART

SM00666. PB1. 1 hit.
SM00312. PX. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]

SUPFAM

SSF64268. PX. 1 hit.
SSF50044. SH3. 2 hits.

PROSITE

PS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P29366.

NextBio

971501.

Entry information

Entry name

BEM1_YEAST

Accession

Primary (citable) accession number: P29366
Secondary accession number(s): D6VQJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 143 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents