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P29366 (BEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bud emergence protein 1
Alternative name(s):
Suppressor of RHO3 protein 1
Gene names
Name:BEM1
Synonyms:SRO1
Ordered Locus Names:YBR200W
ORF Names:YBR1412
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface. Ref.9

Subunit structure

Interacts with AXL2 and BUD5. Ref.5 Ref.9

Subcellular location

Cytoplasmcytoskeleton.

Miscellaneous

Present with 6490 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 OPR domain.

Contains 1 PX (phox homology) domain.

Contains 2 SH3 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Bud emergence protein 1
PRO_0000064908

Regions

Domain72 – 13261SH3 1
Domain155 – 21763SH3 2
Domain278 – 404127PX
Domain478 – 55174OPR

Amino acid modifications

Modified residue11N-acetylmethionine Ref.12
Modified residue471Phosphoserine Ref.11
Modified residue2551Phosphoserine Ref.11
Modified residue2581Phosphoserine Ref.11
Modified residue4581Phosphoserine Ref.7 Ref.8 Ref.10 Ref.11
Modified residue4611Phosphoserine Ref.7 Ref.8 Ref.10

Secondary structure

.......................................................... 551
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29366 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: FB6C525AE9A19181

FASTA55161,605
        10         20         30         40         50         60 
MLKNFKLSKR DSNGSKGRIT SADISTPSHD NGSVIKHIKT VPVRYLSSSS TPVKSQRDSS 

        70         80         90        100        110        120 
PKNRHNSKDI TSPEKVIKAK YSYQAQTSKE LSFMEGEFFY VSGDEKDWYK ASNPSTGKEG 

       130        140        150        160        170        180 
VVPKTYFEVF DRTKPSSVNG SNSSSRKVTN DSLNMGSLYA IVLYDFKAEK ADELTTYVGE 

       190        200        210        220        230        240 
NLFICAHHNC EWFIAKPIGR LGGPGLVPVG FVSIIDIATG YATGNDVIED IKSVNLPTVQ 

       250        260        270        280        290        300 
EWKSNIARYK ASNISLGSVE QQQQQSITKP QNKSAKLVDG ELLVKASVES FGLEDEKYWF 

       310        320        330        340        350        360 
LVCCELSNGK TRQLKRYYQD FYDLQVQLLD AFPAEAGKLR DAGGQWSKRI MPYIPGPVPY 

       370        380        390        400        410        420 
VTNSITKKRK EDLNIYVADL VNLPDYISRS EMVHSLFVVL NNGFDREFER DENQNNIKTL 

       430        440        450        460        470        480 
QENDTATFAT ASQTSNFAST NQDNTLTGED LKLNKKLSDL SLSGSKQAPA QSTSGLKTTK 

       490        500        510        520        530        540 
IKFYYKDDIF ALMLKGDTTY KELRSKIAPR IDTDNFKLQT KLFDGSGEEI KTDSQVSNII 

       550 
QAKLKISVHD I 

« Hide

References

« Hide 'large scale' references
[1]"A yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains."
Chenevert J., Corrado K., Bender A., Pringle J., Herskowitz I.
Nature 356:77-79(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family."
Mallet L., Bussereau F., Jacquet M.
Yeast 10:819-831(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1."
Chant J., Corrado K., Pringle J.R., Herskowitz I.
Cell 65:1213-1224(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BUD5.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle."
Gao X.D., Sperber L.M., Kane S.A., Tong Z., Tong A.H., Boone C., Bi E.
Mol. Biol. Cell 18:2542-2560(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AXL2, FUNCTION.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-461, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-255; SER-258 AND SER-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Structure and ligand recognition of the PB1 domain: a novel protein module binding to the PC motif."
Terasawa H., Noda Y., Ito T., Hatanaka H., Ichikawa S., Ogura K., Sumimoto H., Inagaki F.
EMBO J. 20:3947-3956(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 472-551.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63826 Genomic DNA. Translation: CAA45320.1.
Z21487 Genomic DNA. Translation: CAA79687.1.
Z36069 Genomic DNA. Translation: CAA85162.1.
BK006936 Genomic DNA. Translation: DAA07316.1.
PIRS23400.
RefSeqNP_009759.3. NM_001178548.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IP9NMR-A472-551[»]
1IPGNMR-A472-551[»]
2CZONMR-A269-418[»]
2KFKNMR-A477-551[»]
2RQVNMR-A156-260[»]
2RQWNMR-A156-260[»]
2V6VX-ray1.50A/B266-413[»]
ProteinModelPortalP29366.
SMRP29366. Positions 74-131, 156-260, 276-410, 476-551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32897. 473 interactions.
DIPDIP-688N.
IntActP29366. 50 interactions.
MINTMINT-581819.
STRING4932.YBR200W.

Proteomic databases

MaxQBP29366.
PaxDbP29366.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR200W; YBR200W; YBR200W.
GeneID852499.
KEGGsce:YBR200W.

Organism-specific databases

CYGDYBR200w.
SGDS000000404. BEM1.

Phylogenomic databases

eggNOGNOG148927.
HOGENOMHOG000246638.
KOK11237.
OMAKTHESIH.
OrthoDBEOG7B31WW.

Enzyme and pathway databases

BioCycYEAST:G3O-29141-MONOMER.

Gene expression databases

GenevestigatorP29366.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR000270. OPR_PB1.
IPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00564. PB1. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
SMARTSM00666. PB1. 1 hit.
SM00312. PX. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEPS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP29366.
NextBio971501.
PROP29366.

Entry information

Entry nameBEM1_YEAST
AccessionPrimary (citable) accession number: P29366
Secondary accession number(s): D6VQJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references