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Protein

Homoserine dehydrogenase

Gene

hom

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.

Enzyme regulationi

Feedback inhibition by threonine.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 3 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (hom)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (hom)
  4. Homoserine kinase (thrB)
  5. Threonine synthase (thrC)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031NADPBy similarity
Binding sitei188 – 1881SubstrateBy similarity
Active sitei203 – 2031Proton donorSequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 168NADPBy similarity

GO - Molecular functioni

  • amino acid binding Source: InterPro
  • homoserine dehydrogenase activity Source: PseudoCAP
  • NADP binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis, Methionine biosynthesis, Threonine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1931-MONOMER.
UniPathwayiUPA00050; UER00063.
UPA00051; UER00465.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine dehydrogenase (EC:1.1.1.3)
Short name:
HDH
Gene namesi
Name:hom
Ordered Locus Names:PA3736
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3736.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Homoserine dehydrogenasePRO_0000066703Add
BLAST

Proteomic databases

PaxDbiP29365.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3736.

Structurei

3D structure databases

ProteinModelPortaliP29365.
SMRiP29365. Positions 24-433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini353 – 42977ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the homoserine dehydrogenase family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105D6E. Bacteria.
COG0460. LUCA.
HOGENOMiHOG000076615.
InParanoidiP29365.
KOiK00003.
OMAiEWIAGII.
OrthoDBiEOG6XM7CQ.
PhylomeDBiP29365.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002912. ACT_dom.
IPR005106. Asp/hSer_DH_NAD-bd.
IPR016204. HDH.
IPR001342. HDH_cat.
IPR019811. HDH_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000098. Homoser_dehydrog. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS01042. HOMOSER_DHGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29365-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPVKVGICG LGTVGGGTFN VLERNAEEIA RRAGRGIEVA QIAARRPNPK
60 70 80 90 100
CDTGATPITA DIFDVACNPE IDVVVELIGG YTLAHELVLK AIENGKHVVT
110 120 130 140 150
ANKALIAVHG NEIFAKAREK GVIVAFEAAV AGGIPVIKAI REGLSANRIN
160 170 180 190 200
WLAGIINGTG NFILSEMREK GRTFPDVLAE AQALGYAEAD PTFDVEGIDA
210 220 230 240 250
AHKLTILASI AFGIPLQFDK AYTEGISKLT SADVNYADAL GYRIKHLGVA
260 270 280 290 300
RRTESGFELR VHPTLIPSDR LIANVNGVMN AVMVNGDAVG STLYYGAGAG
310 320 330 340 350
MEPTASSVVA DLVDVVRAMT SDPENRVPHL AFQPDALSDH PILPIEACES
360 370 380 390 400
AYYLRIQAKD HPGVLAQVAT ILSERGINIE SIMQKEAEEQ DGLVPMILVT
410 420 430
HRVIEQRIND AIAALEALEG VSGPVVRIRV EQLN
Length:434
Mass (Da):46,224
Last modified:December 8, 2000 - v2
Checksum:i68770627B8AD9746
GO

Sequence cautioni

The sequence CAA46167.1 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 855YTLAH → PPWPI in CAA46167 (PubMed:1333566).Curated
Sequence conflicti187 – 1904AEAD → GRGRS in CAA46167 (PubMed:1333566).Curated
Sequence conflicti202 – 2021H → T in CAA46167 (PubMed:1333566).Curated
Sequence conflicti210 – 2145IAFGI → MLRH in CAA46167 (PubMed:1333566).Curated
Sequence conflicti276 – 2772NG → TA in CAA46167 (PubMed:1333566).Curated
Sequence conflicti402 – 4021R → H in CAA46167 (PubMed:1333566).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65033 Genomic DNA. Translation: CAA46167.1. Frameshift.
AE004091 Genomic DNA. Translation: AAG07123.1.
PIRiH83179.
S27979. DEPSHA.
RefSeqiNP_252425.1. NC_002516.2.
WP_003109642.1. NZ_ASJY01000599.1.

Genome annotation databases

EnsemblBacteriaiAAG07123; AAG07123; PA3736.
GeneIDi880337.
KEGGipae:PA3736.
PATRICi19842101. VBIPseAer58763_3908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65033 Genomic DNA. Translation: CAA46167.1. Frameshift.
AE004091 Genomic DNA. Translation: AAG07123.1.
PIRiH83179.
S27979. DEPSHA.
RefSeqiNP_252425.1. NC_002516.2.
WP_003109642.1. NZ_ASJY01000599.1.

3D structure databases

ProteinModelPortaliP29365.
SMRiP29365. Positions 24-433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3736.

Proteomic databases

PaxDbiP29365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07123; AAG07123; PA3736.
GeneIDi880337.
KEGGipae:PA3736.
PATRICi19842101. VBIPseAer58763_3908.

Organism-specific databases

PseudoCAPiPA3736.

Phylogenomic databases

eggNOGiENOG4105D6E. Bacteria.
COG0460. LUCA.
HOGENOMiHOG000076615.
InParanoidiP29365.
KOiK00003.
OMAiEWIAGII.
OrthoDBiEOG6XM7CQ.
PhylomeDBiP29365.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00063.
UPA00051; UER00465.
BioCyciRETL1328306-WGS:GSTH-1931-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002912. ACT_dom.
IPR005106. Asp/hSer_DH_NAD-bd.
IPR016204. HDH.
IPR001342. HDH_cat.
IPR019811. HDH_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000098. Homoser_dehydrog. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS01042. HOMOSER_DHGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes."
    Clepet C., Borne F., Krishnapillai V., Baird C., Patte J.-C., Cami B.
    Mol. Microbiol. 6:3109-3119(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiDHOM_PSEAE
AccessioniPrimary (citable) accession number: P29365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 8, 2000
Last modified: December 9, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.