Reviewed, UniProtKB/Swiss-Prot P29365 (DHOM_PSEAE)

Names and origin

Protein names

Recommended name:
    Homoserine dehydrogenase
      Short name=HDH
    EC=1.1.1.3

Gene names

Name:	hom
Ordered Locus Names:	PA3736

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.
Enzyme regulation	Feedback inhibition by threonine.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Sequence similarities	Belongs to the homoserine dehydrogenase family. Contains 1 ACT domain.
Sequence caution	The sequence CAA46167.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Homoserine dehydrogenase		PRO_0000066703
Regions
	Domain	352 – 427	76	ACT
	Nucleotide binding	9 – 16	8	NADP By similarity
Sites
	Active site	203	1	Proton donor Potential
	Binding site	103	1	NADP By similarity
	Binding site	188	1	Substrate By similarity
Experimental info
	Sequence conflict	81 – 85	5	YTLAH → PPWPI in CAA46167. Ref.1
	Sequence conflict	187 – 190	4	AEAD → GRGRS in CAA46167. Ref.1
	Sequence conflict	202	1	H → T in CAA46167. Ref.1
	Sequence conflict	210 – 214	5	IAFGI → MLRH in CAA46167. Ref.1
	Sequence conflict	276 – 277	2	NG → TA in CAA46167. Ref.1
	Sequence conflict	402	1	R → H in CAA46167. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29365-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 68770627B8AD9746	FASTA	434	46,224
	10 20 30 40 50 60 MKPVKVGICG LGTVGGGTFN VLERNAEEIA RRAGRGIEVA QIAARRPNPK CDTGATPITA 70 80 90 100 110 120 DIFDVACNPE IDVVVELIGG YTLAHELVLK AIENGKHVVT ANKALIAVHG NEIFAKAREK 130 140 150 160 170 180 GVIVAFEAAV AGGIPVIKAI REGLSANRIN WLAGIINGTG NFILSEMREK GRTFPDVLAE 190 200 210 220 230 240 AQALGYAEAD PTFDVEGIDA AHKLTILASI AFGIPLQFDK AYTEGISKLT SADVNYADAL 250 260 270 280 290 300 GYRIKHLGVA RRTESGFELR VHPTLIPSDR LIANVNGVMN AVMVNGDAVG STLYYGAGAG 310 320 330 340 350 360 MEPTASSVVA DLVDVVRAMT SDPENRVPHL AFQPDALSDH PILPIEACES AYYLRIQAKD 370 380 390 400 410 420 HPGVLAQVAT ILSERGINIE SIMQKEAEEQ DGLVPMILVT HRVIEQRIND AIAALEALEG 430 VSGPVVRIRV EQLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes." Clepet C., Borne F., Krishnapillai V., Baird C., Patte J.-C., Cami B. Mol. Microbiol. 6:3109-3119(1992) [PubMed: 1333566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65033 Genomic DNA. Translation: CAA46167.1. Frameshift. AE004091 Genomic DNA. Translation: AAG07123.1.
PIR	H83179. DEPSHA. S27979.
RefSeq	NP_252425.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880337.
GenomeReviews	Gene locus PA3736 in contig AE004091_GR.
KEGG	pae:PA3736.
NMPDR	fig|208964.1.peg.3735.
Organism-specific databases
PseudoCAP	PA3736.
CMR	Search...
Phylogenomic databases
HOGENOM	P29365.
OMA	P29365. LGIVNGT.
Enzyme and pathway databases
BioCyc	PAER208964:PA3736-MON.
BRENDA	1.1.1.3. 354.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR005106. Asp/hSer_DH_NAD-bd. IPR016204. Homoserine_dehydrogenase. IPR001342. Homoserine_dehydrogenase_cat. IPR019811. Homoserine_dehydrogenase_CS. [Graphical view]
Pfam	PF01842. ACT. 1 hit. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000098. Homoser_dehydrog. 1 hit.
PROSITE	PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHOM_PSEAE

Accession

Primary (citable) accession number: P29365

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 8, 2000
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)