Reviewed, UniProtKB/Swiss-Prot P29364 (KHSE_PSEAE)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	PA5495

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00301
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00301
Sequence similarities	Belongs to the pseudomonas-type thrB family.
Sequence caution	The sequence CAA46169.1 differs from that shown. Reason: Frameshift at positions 121 and 201.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Homoserine kinase HAMAP MF_00301		PRO_0000172194

Sequences

	Sequence		Length	Mass (Da)
	P29364-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: D57307AAEE5B60C9	FASTA	316	35,374
	10 20 30 40 50 60 MSVFTPLERS TLEAFLAPYD LGRLRDFRGI AEGSENSNFF VSLEHGEFVL TLVERGPVQD 70 80 90 100 110 120 LPFFIELLDV LHEDGLPVPY ALRTRDGEAL RRLEGKPALL QPRLAGRHER QPNAHHCQEV 130 140 150 160 170 180 GDLLGHLHAA TRGRILERPS DRGLPWMLEQ GANLAPRLPE QARALLAPAL AEIAALDAER 190 200 210 220 230 240 PALPRANLHA DLFRDNVLFD GPHLAGLIDF YNACSGWMLY DLAITLNDWC SNTDGSLDPA 250 260 270 280 290 300 RARALLAAYA NRRPFTALEA EHWPSMLRVA CVRFWLSRLI AAEAFAGQDV LIHDPAEFEI 310 RLAQRQNVEI HLPFAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes." Clepet C., Borne F., Krishnapillai V., Baird C., Patte J.-C., Cami B. Mol. Microbiol. 6:3109-3119(1992) [PubMed: 1333566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65034 Genomic DNA. Translation: CAA46169.1. Frameshift. AE004091 Genomic DNA. Translation: AAG08880.1.
PIR	H82958. S27981.
RefSeq	NP_254182.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	877825.
GenomeReviews	Gene locus PA5495 in contig AE004091_GR.
KEGG	pae:PA5495.
NMPDR	fig|208964.1.peg.5492.
Organism-specific databases
PseudoCAP	PA5495.
CMR	Search...
Phylogenomic databases
HOGENOM	P29364.
OMA	ADLFRDN.
Enzyme and pathway databases
BioCyc	PAER208964:PA5495-MON.
BRENDA	2.7.1.39. 354.
Family and domain databases
HAMAP	MF_00301. [Tree]
InterPro	IPR002575. APH_trans. IPR005280. Homoserine_Kinase_ThrB. [Graphical view]
Pfam	PF01636. APH. 1 hit. [Graphical view]
TIGRFAMs	TIGR00938. thrB_alt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KHSE_PSEAE

Accession

Primary (citable) accession number: P29364
Secondary accession number(s): Q9HT78

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 11, 2001
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)