Reviewed, UniProtKB/Swiss-Prot P29363 (THRC_PSEAE)

Names and origin

Protein names

Recommended name:
    Threonine synthase
    EC=4.2.3.1

Gene names

Name:	thrC
Ordered Locus Names:	PA3735

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O-phospho-L-homoserine + H2O = L-threonine + phosphate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.
Sequence similarities	Belongs to the serine/threonine dehydratase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro threonine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Threonine synthase		PRO_0000185639
Amino acid modifications
	Modified residue	112	1	N6-(pyridoxal phosphate)lysine Probable
Experimental info
	Sequence conflict	88 – 101	14	VAPLR…NEWVL → SGAAAPVERRTNGCV Ref.1
	Sequence conflict	151 – 155	5	GCRRC → AAAVA in CAA46168. Ref.1
	Sequence conflict	163 – 164	2	MH → ID in CAA46168. Ref.1
	Sequence conflict	173	1	Q → E in CAA46168. Ref.1
	Sequence conflict	181	1	L → H in CAA46168. Ref.1
	Sequence conflict	280	1	T → R in CAA46168. Ref.1
	Sequence conflict	294 – 304	11	RYDKDTLHPSL → ASTRHTLTPSV in CAA46168. Ref.1
	Sequence conflict	465	1	R → P in CAA46168. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29363-1 [UniParc]. Last modified January 11, 2001. Version 3. Checksum: 224032B86272C79C	FASTA	469	51,795
	10 20 30 40 50 60 MRYISTRGQA PALNFEDVLL AGLASDGGLY VPENLPRFTL EEIASWVGLP YHELAFRVMR 70 80 90 100 110 120 PFVAGSIADA DFKKILEETY GVFAHDAVAP LRQLNGNEWV LELFHGPTLA FKDFALQLLG 130 140 150 160 170 180 RLLDHVLAKR GERVVIMGAT SGDTGSAAIE GCRRCDNVDI FIMHPHNRVS EVQRRQMTTI 190 200 210 220 230 240 LGDNIHNIAI EGNFDDCQEM VKASFADQGF LKGTRLVAVN SINWARIMAQ IVYYFHAALQ 250 260 270 280 290 300 LGAPHRSVAF SVPTGNFGDI FAGYLARNMG LPVSQLIVAT NRNDILHRFM SGNRYDKDTL 310 320 330 340 350 360 HPSLSPSMDI MVSSNFERLL FDLHGRNGKA VAELLDAFKA SGKLSVEDQR WTEARKLFDS 370 380 390 400 410 420 LAVSDEQTCE TIAEVYRSSG ELLDPHTAIG VRAARECRRS LSVPMVTLGT AHPVKFPEAV 430 440 450 460 EKAGIGQAPA LPAHLADLFE REERCTVLPN ELAKVQAFVS QHGNRGKPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes." Clepet C., Borne F., Krishnapillai V., Baird C., Patte J.-C., Cami B. Mol. Microbiol. 6:3109-3119(1992) [PubMed: 1333566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65033 Genomic DNA. Translation: CAA46168.1. Different initiation. AE004091 Genomic DNA. Translation: AAG07122.1.
PIR	G83179. SYPSRA. S27980.
RefSeq	NP_252424.1.
3D structure databases
HSSP	HSSP built from PDB template 1KL7 based on UniProtKB P16120.
ModBase	Search...
Genome annotation databases
GeneID	880336.
GenomeReviews	Gene locus PA3735 in contig AE004091_GR.
KEGG	pae:PA3735.
NMPDR	fig|208964.1.peg.3734.
Organism-specific databases
PseudoCAP	PA3735.
CMR	Search...
Phylogenomic databases
HOGENOM	P29363.
OMA	YYFTSAV.
Enzyme and pathway databases
BioCyc	PAER208964:PA3735-MON.
BRENDA	4.2.3.1. 354.
Family and domain databases
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS. IPR004450. Thr_synthase. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR00260. thrC. 1 hit.
PROSITE	PS00165. DEHYDRATASE_SER_THR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THRC_PSEAE

Accession

Primary (citable) accession number: P29363
Secondary accession number(s): Q9HXQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 11, 2001
Last modified:	November 3, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)