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Protein

Threonine synthase

Gene

thrC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.By similarity

Catalytic activityi

O-phospho-L-homoserine + H2O = L-threonine + phosphate.

Cofactori

Pathway: L-threonine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (hom)
  4. Homoserine kinase (thrB)
  5. Threonine synthase (thrC)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-999-MONOMER.
UniPathwayiUPA00050; UER00065.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine synthase (EC:4.2.3.1)
Short name:
TS
Gene namesi
Name:thrC
Ordered Locus Names:PA3735
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3735.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Threonine synthasePRO_0000185639Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei112 – 1121N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3735.

Structurei

3D structure databases

ProteinModelPortaliP29363.
SMRiP29363. Positions 1-462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the threonine synthase family.Curated

Phylogenomic databases

eggNOGiCOG0498.
HOGENOMiHOG000230745.
InParanoidiP29363.
KOiK01733.
OMAiNVHALAI.
OrthoDBiEOG65BDJX.
PhylomeDBiP29363.

Family and domain databases

Gene3Di3.90.1380.10. 1 hit.
InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR029144. Thr_synth_N.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYISTRGQA PALNFEDVLL AGLASDGGLY VPENLPRFTL EEIASWVGLP
60 70 80 90 100
YHELAFRVMR PFVAGSIADA DFKKILEETY GVFAHDAVAP LRQLNGNEWV
110 120 130 140 150
LELFHGPTLA FKDFALQLLG RLLDHVLAKR GERVVIMGAT SGDTGSAAIE
160 170 180 190 200
GCRRCDNVDI FIMHPHNRVS EVQRRQMTTI LGDNIHNIAI EGNFDDCQEM
210 220 230 240 250
VKASFADQGF LKGTRLVAVN SINWARIMAQ IVYYFHAALQ LGAPHRSVAF
260 270 280 290 300
SVPTGNFGDI FAGYLARNMG LPVSQLIVAT NRNDILHRFM SGNRYDKDTL
310 320 330 340 350
HPSLSPSMDI MVSSNFERLL FDLHGRNGKA VAELLDAFKA SGKLSVEDQR
360 370 380 390 400
WTEARKLFDS LAVSDEQTCE TIAEVYRSSG ELLDPHTAIG VRAARECRRS
410 420 430 440 450
LSVPMVTLGT AHPVKFPEAV EKAGIGQAPA LPAHLADLFE REERCTVLPN
460
ELAKVQAFVS QHGNRGKPL
Length:469
Mass (Da):51,795
Last modified:January 11, 2001 - v3
Checksum:i224032B86272C79C
GO

Sequence cautioni

The sequence CAA46168.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 10114VAPLR…NEWVL → SGAAAPVERRTNGCV (PubMed:1333566).CuratedAdd
BLAST
Sequence conflicti151 – 1555GCRRC → AAAVA in CAA46168 (PubMed:1333566).Curated
Sequence conflicti163 – 1642MH → ID in CAA46168 (PubMed:1333566).Curated
Sequence conflicti173 – 1731Q → E in CAA46168 (PubMed:1333566).Curated
Sequence conflicti181 – 1811L → H in CAA46168 (PubMed:1333566).Curated
Sequence conflicti280 – 2801T → R in CAA46168 (PubMed:1333566).Curated
Sequence conflicti294 – 30411RYDKDTLHPSL → ASTRHTLTPSV in CAA46168 (PubMed:1333566).CuratedAdd
BLAST
Sequence conflicti465 – 4651R → P in CAA46168 (PubMed:1333566).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65033 Genomic DNA. Translation: CAA46168.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG07122.1.
PIRiG83179.
S27980. SYPSRA.
RefSeqiNP_252424.1. NC_002516.2.
WP_003113831.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07122; AAG07122; PA3735.
GeneIDi880336.
KEGGipae:PA3735.
PATRICi19842099. VBIPseAer58763_3907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65033 Genomic DNA. Translation: CAA46168.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG07122.1.
PIRiG83179.
S27980. SYPSRA.
RefSeqiNP_252424.1. NC_002516.2.
WP_003113831.1. NC_002516.2.

3D structure databases

ProteinModelPortaliP29363.
SMRiP29363. Positions 1-462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07122; AAG07122; PA3735.
GeneIDi880336.
KEGGipae:PA3735.
PATRICi19842099. VBIPseAer58763_3907.

Organism-specific databases

PseudoCAPiPA3735.

Phylogenomic databases

eggNOGiCOG0498.
HOGENOMiHOG000230745.
InParanoidiP29363.
KOiK01733.
OMAiNVHALAI.
OrthoDBiEOG65BDJX.
PhylomeDBiP29363.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00065.
BioCyciRETL1328306-WGS:GSTH-999-MONOMER.

Family and domain databases

Gene3Di3.90.1380.10. 1 hit.
InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR029144. Thr_synth_N.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes."
    Clepet C., Borne F., Krishnapillai V., Baird C., Patte J.-C., Cami B.
    Mol. Microbiol. 6:3109-3119(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiTHRC_PSEAE
AccessioniPrimary (citable) accession number: P29363
Secondary accession number(s): Q9HXQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 11, 2001
Last modified: June 24, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.