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P29356 (ALF_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase, cytoplasmic isozyme

EC=4.1.2.13
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Fructose-bisphosphate aldolase, cytoplasmic isozyme
PRO_0000216925

Sites

Active site1831Proton acceptor By similarity
Active site2251Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site531Substrate By similarity
Binding site1421Substrate By similarity
Site3571Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Sequences

Sequence LengthMass (Da)Tools
P29356 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 33150E3D80B1C6DE

FASTA35738,471
        10         20         30         40         50         60 
MTAYRGKYAD ELIANASYIA TPGKVILAAD ESTGTIGKRF PSINVENVES NRRALRELLF 

        70         80         90        100        110        120 
TTPGALPYLS GVILFEETLY QKTADGKPFV DAMKDGGVLP GIKVDKGTVE LAGTNGETTT 

       130        140        150        160        170        180 
QGLDGLAQRC AQYYTAGARF AKWRAVLKIG PTEPSPLAIL ENANGLARYG IICQENGLVP 

       190        200        210        220        230        240 
IVEPEILVDG THDIDRCAEV SERVLAACYK ALNDHHVLLE GTSLKPNIVT PGSESKKVTP 

       250        260        270        280        290        300 
EVIAEYTVRT LQRTVPQAVP GVMFLSGGQS EEEATLNLNA MNKLETKKPW TLSFSYGRAL 

       310        320        330        340        350 
QQSTLKAWQG KEENVAKAQE VFLARAKGNS EATLGKYQGG AGGADASESL HVKDYKY 

« Hide

References

[1]"Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach."
Pelzer-Reith B., Penger A., Schnarrenberger C.
Plant Mol. Biol. 21:331-340(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65742 mRNA. Translation: CAA46649.1.
PIRADSPAC. S31091.

3D structure databases

ProteinModelPortalP29356.
SMRP29356. Positions 11-357.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12898.
SABIO-RKP29356.
UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_SPIOL
AccessionPrimary (citable) accession number: P29356
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways