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P29356

- ALF_SPIOL

UniProt

P29356 - ALF_SPIOL

Protein

Fructose-bisphosphate aldolase, cytoplasmic isozyme

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531SubstrateBy similarity
    Binding sitei142 – 1421SubstrateBy similarity
    Active sitei183 – 1831Proton acceptorBy similarity
    Active sitei225 – 2251Schiff-base intermediate with dihydroxyacetone-PBy similarity
    Sitei357 – 3571Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12898.
    SABIO-RKP29356.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase, cytoplasmic isozyme (EC:4.1.2.13)
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357Fructose-bisphosphate aldolase, cytoplasmic isozymePRO_0000216925Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP29356.
    SMRiP29356. Positions 11-357.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29356-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAYRGKYAD ELIANASYIA TPGKVILAAD ESTGTIGKRF PSINVENVES    50
    NRRALRELLF TTPGALPYLS GVILFEETLY QKTADGKPFV DAMKDGGVLP 100
    GIKVDKGTVE LAGTNGETTT QGLDGLAQRC AQYYTAGARF AKWRAVLKIG 150
    PTEPSPLAIL ENANGLARYG IICQENGLVP IVEPEILVDG THDIDRCAEV 200
    SERVLAACYK ALNDHHVLLE GTSLKPNIVT PGSESKKVTP EVIAEYTVRT 250
    LQRTVPQAVP GVMFLSGGQS EEEATLNLNA MNKLETKKPW TLSFSYGRAL 300
    QQSTLKAWQG KEENVAKAQE VFLARAKGNS EATLGKYQGG AGGADASESL 350
    HVKDYKY 357
    Length:357
    Mass (Da):38,471
    Last modified:December 1, 1992 - v1
    Checksum:i33150E3D80B1C6DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65742 mRNA. Translation: CAA46649.1.
    PIRiS31091. ADSPAC.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65742 mRNA. Translation: CAA46649.1 .
    PIRi S31091. ADSPAC.

    3D structure databases

    ProteinModelPortali P29356.
    SMRi P29356. Positions 11-357.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    BioCyci MetaCyc:MONOMER-12898.
    SABIO-RK P29356.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach."
      Pelzer-Reith B., Penger A., Schnarrenberger C.
      Plant Mol. Biol. 21:331-340(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Leaf.

    Entry informationi

    Entry nameiALF_SPIOL
    AccessioniPrimary (citable) accession number: P29356
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3