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Protein

Sex muscle abnormal protein 5

Gene

sem-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which modulates signaling mediated by several receptor tyrosine kinases such as egl-15 and let-23 probably acting upstream of let-60/ras. Negatively regulates vulva induction probably downstream of let-23 (PubMed:1372395, PubMed:16547100). Involved in sex myoblast migration (PubMed:1372395). Negatively regulates fluid homeostasis probably downstream of egl-15 (PubMed:1372395, PubMed:11689700). During the formation of neuromuscular junctions at the larval stage, negatively regulates membrane protrusion from body wall muscles probably downstream of egl-15 (PubMed:16495308). Involved in let-23-mediated regulation of fertility independently of let-60/Ras (PubMed:16547100). Negatively regulates daf-2-mediated repression of dauer formation (PubMed:16547100). Plays a role in nicotinic acetylcholine receptor (nAChR)-mediated sensitivity to nicotine (PubMed:15990870). Regulates synaptic levels of nAchR subunit lev-1 in the nerve cord (PubMed:15990870). May play a role in oocyte development upstream of let-60/Ras and the MAP kinase pathway (PubMed:12169634).6 Publications

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: WormBase
  • SH3/SH2 adaptor activity Source: WormBase

GO - Biological processi

  • cell migration Source: WormBase
  • male genitalia development Source: WormBase
  • muscle organ development Source: WormBase
  • nematode larval development Source: WormBase
  • regulation of cell projection organization Source: WormBase
  • regulation of vulval development Source: WormBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-CEL-167044. Signalling to RAS.
R-CEL-179812. GRB2 events in EGFR signaling.
R-CEL-180336. SHC1 events in EGFR signaling.
R-CEL-182971. EGFR downregulation.
R-CEL-2424491. DAP12 signaling.
R-CEL-2428933. SHC-related events triggered by IGF1R.
R-CEL-2871796. FCERI mediated MAPK activation.
R-CEL-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-CEL-375165. NCAM signaling for neurite out-growth.
R-CEL-5654699. SHC-mediated cascade:FGFR2.
R-CEL-5654700. FRS-mediated FGFR2 signaling.
R-CEL-5654704. SHC-mediated cascade:FGFR3.
R-CEL-5654706. FRS-mediated FGFR3 signaling.
R-CEL-5654727. Negative regulation of FGFR2 signaling.
R-CEL-5654732. Negative regulation of FGFR3 signaling.
R-CEL-5673001. RAF/MAP kinase cascade.
R-CEL-74749. Signal attenuation.
R-CEL-74751. Insulin receptor signalling cascade.
R-CEL-912631. Regulation of signaling by CBL.
SignaLinkiP29355.

Names & Taxonomyi

Protein namesi
Recommended name:
Sex muscle abnormal protein 5
Gene namesi
Name:sem-5
ORF Names:C14F5.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC14F5.5; CE01784; WBGene00004774; sem-5.

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown or RNAi-mediated knockdown in body wall muscles causes ectopic membrane extensions from body wall muscles.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491P → L in n1619; lethal at the larval stage, mild defect in vulva development and partially impaired sex myoblast migration. Rescues fluid accumulation in crl-1 e1745ts mutant. 1 Publication
Mutagenesisi87 – 871R → Q in ay73; partial rod-like larval lethality. Surviving mutant have a thin body and no vulva. Rescues fluid accumulation in clr-1 e1745ts mutant. 1 Publication
Mutagenesisi90 – 901E → K in n1779; mild defect in vulva development and partially impaired sex myoblast migration. Causes ectopic muscle membrane extension. Reduced lev-1 synaptic levels. Moderate increase in resistance to nicotine-induced paralysis. Rescues fluid accumulation in crl-1 e1745ts mutant. 3 Publications
Mutagenesisi91 – 911S → N in n1781; no obvious defects. Partially rescues fluid accumulation in crl-1 e1745ts mutant. 1 Publication
Mutagenesisi201 – 2011G → R in n2195; loss of interaction with soc-1. Partially restores normal vulva induction in let-60 n1046gf mutant. Partially rescues fluid accumulation in crl-1 e1745ts mutant. Partially prevents constitutive dauer formation in daf-2 m577 mutant. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Sex muscle abnormal protein 5PRO_0000088212Add
BLAST

Proteomic databases

EPDiP29355.
PaxDbiP29355.
PRIDEiP29355.

Expressioni

Tissue specificityi

Expressed in body wall muscles, pharynx, intestine and hypodermis.1 Publication

Interactioni

Subunit structurei

Interacts (probably via SH3 domain 2) with soc-1 (via C-terminus).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Sos1Q622452EBI-315286,EBI-1693From a different organism.

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: WormBase
  • SH3/SH2 adaptor activity Source: WormBase

Protein-protein interaction databases

BioGridi45979. 113 interactions.
DIPiDIP-27394N.
IntActiP29355. 27 interactions.
MINTiMINT-114046.
STRINGi6239.C14F5.5.

Structurei

Secondary structure

1
228
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi158 – 1636Combined sources
Beta strandi169 – 1724Combined sources
Beta strandi180 – 1856Combined sources
Beta strandi187 – 19610Combined sources
Beta strandi199 – 2046Combined sources
Helixi205 – 2073Combined sources
Beta strandi208 – 2125Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K76NMR-A155-214[»]
1KFZNMR-A155-214[»]
1SEMX-ray2.00A/B155-212[»]
2SEMX-ray2.20A/B155-214[»]
3SEMX-ray2.20A/B155-214[»]
ProteinModelPortaliP29355.
SMRiP29355. Positions 1-55, 60-151, 155-212.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29355.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5858SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 15293SH2PROSITE-ProRule annotationAdd
BLAST
Domaini154 – 21360SH3 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3601. Eukaryota.
ENOG410XR1G. LUCA.
GeneTreeiENSGT00820000126999.
HOGENOMiHOG000251625.
InParanoidiP29355.
KOiK04364.
OMAiYDFTPQE.
OrthoDBiEOG75F4F6.
PhylomeDBiP29355.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVAEHDFQ AGSPDELSFK RGNTLKVLNK DEDPHWYKAE LDGNEGFIPS
60 70 80 90 100
NYIRMTECNW YLGKITRNDA EVLLKKPTVR DGHFLVRQCE SSPGEFSISV
110 120 130 140 150
RFQDSVQHFK VLRDQNGKYY LWAVKFNSLN ELVAYHRTAS VSRTHTILLS
160 170 180 190 200
DMNVETKFVQ ALFDFNPQES GELAFKRGDV ITLINKDDPN WWEGQLNNRR
210 220
GIFPSNYVCP YNSNKSNSNV APGFNFGN
Length:228
Mass (Da):26,210
Last modified:December 1, 1992 - v1
Checksum:i2D684C9520646C41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S88446 mRNA. Translation: AAB21850.1.
FO080456 Genomic DNA. Translation: CCD63850.1.
PIRiS25730.
RefSeqiNP_509342.1. NM_076941.5.
UniGeneiCel.19703.

Genome annotation databases

EnsemblMetazoaiC14F5.5; C14F5.5; WBGene00004774.
GeneIDi181055.
KEGGicel:CELE_C14F5.5.
UCSCiC14F5.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S88446 mRNA. Translation: AAB21850.1.
FO080456 Genomic DNA. Translation: CCD63850.1.
PIRiS25730.
RefSeqiNP_509342.1. NM_076941.5.
UniGeneiCel.19703.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K76NMR-A155-214[»]
1KFZNMR-A155-214[»]
1SEMX-ray2.00A/B155-212[»]
2SEMX-ray2.20A/B155-214[»]
3SEMX-ray2.20A/B155-214[»]
ProteinModelPortaliP29355.
SMRiP29355. Positions 1-55, 60-151, 155-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45979. 113 interactions.
DIPiDIP-27394N.
IntActiP29355. 27 interactions.
MINTiMINT-114046.
STRINGi6239.C14F5.5.

Proteomic databases

EPDiP29355.
PaxDbiP29355.
PRIDEiP29355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC14F5.5; C14F5.5; WBGene00004774.
GeneIDi181055.
KEGGicel:CELE_C14F5.5.
UCSCiC14F5.5. c. elegans.

Organism-specific databases

CTDi181055.
WormBaseiC14F5.5; CE01784; WBGene00004774; sem-5.

Phylogenomic databases

eggNOGiKOG3601. Eukaryota.
ENOG410XR1G. LUCA.
GeneTreeiENSGT00820000126999.
HOGENOMiHOG000251625.
InParanoidiP29355.
KOiK04364.
OMAiYDFTPQE.
OrthoDBiEOG75F4F6.
PhylomeDBiP29355.

Enzyme and pathway databases

ReactomeiR-CEL-167044. Signalling to RAS.
R-CEL-179812. GRB2 events in EGFR signaling.
R-CEL-180336. SHC1 events in EGFR signaling.
R-CEL-182971. EGFR downregulation.
R-CEL-2424491. DAP12 signaling.
R-CEL-2428933. SHC-related events triggered by IGF1R.
R-CEL-2871796. FCERI mediated MAPK activation.
R-CEL-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-CEL-375165. NCAM signaling for neurite out-growth.
R-CEL-5654699. SHC-mediated cascade:FGFR2.
R-CEL-5654700. FRS-mediated FGFR2 signaling.
R-CEL-5654704. SHC-mediated cascade:FGFR3.
R-CEL-5654706. FRS-mediated FGFR3 signaling.
R-CEL-5654727. Negative regulation of FGFR2 signaling.
R-CEL-5654732. Negative regulation of FGFR3 signaling.
R-CEL-5673001. RAF/MAP kinase cascade.
R-CEL-74749. Signal attenuation.
R-CEL-74751. Insulin receptor signalling cascade.
R-CEL-912631. Regulation of signaling by CBL.
SignaLinkiP29355.

Miscellaneous databases

EvolutionaryTraceiP29355.
PROiP29355.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains."
    Clark S.G., Stern M.J., Horvitz H.R.
    Nature 356:340-344(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF PRO-49; GLU-90; SER-91 AND GLY-201.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "The Caenorhabditis elegans EGL-15 signaling pathway implicates a DOS-like multisubstrate adaptor protein in fibroblast growth factor signal transduction."
    Schutzman J.L., Borland C.Z., Newman J.C., Robinson M.K., Kokel M., Stern M.J.
    Mol. Cell. Biol. 21:8104-8116(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-87.
  4. "The C.elegans MAPK phosphatase LIP-1 is required for the G(2)/M meiotic arrest of developing oocytes."
    Hajnal A., Berset T.
    EMBO J. 21:4317-4326(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Identification and characterization of novel nicotinic receptor-associated proteins in Caenorhabditis elegans."
    Gottschalk A., Almedom R.B., Schedletzky T., Anderson S.D., Yates J.R. III, Schafer W.R.
    EMBO J. 24:2566-2578(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-90.
  6. "FGF negatively regulates muscle membrane extension in Caenorhabditis elegans."
    Dixon S.J., Alexander M., Fernandes R., Ricker N., Roy P.J.
    Development 133:1263-1275(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-90.
  7. "The adaptor protein soc-1/Gab1 modifies growth factor receptor output in Caenorhabditis elegans."
    Hopper N.A.
    Genetics 173:163-175(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SOC-1, MUTAGENESIS OF GLY-201.
  8. "Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains."
    Lim W.A., Richards F.M., Fox R.O.
    Nature 372:375-379(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 153-214.
  9. "Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors."
    Nguyen J.T., Turck C.W., Cohen F.E., Zuckermann R.N., Lim W.A.
    Science 282:2088-2092(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 155-214.

Entry informationi

Entry nameiSEM5_CAEEL
AccessioniPrimary (citable) accession number: P29355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 6, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.