Reviewed, UniProtKB/Swiss-Prot P29355 (SEM5_CAEEL)

Names and origin

Protein names

Recommended name:
    Sex muscle abnormal protein 5

Gene names

Name:	sem-5
ORF Names:	C14F5.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts both in vulval induction and sex myoblast migration. Presumably interacts with the kinase receptor let-23 and with a target that modifies the Ras-like protein let-60.
Sequence similarities	Belongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.

Ontologies

Keywords
Domain	Repeat SH2 domain SH3 domain
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase negative regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9XVW8	1	EBI-315286,EBI-315348

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 228

228

Sex muscle abnormal protein 5

PRO_0000088212

Regions

Domain

1 – 58

58

SH3 1

Domain

60 – 152

93

SH2

Domain

154 – 213

60

SH3 2

Experimental info

Mutagenesis

49

1

P → L in N1619. Ref.1

Mutagenesis

90

1

E → K in N1779. Ref.1

Mutagenesis

91

1

S → N in N1781. Ref.1

Mutagenesis

201

1

G → R in N2195. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

228

Helix Strand Turn

Details...

Beta strand

158 – 163

6

Beta strand

180 – 185

6

Beta strand

187 – 196

10

Beta strand

199 – 204

6

Helix

205 – 207

3

Beta strand

208 – 212

5

Sequences

	Sequence		Length	Mass (Da)
	P29355-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 2D684C9520646C41	FASTA	228	26,210
	10 20 30 40 50 60 MEAVAEHDFQ AGSPDELSFK RGNTLKVLNK DEDPHWYKAE LDGNEGFIPS NYIRMTECNW 70 80 90 100 110 120 YLGKITRNDA EVLLKKPTVR DGHFLVRQCE SSPGEFSISV RFQDSVQHFK VLRDQNGKYY 130 140 150 160 170 180 LWAVKFNSLN ELVAYHRTAS VSRTHTILLS DMNVETKFVQ ALFDFNPQES GELAFKRGDV 190 200 210 220 ITLINKDDPN WWEGQLNNRR GIFPSNYVCP YNSNKSNSNV APGFNFGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains." Clark S.G., Stern M.J., Horvitz H.R. Nature 356:340-344(1992) [PubMed: 1372395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF PRO-49; GLU-90; SER-91 AND GLY-201.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains." Lim W.A., Richards F.M., Fox R.O. Nature 372:375-379(1994) [PubMed: 7802869] [Abstract] Cited for: STRUCTURE BY NMR OF 153-214.
[4]	"Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors." Nguyen J.T., Turck C.W., Cohen F.E., Zuckermann R.N., Lim W.A. Science 282:2088-2092(1998) [PubMed: 9851931] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 155-214.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

S88446 mRNA. Translation: AAB21850.1.
U29082 Genomic DNA. Translation: AAA68405.1.

PIR

S25730.

RefSeq

NP_509342.1.

UniGene

Cel.19703

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K76	NMR	-	A	155-214	[»]
1KFZ	NMR	-	A	155-214	[»]
1SEM	X-ray	2.00	A/B	155-212	[»]
2SEM	X-ray	2.20	A/B	155-214	[»]
3SEM	X-ray	2.20	A/B	155-214	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:27394N.

IntAct

P29355. 13 interactions.

Genome annotation databases

Ensembl

C14F5.5. Caenorhabditis elegans. [Contig view]

GeneID

181055.

KEGG

cel:C14F5.5.

NMPDR

fig|6239.3.peg.23897.

Organism-specific databases

WormBase

WBGene00004774. sem-5.

WormPep

C14F5.5. CE01784. [WorfDB]

Phylogenomic databases

OMA

P29355. AFLIRIS.

Gene expression databases

ArrayExpress

P29355.

Family and domain databases

InterPro

IPR000108. Neu_cyt_fact_2.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]

Gene3D

G3DSA:3.30.505.10. SH2. 1 hit.

Pfam

PF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]

PRINTS

PR00499. P67PHOX.
PR00401. SH2DOMAIN.

ProDom

PD000093. SH2. 1 hit.
PD000066. SH3. 2 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]

PROSITE

PS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

912202.

Entry information

Entry name

SEM5_CAEEL

Accession

Primary (citable) accession number: P29355

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project