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Protein

Sex muscle abnormal protein 5

Gene

sem-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein which modulates signaling mediated by several receptor tyrosine kinases such as egl-15 and let-23 probably acting upstream of let-60/ras. Negatively regulates vulva induction probably downstream of let-23 (PubMed:1372395, PubMed:16547100). Involved in sex myoblast migration (PubMed:1372395, PubMed:9073451). Negatively regulates fluid homeostasis probably downstream of egl-15 (PubMed:1372395, PubMed:11689700). During the formation of neuromuscular junctions at the larval stage, negatively regulates membrane protrusion from body wall muscles probably downstream of egl-15 (PubMed:16495308). Involved in cytoskeleton dynamics and is recruited by mig-13 to the leading edge of Q neuroblasts and their descendants to signal downstream to activate the wsp-1 pathway and direct migration along the anteroposterior body axis during larval development (PubMed:27780040). Involved in let-23-mediated regulation of fertility independently of let-60/Ras (PubMed:16547100). Negatively regulates daf-2-mediated repression of dauer formation (PubMed:16547100). Plays a role in nicotinic acetylcholine receptor (nAChR)-mediated sensitivity to nicotine (PubMed:15990870). Regulates synaptic levels of nAchR subunit lev-1 in the nerve cord (PubMed:15990870). May play a role in oocyte development upstream of let-60/Ras and the MAP kinase pathway (PubMed:12169634).8 Publications

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: WormBase
  • non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  • SH3/SH2 adaptor activity Source: WormBase

GO - Biological processi

  • cell differentiation Source: GO_Central
  • cell migration Source: WormBase
  • innate immune response Source: GO_Central
  • male genitalia development Source: WormBase
  • muscle organ development Source: WormBase
  • nematode larval development Source: WormBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • Ras protein signal transduction Source: InterPro
  • regulation of cell projection organization Source: WormBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of vulval development Source: WormBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Enzyme and pathway databases

ReactomeiR-CEL-1250347 SHC1 events in ERBB4 signaling
R-CEL-167044 Signalling to RAS
R-CEL-179812 GRB2 events in EGFR signaling
R-CEL-180292 GAB1 signalosome
R-CEL-180336 SHC1 events in EGFR signaling
R-CEL-182971 EGFR downregulation
R-CEL-1963640 GRB2 events in ERBB2 signaling
R-CEL-2179392 EGFR Transactivation by Gastrin
R-CEL-2424491 DAP12 signaling
R-CEL-2428933 SHC-related events triggered by IGF1R
R-CEL-2871796 FCERI mediated MAPK activation
R-CEL-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-CEL-375165 NCAM signaling for neurite out-growth
R-CEL-5654688 SHC-mediated cascade:FGFR1
R-CEL-5654693 FRS-mediated FGFR1 signaling
R-CEL-5654699 SHC-mediated cascade:FGFR2
R-CEL-5654700 FRS-mediated FGFR2 signaling
R-CEL-5654704 SHC-mediated cascade:FGFR3
R-CEL-5654706 FRS-mediated FGFR3 signaling
R-CEL-5654712 FRS-mediated FGFR4 signaling
R-CEL-5654719 SHC-mediated cascade:FGFR4
R-CEL-5654727 Negative regulation of FGFR2 signaling
R-CEL-5654732 Negative regulation of FGFR3 signaling
R-CEL-5673001 RAF/MAP kinase cascade
R-CEL-6807004 Negative regulation of MET activity
R-CEL-74749 Signal attenuation
R-CEL-74751 Insulin receptor signalling cascade
R-CEL-8851805 MET activates RAS signaling
R-CEL-8851907 MET activates PI3K/AKT signaling
R-CEL-8856825 Cargo recognition for clathrin-mediated endocytosis
R-CEL-8856828 Clathrin-mediated endocytosis
R-CEL-8875555 MET activates RAP1 and RAC1
R-CEL-8875656 MET receptor recycling
R-CEL-912631 Regulation of signaling by CBL
SignaLinkiP29355

Names & Taxonomyi

Protein namesi
Recommended name:
Sex muscle abnormal protein 5
Gene namesi
Name:sem-5
ORF Names:C14F5.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC14F5.5 ; CE01784 ; WBGene00004774 ; sem-5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown or RNAi-mediated knockdown in body wall muscles causes ectopic membrane extensions from body wall muscles.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49P → L in n1619; lethal at the larval stage, mild defect in vulva development and partially impaired sex myoblast migration. Rescues fluid accumulation in crl-1 e1745ts mutant. 1 Publication1
Mutagenesisi87R → Q in ay73; partial rod-like larval lethality. Surviving mutant have a thin body and no vulva. Rescues fluid accumulation in clr-1 e1745ts mutant. 1 Publication1
Mutagenesisi90E → K in n1779; mild defect in vulva development and partially impaired sex myoblast migration. Causes ectopic muscle membrane extension. Reduced lev-1 synaptic levels. Moderate increase in resistance to nicotine-induced paralysis. Rescues fluid accumulation in crl-1 e1745ts mutant. 3 Publications1
Mutagenesisi91S → N in n1781; no obvious defects. Partially rescues fluid accumulation in crl-1 e1745ts mutant. 1 Publication1
Mutagenesisi201G → R in n2195; loss of interaction with soc-1. Partially restores normal vulva induction in let-60 n1046gf mutant. Partially rescues fluid accumulation in crl-1 e1745ts mutant. Partially prevents constitutive dauer formation in daf-2 m577 mutant. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882121 – 228Sex muscle abnormal protein 5Add BLAST228

Proteomic databases

EPDiP29355
PaxDbiP29355
PeptideAtlasiP29355
PRIDEiP29355

Expressioni

Tissue specificityi

Expressed in body wall muscles, pharynx, intestine and hypodermis.1 Publication

Gene expression databases

BgeeiWBGene00004774

Interactioni

Subunit structurei

Interacts (probably via SH3 domain 2) with soc-1 (via C-terminus) (PubMed:16547100). Interacts with mig-2 (active GTP-bound form) and wsp-1 (PubMed:27780040). Interacts with mig-13; the interaction is direct (PubMed:27780040).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Sos1Q622452EBI-315286,EBI-1693From Mus musculus.

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: WormBase
  • SH3/SH2 adaptor activity Source: WormBase

Protein-protein interaction databases

BioGridi45979, 113 interactors
DIPiDIP-27394N
IntActiP29355, 28 interactors
MINTiP29355
STRINGi6239.C14F5.5

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi158 – 163Combined sources6
Beta strandi169 – 172Combined sources4
Beta strandi180 – 185Combined sources6
Beta strandi187 – 196Combined sources10
Beta strandi199 – 204Combined sources6
Helixi205 – 207Combined sources3
Beta strandi208 – 212Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K76NMR-A155-214[»]
1KFZNMR-A155-214[»]
1SEMX-ray2.00A/B155-212[»]
2SEMX-ray2.20A/B155-214[»]
3SEMX-ray2.20A/B155-214[»]
ProteinModelPortaliP29355
SMRiP29355
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29355

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58SH3 1PROSITE-ProRule annotationAdd BLAST58
Domaini60 – 152SH2PROSITE-ProRule annotationAdd BLAST93
Domaini154 – 213SH3 2PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3601 Eukaryota
ENOG410XR1G LUCA
GeneTreeiENSGT00820000126999
HOGENOMiHOG000251625
InParanoidiP29355
KOiK04364
OMAiHWWHGEI
OrthoDBiEOG091G0HWS
PhylomeDBiP29355

Family and domain databases

Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR030219 Grb2
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR24418:SF290 PTHR24418:SF290, 1 hit
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 2 hits
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 2 hits
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 2 hits
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 2 hits

Sequencei

Sequence statusi: Complete.

P29355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVAEHDFQ AGSPDELSFK RGNTLKVLNK DEDPHWYKAE LDGNEGFIPS
60 70 80 90 100
NYIRMTECNW YLGKITRNDA EVLLKKPTVR DGHFLVRQCE SSPGEFSISV
110 120 130 140 150
RFQDSVQHFK VLRDQNGKYY LWAVKFNSLN ELVAYHRTAS VSRTHTILLS
160 170 180 190 200
DMNVETKFVQ ALFDFNPQES GELAFKRGDV ITLINKDDPN WWEGQLNNRR
210 220
GIFPSNYVCP YNSNKSNSNV APGFNFGN
Length:228
Mass (Da):26,210
Last modified:December 1, 1992 - v1
Checksum:i2D684C9520646C41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S88446 mRNA Translation: AAB21850.1
FO080456 Genomic DNA Translation: CCD63850.1
PIRiS25730
RefSeqiNP_509342.1, NM_076941.5
UniGeneiCel.19703

Genome annotation databases

EnsemblMetazoaiC14F5.5; C14F5.5; WBGene00004774
GeneIDi181055
KEGGicel:CELE_C14F5.5
UCSCiC14F5.5 c. elegans

Similar proteinsi

Entry informationi

Entry nameiSEM5_CAEEL
AccessioniPrimary (citable) accession number: P29355
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: March 28, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health