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P29352

- PTN22_MOUSE

UniProt

P29352 - PTN22_MOUSE

Protein

Tyrosine-protein phosphatase non-receptor type 22

Gene

Ptpn22

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-492' residue. Dephosphorylates the immune system activator SKAP2 By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei195 – 1951SubstrateBy similarity
    Active sitei227 – 2271Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei274 – 2741SubstrateBy similarity

    GO - Molecular functioni

    1. kinase binding Source: BHF-UCL
    2. protein binding Source: UniProtKB
    3. protein tyrosine phosphatase activity Source: UniProtKB
    4. SH3 domain binding Source: BHF-UCL

    GO - Biological processi

    1. negative regulation of T cell activation Source: BHF-UCL
    2. negative regulation of T cell receptor signaling pathway Source: UniProtKB
    3. peptidyl-tyrosine dephosphorylation Source: GOC
    4. phosphoanandamide dephosphorylation Source: BHF-UCL
    5. protein dephosphorylation Source: MGI
    6. regulation of natural killer cell proliferation Source: BHF-UCL
    7. T cell differentiation Source: MGI
    8. T cell receptor signaling pathway Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase non-receptor type 22 (EC:3.1.3.48)
    Alternative name(s):
    Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP
    PEST-domain phosphatase
    Short name:
    PEP
    Gene namesi
    Name:Ptpn22
    Synonyms:Ptpn8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:107170. Ptpn22.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytoplasmic side of plasma membrane Source: UniProtKB
    3. nucleus Source: UniProtKB
    4. perinuclear region of cytoplasm Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 802802Tyrosine-protein phosphatase non-receptor type 22PRO_0000094776Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei35 – 351Phosphoserine; by PKC/PRKCDBy similarity
    Modified residuei634 – 6341Phosphoserine1 Publication
    Modified residuei680 – 6801Phosphoserine1 Publication
    Modified residuei687 – 6871Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation on Ser-35 by PKC/PRKCD abrogates its ability to dephosphorylate and inactivate the SRC family kinases.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP29352.

    PTM databases

    PhosphoSiteiP29352.

    Expressioni

    Tissue specificityi

    Spleen, thymus, lymph node and bone marrow.

    Gene expression databases

    ArrayExpressiP29352.
    BgeeiP29352.
    GenevestigatoriP29352.

    Interactioni

    Subunit structurei

    Interacts with CBL By similarity. Interacts with CSK. Interacts with LPXN.By similarity3 Publications

    Protein-protein interaction databases

    IntActiP29352. 2 interactions.
    MINTiMINT-1535132.

    Structurei

    Secondary structure

    1
    802
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi621 – 6244

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JEGNMR-B605-629[»]
    ProteinModelPortaliP29352.
    SMRiP29352. Positions 3-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29352.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 289266Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni227 – 2337Substrate bindingBy similarity
    Regioni613 – 6219Interaction with CSK

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00750000117233.
    HOGENOMiHOG000252955.
    HOVERGENiHBG103877.
    InParanoidiP29352.
    KOiK18024.
    OMAiADEDCMQ.
    PhylomeDBiP29352.
    TreeFamiTF351977.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR016276. Non-rcpt_Tyr_Pase_8/22.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000930. PTPN8_PTPN22. 1 hit.
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29352-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQREILQQL LKEAQKKKLN SEEFASEFLK LKRQSTKYKA DKIYPTTVAQ    50
    RPKNIKKNRY KDILPYDHSL VELSLLTSDE DSSYINASFI KGVYGPKAYI 100
    ATQGPLSTTL LDFWRMIWEY RILVIVMACM EFEMGKKKCE RYWAEPGETQ 150
    LQFGPFSISC EAEKKKSDYK IRTLKAKFNN ETRIIYQFHY KNWPDHDVPS 200
    SIDPILQLIW DMRCYQEDDC VPICIHCSAG CGRTGVICAV DYTWMLLKDG 250
    IIPKNFSVFN LIQEMRTQRP SLVQTQEQYE LVYSAVLELF KRHMDVISDN 300
    HLGREIQAQC SIPEQSLTVE ADSCPLDLPK NAMRDVKTTN QHSKQGAEAE 350
    STGGSSLGLR TSTMNAEEEL VLHSAKSSPS FNCLELNCGC NNKAVITRNG 400
    QARASPVVGE PLQKYQSLDF GSMLFGSCPS ALPINTADRY HNSKGPVKRT 450
    KSTPFELIQQ RKTNDLAVGD GFSCLESQLH EHYSLRELQV QRVAHVSSEE 500
    LNYSLPGACD ASCVPRHSPG ALRVHLYTSL AEDPYFSSSP PNSADSKMSF 550
    DLPEKQDGAT SPGALLPASS TTSFFYSNPH DSLVMNTLTS FSPPLNQETA 600
    VEAPSRRTDD EIPPPLPERT PESFIVVEEA GEPSPRVTES LPLVVTFGAS 650
    PECSGTSEMK SHDSVGFTPS KNVKLRSPKS DRHQDGSPPP PLPERTLESF 700
    FLADEDCIQA QAVQTSSTSY PETTENSTSS KQTLRTPGKS FTRSKSLKIF 750
    RNMKKSVCNS SSPSKPTERV QPKNSSSFLN FGFGNRFSKP KGPRNPPSAW 800
    NM 802
    Length:802
    Mass (Da):89,714
    Last modified:December 1, 1992 - v1
    Checksum:i0F1E45339BD4613E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti91 – 911K → Q in AAH55377. (PubMed:15489334)Curated
    Sequence conflicti141 – 1411R → H in AAH55377. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90388 mRNA. Translation: AAA39994.1.
    BC055377 mRNA. Translation: AAH55377.1.
    CCDSiCCDS38577.1.
    PIRiB44390.
    RefSeqiNP_033005.1. NM_008979.2.
    XP_006501211.1. XM_006501148.1.
    XP_006501212.1. XM_006501149.1.
    XP_006501213.1. XM_006501150.1.
    UniGeneiMm.395.

    Genome annotation databases

    EnsembliENSMUST00000029433; ENSMUSP00000029433; ENSMUSG00000027843.
    GeneIDi19260.
    KEGGimmu:19260.
    UCSCiuc008qtv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90388 mRNA. Translation: AAA39994.1 .
    BC055377 mRNA. Translation: AAH55377.1 .
    CCDSi CCDS38577.1.
    PIRi B44390.
    RefSeqi NP_033005.1. NM_008979.2.
    XP_006501211.1. XM_006501148.1.
    XP_006501212.1. XM_006501149.1.
    XP_006501213.1. XM_006501150.1.
    UniGenei Mm.395.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JEG NMR - B 605-629 [» ]
    ProteinModelPortali P29352.
    SMRi P29352. Positions 3-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P29352. 2 interactions.
    MINTi MINT-1535132.

    Chemistry

    ChEMBLi CHEMBL2157855.

    PTM databases

    PhosphoSitei P29352.

    Proteomic databases

    PRIDEi P29352.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029433 ; ENSMUSP00000029433 ; ENSMUSG00000027843 .
    GeneIDi 19260.
    KEGGi mmu:19260.
    UCSCi uc008qtv.1. mouse.

    Organism-specific databases

    CTDi 26191.
    MGIi MGI:107170. Ptpn22.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00750000117233.
    HOGENOMi HOG000252955.
    HOVERGENi HBG103877.
    InParanoidi P29352.
    KOi K18024.
    OMAi ADEDCMQ.
    PhylomeDBi P29352.
    TreeFami TF351977.

    Miscellaneous databases

    ChiTaRSi PTPN22. mouse.
    EvolutionaryTracei P29352.
    NextBioi 296116.
    PROi P29352.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P29352.
    Bgeei P29352.
    Genevestigatori P29352.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR016276. Non-rcpt_Tyr_Pase_8/22.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000930. PTPN8_PTPN22. 1 hit.
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of hematopoietic intracellular protein tyrosine phosphatases: description of a phosphatase containing an SH2 domain and another enriched in proline-, glutamic acid-, serine-, and threonine-rich sequences."
      Matthews R.J., Bowne D.B., Flores E., Thomas M.L.
      Mol. Cell. Biol. 12:2396-2405(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6NCr.
      Tissue: Hematopoietic stem cell.
    3. "Association of inhibitory tyrosine protein kinase p50csk with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells."
      Cloutier J.-F., Veillette A.
      EMBO J. 15:4909-4918(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 495-789, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CSK.
      Tissue: Splenocyte.
    4. "Leupaxin binds to PEST domain tyrosine phosphatase PEP."
      Watanabe N., Amano N., Ishizuka H., Mashima K.
      Mol. Cell. Biochem. 269:13-17(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LPXN.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-634; SER-680 AND SER-687, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "A novel, specific interaction involving the Csk SH3 domain and its natural ligand."
      Ghose R., Shekhtman A., Goger M.J., Ji H., Cowburn D.
      Nat. Struct. Biol. 8:998-1004(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 612-629 IN COMPLEX WITH CSK.

    Entry informationi

    Entry nameiPTN22_MOUSE
    AccessioniPrimary (citable) accession number: P29352
    Secondary accession number(s): Q7TMP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3