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P29347 (MTS1_STRSA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase StsI

Short name=M.StsI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase StsI
Gene names
Name:stsIM
OrganismStreptococcus sanguinis
Taxonomic identifier1305 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence 5'-GGATG-3' in one strand and 3'-CATCC-5' in the other, causes specific methylation on the A of both strands, and protects the DNA from cleavage by the StsI endonuclease. The 2 domains of the protein participates in modification of the two strands.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Monomer.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 653653Modification methylase StsI
PRO_0000087962

Sequences

Sequence LengthMass (Da)Tools
P29347 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: C4D42B76E5665E91

FASTA65376,068
        10         20         30         40         50         60 
MRYIGSKKLL LPEIKKMVDK HTDGSEEVFL DLFAGTNVVA NYFKQFYTVY SNDMLFFSYV 

        70         80         90        100        110        120 
NAKATIENNS KPSFSKLIQA GISSPMTYLQ NLEVNDETIG YYEVAYSPTG EANYLSVHNA 

       130        140        150        160        170        180 
KKLDIIRSQI ESWKNQNLLT EHEYYYLLSS LIEALPFISN TTGTYGAFLK HWDKRSLNDL 

       190        200        210        220        230        240 
ELQDFTIFDN SKQNKAFNED ANELVQKIKA DIVYIDTPYN SRQYASNYHL LENVARNEHP 

       250        260        270        280        290        300 
TLKGITKIFD WKNLKSDYAT KGKALVAMRD LIQNINSTHI ILSYNNEGII SEEDLTNILK 

       310        320        330        340        350        360 
EFSVDGIVDI KKIPYRKYQS KNVSKNKEIY ELLFYIQRKP FSKNKTLNKP LNNVRVSSTK 

       370        380        390        400        410        420 
KYIKSPLNYI GGKYKLLNQI LPLFPKNINT FVDIFSGGAN VGINVKAKKY IFNDMNTRIN 

       430        440        450        460        470        480 
EMFRYFQTQP PVKLVQQIEE KIDEWGLSKT NEDAFLAFRK HYNTNPNPLD LYVLSSFSYN 

       490        500        510        520        530        540 
YQFRFNNSME FNNPFGRNRS HFSENMRNNL LNFVTKLQTL DATFTDNYFN EFDFSNLTSN 

       550        560        570        580        590        600 
DFIYLDPPYL ITTGSYNDGK RGFSDWNNTS EMKLLNFMDY LNQHGIRFAL SNVTEHKGKT 

       610        620        630        640        650 
NQLLKDWAYS RNLNVNYLDH NYNNSSHNSK SKGSQEVLIT NYETKTFNLL NTK 

« Hide

References

[1]"Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes."
Kita K., Suisha M., Kotani H., Yanase H., Kato N.
Nucleic Acids Res. 20:4167-4172(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 54.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D11101 Genomic DNA. Translation: BAA01876.1.
PIRS35493.

3D structure databases

ProteinModelPortalP29347.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3512. M.StsI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
InterProIPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
[Graphical view]
PfamPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFPIRSF036638. M_m6A_StsI. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTS1_STRSA
AccessionPrimary (citable) accession number: P29347
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: December 11, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries