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P29347

- MTS1_STRSA

UniProt

P29347 - MTS1_STRSA

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Protein

Modification methylase StsI

Gene

stsIM

Organism
Streptococcus sanguinis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence 5'-GGATG-3' in one strand and 3'-CATCC-5' in the other, causes specific methylation on the A of both strands, and protects the DNA from cleavage by the StsI endonuclease. The 2 domains of the protein participates in modification of the two strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3512. M.StsI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase StsI (EC:2.1.1.72)
Short name:
M.StsI
Alternative name(s):
Adenine-specific methyltransferase StsI
Gene namesi
Name:stsIM
OrganismiStreptococcus sanguinis
Taxonomic identifieri1305 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 653653Modification methylase StsIPRO_0000087962Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP29347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProiIPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFiPIRSF036638. M_m6A_StsI. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29347-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRYIGSKKLL LPEIKKMVDK HTDGSEEVFL DLFAGTNVVA NYFKQFYTVY
60 70 80 90 100
SNDMLFFSYV NAKATIENNS KPSFSKLIQA GISSPMTYLQ NLEVNDETIG
110 120 130 140 150
YYEVAYSPTG EANYLSVHNA KKLDIIRSQI ESWKNQNLLT EHEYYYLLSS
160 170 180 190 200
LIEALPFISN TTGTYGAFLK HWDKRSLNDL ELQDFTIFDN SKQNKAFNED
210 220 230 240 250
ANELVQKIKA DIVYIDTPYN SRQYASNYHL LENVARNEHP TLKGITKIFD
260 270 280 290 300
WKNLKSDYAT KGKALVAMRD LIQNINSTHI ILSYNNEGII SEEDLTNILK
310 320 330 340 350
EFSVDGIVDI KKIPYRKYQS KNVSKNKEIY ELLFYIQRKP FSKNKTLNKP
360 370 380 390 400
LNNVRVSSTK KYIKSPLNYI GGKYKLLNQI LPLFPKNINT FVDIFSGGAN
410 420 430 440 450
VGINVKAKKY IFNDMNTRIN EMFRYFQTQP PVKLVQQIEE KIDEWGLSKT
460 470 480 490 500
NEDAFLAFRK HYNTNPNPLD LYVLSSFSYN YQFRFNNSME FNNPFGRNRS
510 520 530 540 550
HFSENMRNNL LNFVTKLQTL DATFTDNYFN EFDFSNLTSN DFIYLDPPYL
560 570 580 590 600
ITTGSYNDGK RGFSDWNNTS EMKLLNFMDY LNQHGIRFAL SNVTEHKGKT
610 620 630 640 650
NQLLKDWAYS RNLNVNYLDH NYNNSSHNSK SKGSQEVLIT NYETKTFNLL

NTK
Length:653
Mass (Da):76,068
Last modified:December 1, 1992 - v1
Checksum:iC4D42B76E5665E91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11101 Genomic DNA. Translation: BAA01876.1.
PIRiS35493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11101 Genomic DNA. Translation: BAA01876.1 .
PIRi S35493.

3D structure databases

ProteinModelPortali P29347.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3512. M.StsI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProi IPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 2 hits.
[Graphical view ]
PIRSFi PIRSF036638. M_m6A_StsI. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes."
    Kita K., Suisha M., Kotani H., Yanase H., Kato N.
    Nucleic Acids Res. 20:4167-4172(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 54.

Entry informationi

Entry nameiMTS1_STRSA
AccessioniPrimary (citable) accession number: P29347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3