P29347 (MTS1_STRSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase StsI
Short name=M.StsI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase StsI

Gene names

Name:

stsIM

Organism

Streptococcus sanguis

Taxonomic identifier

1305 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence 5'-GGATG-3' in one strand and 3'-CATCC-5' in the other, causes specific methylation on the A of both strands, and protects the DNA from cleavage by the StsI endonuclease. The 2 domains of the protein participates in modification of the two strands.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 653	653	Modification methylase StsI		PRO_0000087962

Sequences

	Sequence		Length	Mass (Da)
	P29347 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: C4D42B76E5665E91	FASTA	653	76,068
	10 20 30 40 50 60 MRYIGSKKLL LPEIKKMVDK HTDGSEEVFL DLFAGTNVVA NYFKQFYTVY SNDMLFFSYV 70 80 90 100 110 120 NAKATIENNS KPSFSKLIQA GISSPMTYLQ NLEVNDETIG YYEVAYSPTG EANYLSVHNA 130 140 150 160 170 180 KKLDIIRSQI ESWKNQNLLT EHEYYYLLSS LIEALPFISN TTGTYGAFLK HWDKRSLNDL 190 200 210 220 230 240 ELQDFTIFDN SKQNKAFNED ANELVQKIKA DIVYIDTPYN SRQYASNYHL LENVARNEHP 250 260 270 280 290 300 TLKGITKIFD WKNLKSDYAT KGKALVAMRD LIQNINSTHI ILSYNNEGII SEEDLTNILK 310 320 330 340 350 360 EFSVDGIVDI KKIPYRKYQS KNVSKNKEIY ELLFYIQRKP FSKNKTLNKP LNNVRVSSTK 370 380 390 400 410 420 KYIKSPLNYI GGKYKLLNQI LPLFPKNINT FVDIFSGGAN VGINVKAKKY IFNDMNTRIN 430 440 450 460 470 480 EMFRYFQTQP PVKLVQQIEE KIDEWGLSKT NEDAFLAFRK HYNTNPNPLD LYVLSSFSYN 490 500 510 520 530 540 YQFRFNNSME FNNPFGRNRS HFSENMRNNL LNFVTKLQTL DATFTDNYFN EFDFSNLTSN 550 560 570 580 590 600 DFIYLDPPYL ITTGSYNDGK RGFSDWNNTS EMKLLNFMDY LNQHGIRFAL SNVTEHKGKT 610 620 630 640 650 NQLLKDWAYS RNLNVNYLDH NYNNSSHNSK SKGSQEVLIT NYETKTFNLL NTK

References

[1]

"Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes." Kita K., Suisha M., Kotani H., Yanase H., Kato N. Nucleic Acids Res. 20:4167-4172(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 54.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11101 Genomic DNA. Translation: BAA01876.1.
PIR	S35493.
3D structure databases
ProteinModelPortal	P29347.
ModBase	Search...
Protein family/group databases
REBASE	3512. M.StsI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1020.10. 1 hit.
InterPro	IPR012186. Ade-mod_methylase_MStsI. IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 2 hits. [Graphical view]
PIRSF	PIRSF036638. M_m6A_StsI. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTS1_STRSA

Accession

Primary (citable) accession number: P29347

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program