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P29346

- T2S1_STRSA

UniProt

P29346 - T2S1_STRSA

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Protein
Type-2 restriction enzyme StsI
Gene
stsIR
Organism
Streptococcus sanguinis
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequences GGATG and CATCC and cleaves respectively 15 bases after G-1 and 14 bases before C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1784. StsI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme StsI (EC:3.1.21.4)
Short name:
R.StsI
Alternative name(s):
Endonuclease StsI
Type II restriction enzyme StsI
Gene namesi
Name:stsIR
OrganismiStreptococcus sanguinis
Taxonomic identifieri1305 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 602601Type-2 restriction enzyme StsI
PRO_0000077368Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP29346.

Family & Domainsi

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.91.30. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR011578. Restrct_endonuc_C/endonuc_I.
IPR015334. Restrct_endonuc_II_FokI_C.
IPR004233. Restrct_endonuc_II_FokI_cat.
IPR004234. Restrct_endonuc_II_FokI_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09254. Endonuc-FokI_C. 1 hit.
PF02980. FokI_C. 1 hit.
PF02981. FokI_N. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29346-1 [UniParc]FASTAAdd to Basket

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MTISINEYSD LNNLAFGLGQ DVSQDLKELV KVASIFMPDS KIHKWLIDTR    50
LEEVVTDLNL RYELKSVITN TPISVTWKQL TGTRTKREAN SLVQAVFPGQ 100
CSRLAIVDWA AKNYVSVAVA FGLLKFHRAD KTFTISEIGI QAVKLYDSEE 150
LAELDKFLYE RLLEYPYAAW LIRLLGNQPS KQFSKFDLGE HFGFIDELGF 200
ETAPIEIFLN GLAQAEIDGD KTAAQKIKSN FESTSDKYMR WLAGVLVTAG 250
LATSTTKKVT HTYKNRKFEL TLGTVYQITA KGLTALKEVN GKSRYPRSRK 300
RVMWEFLATK DKEAIAKKTS RSLMLKHLTE KKNPIQAEVI ATLINTDYPT 350
LEITPEEVID DCIGLNRIGI EILIDGDKLT LNDKLFDFEI PVQKDVVLEK 400
SDIEKFKNQL RTELTNIDHS YLKGIDIASK KKTSNVENTE FEAISTKIFT 450
DELGFSGKHL GGSNKPDGLL WDDDCAIILD SKAYSEGFPL TASHTDAMGR 500
YLRQFTERKE EIKPTWWDIA PEHLDNTYFA YVSGSFSGNY KEQLQKFRQD 550
TNHLGGALEF VKLLLLANNY KTQKMSKKEV KKSILDYNIS YEEYAPLLAE 600
IE 602
Length:602
Mass (Da):68,392
Last modified:January 23, 2007 - v2
Checksum:i8FFCC465145F5B99
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11101 Genomic DNA. Translation: BAA01875.1.
PIRiS35495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11101 Genomic DNA. Translation: BAA01875.1 .
PIRi S35495.

3D structure databases

ProteinModelPortali P29346.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1784. StsI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.40.91.30. 1 hit.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR011578. Restrct_endonuc_C/endonuc_I.
IPR015334. Restrct_endonuc_II_FokI_C.
IPR004233. Restrct_endonuc_II_FokI_cat.
IPR004234. Restrct_endonuc_II_FokI_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF09254. Endonuc-FokI_C. 1 hit.
PF02980. FokI_C. 1 hit.
PF02981. FokI_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes."
    Kita K., Suisha M., Kotani H., Yanase H., Kato N.
    Nucleic Acids Res. 20:4167-4172(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-22.
    Strain: 54.

Entry informationi

Entry nameiT2S1_STRSA
AccessioniPrimary (citable) accession number: P29346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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