Reviewed, UniProtKB/Swiss-Prot P29346 (T2S1_STRSA)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme StsI
      Short name=R.StsI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme StsI
    Endonuclease StsI

Gene names

Name:

stsIR

Organism

Streptococcus sanguis

Taxonomic identifier

1305 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequences GGATG and CATCC and cleaves respectively 15 bases after G-1 and 14 bases before C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 602	601	Type-2 restriction enzyme StsI		PRO_0000077368

Sequences

	Sequence		Length	Mass (Da)
	P29346-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8FFCC465145F5B99	FASTA	602	68,392
	10 20 30 40 50 60 MTISINEYSD LNNLAFGLGQ DVSQDLKELV KVASIFMPDS KIHKWLIDTR LEEVVTDLNL 70 80 90 100 110 120 RYELKSVITN TPISVTWKQL TGTRTKREAN SLVQAVFPGQ CSRLAIVDWA AKNYVSVAVA 130 140 150 160 170 180 FGLLKFHRAD KTFTISEIGI QAVKLYDSEE LAELDKFLYE RLLEYPYAAW LIRLLGNQPS 190 200 210 220 230 240 KQFSKFDLGE HFGFIDELGF ETAPIEIFLN GLAQAEIDGD KTAAQKIKSN FESTSDKYMR 250 260 270 280 290 300 WLAGVLVTAG LATSTTKKVT HTYKNRKFEL TLGTVYQITA KGLTALKEVN GKSRYPRSRK 310 320 330 340 350 360 RVMWEFLATK DKEAIAKKTS RSLMLKHLTE KKNPIQAEVI ATLINTDYPT LEITPEEVID 370 380 390 400 410 420 DCIGLNRIGI EILIDGDKLT LNDKLFDFEI PVQKDVVLEK SDIEKFKNQL RTELTNIDHS 430 440 450 460 470 480 YLKGIDIASK KKTSNVENTE FEAISTKIFT DELGFSGKHL GGSNKPDGLL WDDDCAIILD 490 500 510 520 530 540 SKAYSEGFPL TASHTDAMGR YLRQFTERKE EIKPTWWDIA PEHLDNTYFA YVSGSFSGNY 550 560 570 580 590 600 KEQLQKFRQD TNHLGGALEF VKLLLLANNY KTQKMSKKEV KKSILDYNIS YEEYAPLLAE IE

References

[1]

"Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes." Kita K., Suisha M., Kotani H., Yanase H., Kato N. Nucleic Acids Res. 20:4167-4172(1992) [PubMed: 1387204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-22. Strain: 54.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D11101 Genomic DNA. Translation: BAA01875.1.
PIR	S35495.
3D structure databases
HSSP	HSSP built from PDB template 2FOK based on UniProtKB P14870.
ModBase	Search...
Protein family/group databases
REBASE	1784. StsI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 18606.
Family and domain databases
InterPro	IPR011578. Restrict_endonuc_C/endonuc_I. IPR015334. Restrict_endonuc_II_FokI_C. IPR004233. Restrict_endonuc_II_FokI_cat. IPR004234. Restrict_endonuc_II_FokI_N. [Graphical view]
Gene3D	G3DSA:3.40.91.30. Restrict_endonuc_C/endonuc_I. 1 hit.
Pfam	PF09254. Endonuc-FokI_C. 1 hit. PF02980. FokI_C. 1 hit. PF02981. FokI_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S1_STRSA

Accession

Primary (citable) accession number: P29346

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)