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P29346

- T2S1_STRSA

UniProt

P29346 - T2S1_STRSA

Protein

Type-2 restriction enzyme StsI

Gene

stsIR

Organism
Streptococcus sanguinis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequences GGATG and CATCC and cleaves respectively 15 bases after G-1 and 14 bases before C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1784. StsI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme StsI (EC:3.1.21.4)
    Short name:
    R.StsI
    Alternative name(s):
    Endonuclease StsI
    Type II restriction enzyme StsI
    Gene namesi
    Name:stsIR
    OrganismiStreptococcus sanguinis
    Taxonomic identifieri1305 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 602601Type-2 restriction enzyme StsIPRO_0000077368Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP29346.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.40.91.30. 1 hit.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR011578. Restrct_endonuc_C/endonuc_I.
    IPR015334. Restrct_endonuc_II_FokI_C.
    IPR004233. Restrct_endonuc_II_FokI_cat.
    IPR004234. Restrct_endonuc_II_FokI_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF09254. Endonuc-FokI_C. 1 hit.
    PF02980. FokI_C. 1 hit.
    PF02981. FokI_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29346-1 [UniParc]FASTAAdd to Basket

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    MTISINEYSD LNNLAFGLGQ DVSQDLKELV KVASIFMPDS KIHKWLIDTR    50
    LEEVVTDLNL RYELKSVITN TPISVTWKQL TGTRTKREAN SLVQAVFPGQ 100
    CSRLAIVDWA AKNYVSVAVA FGLLKFHRAD KTFTISEIGI QAVKLYDSEE 150
    LAELDKFLYE RLLEYPYAAW LIRLLGNQPS KQFSKFDLGE HFGFIDELGF 200
    ETAPIEIFLN GLAQAEIDGD KTAAQKIKSN FESTSDKYMR WLAGVLVTAG 250
    LATSTTKKVT HTYKNRKFEL TLGTVYQITA KGLTALKEVN GKSRYPRSRK 300
    RVMWEFLATK DKEAIAKKTS RSLMLKHLTE KKNPIQAEVI ATLINTDYPT 350
    LEITPEEVID DCIGLNRIGI EILIDGDKLT LNDKLFDFEI PVQKDVVLEK 400
    SDIEKFKNQL RTELTNIDHS YLKGIDIASK KKTSNVENTE FEAISTKIFT 450
    DELGFSGKHL GGSNKPDGLL WDDDCAIILD SKAYSEGFPL TASHTDAMGR 500
    YLRQFTERKE EIKPTWWDIA PEHLDNTYFA YVSGSFSGNY KEQLQKFRQD 550
    TNHLGGALEF VKLLLLANNY KTQKMSKKEV KKSILDYNIS YEEYAPLLAE 600
    IE 602
    Length:602
    Mass (Da):68,392
    Last modified:January 23, 2007 - v2
    Checksum:i8FFCC465145F5B99
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11101 Genomic DNA. Translation: BAA01875.1.
    PIRiS35495.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11101 Genomic DNA. Translation: BAA01875.1 .
    PIRi S35495.

    3D structure databases

    ProteinModelPortali P29346.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1784. StsI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.40.91.30. 1 hit.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR011578. Restrct_endonuc_C/endonuc_I.
    IPR015334. Restrct_endonuc_II_FokI_C.
    IPR004233. Restrct_endonuc_II_FokI_cat.
    IPR004234. Restrct_endonuc_II_FokI_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF09254. Endonuc-FokI_C. 1 hit.
    PF02980. FokI_C. 1 hit.
    PF02981. FokI_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes."
      Kita K., Suisha M., Kotani H., Yanase H., Kato N.
      Nucleic Acids Res. 20:4167-4172(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-22.
      Strain: 54.

    Entry informationi

    Entry nameiT2S1_STRSA
    AccessioniPrimary (citable) accession number: P29346
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3