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P29346 (T2S1_STRSA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme StsI

Short name=R.StsI
EC=3.1.21.4
Alternative name(s):
Endonuclease StsI
Type II restriction enzyme StsI
Gene names
Name:stsIR
OrganismStreptococcus sanguinis
Taxonomic identifier1305 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequences GGATG and CATCC and cleaves respectively 15 bases after G-1 and 14 bases before C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 602601Type-2 restriction enzyme StsI
PRO_0000077368

Sequences

Sequence LengthMass (Da)Tools
P29346 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 8FFCC465145F5B99

FASTA60268,392
        10         20         30         40         50         60 
MTISINEYSD LNNLAFGLGQ DVSQDLKELV KVASIFMPDS KIHKWLIDTR LEEVVTDLNL 

        70         80         90        100        110        120 
RYELKSVITN TPISVTWKQL TGTRTKREAN SLVQAVFPGQ CSRLAIVDWA AKNYVSVAVA 

       130        140        150        160        170        180 
FGLLKFHRAD KTFTISEIGI QAVKLYDSEE LAELDKFLYE RLLEYPYAAW LIRLLGNQPS 

       190        200        210        220        230        240 
KQFSKFDLGE HFGFIDELGF ETAPIEIFLN GLAQAEIDGD KTAAQKIKSN FESTSDKYMR 

       250        260        270        280        290        300 
WLAGVLVTAG LATSTTKKVT HTYKNRKFEL TLGTVYQITA KGLTALKEVN GKSRYPRSRK 

       310        320        330        340        350        360 
RVMWEFLATK DKEAIAKKTS RSLMLKHLTE KKNPIQAEVI ATLINTDYPT LEITPEEVID 

       370        380        390        400        410        420 
DCIGLNRIGI EILIDGDKLT LNDKLFDFEI PVQKDVVLEK SDIEKFKNQL RTELTNIDHS 

       430        440        450        460        470        480 
YLKGIDIASK KKTSNVENTE FEAISTKIFT DELGFSGKHL GGSNKPDGLL WDDDCAIILD 

       490        500        510        520        530        540 
SKAYSEGFPL TASHTDAMGR YLRQFTERKE EIKPTWWDIA PEHLDNTYFA YVSGSFSGNY 

       550        560        570        580        590        600 
KEQLQKFRQD TNHLGGALEF VKLLLLANNY KTQKMSKKEV KKSILDYNIS YEEYAPLLAE 


IE 

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References

[1]"Cloning and sequence analysis of the StsI restriction-modification gene: presence of homology to FokI restriction-modification enzymes."
Kita K., Suisha M., Kotani H., Yanase H., Kato N.
Nucleic Acids Res. 20:4167-4172(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-22.
Strain: 54.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D11101 Genomic DNA. Translation: BAA01875.1.
PIRS35495.

3D structure databases

ProteinModelPortalP29346.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1784. StsI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.10. 1 hit.
3.40.91.30. 1 hit.
InterProIPR011335. Restrct_endonuc-II-like.
IPR011578. Restrct_endonuc_C/endonuc_I.
IPR015334. Restrct_endonuc_II_FokI_C.
IPR004233. Restrct_endonuc_II_FokI_cat.
IPR004234. Restrct_endonuc_II_FokI_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF09254. Endonuc-FokI_C. 1 hit.
PF02980. FokI_C. 1 hit.
PF02981. FokI_N. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2S1_STRSA
AccessionPrimary (citable) accession number: P29346
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries