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P29340 (UBCX_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2-21 kDa

EC=6.3.2.19
Alternative name(s):
Peroxin-4
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene names
Name:PEX4
Synonyms:PAS2, UBC10
Ordered Locus Names:YGR133W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5. Ref.5

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Peroxisome.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PEX22P3971810EBI-19784,EBI-20707

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 183183Ubiquitin-conjugating enzyme E2-21 kDa
PRO_0000082558

Sites

Active site1151Glycyl thioester intermediate By similarity

Secondary structure

................................ 183
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29340 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: D4E438B689F76CAD

FASTA18321,118
        10         20         30         40         50         60 
MPNFWILENR RSYTSDTCMS RIVKEYKVIL KTLASDDPIA NPYRGIIESL NPIDETDLSK 

        70         80         90        100        110        120 
WEAIISGPSD TPYENHQFRI LIEVPSSYPM NPPKISFMQN NILHCNVKSA TGEICLNILK 

       130        140        150        160        170        180 
PEEWTPVWDL LHCVHAVWRL LREPVCDSPL DVDIGNIIRC GDMSAYQGIV KYFLAERERI 


NNH 

« Hide

References

« Hide 'large scale' references
[1]"The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymes."
Wiebel F.F., Kunau W.-H.
Nature 359:73-76(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p."
Williams C., van den Berg M., Sprenger R.R., Distel B.
J. Biol. Chem. 282:22534-22543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65470 Genomic DNA. Translation: CAA46467.1.
Z72918 Genomic DNA. Translation: CAA97146.1.
AY557776 Genomic DNA. Translation: AAS56102.1.
BK006941 Genomic DNA. Translation: DAA08226.1.
PIRS29088.
RefSeqNP_011649.1. NM_001181262.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y9MX-ray2.60A15-183[»]
2Y9PX-ray3.25A15-183[»]
4BWFX-ray3.23A15-183[»]
ProteinModelPortalP29340.
SMRP29340. Positions 15-179.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33381. 73 interactions.
DIPDIP-4411N.
IntActP29340. 4 interactions.
MINTMINT-566793.
STRING4932.YGR133W.

Protein family/group databases

TCDB3.A.20.1.1. the peroxisomal protein importer (ppi) family.
3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Proteomic databases

MaxQBP29340.
PaxDbP29340.
PeptideAtlasP29340.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR133W; YGR133W; YGR133W.
GeneID853034.
KEGGsce:YGR133W.

Organism-specific databases

CYGDYGR133w.
SGDS000003365. PEX4.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233455.
KOK10689.
OMACERMCIC.
OrthoDBEOG7JQC1C.

Enzyme and pathway databases

BioCycYEAST:G3O-30839-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP29340.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972929.

Entry information

Entry nameUBCX_YEAST
AccessionPrimary (citable) accession number: P29340
Secondary accession number(s): D6VUR5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways