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Protein

Ubiquitin-conjugating enzyme E2-21 kDa

Gene

PEX4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5.PROSITE-ProRule annotation1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • fatty acid beta-oxidation Source: EnsemblPlants
  • peroxisome organization Source: SGD
  • protein autoubiquitination Source: SGD
  • protein import into peroxisome matrix, receptor recycling Source: SGD
  • protein monoubiquitination Source: SGD
  • protein ubiquitination Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processPeroxisome biogenesis, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30839-MONOMER.
UniPathwayiUPA00143.

Protein family/group databases

TCDBi3.A.20.1.1. the peroxisomal protein importer (ppi) family.
3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-21 kDa (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme PEX4
Peroxin-4
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:PEX4
Synonyms:PAS2, UBC10
Ordered Locus Names:YGR133W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR133W.
SGDiS000003365. PEX4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825581 – 183Ubiquitin-conjugating enzyme E2-21 kDaAdd BLAST183

Proteomic databases

MaxQBiP29340.
PRIDEiP29340.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX22P3971810EBI-19784,EBI-20707

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33381. 184 interactors.
DIPiDIP-4411N.
IntActiP29340. 4 interactors.
MINTiMINT-566793.
STRINGi4932.YGR133W.

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 33Combined sources18
Turni38 – 40Combined sources3
Turni42 – 46Combined sources5
Beta strandi47 – 54Combined sources8
Beta strandi60 – 66Combined sources7
Beta strandi69 – 71Combined sources3
Turni72 – 75Combined sources4
Beta strandi77 – 83Combined sources7
Turni86 – 90Combined sources5
Beta strandi94 – 97Combined sources4
Turni109 – 111Combined sources3
Helixi117 – 119Combined sources3
Turni121 – 123Combined sources3
Helixi130 – 142Combined sources13
Helixi152 – 158Combined sources7
Turni159 – 161Combined sources3
Helixi163 – 178Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y9MX-ray2.60A15-183[»]
2Y9PX-ray3.25A15-183[»]
4BWFX-ray3.23A15-183[»]
ProteinModelPortaliP29340.
SMRiP29340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233455.
InParanoidiP29340.
KOiK10689.
OMAiFRILIEV.
OrthoDBiEOG092C50OL.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P29340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNFWILENR RSYTSDTCMS RIVKEYKVIL KTLASDDPIA NPYRGIIESL
60 70 80 90 100
NPIDETDLSK WEAIISGPSD TPYENHQFRI LIEVPSSYPM NPPKISFMQN
110 120 130 140 150
NILHCNVKSA TGEICLNILK PEEWTPVWDL LHCVHAVWRL LREPVCDSPL
160 170 180
DVDIGNIIRC GDMSAYQGIV KYFLAERERI NNH
Length:183
Mass (Da):21,118
Last modified:December 1, 1992 - v1
Checksum:iD4E438B689F76CAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65470 Genomic DNA. Translation: CAA46467.1.
Z72918 Genomic DNA. Translation: CAA97146.1.
AY557776 Genomic DNA. Translation: AAS56102.1.
BK006941 Genomic DNA. Translation: DAA08226.1.
PIRiS29088.
RefSeqiNP_011649.1. NM_001181262.1.

Genome annotation databases

EnsemblFungiiYGR133W; YGR133W; YGR133W.
GeneIDi853034.
KEGGisce:YGR133W.

Similar proteinsi

Entry informationi

Entry nameiUBCX_YEAST
AccessioniPrimary (citable) accession number: P29340
Secondary accession number(s): D6VUR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 22, 2017
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names