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P29340

- UBCX_YEAST

UniProt

P29340 - UBCX_YEAST

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Protein

Ubiquitin-conjugating enzyme E2-21 kDa

Gene
PEX4, PAS2, UBC10, YGR133W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. peroxisome organization Source: SGD
  2. protein autoubiquitination Source: SGD
  3. protein import into peroxisome matrix, receptor recycling Source: SGD
  4. protein monoubiquitination Source: SGD
  5. protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Peroxisome biogenesis, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30839-MONOMER.
UniPathwayiUPA00143.

Protein family/group databases

TCDBi3.A.20.1.1. the peroxisomal protein importer (ppi) family.
3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-21 kDa (EC:6.3.2.19)
Alternative name(s):
Peroxin-4
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:PEX4
Synonyms:PAS2, UBC10
Ordered Locus Names:YGR133W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR133w.
SGDiS000003365. PEX4.

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Ubiquitin-conjugating enzyme E2-21 kDaPRO_0000082558Add
BLAST

Proteomic databases

MaxQBiP29340.
PaxDbiP29340.
PeptideAtlasiP29340.

Expressioni

Gene expression databases

GenevestigatoriP29340.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX22P3971810EBI-19784,EBI-20707

Protein-protein interaction databases

BioGridi33381. 73 interactions.
DIPiDIP-4411N.
IntActiP29340. 4 interactions.
MINTiMINT-566793.
STRINGi4932.YGR133W.

Structurei

Secondary structure

1
183
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 3318
Turni38 – 403
Turni42 – 465
Beta strandi47 – 548
Beta strandi60 – 667
Beta strandi69 – 713
Turni72 – 754
Beta strandi77 – 837
Turni86 – 905
Beta strandi94 – 974
Turni109 – 1113
Helixi117 – 1193
Turni121 – 1233
Helixi130 – 14213
Helixi152 – 1587
Turni159 – 1613
Helixi163 – 17816

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y9MX-ray2.60A15-183[»]
2Y9PX-ray3.25A15-183[»]
4BWFX-ray3.23A15-183[»]
ProteinModelPortaliP29340.
SMRiP29340. Positions 15-179.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
KOiK10689.
OMAiCERMCIC.
OrthoDBiEOG7JQC1C.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29340-1 [UniParc]FASTAAdd to Basket

« Hide

MPNFWILENR RSYTSDTCMS RIVKEYKVIL KTLASDDPIA NPYRGIIESL    50
NPIDETDLSK WEAIISGPSD TPYENHQFRI LIEVPSSYPM NPPKISFMQN 100
NILHCNVKSA TGEICLNILK PEEWTPVWDL LHCVHAVWRL LREPVCDSPL 150
DVDIGNIIRC GDMSAYQGIV KYFLAERERI NNH 183
Length:183
Mass (Da):21,118
Last modified:December 1, 1992 - v1
Checksum:iD4E438B689F76CAD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65470 Genomic DNA. Translation: CAA46467.1.
Z72918 Genomic DNA. Translation: CAA97146.1.
AY557776 Genomic DNA. Translation: AAS56102.1.
BK006941 Genomic DNA. Translation: DAA08226.1.
PIRiS29088.
RefSeqiNP_011649.1. NM_001181262.1.

Genome annotation databases

EnsemblFungiiYGR133W; YGR133W; YGR133W.
GeneIDi853034.
KEGGisce:YGR133W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65470 Genomic DNA. Translation: CAA46467.1 .
Z72918 Genomic DNA. Translation: CAA97146.1 .
AY557776 Genomic DNA. Translation: AAS56102.1 .
BK006941 Genomic DNA. Translation: DAA08226.1 .
PIRi S29088.
RefSeqi NP_011649.1. NM_001181262.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2Y9M X-ray 2.60 A 15-183 [» ]
2Y9P X-ray 3.25 A 15-183 [» ]
4BWF X-ray 3.23 A 15-183 [» ]
ProteinModelPortali P29340.
SMRi P29340. Positions 15-179.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33381. 73 interactions.
DIPi DIP-4411N.
IntActi P29340. 4 interactions.
MINTi MINT-566793.
STRINGi 4932.YGR133W.

Protein family/group databases

TCDBi 3.A.20.1.1. the peroxisomal protein importer (ppi) family.
3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Proteomic databases

MaxQBi P29340.
PaxDbi P29340.
PeptideAtlasi P29340.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR133W ; YGR133W ; YGR133W .
GeneIDi 853034.
KEGGi sce:YGR133W.

Organism-specific databases

CYGDi YGR133w.
SGDi S000003365. PEX4.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
KOi K10689.
OMAi CERMCIC.
OrthoDBi EOG7JQC1C.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-30839-MONOMER.

Miscellaneous databases

NextBioi 972929.

Gene expression databases

Genevestigatori P29340.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymes."
    Wiebel F.F., Kunau W.-H.
    Nature 359:73-76(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p."
    Williams C., van den Berg M., Sprenger R.R., Distel B.
    J. Biol. Chem. 282:22534-22543(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiUBCX_YEAST
AccessioniPrimary (citable) accession number: P29340
Secondary accession number(s): D6VUR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 3, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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