Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P29340

- UBCX_YEAST

UniProt

P29340 - UBCX_YEAST

Protein

Ubiquitin-conjugating enzyme E2-21 kDa

Gene

PEX4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5.1 PublicationPROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei115 – 1151Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. peroxisome organization Source: SGD
    2. protein autoubiquitination Source: SGD
    3. protein import into peroxisome matrix, receptor recycling Source: SGD
    4. protein monoubiquitination Source: SGD
    5. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Peroxisome biogenesis, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30839-MONOMER.
    UniPathwayiUPA00143.

    Protein family/group databases

    TCDBi3.A.20.1.1. the peroxisomal protein importer (ppi) family.
    3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2-21 kDa (EC:6.3.2.19)
    Alternative name(s):
    Peroxin-4
    Ubiquitin carrier protein
    Ubiquitin-protein ligase
    Gene namesi
    Name:PEX4
    Synonyms:PAS2, UBC10
    Ordered Locus Names:YGR133W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR133w.
    SGDiS000003365. PEX4.

    Subcellular locationi

    GO - Cellular componenti

    1. peroxisome Source: SGD

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183Ubiquitin-conjugating enzyme E2-21 kDaPRO_0000082558Add
    BLAST

    Proteomic databases

    MaxQBiP29340.
    PaxDbiP29340.
    PeptideAtlasiP29340.

    Expressioni

    Gene expression databases

    GenevestigatoriP29340.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PEX22P3971810EBI-19784,EBI-20707

    Protein-protein interaction databases

    BioGridi33381. 72 interactions.
    DIPiDIP-4411N.
    IntActiP29340. 4 interactions.
    MINTiMINT-566793.
    STRINGi4932.YGR133W.

    Structurei

    Secondary structure

    1
    183
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi16 – 3318
    Turni38 – 403
    Turni42 – 465
    Beta strandi47 – 548
    Beta strandi60 – 667
    Beta strandi69 – 713
    Turni72 – 754
    Beta strandi77 – 837
    Turni86 – 905
    Beta strandi94 – 974
    Turni109 – 1113
    Helixi117 – 1193
    Turni121 – 1233
    Helixi130 – 14213
    Helixi152 – 1587
    Turni159 – 1613
    Helixi163 – 17816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Y9MX-ray2.60A15-183[»]
    2Y9PX-ray3.25A15-183[»]
    4BWFX-ray3.23A15-183[»]
    ProteinModelPortaliP29340.
    SMRiP29340. Positions 15-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    KOiK10689.
    OMAiCERMCIC.
    OrthoDBiEOG7JQC1C.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29340-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPNFWILENR RSYTSDTCMS RIVKEYKVIL KTLASDDPIA NPYRGIIESL    50
    NPIDETDLSK WEAIISGPSD TPYENHQFRI LIEVPSSYPM NPPKISFMQN 100
    NILHCNVKSA TGEICLNILK PEEWTPVWDL LHCVHAVWRL LREPVCDSPL 150
    DVDIGNIIRC GDMSAYQGIV KYFLAERERI NNH 183
    Length:183
    Mass (Da):21,118
    Last modified:December 1, 1992 - v1
    Checksum:iD4E438B689F76CAD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65470 Genomic DNA. Translation: CAA46467.1.
    Z72918 Genomic DNA. Translation: CAA97146.1.
    AY557776 Genomic DNA. Translation: AAS56102.1.
    BK006941 Genomic DNA. Translation: DAA08226.1.
    PIRiS29088.
    RefSeqiNP_011649.1. NM_001181262.1.

    Genome annotation databases

    EnsemblFungiiYGR133W; YGR133W; YGR133W.
    GeneIDi853034.
    KEGGisce:YGR133W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65470 Genomic DNA. Translation: CAA46467.1 .
    Z72918 Genomic DNA. Translation: CAA97146.1 .
    AY557776 Genomic DNA. Translation: AAS56102.1 .
    BK006941 Genomic DNA. Translation: DAA08226.1 .
    PIRi S29088.
    RefSeqi NP_011649.1. NM_001181262.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Y9M X-ray 2.60 A 15-183 [» ]
    2Y9P X-ray 3.25 A 15-183 [» ]
    4BWF X-ray 3.23 A 15-183 [» ]
    ProteinModelPortali P29340.
    SMRi P29340. Positions 15-179.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33381. 72 interactions.
    DIPi DIP-4411N.
    IntActi P29340. 4 interactions.
    MINTi MINT-566793.
    STRINGi 4932.YGR133W.

    Protein family/group databases

    TCDBi 3.A.20.1.1. the peroxisomal protein importer (ppi) family.
    3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Proteomic databases

    MaxQBi P29340.
    PaxDbi P29340.
    PeptideAtlasi P29340.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR133W ; YGR133W ; YGR133W .
    GeneIDi 853034.
    KEGGi sce:YGR133W.

    Organism-specific databases

    CYGDi YGR133w.
    SGDi S000003365. PEX4.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    KOi K10689.
    OMAi CERMCIC.
    OrthoDBi EOG7JQC1C.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-30839-MONOMER.

    Miscellaneous databases

    NextBioi 972929.

    Gene expression databases

    Genevestigatori P29340.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymes."
      Wiebel F.F., Kunau W.-H.
      Nature 359:73-76(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p."
      Williams C., van den Berg M., Sprenger R.R., Distel B.
      J. Biol. Chem. 282:22534-22543(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiUBCX_YEAST
    AccessioniPrimary (citable) accession number: P29340
    Secondary accession number(s): D6VUR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3