Reviewed, UniProtKB/Swiss-Prot P29339 (RIP2_MOMBA)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein momordin II     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Momordica balsamina (Bitter gourd) (Balsam apple)
Taxonomic identifier	3672 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Momordica

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 286	263	Ribosome-inactivating protein momordin II		PRO_0000030772
Sites
	Active site	181	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29339-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 3B89FF1AE6B25986	FASTA	286	32,032
	10 20 30 40 50 60 MVKCLLLSFL IIAIFIGVPT AKGDVNFDLS TATAKTYTKF IEDFRATLPF SHKVYDIPLL 70 80 90 100 110 120 YSTISDSRRF ILLDLTSYAY ETISVAIDVT NVYVVAYRTR DVSYFFKESP PEAYNILFKG 130 140 150 160 170 180 TRKITLPYTG NYENLQTAAH KIRENIDLGL PALSSAITTL FYYNAQSAPS ALLVLIQTTA 190 200 210 220 230 240 EAARFKYIER HVAKYVATNF KPNLAIISLE NQWSALSKQI FLAQNQGGKF RNPVDLIKPT 250 260 270 280 GERFQVTNVD SDVVKGNIKL LLNSRASTAD ENFITTMTLL GESVVN

References

[1]	"Momordin II, a ribosome inactivating protein from Momordica balsamina, is homologous to other plant proteins." Ortigao M., Better M. Nucleic Acids Res. 20:4662-4662(1992) [PubMed: 1408771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z12175 Genomic DNA. Translation: CAA78166.1.
PIR	S25560.
3D structure databases
SMR	P29339. Positions 24-286.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 295560.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP2_MOMBA

Accession

Primary (citable) accession number: P29339

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)