P29336 (GTFS_STRDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucosyltransferase-S
Short name=GTF-S
EC=2.4.1.5

Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase

Gene names

Name:

gtfS

Organism

Streptococcus downei (Streptococcus sobrinus)

Taxonomic identifier

1317 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
Catalytic activity	Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).
Enzyme regulation	Glucan synthesis by GTF-S is independent of primer glucan unlike GTF-I.
Miscellaneous	Synthesizes water-soluble glucans (alpha 1,6-glucose).
Sequence similarities	Belongs to the glycosyl hydrolase 70 family. Contains 12 cell wall-binding repeats.

Ontologies

Keywords
Disease	Dental caries
Domain	Repeat Signal
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological_process	glucan biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dextransucrase activity Inferred from electronic annotation. Source: EC glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Or 37 Potential
	Chain	37 – 1365	1329	Glucosyltransferase-S		PRO_0000021388
Regions
	Repeat	146 – 166	21	Cell wall-binding 1
	Repeat	168 – 187	20	Cell wall-binding 2
	Repeat	1052 – 1071	20	Cell wall-binding 3
	Repeat	1073 – 1092	20	Cell wall-binding 4
	Repeat	1093 – 1112	20	Cell wall-binding 5
	Repeat	1113 – 1133	21	Cell wall-binding 6
	Repeat	1136 – 1159	24	Cell wall-binding 7
	Repeat	1160 – 1179	20	Cell wall-binding 8
	Repeat	1234 – 1253	20	Cell wall-binding 9
	Repeat	1278 – 1298	21	Cell wall-binding 10
	Repeat	1299 – 1318	20	Cell wall-binding 11
	Repeat	1343 – 1362	20	Cell wall-binding 12
	Region	200 – 1000	801	Catalytic; approximate

Sequences

	Sequence		Length	Mass (Da)
	P29336 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 167296B5A2E8C476	FASTA	1,365	151,591
	10 20 30 40 50 60 MEKNLRYKLH KVKKQWVAIG VTTVTLSFLA GGQVVAADTN NNDGTSVQVN KMVPSDPKFD 70 80 90 100 110 120 AQAQNGQLAQ AMFKAANQAD QTATSQVSPA TDGRVDNQVT PAANQPAANV ANQDVANPAT 130 140 150 160 170 180 DAGALNRQSA ADTSTDGKAV PQTSDQPGHL ETVDGKTYYV DANGQRLKNY SMVIDGKTYY 190 200 210 220 230 240 FDGQTGEAQT DLPKTGQANQ DNVPDSYQAN NQAYSNEASS FETVDNYLTA DSWYRPRKIL 250 260 270 280 290 300 KNGQSWQASS EGDLRPILMT WWPDAATKAA YANFWAKEGL ISGSYRQNSA NLDAATQNIQ 310 320 330 340 350 360 SAIEKKIASE GNTNWLRDKM SQFVKSQNQW SIASENETVY PNQDHMQGGA LLFSNSKDTE 370 380 390 400 410 420 HANSDWRLLN RNPTFQTGKQ KYFTTNYAGY ELLLANDVDN SNPVVQAEQL NHLHYLMNWG 430 440 450 460 470 480 DIVMGDKDAN FDGVRVDAVD NVNADLLQIQ RDYYKAKYGT DQNEKNAIDH LSILEAWSGN 490 500 510 520 530 540 DNDYVKDQNN FSLSIDNDQR SGMLKAFGYA SAYRGNLSNL ATAGLKNRSA NPDSDPVPNY 550 560 570 580 590 600 VFIRAHDSEV QTRIAKIIRE KLGKTNADGL TNLTLDDLNK AFDIYNQDMN ATDKVYYPNN 610 620 630 640 650 660 LPMAYAWMLQ NKDTVTRVYY GDMYTDNGQY MATKTPFYNA IETLLKGRIK YVAGGQAVSY 670 680 690 700 710 720 KQDWSSGILT SVRYGKGANS ASDAGNTETR NSGMALLINN RPNFRAYRNL TLNMGAAHKS 730 740 750 760 770 780 QAYRPLLLST KDGIATYLND SDVDSRQYKY TDSQGNLSFS ASELQSVANA QVSGMIQVWV 790 800 810 820 830 840 PVGAADNQDV RTSPSTQATK DGNIYHQSDA LDSQVIYEGF SNFQAFAQSP DQYTNAVIAK 850 860 870 880 890 900 NGDLFKSWGI TQFEMAPQYV SSEDGTFLDS VILNGYAFSD RYDLAMSKNN KYGSKQDLAN 910 920 930 940 950 960 AIKGLQSAGI KVLSDLVPNQ LYNLPGKEVV TATRVNQYGQ AKSGATINKT PYVANTRSYG 970 980 990 1000 1010 1020 DYQEQYGGKF LDDLQKLYPR LFSTKQISTG KPIDPSVKIT NWSAKYFNGS NILGRGAKYV 1030 1040 1050 1060 1070 1080 LSEGNKYLNL ADGKLFLPTV LNNTYGQPQV SANGFISKNG GIHYLDKNGQ EVKNRFKEIS 1090 1100 1110 1120 1130 1140 GSWYYFDSDG KMATGKTKIG NDTYLFMPNG KQLKEGVWYD GKKAYYYDDN GRTWTNKGFV 1150 1160 1170 1180 1190 1200 EFRVDGQDKW RYFNGDGTIA IGLVSLDNRT LYFDAYGYQV KGQTVTINGK SYTFDADQGD 1210 1220 1230 1240 1250 1260 LVQTDNANPA PQGQAGWKLL GDNQWGYRKD GQLLTGEQTI DGQKVFFQDN GVQVKGGTAT 1270 1280 1290 1300 1310 1320 DASGVLRFYD RDQGHQVGKG WYSTSDDNWV YVNESGQVLT GLQTIDGQTV YFDDKGIQAK 1330 1340 1350 1360 GKAVWDENGN LRYFDADSGN MLRDRWKNVD GNWYYFNRNG LATRW

References

[1]	"Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans." Gilmore K.S., Russell R.R., Ferretti J.J. Infect. Immun. 58:2452-2458(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MFE28.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30943 Genomic DNA. Translation: AAA26898.1.
3D structure databases
ProteinModelPortal	P29336.
ModBase	Search...
Protein family/group databases
CAZy	GH70. Glycoside Hydrolase Family 70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 2 hits.
InterPro	IPR018337. Cell_wall/Cho-bd_repeat. IPR003318. Glyco_hydro70cat. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR022263. KxYKxGKxW. [Graphical view]
Pfam	PF01473. CW_binding_1. 2 hits. PF02324. Glyco_hydro_70. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 2 hits.
TIGRFAMs	TIGR03715. KxYKxGKxW. 1 hit.
PROSITE	PS51170. CW. 12 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GTFS_STRDO

Accession

Primary (citable) accession number: P29336

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program