Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P29336 (GTFS_STRDO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosyltransferase-S

Short name=GTF-S
EC=2.4.1.5
Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase
Gene names
Name:gtfS
OrganismStreptococcus downei (Streptococcus sobrinus)
Taxonomic identifier1317 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length1365 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activity

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

Enzyme regulation

Glucan synthesis by GTF-S is independent of primer glucan unlike GTF-I.

Miscellaneous

Synthesizes water-soluble glucans (alpha 1,6-glucose).

Sequence similarities

Belongs to the glycosyl hydrolase 70 family.

Contains 12 cell wall-binding repeats.

Ontologies

Keywords
   DiseaseDental caries
   DomainRepeat
Signal
   Molecular functionGlycosyltransferase
Transferase
Gene Ontology (GO)
   Biological_processglucan biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functiondextransucrase activity

Inferred from electronic annotation. Source: UniProtKB-EC

glucosyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3636Or 37 Potential
Chain37 – 13651329Glucosyltransferase-S
PRO_0000021388

Regions

Repeat146 – 16621Cell wall-binding 1
Repeat168 – 18720Cell wall-binding 2
Repeat1052 – 107120Cell wall-binding 3
Repeat1073 – 109220Cell wall-binding 4
Repeat1093 – 111220Cell wall-binding 5
Repeat1113 – 113321Cell wall-binding 6
Repeat1136 – 115924Cell wall-binding 7
Repeat1160 – 117920Cell wall-binding 8
Repeat1234 – 125320Cell wall-binding 9
Repeat1278 – 129821Cell wall-binding 10
Repeat1299 – 131820Cell wall-binding 11
Repeat1343 – 136220Cell wall-binding 12
Region200 – 1000801Catalytic; approximate

Sequences

Sequence LengthMass (Da)Tools
P29336 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 167296B5A2E8C476

FASTA1,365151,591
        10         20         30         40         50         60 
MEKNLRYKLH KVKKQWVAIG VTTVTLSFLA GGQVVAADTN NNDGTSVQVN KMVPSDPKFD 

        70         80         90        100        110        120 
AQAQNGQLAQ AMFKAANQAD QTATSQVSPA TDGRVDNQVT PAANQPAANV ANQDVANPAT 

       130        140        150        160        170        180 
DAGALNRQSA ADTSTDGKAV PQTSDQPGHL ETVDGKTYYV DANGQRLKNY SMVIDGKTYY 

       190        200        210        220        230        240 
FDGQTGEAQT DLPKTGQANQ DNVPDSYQAN NQAYSNEASS FETVDNYLTA DSWYRPRKIL 

       250        260        270        280        290        300 
KNGQSWQASS EGDLRPILMT WWPDAATKAA YANFWAKEGL ISGSYRQNSA NLDAATQNIQ 

       310        320        330        340        350        360 
SAIEKKIASE GNTNWLRDKM SQFVKSQNQW SIASENETVY PNQDHMQGGA LLFSNSKDTE 

       370        380        390        400        410        420 
HANSDWRLLN RNPTFQTGKQ KYFTTNYAGY ELLLANDVDN SNPVVQAEQL NHLHYLMNWG 

       430        440        450        460        470        480 
DIVMGDKDAN FDGVRVDAVD NVNADLLQIQ RDYYKAKYGT DQNEKNAIDH LSILEAWSGN 

       490        500        510        520        530        540 
DNDYVKDQNN FSLSIDNDQR SGMLKAFGYA SAYRGNLSNL ATAGLKNRSA NPDSDPVPNY 

       550        560        570        580        590        600 
VFIRAHDSEV QTRIAKIIRE KLGKTNADGL TNLTLDDLNK AFDIYNQDMN ATDKVYYPNN 

       610        620        630        640        650        660 
LPMAYAWMLQ NKDTVTRVYY GDMYTDNGQY MATKTPFYNA IETLLKGRIK YVAGGQAVSY 

       670        680        690        700        710        720 
KQDWSSGILT SVRYGKGANS ASDAGNTETR NSGMALLINN RPNFRAYRNL TLNMGAAHKS 

       730        740        750        760        770        780 
QAYRPLLLST KDGIATYLND SDVDSRQYKY TDSQGNLSFS ASELQSVANA QVSGMIQVWV 

       790        800        810        820        830        840 
PVGAADNQDV RTSPSTQATK DGNIYHQSDA LDSQVIYEGF SNFQAFAQSP DQYTNAVIAK 

       850        860        870        880        890        900 
NGDLFKSWGI TQFEMAPQYV SSEDGTFLDS VILNGYAFSD RYDLAMSKNN KYGSKQDLAN 

       910        920        930        940        950        960 
AIKGLQSAGI KVLSDLVPNQ LYNLPGKEVV TATRVNQYGQ AKSGATINKT PYVANTRSYG 

       970        980        990       1000       1010       1020 
DYQEQYGGKF LDDLQKLYPR LFSTKQISTG KPIDPSVKIT NWSAKYFNGS NILGRGAKYV 

      1030       1040       1050       1060       1070       1080 
LSEGNKYLNL ADGKLFLPTV LNNTYGQPQV SANGFISKNG GIHYLDKNGQ EVKNRFKEIS 

      1090       1100       1110       1120       1130       1140 
GSWYYFDSDG KMATGKTKIG NDTYLFMPNG KQLKEGVWYD GKKAYYYDDN GRTWTNKGFV 

      1150       1160       1170       1180       1190       1200 
EFRVDGQDKW RYFNGDGTIA IGLVSLDNRT LYFDAYGYQV KGQTVTINGK SYTFDADQGD 

      1210       1220       1230       1240       1250       1260 
LVQTDNANPA PQGQAGWKLL GDNQWGYRKD GQLLTGEQTI DGQKVFFQDN GVQVKGGTAT 

      1270       1280       1290       1300       1310       1320 
DASGVLRFYD RDQGHQVGKG WYSTSDDNWV YVNESGQVLT GLQTIDGQTV YFDDKGIQAK 

      1330       1340       1350       1360 
GKAVWDENGN LRYFDADSGN MLRDRWKNVD GNWYYFNRNG LATRW 

« Hide

References

[1]"Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans."
Gilmore K.S., Russell R.R., Ferretti J.J.
Infect. Immun. 58:2452-2458(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MFE28.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30943 Genomic DNA. Translation: AAA26898.1.

3D structure databases

ProteinModelPortalP29336.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 2 hits.
InterProIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamPF01473. CW_binding_1. 2 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 3 hits.
TIGRFAMsTIGR04035. glucan_65_rpt. 4 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEPS51170. CW. 12 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGTFS_STRDO
AccessionPrimary (citable) accession number: P29336
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 16, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries