Reviewed, UniProtKB/Swiss-Prot P29334 (LUXE_PHOLE)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--luciferin-component ligase
    EC=6.2.1.19
Alternative name(s):
    Acyl-protein synthetase

Gene names

Name:

luxE

Organism

Photobacterium leiognathi

Taxonomic identifier

658 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acyl-protein synthetase activates tetradecanoic acid. It is a component of the fatty acid reductase complex responsible for converting tetradecanoic acid to the aldehyde which serves as substrate in the luciferase-catalyzed reaction.
Catalytic activity	ATP + an acid + protein = AMP + diphosphate + an acyl-protein thioester.
Pathway	Lipid metabolism; bioluminescent fatty acid reduction.
Sequence similarities	Belongs to the luxE family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-luciferin-component ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Long-chain-fatty-acid--luciferin-component ligase		PRO_0000208063

Sequences

	Sequence		Length	Mass (Da)
	P29334-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 1B31848B00BF9921	FASTA	373	43,099
	10 20 30 40 50 60 MSTLLNIDAT EIKVSTEIDD IIFTSSPLTL LFEDQEKIQK ELILESFHYH YNHNKDYKYY 70 80 90 100 110 120 CNIQGVDENI QSIDDIPVFP TSMFKYSRLH TADESNIENW FTSSGTKGVK SHIARDRQSI 130 140 150 160 170 180 ERLLGSVNYG MKYLGEFHEH QLELVNMGPD RFSASNVWFK YVMSLVQLLY PTTFTVENDE 190 200 210 220 230 240 IDFEQTILAL KAIQRKGKGI CLIGPPYFIY LLCHYMKEHN IEFNAGAHMF IITGGGWKTK 250 260 270 280 290 300 QKEALNRQDF NQLLMETFSL FHESQIRDIF NQVELNTCFF EDSLQRKHVP PWVYARALDP 310 320 330 340 350 360 VTLTPVEDGQ EGLMSYMDAS STSYPTFIVT DDIGIVRHLK EPDPFQGTTV EIVRRLNTRE 370 QKGCSLSMAT SLK

References

[1]

"The lux genes of the luminous bacterial symbiont, Photobacterium leiognathi, of the ponyfish. Nucleotide sequence, difference in gene organization, and high expression in mutant Escherichia coli." Lee C.Y., Szittner R.B., Meighen E.A. Eur. J. Biochem. 201:161-167(1991) [PubMed: 1915359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25521 / L1 / CIP 665.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63594 Genomic DNA. Translation: AAA25620.1.
PIR	S17955.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.2.1.19. 349.
Family and domain databases
InterPro	IPR016671. Acyl-protein_synth_LuxE_bac. IPR007534. LuxE. [Graphical view]
Pfam	PF04443. LuxE. 1 hit. [Graphical view]
PIRSF	PIRSF016580. Acyl-protein_synthetase_LuxE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LUXE_PHOLE

Accession

Primary (citable) accession number: P29334

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)