Reviewed, UniProtKB/Swiss-Prot P29321 (EPHA8_RAT)

Names and origin

Protein names

Recommended name:
    Ephrin type-A receptor 8
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor EEK
    EPH- and ELK-related kinase

Gene names

Name:	Epha8
Synonyms:	Eek

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	372 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-A family.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with FYN By similarity. Interacts with ANKS1B By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Most abundant in brain.
Post-translational modification	Phosphorylation on Tyr-206 modulates tyrosine kinase activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane receptor protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 372	›372	Ephrin type-A receptor 8		PRO_0000160273
Regions
	Domain	2 – 263	262	Protein kinase
	Domain	297 – 372	76	SAM
	Nucleotide binding	8 – 16	9	ATP By similarity
	Motif	370 – 372	3	PDZ-binding Potential
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	34	1	ATP By similarity
Amino acid modifications
	Modified residue	206	1	Phosphotyrosine; by autocatalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P29321-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: C8FDCFB5A6904BA5	FASTA	372	41,233
	10 20 30 40 50 60 RIHIEKIIGS GESGEVCYGR LQVPGQRDVP VAIKALKAGY TERQRQDFLR EAAIMGQFDH 70 80 90 100 110 120 PNIIRLEGVV TRGRLAMIVT EYMENGSLDA FLRTHDGQFT ILQLVGMLKG VGAGMRYLSD 130 140 150 160 170 180 LGYIHRDLAA RNILVDGRLV CKVSDFGLSR ALEDDPEAAY TTAGGKIPIR WTAPEAIAFR 190 200 210 220 230 240 TFSSASDVWS FGVVMWEVLA YGERPYWNMT NQDVISSVEE GYRLPAPMGC PRALHQLMLD 250 260 270 280 290 300 CWHKDRAQRP RFSHVVSVLE ALVHSPESLR ATATVSRCPA PAFARSCFDL RAGGNGNGDL 310 320 330 340 350 360 TVGDWLDSIR MGRYRDHFAA GGYSSLGMVL HMNAQDVRAL GITLMGHQKK ILGSIQTMRS 370 QLSCTQGPRR HL

References

[1]	"eek and erk, new members of the eph subclass of receptor protein-tyrosine kinases." Chan J., Watt V.M. Oncogene 6:1057-1061(1991) [PubMed: 1648701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59290 mRNA. Translation: CAA41979.1.
IPI	IPI00778705.
PIR	S23363.
UniGene	Rn.62934
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
SMR	P29321. Positions 293-367.
ModBase	Search...
Protein-protein interaction databases
STRING	P29321.
PTM databases
PhosphoSite	P29321.
Genome annotation databases
Ensembl	ENSRNOT00000017559; ENSRNOP00000017559; ENSRNOG00000013036; Rattus norvegicus. [Genome view] ENSRNOT00000055987; ENSRNOP00000052841; ENSRNOG00000013036; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	708543. Epha8.
Phylogenomic databases
HOVERGEN	P29321.
Enzyme and pathway databases
BRENDA	2.7.10.1. 248.
Gene expression databases
ArrayExpress	P29321.
Genevestigator	P29321.
GermOnline	ENSRNOG00000013036. Rattus norvegicus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR013761. SAM_type. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR001426. YKase_receptorV_CS. [Graphical view]
Gene3D	G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. Partial match. PS00791. RECEPTOR_TYR_KIN_V_2. Partial match. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPHA8_RAT

Accession

Primary (citable) accession number: P29321

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	October 13, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)