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Reviewed, UniProtKB/Swiss-Prot P29319 (EPHA3_MOUSE)

Last modified October 13, 2009. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ephrin type-A receptor 3
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor ETK1
    MEK4
    Tyrosine-protein kinase TYRO4
Gene names
Name: Epha3
Synonyms: Etk1, Mek4, Tyro4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length983 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Isoform Long: Cell membrane; Single-pass type I membrane protein.

Isoform Short: Secreted.

Tissue specificity

Greatest levels of expression occurring in the brain, also detected in testis.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Contains 2 fibronectin type-III domains.

Contains 1 protein kinase domain.

Contains 1 SAM (sterile alpha motif) domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P29319-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P29319-2)

The sequence of this isoform differs from the canonical sequence as follows:
     530-983: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 By similarity
Chain21 – 983963Ephrin type-A receptor 3
PRO_0000016803

Regions

Topological domain21 – 540520Extracellular Potential
Transmembrane541 – 56424 Potential
Topological domain565 – 983419Cytoplasmic Potential
Domain324 – 428105Fibronectin type-III 1
Domain436 – 52792Fibronectin type-III 2
Domain621 – 882262Protein kinase
Domain911 – 97565SAM
Nucleotide binding627 – 6359ATP By similarity
Motif981 – 9833PDZ-binding Potential
Compositional bias188 – 321134Cys-rich

Sites

Active site7461Proton acceptor By similarity
Binding site6531ATP By similarity

Amino acid modifications

Modified residue5961Phosphotyrosine; by autocatalysis Potential
Modified residue6021Phosphotyrosine; by autocatalysis Potential
Modified residue7791Phosphotyrosine; by autocatalysis By similarity
Modified residue9371Phosphotyrosine Ref.2
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential
Glycosylation3901N-linked (GlcNAc...) Potential
Glycosylation4031N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence530 – 983454Missing in isoform Short.
VSP_002997

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Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: BE44A6655D8107A2

FASTA983109,955
        10         20         30         40         50         60 
MDCHLSILVL LGCCVLSCSG ELSPQPSNEV NLLDSKTIQG ELGWISYPSH GWEEISGVDE 

        70         80         90        100        110        120 
HYTPIRTYQV CNVMDHSQNN WLRTNWVPRN SAQKIYVELK FTLRDCNSIP LVLGTCKETF 

       130        140        150        160        170        180 
NLYYMESDDH GVKFREHQFT KIDTIAADES FTQMDLGDRI LKLNTEIREV GPVNKKGFYL 

       190        200        210        220        230        240 
AFQDVGACVA LVSVRVYFKK CPFTVKNLAM FPDTVPMDSQ SLVEVRGSCV NNSKEEDPPR 

       250        260        270        280        290        300 
MYCSTEGEWL VPIGKCTCNA GYEERGFICQ ACRPGFYKAS DGAAKCAKCP PHSSTQEDGS 

       310        320        330        340        350        360 
MNCRCENNYF RAEKDPPSMA CARPPSAPRN VISNINETSV ILDWSWPLDT GGRKDITFNI 

       370        380        390        400        410        420 
ICKKCGWNVR QCEPCSPNVR FLPRQLGLTN TTVTVTDLLA HTNYTFEIDA VNGVSELSSP 

       430        440        450        460        470        480 
PRQYAAVSIT TNQAAPSPVM TIKKDRTSRN SISLSWQEPE HPNGIILDYE VKYYQKQEQE 

       490        500        510        520        530        540 
TSYTILRARG TNVTISSLKP DTTYVFQIRA RTAAGYGTNS RKFEFETSPD SFSISGENSH 

       550        560        570        580        590        600 
VVMIAISAAV AIIVLTVVTY VLVGRFCGYH KSKHSAEEKR LHFGNGHLKL PGLRTYVDPH 

       610        620        630        640        650        660 
TYEDPTQAVH EFAKELDATN ISIDKVVGAG EFGEVCSGRL KLPSKKEISV AIKTLKVGYT 

       670        680        690        700        710        720 
EKQRRDFLGE ASIMGQFDHP NIIRLEGVVT KSKPEMIVTE YMENGSLDSF LRKHDAQFTV 

       730        740        750        760        770        780 
IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR NILINSNLVC KVSDFGLSRV LEDDPEAAYT 

       790        800        810        820        830        840 
TRGGKIPIRW TSPEAMSYRK FTSASDVWSY GIVLWEVMSY GERPYSQMSN QDVIKAVDER 

       850        860        870        880        890        900 
YRLPPPMDCP AALYQLMLDC WQKDRNNRPK FEQIVSILDK LIRNPGSLKI ITSAAARPSN 

       910        920        930        940        950        960 
LLLDQSNVDI ATFHTTGDWL NGMRTAHCKE IFTGVEYSSC DTIAKISTDD MKKVGVTVVG 

       970        980 
PQKKIISTIK ALETQSKNGP VPV

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Isoform Short.

Checksum: 4C08B176131F36CC
Show »

FASTA52959,456

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References

« Hide 'large scale' references
[1]"Identification of a new eph-related receptor tyrosine kinase gene from mouse and chicken that is developmentally regulated and encodes at least two forms of the receptor."
Sajjadi F.G., Pasquale E.B., Subramani S.
New Biol. 3:769-778(1991) [PubMed: 1657122] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Strain: ICR X Swiss Webster.
Tissue: Embryo.
[2]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-937, MASS SPECTROMETRY.
Tissue: Mast cell.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M68513 mRNA. Translation: AAA39521.1.
M68515 mRNA. Translation: AAA39522.1. Sequence problems.
IPIIPI00124182.
IPI00223194.
PIRA45583.
UniGeneMm.1977

3D structure databases

HSSPHSSP built from PDB template 1JPA based on UniProtKB P54763.
SMRP29319. Positions 601-899.
ModBaseSearch...

Protein-protein interaction databases

STRINGP29319.

PTM databases

PhosphoSiteP29319.

Proteomic databases

PRIDEP29319.

Genome annotation databases

EnsemblENSMUST00000064405; ENSMUSP00000066554; ENSMUSG00000052504; Mus musculus. [Genome view]

Organism-specific databases

MGIMGI:99612. Epha3.

Phylogenomic databases

HOVERGENP29319.

Gene expression databases

ArrayExpressP29319.
BgeeP29319.
CleanExMM_EPHA3.
GenevestigatorP29319.
GermOnlineENSMUSG00000052504. Mus musculus.

Family and domain databases

InterProIPR013032. EGF-like_reg_CS.
IPR001090. Ephrin_rcpt_lig-bd.
IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR003962. FnIII_subd.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR011510. SAM_2.
IPR013761. SAM_type.
IPR001245. Tyr_pkinase.
IPR008266. Tyr_pkinase_AS.
IPR016257. TyrPK_ephrin_receptor.
IPR001426. YKase_receptorV_CS.
[Graphical view]
Gene3DG3DSA:2.60.40.30. FN_III-like. 2 hits.
G3DSA:1.10.150.50. SAM_type. 1 hit.
PfamPF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PIRSFPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSPR00014. FNTYPEIII.
PR00109. TYRKINASE.
ProDomPD001495. Ephrin_receptor. 1 hit.
PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
PROSITEPS01186. EGF_2. 1 hit. Uncertain.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameEPHA3_MOUSE
AccessionPrimary (citable) accession number: P29319
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 13, 2009
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents