Reviewed, UniProtKB/Swiss-Prot P29318 (EPHA3_CHICK)

Names and origin

Protein names

Recommended name:
    Ephrin type-A receptor 3
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor ETK1
    CEK4

Gene names

Name:	EPHA3
Synonyms:	CEK4, ETK1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	983 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Highly expressed in the developing brain and embryonic tissues. In adult, the greatest levels of expression occur in the brain. It is expressed in a graded manner across the retina with the highest expression at its temporal pole. Detectable in all other adult tissues examined, except the liver.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 983	964	Ephrin type-A receptor 3		PRO_0000016805
Regions
	Topological domain	20 – 540	521	Extracellular Potential
	Transmembrane	541 – 564	24	Potential
	Topological domain	565 – 983	419	Cytoplasmic Potential
	Domain	324 – 426	103	Fibronectin type-III 1
	Domain	432 – 527	96	Fibronectin type-III 2
	Domain	621 – 882	262	Protein kinase
	Domain	911 – 975	65	SAM
	Nucleotide binding	627 – 635	9	ATP By similarity
	Motif	981 – 983	3	PDZ-binding Potential
	Compositional bias	188 – 321	134	Cys-rich
Sites
	Active site	746	1	Proton acceptor By similarity
	Binding site	653	1	ATP By similarity
Amino acid modifications
	Modified residue	596	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	602	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	779	1	Phosphotyrosine; by autocatalysis Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential
	Glycosylation	492	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P29318-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: E8895F0BDF77651E	FASTA	983	109,910
	10 20 30 40 50 60 MDRRRLPLLL LCAALGSAGR LSARPGNEVN LLDSKTIQGE LGWISYPSHG WEEISGVDEH 70 80 90 100 110 120 YTPIRTYQES NVMDHSQNNW LRTNWIPRNS AQKIYVELKF TLRDCNSIPL VLGTCKETFN 130 140 150 160 170 180 LYYMESDDDH LAKFREHQFT KIDTIAADES FTQMDLGDRI LKLNTEVREV GPVSKKGFYL 190 200 210 220 230 240 AFQDVGACVA LVSVRVYFKK CPFTVKNLAM FPDTVPMDSQ SLVEVRGSCV NHSKEEEPPK 250 260 270 280 290 300 MYCSTEGEWL VPIGKCLCNA GYEERGFACQ ACRPGFYKAS AGNVKCAKCP PHSSTYEDAS 310 320 330 340 350 360 LNCRCEKNYF RSEKDPPSMA CTRPPSAPRN VISNINETSV ILDWSWPLDT GGRKDVTFNI 370 380 390 400 410 420 ICKKCGGSSK ICEPCSDNVR FLPRQTGLTN TTVTVVDLLA HTNYTFEIDA VNGVSDLSTL 430 440 450 460 470 480 SRQFAAVSIT TNQAAPSPIT VIRKDRTSRN SVSLSWQEPE HPNGIILDYE VKYYEKQEQE 490 500 510 520 530 540 TSYTILRAKS TNVTISGLKP DTTYVFQIRA RTAARYGTSS RKFEFETSPD SFSISSENSQ 550 560 570 580 590 600 VVMIAISAAV AIILLTVVVY VLIGRFCGYK KSKHGTDEKR LHFGNGHLKL PGLRTYVDPH 610 620 630 640 650 660 TYEDPNQAVH EFAKELDASN ISIDKVVGAG EFGEVCSGRL KLPSKKEISV AIKTLKAGYT 670 680 690 700 710 720 EKQRRDFLGE ASIMGQFDHP NIIRLEGVVT KSKPVMIVTE YMENGSLDSF LRKHDAQFTV 730 740 750 760 770 780 IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR NILINSNLVC KVSDFGLSRV LEDDPEAAYT 790 800 810 820 830 840 TRGGKIPIRW TSPEAIAYRK FTSASDAWSY GIVLWEVMSY GERPYWEMSF QDVIKAVDEG 850 860 870 880 890 900 YRLPPPMDCP AALYQLMLDC WQKDRNNRPK FEQIVSILDK LIRNPSSLKI ITNAAARPSN 910 920 930 940 950 960 LLLDQSNIDI SAFRTAGDWL NGFRTGQCKG IFTGVEYSSC DTIAKISTDD MKKVGVTVVG 970 980 PQKKIVSSIK TLETHTKNSP VPV

References

[1]

"Identification of a new eph-related receptor tyrosine kinase gene from mouse and chicken that is developmentally regulated and encodes at least two forms of the receptor." Sajjadi F.G., Pasquale E.B., Subramani S. New Biol. 3:769-778(1991) [PubMed: 1657122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M68514 mRNA. Translation: AAA48666.1.
IPI	IPI00591501.
PIR	B45583.
RefSeq	NP_990761.1.
UniGene	Gga.805
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
SMR	P29318. Positions 601-899.
ModBase	Search...
Proteomic databases
PRIDE	P29318.
Genome annotation databases
Ensembl	ENSGALT00000024844; ENSGALP00000024798; ENSGALG00000015403; Gallus gallus. [Genome view]
GeneID	396402.
KEGG	gga:396402.
Organism-specific databases
CTD	396402.
Phylogenomic databases
HOGENOM	P29318.
HOVERGEN	P29318.
Enzyme and pathway databases
BRENDA	2.7.10.1. 4.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR013761. SAM_type. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016257. TyrPK_ephrin_receptor. IPR001426. YKase_receptorV_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07714. Pkinase_Tyr. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII. PR00109. TYRKINASE.
ProDom	PD001495. Ephrin_receptor. 1 hit. PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPHA3_CHICK

Accession

Primary (citable) accession number: P29318

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	October 13, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)