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Protein

Ephrin type-A receptor 3

Gene

EPHA3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei653 – 6531ATPPROSITE-ProRule annotation
Active sitei746 – 7461Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi628 – 6336ATPPROSITE-ProRule annotation
Nucleotide bindingi700 – 7067ATPPROSITE-ProRule annotation
Nucleotide bindingi750 – 7512ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-A receptor 3 (EC:2.7.10.1)
Alternative name(s):
EPH-like kinase 4
Short name:
EK4
Short name:
cEK4
Tyrosine-protein kinase receptor ETK1
Gene namesi
Name:EPHA3
Synonyms:CEK4, ETK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 540521ExtracellularSequence analysisAdd
BLAST
Transmembranei541 – 56424HelicalSequence analysisAdd
BLAST
Topological domaini565 – 983419CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 983964Ephrin type-A receptor 3PRO_0000016805Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence analysis
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence analysis
Modified residuei596 – 5961Phosphotyrosine; by autocatalysisBy similarity
Modified residuei602 – 6021Phosphotyrosine; by autocatalysisBy similarity
Modified residuei701 – 7011Phosphotyrosine; by autocatalysisBy similarity
Modified residuei779 – 7791Phosphotyrosine; by autocatalysisSequence analysis

Post-translational modificationi

Autophosphorylates upon activation by EFNA5.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP29318.
PRIDEiP29318.

Expressioni

Tissue specificityi

Highly expressed in the developing brain and embryonic tissues. In adult, the greatest levels of expression occur in the brain. It is expressed in a graded manner across the retina with the highest expression at its temporal pole. Detectable in all other adult tissues examined, except the liver.

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).By similarity

Protein-protein interaction databases

IntActiP29318. 1 interaction.
STRINGi9031.ENSGALP00000024798.

Structurei

3D structure databases

ProteinModelPortaliP29318.
SMRiP29318. Positions 28-202, 613-906.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 206179Eph LBDPROSITE-ProRule annotationAdd
BLAST
Domaini324 – 434111Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini435 – 53096Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini621 – 882262Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini911 – 97565SAMPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi981 – 9833PDZ-bindingSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi188 – 321134Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
InParanoidiP29318.
KOiK05104.
PhylomeDBiP29318.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF07699. Ephrin_rec_like. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS01186. EGF_2. 1 hit.
PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRRRLPLLL LCAALGSAGR LSARPGNEVN LLDSKTIQGE LGWISYPSHG
60 70 80 90 100
WEEISGVDEH YTPIRTYQES NVMDHSQNNW LRTNWIPRNS AQKIYVELKF
110 120 130 140 150
TLRDCNSIPL VLGTCKETFN LYYMESDDDH LAKFREHQFT KIDTIAADES
160 170 180 190 200
FTQMDLGDRI LKLNTEVREV GPVSKKGFYL AFQDVGACVA LVSVRVYFKK
210 220 230 240 250
CPFTVKNLAM FPDTVPMDSQ SLVEVRGSCV NHSKEEEPPK MYCSTEGEWL
260 270 280 290 300
VPIGKCLCNA GYEERGFACQ ACRPGFYKAS AGNVKCAKCP PHSSTYEDAS
310 320 330 340 350
LNCRCEKNYF RSEKDPPSMA CTRPPSAPRN VISNINETSV ILDWSWPLDT
360 370 380 390 400
GGRKDVTFNI ICKKCGGSSK ICEPCSDNVR FLPRQTGLTN TTVTVVDLLA
410 420 430 440 450
HTNYTFEIDA VNGVSDLSTL SRQFAAVSIT TNQAAPSPIT VIRKDRTSRN
460 470 480 490 500
SVSLSWQEPE HPNGIILDYE VKYYEKQEQE TSYTILRAKS TNVTISGLKP
510 520 530 540 550
DTTYVFQIRA RTAARYGTSS RKFEFETSPD SFSISSENSQ VVMIAISAAV
560 570 580 590 600
AIILLTVVVY VLIGRFCGYK KSKHGTDEKR LHFGNGHLKL PGLRTYVDPH
610 620 630 640 650
TYEDPNQAVH EFAKELDASN ISIDKVVGAG EFGEVCSGRL KLPSKKEISV
660 670 680 690 700
AIKTLKAGYT EKQRRDFLGE ASIMGQFDHP NIIRLEGVVT KSKPVMIVTE
710 720 730 740 750
YMENGSLDSF LRKHDAQFTV IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR
760 770 780 790 800
NILINSNLVC KVSDFGLSRV LEDDPEAAYT TRGGKIPIRW TSPEAIAYRK
810 820 830 840 850
FTSASDAWSY GIVLWEVMSY GERPYWEMSF QDVIKAVDEG YRLPPPMDCP
860 870 880 890 900
AALYQLMLDC WQKDRNNRPK FEQIVSILDK LIRNPSSLKI ITNAAARPSN
910 920 930 940 950
LLLDQSNIDI SAFRTAGDWL NGFRTGQCKG IFTGVEYSSC DTIAKISTDD
960 970 980
MKKVGVTVVG PQKKIVSSIK TLETHTKNSP VPV
Length:983
Mass (Da):109,910
Last modified:December 1, 1992 - v1
Checksum:iE8895F0BDF77651E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68514 mRNA. Translation: AAA48666.1.
PIRiB45583.
RefSeqiNP_990761.1. NM_205430.2.
UniGeneiGga.805.

Genome annotation databases

GeneIDi396402.
KEGGigga:396402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68514 mRNA. Translation: AAA48666.1.
PIRiB45583.
RefSeqiNP_990761.1. NM_205430.2.
UniGeneiGga.805.

3D structure databases

ProteinModelPortaliP29318.
SMRiP29318. Positions 28-202, 613-906.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP29318. 1 interaction.
STRINGi9031.ENSGALP00000024798.

Proteomic databases

PaxDbiP29318.
PRIDEiP29318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396402.
KEGGigga:396402.

Organism-specific databases

CTDi2042.

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
InParanoidiP29318.
KOiK05104.
PhylomeDBiP29318.

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.

Miscellaneous databases

PROiP29318.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF07699. Ephrin_rec_like. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS01186. EGF_2. 1 hit.
PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPHA3_CHICK
AccessioniPrimary (citable) accession number: P29318
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 8, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.