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Protein

14-3-3 protein zeta

Gene

14-3-3zeta

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required in Raf-dependent cell proliferation and photoreceptor differentiation during eye development (PubMed:9159395). Acts upstream of Raf and downstream of Ras, and is essential for viability (PubMed:9159395). Acts as a negative regulator of the slo calcium channel via its interaction with slo-binding protein slob (PubMed:10230800). Inhibits yki activity by restricting its nuclear localization (PubMed:19900439). Binds to and promotes the activity of phosphoinositide 3-kinase Pi3K68D which converts phosphatidylinositol to phosphatidylinositol-3-phosphate and promotes maturation of early endosomes (PubMed:27015288).4 Publications

GO - Molecular functioni

  • protein domain specific binding Source: InterPro
  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase
  • protein kinase C inhibitor activity Source: FlyBase

GO - Biological processi

  • chromosome segregation Source: FlyBase
  • germarium-derived oocyte fate determination Source: FlyBase
  • learning or memory Source: FlyBase
  • mitotic cell cycle, embryonic Source: FlyBase
  • nervous system development Source: UniProtKB-KW
  • olfactory learning Source: FlyBase
  • oocyte microtubule cytoskeleton polarization Source: FlyBase
  • positive regulation of ERK1 and ERK2 cascade Source: FlyBase
  • positive regulation of Ras protein signal transduction Source: FlyBase
  • protein folding Source: FlyBase
  • protein stabilization Source: FlyBase
  • thermosensory behavior Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-DME-1445148 Translocation of GLUT4 to the plasma membrane
R-DME-2028269 Signaling by Hippo
R-DME-3769402 Deactivation of the beta-catenin transactivating complex
R-DME-390098 Phosphorylation-dependent inhibition of YKI
R-DME-392517 Rap1 signalling
R-DME-430116 GP1b-IX-V activation signalling
R-DME-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-DME-450604 KSRP (KHSRP) binds and destabilizes mRNA
R-DME-5625740 RHO GTPases activate PKNs
R-DME-5628897 TP53 Regulates Metabolic Genes
R-DME-5673000 RAF activation
R-DME-5674135 MAP2K and MAPK activation
R-DME-5675221 Negative regulation of MAPK pathway
R-DME-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-DME-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
SignaLinkiP29310

Protein family/group databases

TCDBi8.A.98.1.1 the 14-3-3 protein (14-3-3) family

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein zeta
Alternative name(s):
14-3-3-like protein
Protein Leonardo
Gene namesi
Name:14-3-3zeta
Synonyms:14-3-3, 14-3-3EZ, leo, THAP
ORF Names:CG17870
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0004907 14-3-3zeta

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59R → A: Abolishes homodimerization but not regulatory function on Slo; when associated with A-63. 1 Publication1
Mutagenesisi63R → A: Abolishes homodimerization but not regulatory function on Slo; when associated with A-59. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586511 – 24814-3-3 protein zetaAdd BLAST248

Proteomic databases

PaxDbiP29310
PRIDEiP29310

Expressioni

Tissue specificityi

Predominantly expressed in the ventral nerve cord of the embryo, and in the neural tissues of the head. Also found in the region posterior to the morphogenetic furrow of the eye imaginal disk where cells differentiate as photoreceptors.

Developmental stagei

Expressed throughout all stages of embryonic and larval development.

Gene expression databases

BgeeiFBgn0004907
ExpressionAtlasiP29310 differential
GenevisibleiP29310 DM

Interactioni

Subunit structurei

Homodimer; homodimerization is not essential for modulating the activity of Slo (PubMed:12529354). Interacts with phosphorylated Slob; the interaction with Slob mediates an indirect interaction with Slo (PubMed:10230800). Interacts with phosphorylated yki (PubMed:18256197, PubMed:19900439). Interacts with hemo; this represses 14-3-3zeta activity which prevents the 14-3-3zeta-mediated activation of phosphoinositide 3-kinase Pi3K68D. This, in turn, inhibits the Pi3K68D-mediated conversion of phosphatidylinositol to phosphatidylinositol-3-phosphate and prevents progression of early endosomes through the maturation process which regulates subsequent steps of phagocytic processing (PubMed:27015288). Interacts with REPTOR (when phosphorylated), this interaction may assist the cytoplasmic retention of REPTOR (PubMed:25920570).6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: InterPro
  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi61887, 88 interactors
DIPiDIP-17371N
IntActiP29310, 53 interactors
STRINGi7227.FBpp0087500

Structurei

3D structure databases

ProteinModelPortaliP29310
SMRiP29310
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
InParanoidiP29310
KOiK16197
OMAiLWTSSDS
OrthoDBiEOG091G0VKY
PhylomeDBiP29310

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform VI (identifier: P29310-1) [UniParc]FASTAAdd to basket
Also known as: D, J

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTVDKEELV QKAKLAEQSE RYDDMAQAMK SVTETGVELS NEERNLLSVA
60 70 80 90 100
YKNVVGARRS SWRVISSIEQ KTEASARKQQ LAREYRERVE KELREICYEV
110 120 130 140 150
LGLLDKYLIP KASNPESKVF YLKMKGDYYR YLAEVATGDA RNTVVDDSQT
160 170 180 190 200
AYQDAFDISK GKMQPTHPIR LGLALNFSVF YYEILNSPDK ACQLAKQAFD
210 220 230 240
DAIAELDTLN EDSYKDSTLI MQLLRDNLTL WTSDTQGDEA EPQEGGDN
Length:248
Mass (Da):28,228
Last modified:December 1, 1992 - v1
Checksum:iC645CF3B10C6701A
GO
Isoform VI' (identifier: P29310-2) [UniParc]FASTAAdd to basket
Also known as: A, G, H, I

The sequence of this isoform differs from the canonical sequence as follows:
     149-161: QTAYQDAFDISKG → KNAYQEAFDIAKT

Show »
Length:248
Mass (Da):28,283
Checksum:i0A1C1005D62C9699
GO
Isoform C (identifier: P29310-3) [UniParc]FASTAAdd to basket
Also known as: F

The sequence of this isoform differs from the canonical sequence as follows:
     146-161: DDSQTAYQDAFDISKG → EDSKKAYQEAFDIAKT
     185-193: LNSPDKACQ → INSPARACH

Note: No experimental confirmation available.Curated
Show »
Length:248
Mass (Da):28,304
Checksum:i307C55638427FB57
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform C (identifier: P29310-3)
Sequence conflicti149K → E in AAN71617 (PubMed:12537569).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010303146 – 161DDSQT…DISKG → EDSKKAYQEAFDIAKT in isoform C. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_000001149 – 161QTAYQ…DISKG → KNAYQEAFDIAKT in isoform VI'. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_010304185 – 193LNSPDKACQ → INSPARACH in isoform C. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77518 mRNA Translation: AAA28324.1
Y12573 Genomic DNA Translation: CAA73152.1
Y12573 Genomic DNA Translation: CAA73153.1
AE013599 Genomic DNA Translation: AAF58842.4
AE013599 Genomic DNA Translation: AAF58843.3
AE013599 Genomic DNA Translation: AAM71060.1
AE013599 Genomic DNA Translation: AAM71063.2
AE013599 Genomic DNA Translation: AAM71064.2
AE013599 Genomic DNA Translation: AAS64884.1
AE013599 Genomic DNA Translation: AAX52715.1
AE013599 Genomic DNA Translation: AAX52716.1
BT001855 mRNA Translation: AAN71617.1
BT082022 mRNA Translation: ACO72859.1
BT088441 mRNA Translation: ACR44245.1
BT099909 mRNA Translation: ACX32980.1
PIRiJC1122
RefSeqiNP_001014515.1, NM_001014515.2 [P29310-1]
NP_001014516.1, NM_001014516.2 [P29310-2]
NP_001260845.1, NM_001273916.1 [P29310-1]
NP_001260846.1, NM_001273917.1 [P29310-1]
NP_476885.2, NM_057537.4 [P29310-1]
NP_724884.1, NM_165740.3 [P29310-1]
NP_724885.1, NM_165741.3 [P29310-2]
NP_724886.1, NM_165742.2 [P29310-2]
NP_724887.2, NM_165743.3 [P29310-3]
NP_724888.2, NM_165744.3 [P29310-3]
NP_724889.2, NM_165745.3 [P29310-2]
NP_995792.1, NM_206070.2 [P29310-2]
UniGeneiDm.4705

Genome annotation databases

EnsemblMetazoaiFBtr0088412; FBpp0087500; FBgn0004907 [P29310-2]
FBtr0088413; FBpp0087501; FBgn0004907 [P29310-2]
FBtr0088414; FBpp0087502; FBgn0004907 [P29310-1]
FBtr0088415; FBpp0087503; FBgn0004907 [P29310-1]
FBtr0088416; FBpp0087504; FBgn0004907 [P29310-3]
FBtr0088417; FBpp0087505; FBgn0004907 [P29310-2]
FBtr0088418; FBpp0089337; FBgn0004907 [P29310-2]
FBtr0088419; FBpp0089338; FBgn0004907 [P29310-3]
FBtr0100182; FBpp0099539; FBgn0004907 [P29310-2]
FBtr0100183; FBpp0099540; FBgn0004907 [P29310-1]
FBtr0332916; FBpp0305136; FBgn0004907 [P29310-1]
FBtr0332917; FBpp0305137; FBgn0004907 [P29310-1]
GeneIDi36059
KEGGidme:Dmel_CG17870

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei1433Z_DROME
AccessioniPrimary (citable) accession number: P29310
Secondary accession number(s): A4UZB2
, A4UZB3, C1C593, C9QP27, O01665, Q0E9D5, Q0E9D6, Q0E9D7, Q8IGB9, Q8MKV5, Q9V5G6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: March 28, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health