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Protein

Casein kinase I homolog HRR25

Gene

HRR25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associated with repair of damaged DNA and meiosis. Phosphorylates serine and threonine. Can use casein as a substrate.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATPPROSITE-ProRule annotation
Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 239ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • protein serine/threonine kinase activity Source: SGD
  • protein tyrosine kinase activity Source: SGD

GO - Biological processi

  • attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation Source: SGD
  • DNA repair Source: SGD
  • peptidyl-tyrosine autophosphorylation Source: SGD
  • peptidyl-tyrosine phosphorylation Source: SGD
  • pexophagy Source: SGD
  • positive regulation of clathrin-mediated endocytosis Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of CVT pathway Source: SGD
  • regulation of ER to Golgi vesicle-mediated transport Source: SGD
  • regulation of protein localization Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • ribosomal small subunit biogenesis Source: SGD
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34096-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I homolog HRR25 (EC:2.7.11.1)
Gene namesi
Name:HRR25
Ordered Locus Names:YPL204W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL204W.
SGDiS000006125. HRR25.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • chromosome, centromeric region Source: SGD
  • Golgi apparatus Source: SGD
  • monopolin complex Source: SGD
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • plasma membrane Source: SGD
  • preribosome, small subunit precursor Source: GO_Central
  • spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Casein kinase I homolog HRR25PRO_0000192859Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei143 – 1431PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP29295.
PeptideAtlasiP29295.

PTM databases

iPTMnetiP29295.

Interactioni

Subunit structurei

Interacts with HRI1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-8536,EBI-8536
ATG19P351932EBI-8536,EBI-29291
ATG34Q122922EBI-8536,EBI-36362
GCN2P154425EBI-8536,EBI-330
MAM1P400653EBI-8536,EBI-22643
NNK1P360034EBI-8536,EBI-9796
PFS1P388723EBI-8536,EBI-24859

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi35980. 229 interactions.
DIPiDIP-157N.
IntActiP29295. 86 interactions.
MINTiMINT-394755.

Structurei

Secondary structure

1
494
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 148Combined sources
Beta strandi23 – 275Combined sources
Turni29 – 313Combined sources
Beta strandi34 – 396Combined sources
Turni42 – 454Combined sources
Helixi49 – 5911Combined sources
Beta strandi68 – 747Combined sources
Beta strandi77 – 837Combined sources
Helixi89 – 957Combined sources
Helixi102 – 12120Combined sources
Helixi131 – 1333Combined sources
Helixi140 – 1423Combined sources
Turni159 – 1613Combined sources
Helixi177 – 1793Combined sources
Helixi182 – 1854Combined sources
Helixi192 – 20716Combined sources
Helixi221 – 23414Combined sources
Helixi237 – 2404Combined sources
Turni241 – 2433Combined sources
Helixi246 – 25611Combined sources
Helixi266 – 27914Combined sources
Helixi289 – 29810Combined sources
Helixi334 – 34310Combined sources
Turni345 – 3473Combined sources
Helixi361 – 3644Combined sources
Helixi366 – 3705Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4XHLX-ray3.01A1-394[»]
ProteinModelPortaliP29295.
SMRiP29295. Positions 4-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 278270Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi395 – 494100Gln/Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182055.
InParanoidiP29295.
KOiK14758.
OMAiNTHRHIP.
OrthoDBiEOG7WQ832.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLRVGRKFR IGRKIGSGSF GDIYHGTNLI SGEEVAIKLE SIRSRHPQLD
60 70 80 90 100
YESRVYRYLS GGVGIPFIRW FGREGEYNAM VIDLLGPSLE DLFNYCHRRF
110 120 130 140 150
SFKTVIMLAL QMFCRIQYIH GRSFIHRDIK PDNFLMGVGR RGSTVHVIDF
160 170 180 190 200
GLSKKYRDFN THRHIPYREN KSLTGTARYA SVNTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLIYFCKGS LPWQGLKATT KKQKYDRIME KKLNVSVETL CSGLPLEFQE
260 270 280 290 300
YMAYCKNLKF DEKPDYLFLA RLFKDLSIKL EYHNDHLFDW TMLRYTKAMV
310 320 330 340 350
EKQRDLLIEK GDLNANSNAA SASNSTDNKS ETFNKIKLLA MKKFPTHFHY
360 370 380 390 400
YKNEDKHNPS PEEIKQQTIL NNNAASSLPE ELLNALDKGM ENLRQQQPQQ
410 420 430 440 450
QVQSSQPQPQ PQQLQQQPNG QRPNYYPEPL LQQQQRDSQE QQQQVPMATT
460 470 480 490
RATQYPPQIN SNNFNTNQAS VPPQMRSNPQ QPPQDKPAGQ SIWL
Length:494
Mass (Da):57,340
Last modified:December 1, 1992 - v1
Checksum:iC4D3107A470E8BAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68605 Genomic DNA. Translation: AAA34687.1.
Z73560 Genomic DNA. Translation: CAA97918.1.
BK006949 Genomic DNA. Translation: DAA11231.1.
PIRiA40860.
RefSeqiNP_015120.1. NM_001184018.1.

Genome annotation databases

EnsemblFungiiYPL204W; YPL204W; YPL204W.
GeneIDi855897.
KEGGisce:YPL204W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68605 Genomic DNA. Translation: AAA34687.1.
Z73560 Genomic DNA. Translation: CAA97918.1.
BK006949 Genomic DNA. Translation: DAA11231.1.
PIRiA40860.
RefSeqiNP_015120.1. NM_001184018.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4XHLX-ray3.01A1-394[»]
ProteinModelPortaliP29295.
SMRiP29295. Positions 4-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35980. 229 interactions.
DIPiDIP-157N.
IntActiP29295. 86 interactions.
MINTiMINT-394755.

PTM databases

iPTMnetiP29295.

Proteomic databases

MaxQBiP29295.
PeptideAtlasiP29295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL204W; YPL204W; YPL204W.
GeneIDi855897.
KEGGisce:YPL204W.

Organism-specific databases

EuPathDBiFungiDB:YPL204W.
SGDiS000006125. HRR25.

Phylogenomic databases

GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182055.
InParanoidiP29295.
KOiK14758.
OMAiNTHRHIP.
OrthoDBiEOG7WQ832.

Enzyme and pathway databases

BioCyciYEAST:G3O-34096-MONOMER.
BRENDAi2.7.11.1. 984.

Miscellaneous databases

NextBioi980580.
PROiP29295.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA."
    Hoekstra M.F., Liskay R.M., Ou A.C., Demaggio A.J., Burbee D.G., Heffron F.
    Science 253:1031-1034(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The budding yeast HRR25 gene product is a casein kinase I isoform."
    Demaggio A.J., Lindberg R.A., Hunter T., Hoekstra M.F.
    Proc. Natl. Acad. Sci. U.S.A. 89:7008-7012(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes."
    Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E.
    EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes."
    Fasolo J., Sboner A., Sun M.G., Yu H., Chen R., Sharon D., Kim P.M., Gerstein M., Snyder M.
    Genes Dev. 25:767-778(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HRI1.

Entry informationi

Entry nameiHRR25_YEAST
AccessioniPrimary (citable) accession number: P29295
Secondary accession number(s): D6W3G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 11, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.