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Protein

Casein kinase I homolog HRR25

Gene

HRR25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associated with repair of damaged DNA and meiosis. Phosphorylates serine and threonine. Can use casein as a substrate.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD
  • protein tyrosine kinase activity Source: SGD

GO - Biological processi

  • attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation Source: SGD
  • DNA repair Source: SGD
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-tyrosine autophosphorylation Source: SGD
  • peptidyl-tyrosine phosphorylation Source: SGD
  • pexophagy Source: SGD
  • positive regulation of clathrin-dependent endocytosis Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of cell shape Source: GO_Central
  • regulation of CVT pathway Source: SGD
  • regulation of ER to Golgi vesicle-mediated transport Source: SGD
  • regulation of protein localization Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • ribosomal small subunit biogenesis Source: SGD
  • tRNA wobble uridine modification Source: SGD
  • Wnt signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34096-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I homolog HRR25 (EC:2.7.11.1)
Gene namesi
Name:HRR25
Ordered Locus Names:YPL204W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL204W.
SGDiS000006125. HRR25.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • chromosome, centromeric region Source: SGD
  • Golgi apparatus Source: SGD
  • monopolin complex Source: SGD
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • plasma membrane Source: SGD
  • preribosome, small subunit precursor Source: GO_Central
  • spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928591 – 494Casein kinase I homolog HRR25Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei143PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP29295.
PRIDEiP29295.

PTM databases

iPTMnetiP29295.

Interactioni

Subunit structurei

Interacts with HRI1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-8536,EBI-8536
ATG19P351932EBI-8536,EBI-29291
ATG34Q122922EBI-8536,EBI-36362
GCN2P154425EBI-8536,EBI-330
MAM1P400653EBI-8536,EBI-22643
NNK1P360034EBI-8536,EBI-9796
PFS1P388723EBI-8536,EBI-24859

Protein-protein interaction databases

BioGridi35980. 230 interactors.
DIPiDIP-157N.
IntActiP29295. 86 interactors.
MINTiMINT-394755.

Structurei

Secondary structure

1494
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Beta strandi9 – 17Combined sources9
Beta strandi22 – 28Combined sources7
Turni29 – 31Combined sources3
Beta strandi34 – 41Combined sources8
Turni42 – 45Combined sources4
Helixi49 – 58Combined sources10
Turni59 – 61Combined sources3
Beta strandi68 – 74Combined sources7
Beta strandi77 – 83Combined sources7
Beta strandi85 – 88Combined sources4
Helixi89 – 95Combined sources7
Turni96 – 98Combined sources3
Helixi102 – 121Combined sources20
Helixi131 – 133Combined sources3
Beta strandi134 – 138Combined sources5
Helixi139 – 141Combined sources3
Beta strandi145 – 147Combined sources3
Beta strandi154 – 157Combined sources4
Turni159 – 161Combined sources3
Helixi177 – 179Combined sources3
Helixi182 – 185Combined sources4
Helixi192 – 208Combined sources17
Turni212 – 215Combined sources4
Helixi221 – 234Combined sources14
Helixi237 – 240Combined sources4
Turni241 – 243Combined sources3
Helixi246 – 257Combined sources12
Helixi266 – 279Combined sources14
Helixi289 – 307Combined sources19
Helixi330 – 343Combined sources14
Turni345 – 347Combined sources3
Helixi361 – 364Combined sources4
Helixi366 – 373Combined sources8
Helixi379 – 386Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XHLX-ray3.01A1-394[»]
5CYZX-ray1.84A1-394[»]
5CZOX-ray2.89A/B1-394[»]
ProteinModelPortaliP29295.
SMRiP29295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 278Protein kinasePROSITE-ProRule annotationAdd BLAST270

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi395 – 494Gln/Pro-richAdd BLAST100

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182055.
InParanoidiP29295.
KOiK14758.
OMAiEYMAYCK.
OrthoDBiEOG092C30PR.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLRVGRKFR IGRKIGSGSF GDIYHGTNLI SGEEVAIKLE SIRSRHPQLD
60 70 80 90 100
YESRVYRYLS GGVGIPFIRW FGREGEYNAM VIDLLGPSLE DLFNYCHRRF
110 120 130 140 150
SFKTVIMLAL QMFCRIQYIH GRSFIHRDIK PDNFLMGVGR RGSTVHVIDF
160 170 180 190 200
GLSKKYRDFN THRHIPYREN KSLTGTARYA SVNTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLIYFCKGS LPWQGLKATT KKQKYDRIME KKLNVSVETL CSGLPLEFQE
260 270 280 290 300
YMAYCKNLKF DEKPDYLFLA RLFKDLSIKL EYHNDHLFDW TMLRYTKAMV
310 320 330 340 350
EKQRDLLIEK GDLNANSNAA SASNSTDNKS ETFNKIKLLA MKKFPTHFHY
360 370 380 390 400
YKNEDKHNPS PEEIKQQTIL NNNAASSLPE ELLNALDKGM ENLRQQQPQQ
410 420 430 440 450
QVQSSQPQPQ PQQLQQQPNG QRPNYYPEPL LQQQQRDSQE QQQQVPMATT
460 470 480 490
RATQYPPQIN SNNFNTNQAS VPPQMRSNPQ QPPQDKPAGQ SIWL
Length:494
Mass (Da):57,340
Last modified:December 1, 1992 - v1
Checksum:iC4D3107A470E8BAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68605 Genomic DNA. Translation: AAA34687.1.
Z73560 Genomic DNA. Translation: CAA97918.1.
BK006949 Genomic DNA. Translation: DAA11231.1.
PIRiA40860.
RefSeqiNP_015120.1. NM_001184018.1.

Genome annotation databases

EnsemblFungiiYPL204W; YPL204W; YPL204W.
GeneIDi855897.
KEGGisce:YPL204W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68605 Genomic DNA. Translation: AAA34687.1.
Z73560 Genomic DNA. Translation: CAA97918.1.
BK006949 Genomic DNA. Translation: DAA11231.1.
PIRiA40860.
RefSeqiNP_015120.1. NM_001184018.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XHLX-ray3.01A1-394[»]
5CYZX-ray1.84A1-394[»]
5CZOX-ray2.89A/B1-394[»]
ProteinModelPortaliP29295.
SMRiP29295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35980. 230 interactors.
DIPiDIP-157N.
IntActiP29295. 86 interactors.
MINTiMINT-394755.

PTM databases

iPTMnetiP29295.

Proteomic databases

MaxQBiP29295.
PRIDEiP29295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL204W; YPL204W; YPL204W.
GeneIDi855897.
KEGGisce:YPL204W.

Organism-specific databases

EuPathDBiFungiDB:YPL204W.
SGDiS000006125. HRR25.

Phylogenomic databases

GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182055.
InParanoidiP29295.
KOiK14758.
OMAiEYMAYCK.
OrthoDBiEOG092C30PR.

Enzyme and pathway databases

BioCyciYEAST:G3O-34096-MONOMER.
BRENDAi2.7.11.1. 984.

Miscellaneous databases

PROiP29295.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHRR25_YEAST
AccessioniPrimary (citable) accession number: P29295
Secondary accession number(s): D6W3G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.