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Protein

Acrosin

Gene

Acr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Charge relay systemBy similarity
Active sitei143 – 1431Charge relay systemBy similarity
Active sitei241 – 2411Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

  • acrosome reaction Source: UniProtKB
  • activation of adenylate cyclase activity Source: UniProtKB
  • binding of sperm to zona pellucida Source: Ensembl
  • penetration of zona pellucida Source: RGD
  • response to steroid hormone Source: RGD
  • single fertilization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.10. 5301.
ReactomeiR-RNO-1300645. Acrosome Reaction.

Protein family/group databases

MEROPSiS01.223.

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Cleaved into the following 2 chains:
Gene namesi
Name:Acr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2024. Acr.

Subcellular locationi

GO - Cellular componenti

  • acrosomal matrix Source: HGNC
  • acrosomal vesicle Source: RGD
  • Golgi-associated vesicle Source: RGD
  • nucleus Source: Ensembl
  • protein complex Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 437418AcrosinPRO_0000027534Add
BLAST
Chaini20 – 4223Acrosin light chainPRO_0000027535Add
BLAST
Chaini43 – 345303Acrosin heavy chainPRO_0000027536Add
BLAST
Propeptidei346 – 43792Pro-richBy similarityPRO_0000027537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi22 – 221N-linked (GlcNAc...)By similarity
Disulfide bondi25 ↔ 155Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi29 ↔ 163Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi74 ↔ 90PROSITE-ProRule annotation
Disulfide bondi178 ↔ 247PROSITE-ProRule annotation
Disulfide bondi210 ↔ 226PROSITE-ProRule annotation
Glycosylationi211 – 2111N-linked (GlcNAc...)By similarity
Disulfide bondi237 ↔ 267PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP29293.
PRIDEiP29293.

Expressioni

Gene expression databases

BgeeiENSRNOG00000029762.
ExpressionAtlasiP29293. baseline.
GenevisibleiP29293. RN.

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046900.

Structurei

3D structure databases

ProteinModelPortaliP29293.
SMRiP29293. Positions 43-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 291249Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00840000129691.
HOGENOMiHOG000033792.
HOVERGENiHBG013304.
InParanoidiP29293.
KOiK01317.
OMAiKKVYDWR.
OrthoDBiEOG091G0AH5.
PhylomeDBiP29293.
TreeFamiTF335943.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEMLPTVVA LVLAVSVVAK DNTTCDGPCG LRFRQNPQAG IRIVGGQTSS
60 70 80 90 100
PGAWPWMVSL QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDW
110 120 130 140 150
RLVFGAHEIE YGRNKPVKEP QQERYVQKIV IHEKYNAVTE GNDIALLKVT
160 170 180 190 200
PPVTCGDFVG PGCLPHFKSG PPRIPHTCYV TGWGYIKDNA PRPSPVLMEA
210 220 230 240 250
RVDLIDLDLC NSTQWYNGRV TSTNVCAGYP EGKIDTCQGD SGGPLMCRDS
260 270 280 290 300
VDSPFVIVGI TSWGVGCARA KRPGVYTATW DYLDWIASKI GPTALHLIQP
310 320 330 340 350
ATPHPPTTQQ PVISFHPPSI HPPWYFQHLS PRPPHLRPPR PLLHQPSSVH
360 370 380 390 400
TSSAPVIPLL SLLTPVQPVS FTLAAYHTRH HTTLSFARRL QHLIEALKMR
410 420 430
TYPIKYPSRY SGPVNYQHRF STFEPLSNKP SEPLLHS
Length:437
Mass (Da):48,623
Last modified:May 16, 2006 - v2
Checksum:i882CF0F7896A927E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 265Missing in CAA41441 (PubMed:1802037).Curated
Sequence conflicti51 – 522PG → RW (PubMed:1932123).Curated
Sequence conflicti51 – 522PG → RW (PubMed:1802037).Curated
Sequence conflicti95 – 951K → KR in CAA41441 (PubMed:1802037).Curated
Sequence conflicti250 – 2534SVDS → TRRQ (PubMed:1932123).Curated
Sequence conflicti250 – 2534SVDS → TRRQ (PubMed:1802037).Curated
Sequence conflicti255 – 26814FVIVG…GVGCA → LCDRGDHELGGRLC (PubMed:1932123).CuratedAdd
BLAST
Sequence conflicti255 – 26814FVIVG…GVGCA → LCDRGDHELGGRLC (PubMed:1802037).CuratedAdd
BLAST
Sequence conflicti320 – 33718IHPPW…PPHLR → TPPSLVLPTPVSSAALPT (PubMed:1932123).CuratedAdd
BLAST
Sequence conflicti320 – 33718IHPPW…PPHLR → TPPSLVLPTPVSSAALPT (PubMed:1802037).CuratedAdd
BLAST
Sequence conflicti388 – 3892RR → SA (PubMed:1932123).Curated
Sequence conflicti388 – 3892RR → SA (PubMed:1802037).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59254 mRNA. Translation: CAA41947.1.
X58550 Genomic DNA. Translation: CAA41441.2.
BC097345 mRNA. Translation: AAH97345.1.
PIRiS18407.
RefSeqiNP_036622.2. NM_012490.2.
XP_008763887.1. XM_008765665.1.
XP_008763888.1. XM_008765666.1.
UniGeneiRn.10423.

Genome annotation databases

EnsembliENSRNOT00000045647; ENSRNOP00000046900; ENSRNOG00000029762.
GeneIDi24163.
KEGGirno:24163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59254 mRNA. Translation: CAA41947.1.
X58550 Genomic DNA. Translation: CAA41441.2.
BC097345 mRNA. Translation: AAH97345.1.
PIRiS18407.
RefSeqiNP_036622.2. NM_012490.2.
XP_008763887.1. XM_008765665.1.
XP_008763888.1. XM_008765666.1.
UniGeneiRn.10423.

3D structure databases

ProteinModelPortaliP29293.
SMRiP29293. Positions 43-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046900.

Protein family/group databases

MEROPSiS01.223.

Proteomic databases

PaxDbiP29293.
PRIDEiP29293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000045647; ENSRNOP00000046900; ENSRNOG00000029762.
GeneIDi24163.
KEGGirno:24163.

Organism-specific databases

CTDi49.
RGDi2024. Acr.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00840000129691.
HOGENOMiHOG000033792.
HOVERGENiHBG013304.
InParanoidiP29293.
KOiK01317.
OMAiKKVYDWR.
OrthoDBiEOG091G0AH5.
PhylomeDBiP29293.
TreeFamiTF335943.

Enzyme and pathway databases

BRENDAi3.4.21.10. 5301.
ReactomeiR-RNO-1300645. Acrosome Reaction.

Miscellaneous databases

PROiP29293.

Gene expression databases

BgeeiENSRNOG00000029762.
ExpressionAtlasiP29293. baseline.
GenevisibleiP29293. RN.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACRO_RAT
AccessioniPrimary (citable) accession number: P29293
Secondary accession number(s): Q4QR89, Q9QWF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: May 16, 2006
Last modified: September 7, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.