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Protein

Nitrogen fixation regulation protein FixK

Gene

fixK

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

B.japonicum has two FixLJ-dependent FixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site.

Miscellaneous

Possesses 4 cysteines which may bind a metal ion (possibly iron).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi189 – 208H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processNitrogen fixation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen fixation regulation protein FixK
Gene namesi
Name:fixK
Ordered Locus Names:bll6061
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001571 – 237Nitrogen fixation regulation protein FixKAdd BLAST237

Expressioni

Inductioni

Its expression is maximal under reduced oxygen conditions and depends on the two-component regulatory system FixLJ.

Interactioni

Protein-protein interaction databases

STRINGi224911.bll6061.

Structurei

3D structure databases

ProteinModelPortaliP29286.
SMRiP29286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 229HTH crp-typePROSITE-ProRule annotationAdd BLAST74

Phylogenomic databases

eggNOGiENOG4105DS0. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000186462.
InParanoidiP29286.
KOiK01420.
OMAiPARRIME.
OrthoDBiPOG091H0447.
PhylomeDBiP29286.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiView protein in InterPro
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
PRINTSiPR00034. HTHCRP.
SMARTiView protein in SMART
SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiView protein in PROSITE
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P29286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSVVMIEP NGHFCSDCAI RTSAVCSSLD AAELREFEHL GRRVHFSSGE
60 70 80 90 100
TVFSEEDITT SFYNVLEGVM RLYKLLPDGR RQIVGFALPG DFLGMNLSGR
110 120 130 140 150
HNFSADAIGA VTVCQFAKAP FGRFIEERPQ LLRRINELAI RELSQARDHM
160 170 180 190 200
VLLGRRSADE KVAAFLLGWR ERLLALKGAS DTVPLPMSRQ DIADYLGLTI
210 220 230
ETVSRTFTKL ERHGAIAIIH GGISLLDPAR VEALAAA
Length:237
Mass (Da):26,168
Last modified:December 1, 1992 - v1
Checksum:i90955FEA2CA6D523
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86805 Genomic DNA. Translation: AAA26209.1.
BA000040 Genomic DNA. Translation: BAC51326.1.
PIRiB42371.
RefSeqiNP_772701.1. NC_004463.1.
WP_011088802.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaiBAC51326; BAC51326; BAC51326.
GeneIDi1051938.
KEGGibja:bll6061.
PATRICi21195900. VBIBraJap65052_6185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86805 Genomic DNA. Translation: AAA26209.1.
BA000040 Genomic DNA. Translation: BAC51326.1.
PIRiB42371.
RefSeqiNP_772701.1. NC_004463.1.
WP_011088802.1. NZ_CP011360.1.

3D structure databases

ProteinModelPortaliP29286.
SMRiP29286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.bll6061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC51326; BAC51326; BAC51326.
GeneIDi1051938.
KEGGibja:bll6061.
PATRICi21195900. VBIBraJap65052_6185.

Phylogenomic databases

eggNOGiENOG4105DS0. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000186462.
InParanoidiP29286.
KOiK01420.
OMAiPARRIME.
OrthoDBiPOG091H0447.
PhylomeDBiP29286.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiView protein in InterPro
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
PRINTSiPR00034. HTHCRP.
SMARTiView protein in SMART
SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiView protein in PROSITE
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFIXK_BRADU
AccessioniPrimary (citable) accession number: P29286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: March 15, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.