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P29286

- FIXK_BRADU

UniProt

P29286 - FIXK_BRADU

Protein

Nitrogen fixation regulation protein FixK

Gene

fixK

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    B.japonicum has two FixLJ-dependent FixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi189 – 20820H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. nitrogen fixation Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Nitrogen fixation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-6108-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrogen fixation regulation protein FixK
    Gene namesi
    Name:fixK
    Ordered Locus Names:bll6061
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237Nitrogen fixation regulation protein FixKPRO_0000100157Add
    BLAST

    Expressioni

    Inductioni

    Its expression is maximal under reduced oxygen conditions and depends on the two-component regulatory system FixLJ.

    Interactioni

    Protein-protein interaction databases

    STRINGi224911.bll6061.

    Structurei

    3D structure databases

    ProteinModelPortaliP29286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini156 – 22974HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000186462.
    KOiK01420.
    OrthoDBiEOG6TBHH3.
    PhylomeDBiP29286.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29286-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPSVVMIEP NGHFCSDCAI RTSAVCSSLD AAELREFEHL GRRVHFSSGE    50
    TVFSEEDITT SFYNVLEGVM RLYKLLPDGR RQIVGFALPG DFLGMNLSGR 100
    HNFSADAIGA VTVCQFAKAP FGRFIEERPQ LLRRINELAI RELSQARDHM 150
    VLLGRRSADE KVAAFLLGWR ERLLALKGAS DTVPLPMSRQ DIADYLGLTI 200
    ETVSRTFTKL ERHGAIAIIH GGISLLDPAR VEALAAA 237
    Length:237
    Mass (Da):26,168
    Last modified:December 1, 1992 - v1
    Checksum:i90955FEA2CA6D523
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86805 Genomic DNA. Translation: AAA26209.1.
    BA000040 Genomic DNA. Translation: BAC51326.1.
    PIRiB42371.
    RefSeqiNP_772701.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC51326; BAC51326; BAC51326.
    GeneIDi1051938.
    KEGGibja:bll6061.
    PATRICi21195900. VBIBraJap65052_6185.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86805 Genomic DNA. Translation: AAA26209.1 .
    BA000040 Genomic DNA. Translation: BAC51326.1 .
    PIRi B42371.
    RefSeqi NP_772701.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali P29286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.bll6061.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC51326 ; BAC51326 ; BAC51326 .
    GeneIDi 1051938.
    KEGGi bja:bll6061.
    PATRICi 21195900. VBIBraJap65052_6185.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000186462.
    KOi K01420.
    OrthoDBi EOG6TBHH3.
    PhylomeDBi P29286.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-6108-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a fixLJ-regulated Bradyrhizobium japonicum gene sharing similarity with the Escherichia coli fnr and Rhizobium meliloti fixK genes."
      Anthamatten D., Scherb B., Hennecke H.
      J. Bacteriol. 174:2111-2120(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: USDA 110spc4.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiFIXK_BRADU
    AccessioniPrimary (citable) accession number: P29286
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Possesses 4 cysteines which may bind a metal ion (possibly iron).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3