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P29286 (FIXK_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrogen fixation regulation protein FixK
Gene names
Name:fixK
Ordered Locus Names:bll6061
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

B.japonicum has two FixLJ-dependent FixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site.

Induction

Its expression is maximal under reduced oxygen conditions and depends on the two-component regulatory system FixLJ.

Miscellaneous

Possesses 4 cysteines which may bind a metal ion (possibly iron).

Sequence similarities

Contains 1 cyclic nucleotide-binding domain.

Contains 1 HTH crp-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237Nitrogen fixation regulation protein FixK
PRO_0000100157

Regions

Domain156 – 22974HTH crp-type
DNA binding189 – 20820H-T-H motif By similarity

Sequences

Sequence LengthMass (Da)Tools
P29286 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 90955FEA2CA6D523

FASTA23726,168
        10         20         30         40         50         60 
MKPSVVMIEP NGHFCSDCAI RTSAVCSSLD AAELREFEHL GRRVHFSSGE TVFSEEDITT 

        70         80         90        100        110        120 
SFYNVLEGVM RLYKLLPDGR RQIVGFALPG DFLGMNLSGR HNFSADAIGA VTVCQFAKAP 

       130        140        150        160        170        180 
FGRFIEERPQ LLRRINELAI RELSQARDHM VLLGRRSADE KVAAFLLGWR ERLLALKGAS 

       190        200        210        220        230 
DTVPLPMSRQ DIADYLGLTI ETVSRTFTKL ERHGAIAIIH GGISLLDPAR VEALAAA 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a fixLJ-regulated Bradyrhizobium japonicum gene sharing similarity with the Escherichia coli fnr and Rhizobium meliloti fixK genes."
Anthamatten D., Scherb B., Hennecke H.
J. Bacteriol. 174:2111-2120(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: USDA 110spc4.
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86805 Genomic DNA. Translation: AAA26209.1.
BA000040 Genomic DNA. Translation: BAC51326.1.
PIRB42371.
RefSeqNP_772701.1. NC_004463.1.

3D structure databases

ProteinModelPortalP29286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll6061.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC51326; BAC51326; BAC51326.
GeneID1051938.
KEGGbja:bll6061.
PATRIC21195900. VBIBraJap65052_6185.

Phylogenomic databases

eggNOGCOG0664.
HOGENOMHOG000186462.
KOK01420.
OrthoDBEOG6TBHH3.
PhylomeDBP29286.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-6108-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSPR00034. HTHCRP.
SMARTSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFIXK_BRADU
AccessionPrimary (citable) accession number: P29286
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families