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P29281

- CRP_HAEIN

UniProt

P29281 - CRP_HAEIN

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Protein

cAMP-activated global transcriptional regulator CRP

Gene
crp, cap, HI_0957
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

A global transcription regulator required for bacterial competence. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to regulate transcription at 54 promoters (1 Publication). There are 2 consensus subclasses; CRP-N (above) and CRP-S (T6 changed to C and A17 changed to G) which obligatorily also requires Sxy for expression. CRP-S sites bind DNA but do not activate transcription in the absence of Sxy. Probably induces a severe bend in DNA. Probably acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei111 – 1111Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Sitei116 – 1161Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 777cAMP 1 By similarity
Nucleotide bindingi86 – 883cAMP 1 By similarity
Nucleotide bindingi97 – 982cAMP 1 By similarity
Nucleotide bindingi142 – 1432cAMP 1 By similarity
Nucleotide bindingi150 – 1512cAMP 2 By similarity
Nucleotide bindingi185 – 19511cAMP 2 By similarityAdd
BLAST
DNA bindingi194 – 2007H-T-H motif By similarity

GO - Molecular functioni

  1. cAMP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. establishment of competence for transformation Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Competence, Transcription, Transcription regulation

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-activated global transcriptional regulator CRP
Alternative name(s):
Catabolite activator protein
Short name:
CAP
cAMP receptor protein
Short name:
CRP
cAMP regulatory protein
Gene namesi
Name:crp
Synonyms:cap
Ordered Locus Names:HI_0957
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Loss of competence, the ability to bind, take-up and integrate exogenous DNA. Loss of the ability to ferment ribose or xylose.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224cAMP-activated global transcriptional regulator CRPPRO_0000100147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei115 – 1151N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Expressioni

Inductioni

Constitutively expressed; slightly induced on shifting to starvation media.2 Publications

Interactioni

Subunit structurei

Homodimer, which upon binding cAMP is able to bind DNA Inferred. Binds RNA polymerase subunit RpoA By similarity.

Protein-protein interaction databases

STRINGi71421.HI0957.

Structurei

3D structure databases

ProteinModelPortaliP29281.
SMRiP29281. Positions 21-222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 22473HTH crp-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 363Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Regioni67 – 737Activating region 3 (AR3); probably contacts sigma-70 (RpoD) By similarity
Regioni168 – 17710Activating region 1 (AR1); probably contacts the C-terminus of RpoA By similarity

Domaini

The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0664.
KOiK10914.
OMAiINCTHED.
OrthoDBiEOG69GZGV.
PhylomeDBiP29281.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29281-1 [UniParc]FASTAAdd to Basket

« Hide

MSNELTEIDE VVTSSQEEAT QRDPVLDWFL THCHLHKYPA KSTLIHAGED    50
ATTLYYVIKG SVMVSSKDDE GKEMILTYLG AGQFFGEAGL FDEGSKRSAW 100
VKTKTTCEIA EISYKKYRQL IQANPEILMF LTAQLARRLQ NTSRQVTNLA 150
FLDVAGRIAQ TLMNLAKQPE AMTHPDGMQI KITRQEIGQM VGCSRETVGR 200
IIKMLEDQNL IHAHGKTIVV YGAR 224
Length:224
Mass (Da):25,152
Last modified:December 1, 1992 - v1
Checksum:i421B7790A48FF50C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77207 Genomic DNA. Translation: AAA24952.1.
L42023 Genomic DNA. Translation: AAC22618.1.
PIRiB38225.
RefSeqiNP_439118.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22618; AAC22618; HI_0957.
GeneIDi949963.
KEGGihin:HI0957.
PATRICi20190573. VBIHaeInf48452_0999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77207 Genomic DNA. Translation: AAA24952.1 .
L42023 Genomic DNA. Translation: AAC22618.1 .
PIRi B38225.
RefSeqi NP_439118.1. NC_000907.1.

3D structure databases

ProteinModelPortali P29281.
SMRi P29281. Positions 21-222.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22618 ; AAC22618 ; HI_0957 .
GeneIDi 949963.
KEGGi hin:HI0957.
PATRICi 20190573. VBIHaeInf48452_0999.

Phylogenomic databases

eggNOGi COG0664.
KOi K10914.
OMAi INCTHED.
OrthoDBi EOG69GZGV.
PhylomeDBi P29281.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view ]
PRINTSi PR00034. HTHCRP.
SMARTi SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding cAMP receptor protein is required for competence development in Haemophilus influenzae Rd."
    Chandler M.S.
    Proc. Natl. Acad. Sci. U.S.A. 89:1626-1630(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PROBABLE AUTOREGULATION, DISRUPTION PHENOTYPE.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  3. "The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence."
    Dorocicz I.R., Williams P.M., Redfield R.J.
    J. Bacteriol. 175:7142-7149(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  4. "A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae."
    Redfield R.J., Cameron A.D., Qian Q., Hinds J., Ali T.R., Kroll J.S., Langford P.R.
    J. Mol. Biol. 347:735-747(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULON, INDUCTION.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  5. "CRP binding and transcription activation at CRP-S sites."
    Cameron A.D., Redfield R.J.
    J. Mol. Biol. 383:313-323(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiCRP_HAEIN
AccessioniPrimary (citable) accession number: P29281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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