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P29281

- CRP_HAEIN

UniProt

P29281 - CRP_HAEIN

Protein

cAMP-activated global transcriptional regulator CRP

Gene

crp

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    A global transcription regulator required for bacterial competence. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to regulate transcription at 54 promoters (PubMed:15769466). There are 2 consensus subclasses; CRP-N (above) and CRP-S (T6 changed to C and A17 changed to G) which obligatorily also requires Sxy for expression. CRP-S sites bind DNA but do not activate transcription in the absence of Sxy. Probably induces a severe bend in DNA. Probably acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei111 – 1111Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
    Sitei116 – 1161Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi71 – 777cAMP 1By similarity
    Nucleotide bindingi86 – 883cAMP 1By similarity
    Nucleotide bindingi97 – 982cAMP 1By similarity
    Nucleotide bindingi142 – 1432cAMP 1By similarity
    Nucleotide bindingi150 – 1512cAMP 2By similarity
    Nucleotide bindingi185 – 19511cAMP 2By similarityAdd
    BLAST
    DNA bindingi194 – 2007H-T-H motifPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cAMP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. establishment of competence for transformation Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Competence, Transcription, Transcription regulation

    Keywords - Ligandi

    cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-activated global transcriptional regulator CRP
    Alternative name(s):
    Catabolite activator protein
    Short name:
    CAP
    cAMP receptor protein
    Short name:
    CRP
    cAMP regulatory protein
    Gene namesi
    Name:crp
    Synonyms:cap
    Ordered Locus Names:HI_0957
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    Pathology & Biotechi

    Disruption phenotypei

    Loss of competence, the ability to bind, take-up and integrate exogenous DNA. Loss of the ability to ferment ribose or xylose.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224cAMP-activated global transcriptional regulator CRPPRO_0000100147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei115 – 1151N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Expressioni

    Inductioni

    Constitutively expressed; slightly induced on shifting to starvation media.1 Publication

    Interactioni

    Subunit structurei

    Homodimer, which upon binding cAMP is able to bind DNA Probable. Binds RNA polymerase subunit RpoA By similarity.By similarityCurated

    Protein-protein interaction databases

    STRINGi71421.HI0957.

    Structurei

    3D structure databases

    ProteinModelPortaliP29281.
    SMRiP29281. Positions 21-222.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini152 – 22473HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni34 – 363Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
    Regioni67 – 737Activating region 3 (AR3); probably contacts sigma-70 (RpoD)By similarity
    Regioni168 – 17710Activating region 1 (AR1); probably contacts the C-terminus of RpoABy similarity

    Domaini

    The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    KOiK10914.
    OMAiINCTHED.
    OrthoDBiEOG69GZGV.
    PhylomeDBiP29281.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29281-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNELTEIDE VVTSSQEEAT QRDPVLDWFL THCHLHKYPA KSTLIHAGED    50
    ATTLYYVIKG SVMVSSKDDE GKEMILTYLG AGQFFGEAGL FDEGSKRSAW 100
    VKTKTTCEIA EISYKKYRQL IQANPEILMF LTAQLARRLQ NTSRQVTNLA 150
    FLDVAGRIAQ TLMNLAKQPE AMTHPDGMQI KITRQEIGQM VGCSRETVGR 200
    IIKMLEDQNL IHAHGKTIVV YGAR 224
    Length:224
    Mass (Da):25,152
    Last modified:December 1, 1992 - v1
    Checksum:i421B7790A48FF50C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77207 Genomic DNA. Translation: AAA24952.1.
    L42023 Genomic DNA. Translation: AAC22618.1.
    PIRiB38225.
    RefSeqiNP_439118.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22618; AAC22618; HI_0957.
    GeneIDi949963.
    KEGGihin:HI0957.
    PATRICi20190573. VBIHaeInf48452_0999.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77207 Genomic DNA. Translation: AAA24952.1 .
    L42023 Genomic DNA. Translation: AAC22618.1 .
    PIRi B38225.
    RefSeqi NP_439118.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P29281.
    SMRi P29281. Positions 21-222.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0957.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22618 ; AAC22618 ; HI_0957 .
    GeneIDi 949963.
    KEGGi hin:HI0957.
    PATRICi 20190573. VBIHaeInf48452_0999.

    Phylogenomic databases

    eggNOGi COG0664.
    KOi K10914.
    OMAi INCTHED.
    OrthoDBi EOG69GZGV.
    PhylomeDBi P29281.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene encoding cAMP receptor protein is required for competence development in Haemophilus influenzae Rd."
      Chandler M.S.
      Proc. Natl. Acad. Sci. U.S.A. 89:1626-1630(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PROBABLE AUTOREGULATION, DISRUPTION PHENOTYPE.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    3. "The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence."
      Dorocicz I.R., Williams P.M., Redfield R.J.
      J. Bacteriol. 175:7142-7149(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    4. "A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae."
      Redfield R.J., Cameron A.D., Qian Q., Hinds J., Ali T.R., Kroll J.S., Langford P.R.
      J. Mol. Biol. 347:735-747(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, REGULON, INDUCTION.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    5. "CRP binding and transcription activation at CRP-S sites."
      Cameron A.D., Redfield R.J.
      J. Mol. Biol. 383:313-323(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiCRP_HAEIN
    AccessioniPrimary (citable) accession number: P29281
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3