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Protein

cAMP-activated global transcriptional regulator CRP

Gene

crp

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A global transcription regulator required for bacterial competence. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to regulate transcription at 54 promoters (PubMed:15769466). There are 2 consensus subclasses; CRP-N (above) and CRP-S (T6 changed to C and A17 changed to G) which obligatorily also requires Sxy for expression. CRP-S sites bind DNA but do not activate transcription in the absence of Sxy. Probably induces a severe bend in DNA. Probably acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity1
Sitei116Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 77cAMP 1By similarity7
Nucleotide bindingi86 – 88cAMP 1By similarity3
Nucleotide bindingi97 – 98cAMP 1By similarity2
Nucleotide bindingi142 – 143cAMP 1By similarity2
Nucleotide bindingi150 – 151cAMP 2By similarity2
Nucleotide bindingi185 – 195cAMP 2By similarityAdd BLAST11
DNA bindingi194 – 200H-T-H motifPROSITE-ProRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Competence, Transcription, Transcription regulation

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-activated global transcriptional regulator CRP
Alternative name(s):
Catabolite activator protein
Short name:
CAP
cAMP receptor protein
Short name:
CRP
cAMP regulatory protein
Gene namesi
Name:crp
Synonyms:cap
Ordered Locus Names:HI_0957
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Loss of competence, the ability to bind, take-up and integrate exogenous DNA. Loss of the ability to ferment ribose or xylose.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001471 – 224cAMP-activated global transcriptional regulator CRPAdd BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei115N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Expressioni

Inductioni

Constitutively expressed; slightly induced on shifting to starvation media.1 Publication

Interactioni

Subunit structurei

Homodimer, which upon binding cAMP is able to bind DNA (Probable). Binds RNA polymerase subunit RpoA (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi71421.HI0957.

Structurei

3D structure databases

ProteinModelPortaliP29281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini152 – 224HTH crp-typePROSITE-ProRule annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 36Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity3
Regioni67 – 73Activating region 3 (AR3); probably contacts sigma-70 (RpoD)By similarity7
Regioni168 – 177Activating region 1 (AR1); probably contacts the C-terminus of RpoABy similarity10

Domaini

The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107RYR. Bacteria.
COG0664. LUCA.
KOiK10914.
OMAiIMARRLR.
PhylomeDBiP29281.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29281-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNELTEIDE VVTSSQEEAT QRDPVLDWFL THCHLHKYPA KSTLIHAGED
60 70 80 90 100
ATTLYYVIKG SVMVSSKDDE GKEMILTYLG AGQFFGEAGL FDEGSKRSAW
110 120 130 140 150
VKTKTTCEIA EISYKKYRQL IQANPEILMF LTAQLARRLQ NTSRQVTNLA
160 170 180 190 200
FLDVAGRIAQ TLMNLAKQPE AMTHPDGMQI KITRQEIGQM VGCSRETVGR
210 220
IIKMLEDQNL IHAHGKTIVV YGAR
Length:224
Mass (Da):25,152
Last modified:December 1, 1992 - v1
Checksum:i421B7790A48FF50C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77207 Genomic DNA. Translation: AAA24952.1.
L42023 Genomic DNA. Translation: AAC22618.1.
PIRiB38225.
RefSeqiNP_439118.1. NC_000907.1.
WP_005647998.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22618; AAC22618; HI_0957.
GeneIDi949963.
KEGGihin:HI0957.
PATRICi20190573. VBIHaeInf48452_0999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77207 Genomic DNA. Translation: AAA24952.1.
L42023 Genomic DNA. Translation: AAC22618.1.
PIRiB38225.
RefSeqiNP_439118.1. NC_000907.1.
WP_005647998.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP29281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22618; AAC22618; HI_0957.
GeneIDi949963.
KEGGihin:HI0957.
PATRICi20190573. VBIHaeInf48452_0999.

Phylogenomic databases

eggNOGiENOG4107RYR. Bacteria.
COG0664. LUCA.
KOiK10914.
OMAiIMARRLR.
PhylomeDBiP29281.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRP_HAEIN
AccessioniPrimary (citable) accession number: P29281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 5, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.