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P29281

- CRP_HAEIN

UniProt

P29281 - CRP_HAEIN

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Protein

cAMP-activated global transcriptional regulator CRP

Gene

crp

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

A global transcription regulator required for bacterial competence. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to regulate transcription at 54 promoters (PubMed:15769466). There are 2 consensus subclasses; CRP-N (above) and CRP-S (T6 changed to C and A17 changed to G) which obligatorily also requires Sxy for expression. CRP-S sites bind DNA but do not activate transcription in the absence of Sxy. Probably induces a severe bend in DNA. Probably acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei111 – 1111Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
Sitei116 – 1161Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 777cAMP 1By similarity
Nucleotide bindingi86 – 883cAMP 1By similarity
Nucleotide bindingi97 – 982cAMP 1By similarity
Nucleotide bindingi142 – 1432cAMP 1By similarity
Nucleotide bindingi150 – 1512cAMP 2By similarity
Nucleotide bindingi185 – 19511cAMP 2By similarityAdd
BLAST
DNA bindingi194 – 2007H-T-H motifPROSITE-ProRule annotation

GO - Molecular functioni

  1. cAMP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. establishment of competence for transformation Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Competence, Transcription, Transcription regulation

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-activated global transcriptional regulator CRP
Alternative name(s):
Catabolite activator protein
Short name:
CAP
cAMP receptor protein
Short name:
CRP
cAMP regulatory protein
Gene namesi
Name:crp
Synonyms:cap
Ordered Locus Names:HI_0957
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Loss of competence, the ability to bind, take-up and integrate exogenous DNA. Loss of the ability to ferment ribose or xylose.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224cAMP-activated global transcriptional regulator CRPPRO_0000100147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei115 – 1151N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Expressioni

Inductioni

Constitutively expressed; slightly induced on shifting to starvation media.1 Publication

Interactioni

Subunit structurei

Homodimer, which upon binding cAMP is able to bind DNA (Probable). Binds RNA polymerase subunit RpoA (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi71421.HI0957.

Structurei

3D structure databases

ProteinModelPortaliP29281.
SMRiP29281. Positions 21-222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 22473HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 363Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity
Regioni67 – 737Activating region 3 (AR3); probably contacts sigma-70 (RpoD)By similarity
Regioni168 – 17710Activating region 1 (AR1); probably contacts the C-terminus of RpoABy similarity

Domaini

The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664.
KOiK10914.
OMAiINCTHED.
OrthoDBiEOG69GZGV.
PhylomeDBiP29281.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29281-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNELTEIDE VVTSSQEEAT QRDPVLDWFL THCHLHKYPA KSTLIHAGED
60 70 80 90 100
ATTLYYVIKG SVMVSSKDDE GKEMILTYLG AGQFFGEAGL FDEGSKRSAW
110 120 130 140 150
VKTKTTCEIA EISYKKYRQL IQANPEILMF LTAQLARRLQ NTSRQVTNLA
160 170 180 190 200
FLDVAGRIAQ TLMNLAKQPE AMTHPDGMQI KITRQEIGQM VGCSRETVGR
210 220
IIKMLEDQNL IHAHGKTIVV YGAR
Length:224
Mass (Da):25,152
Last modified:December 1, 1992 - v1
Checksum:i421B7790A48FF50C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77207 Genomic DNA. Translation: AAA24952.1.
L42023 Genomic DNA. Translation: AAC22618.1.
PIRiB38225.
RefSeqiNP_439118.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22618; AAC22618; HI_0957.
GeneIDi949963.
KEGGihin:HI0957.
PATRICi20190573. VBIHaeInf48452_0999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77207 Genomic DNA. Translation: AAA24952.1 .
L42023 Genomic DNA. Translation: AAC22618.1 .
PIRi B38225.
RefSeqi NP_439118.1. NC_000907.1.

3D structure databases

ProteinModelPortali P29281.
SMRi P29281. Positions 21-222.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22618 ; AAC22618 ; HI_0957 .
GeneIDi 949963.
KEGGi hin:HI0957.
PATRICi 20190573. VBIHaeInf48452_0999.

Phylogenomic databases

eggNOGi COG0664.
KOi K10914.
OMAi INCTHED.
OrthoDBi EOG69GZGV.
PhylomeDBi P29281.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view ]
PRINTSi PR00034. HTHCRP.
SMARTi SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding cAMP receptor protein is required for competence development in Haemophilus influenzae Rd."
    Chandler M.S.
    Proc. Natl. Acad. Sci. U.S.A. 89:1626-1630(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PROBABLE AUTOREGULATION, DISRUPTION PHENOTYPE.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  3. "The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence."
    Dorocicz I.R., Williams P.M., Redfield R.J.
    J. Bacteriol. 175:7142-7149(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  4. "A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae."
    Redfield R.J., Cameron A.D., Qian Q., Hinds J., Ali T.R., Kroll J.S., Langford P.R.
    J. Mol. Biol. 347:735-747(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULON, INDUCTION.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  5. "CRP binding and transcription activation at CRP-S sites."
    Cameron A.D., Redfield R.J.
    J. Mol. Biol. 383:313-323(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiCRP_HAEIN
AccessioniPrimary (citable) accession number: P29281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 1, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3