P29275 (AA2BR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine receptor A2b

Gene names

Name:

ADORA2B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement Ref.2. Source: ProtInc JNK cascade Traceable author statement PubMed 10101031. Source: ProtInc activation of MAPK activity Traceable author statement PubMed 10101031. Source: ProtInc activation of adenylate cyclase activity Traceable author statement PubMed 8022409. Source: ProtInc adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara cellular defense response Traceable author statement PubMed 10101031. Source: ProtInc cellular response to extracellular stimulus Inferred from electronic annotation. Source: Compara excretion Traceable author statement PubMed 7836474. Source: ProtInc positive regulation of cGMP biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of chemokine production Inferred from electronic annotation. Source: Compara positive regulation of chronic inflammatory response to non-antigenic stimulus Inferred from electronic annotation. Source: Compara positive regulation of guanylate cyclase activity Inferred from electronic annotation. Source: Compara positive regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara positive regulation of mast cell degranulation Inferred from electronic annotation. Source: Compara positive regulation vascular endothelial growth factor production Inferred from electronic annotation. Source: Compara relaxation of vascular smooth muscle Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	G-protein coupled adenosine receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Adenosine receptor A2b		PRO_0000069003
Regions
	Topological domain	1 – 8	8	Extracellular By similarity
	Transmembrane	9 – 33	25	Helical; Name=1; By similarity
	Topological domain	34 – 43	10	Cytoplasmic By similarity
	Transmembrane	44 – 67	24	Helical; Name=2; By similarity
	Topological domain	68 – 78	11	Extracellular By similarity
	Transmembrane	79 – 101	23	Helical; Name=3; By similarity
	Topological domain	102 – 121	20	Cytoplasmic By similarity
	Transmembrane	122 – 144	23	Helical; Name=4; By similarity
	Topological domain	145 – 178	34	Extracellular By similarity
	Transmembrane	179 – 203	25	Helical; Name=5; By similarity
	Topological domain	204 – 235	32	Cytoplasmic By similarity
	Transmembrane	236 – 259	24	Helical; Name=6; By similarity
	Topological domain	260 – 267	8	Extracellular By similarity
	Transmembrane	268 – 291	24	Helical; Name=7; By similarity
	Topological domain	292 – 332	41	Cytoplasmic By similarity
	Region	173 – 182	10	Agonist binding By similarity
	Region	247 – 254	8	Agonist binding By similarity
	Region	266 – 276	11	Agonist binding By similarity
Amino acid modifications
	Lipidation	311	1	S-palmitoyl cysteine Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Disulfide bond	78 ↔ 171		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29275 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 38AD088E2439C7FA	FASTA	332	36,333
	10 20 30 40 50 60 MLLETQDALY VALELVIAAL SVAGNVLVCA AVGTANTLQT PTNYFLVSLA AADVAVGLFA 70 80 90 100 110 120 IPFAITISLG FCTDFYGCLF LACFVLVLTQ SSIFSLLAVA VDRYLAICVP LRYKSLVTGT 130 140 150 160 170 180 RARGVIAVLW VLAFGIGLTP FLGWNSKDSA TNNCTEPWDG TTNESCCLVK CLFENVVPMS 190 200 210 220 230 240 YMVYFNFFGC VLPPLLIMLV IYIKIFLVAC RQLQRTELMD HSRTTLQREI HAAKSLAMIV 250 260 270 280 290 300 GIFALCWLPV HAVNCVTLFQ PAQGKNKPKW AMNMAILLSH ANSVVNPIVY AYRNRDFRYT 310 320 330 FHKIISRYLL CQADVKSGNG QAGVQPALGV GL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of an adenosine A2b receptor from human brain." Pierce K.D., Furlong T.J., Selbie L.A., Shine J. Biochem. Biophys. Res. Commun. 187:86-93(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Cloning and chromosomal localization of the human A2b adenosine receptor gene (ADORA2B) and its pseudogene." Jacobson M.A., Johnson R.G., Luneau C.J., Salvatore C.A. Genomics 27:374-376(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M97759 mRNA. Translation: AAA51598.1. X68487 mRNA. Translation: CAA48505.1. AY136748 mRNA. Translation: AAN01274.1. BC025722 mRNA. Translation: AAH25722.1.
IPI	IPI00020976.
PIR	JC1229.
RefSeq	NP_000667.1. NM_000676.2.
UniGene	Hs.167046.
3D structure databases
ProteinModelPortal	P29275.
ModBase	Search...
Protein-protein interaction databases
IntAct	P29275. 2 interactions.
STRING	9606.ENSP00000304501.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P29275.
Polymorphism databases
DMDM	112938.
Proteomic databases
PaxDb	P29275.
PRIDE	P29275.
Protocols and materials databases
DNASU	136.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000304222; ENSP00000304501; ENSG00000170425.
GeneID	136.
KEGG	hsa:136.
UCSC	uc002gpd.1. human.
Organism-specific databases
CTD	136.
GeneCards	GC17P015788.
HGNC	HGNC:264. ADORA2B.
MIM	600446. gene.
neXtProt	NX_P29275.
PharmGKB	PA24585.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG287824.
HOGENOM	HOG000015770.
HOVERGEN	HBG106962.
InParanoid	P29275.
KO	K04267.
OMA	VIYIKIF.
OrthoDB	EOG4FBHTB.
PhylomeDB	P29275.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P29275.
Bgee	P29275.
CleanEx	HS_ADORA2B.
Genevestigator	P29275.
GermOnline	ENSG00000170425. Homo sapiens.
Family and domain databases
InterPro	IPR001435. Adeno_A2B_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00554. ADENOSINA2BR. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P29275.
ChEMBL	CHEMBL255.
ChiTaRS	ADORA2B. human.
DrugBank	DB04932. Defibrotide. DB00824. Enprofylline. DB00061. Pegademase bovine. DB00277. Theophylline.
GenomeRNAi	136.
NextBio	547.
SOURCE	Search...

Entry information

Entry name

AA2BR_HUMAN

Accession

Primary (citable) accession number: P29275

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.