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Reviewed, UniProtKB/Swiss-Prot P29274 (AA2AR_HUMAN)

Last modified November 3, 2009. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Adenosine receptor A2a
Gene names
Name: ADORA2A
Synonyms: ADORA2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processG-protein signaling, coupled to cAMP nucleotide second messenger

Traceable author statement. Source: ProtInc

activation of adenylate cyclase activity

Inferred from Experiment. Source: Reactome

apoptosis

Traceable author statement. Source: ProtInc

blood circulation

Traceable author statement. Source: ProtInc

blood coagulation

Traceable author statement. Source: ProtInc

cAMP biosynthetic process

Traceable author statement. Source: ProtInc

cell-cell signaling

Traceable author statement. Source: ProtInc

cellular defense response

Traceable author statement. Source: ProtInc

central nervous system development

Traceable author statement. Source: ProtInc

inflammatory response

Traceable author statement. Source: ProtInc

phagocytosis

Traceable author statement. Source: ProtInc

sensory perception

Traceable author statement. Source: ProtInc

transmembrane receptor protein tyrosine kinase signaling pathway

Inferred from Experiment. Source: Reactome

   Cellular componentintegral to plasma membrane

Traceable author statement. Source: ProtInc

membrane fraction

Traceable author statement. Source: ProtInc

   Molecular functionadenosine receptor activity, G-protein coupled

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Adenosine receptor A2a
PRO_0000068999

Regions

Topological domain1 – 77Extracellular Ref.12
Transmembrane8 – 32251
Topological domain33 – 4210Cytoplasmic Ref.12
Transmembrane43 – 66242
Topological domain67 – 7711Extracellular Ref.12
Transmembrane78 – 100233
Topological domain101 – 12020Cytoplasmic Ref.12
Transmembrane121 – 143234
Topological domain144 – 17330Extracellular Ref.12
Transmembrane174 – 198255
Topological domain199 – 23436Cytoplasmic Ref.12
Transmembrane235 – 258246
Topological domain259 – 2668Extracellular Ref.12
Transmembrane267 – 290247
Topological domain291 – 412122Cytoplasmic Ref.12
Region168 – 17710Agonist binding
Region246 – 2538Agonist binding
Region264 – 27411Agonist binding

Amino acid modifications

Glycosylation1541N-linked (GlcNAc...) Potential
Disulfide bond71 ↔ 159 Ref.12
Disulfide bond74 ↔ 146 Ref.12
Disulfide bond77 ↔ 166 Ref.12
Disulfide bond259 ↔ 262 Ref.12

Natural variations

Natural variant501A → V: dbSNP rs4530. Ref.13
VAR_011835
Natural variant3001R → H: dbSNP rs4990.
VAR_011836
Natural variant3921G → R
VAR_003451

Secondary structure

....................................... 412
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29274-1 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 9438E9D64A6BE61B

FASTA41244,707
        10         20         30         40         50         60 
MPIMGSSVYI TVELAIAVLA ILGNVLVCWA VWLNSNLQNV TNYFVVSLAA ADIAVGVLAI 

        70         80         90        100        110        120 
PFAITISTGF CAACHGCLFI ACFVLVLTQS SIFSLLAIAI DRYIAIRIPL RYNGLVTGTR 

       130        140        150        160        170        180 
AKGIIAICWV LSFAIGLTPM LGWNNCGQPK EGKNHSQGCG EGQVACLFED VVPMNYMVYF 

       190        200        210        220        230        240 
NFFACVLVPL LLMLGVYLRI FLAARRQLKQ MESQPLPGER ARSTLQKEVH AAKSLAIIVG 

       250        260        270        280        290        300 
LFALCWLPLH IINCFTFFCP DCSHAPLWLM YLAIVLSHTN SVVNPFIYAY RIREFRQTFR 

       310        320        330        340        350        360 
KIIRSHVLRQ QEPFKAAGTS ARVLAAHGSD GEQVSLRLNG HPPGVWANGS APHPERRPNG 

       370        380        390        400        410 
YALGLVSGGS AQESQGNTGL PDVELLSHEL KGVCPEPPGL DDPLAQDGAG VS 

« Hide

References

« Hide 'large scale' references
[1]Tiffany H.L., Murphy P.M.
Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Salvatore C.A., Luneau C.J., Johnson R.G., Jacobson M.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Molecular characterization of a human brain adenosine A2 receptor."
Furlong T.J., Pierce K.D., Selbie L.A., Shine J.
Brain Res. Mol. Brain Res. 15:62-66(1992) [PubMed: 1331670] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Hippocampus.
[4]"Characterization and chromosomal localization of the human A2a adenosine receptor gene: ADORA2A."
Le F., Townsend-Nicholson A., Baker E., Sutherland G.R., Schofield P.R.
Biochem. Biophys. Res. Commun. 223:461-467(1996) [PubMed: 8670304] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[9]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lymph.
[11]"Site-directed mutagenesis identifies residues involved in ligand recognition in the human A2a adenosine receptor."
Kim J., Wess J., van Rhee A.M., Schoneberg T., Jacobson K.A.
J. Biol. Chem. 270:13987-13997(1995) [PubMed: 7775460] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF TRANSMEMBRANE DOMAINS.
[12]"The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist."
Jaakola V.-P., Griffith M.T., Hanson M.A., Cherezov V., Chien E.Y.T., Lane J.R., Ijzerman A.P., Stevens R.C.
Science 322:1211-1217(2008) [PubMed: 18832607] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 2-316 IN COMPLEX WITH ANTAGONIST, TOPOLOGY, DISULFIDE BONDS.
[13]"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 22:231-238(1999) [PubMed: 10391209] [Abstract]
Cited for: VARIANT VAL-50.
[14]Erratum
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 23:373-373(1999)
+Additional computationally mapped references.

Cross-references

Sequence databases

M97370 mRNA. Translation: AAA58356.1. Different initiation.
X68486 mRNA. Translation: CAA48504.1.
S46950 mRNA. Translation: AAB23956.1.
U40771, U40770 Genomic DNA. Translation: AAA83270.1.
AY136747 mRNA. Translation: AAN01273.1.
CR456367 mRNA. Translation: CAG30253.1.
BT006999 mRNA. Translation: AAP35645.1.
AK312946 mRNA. Translation: BAG35787.1.
CH471095 Genomic DNA. Translation: EAW59658.1.
BC013780 mRNA. Translation: AAH13780.1.
IPIIPI00020974.
PIRA48978.
RefSeqNP_000666.2.
UniGeneHs.197029
Hs.712771

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1MMHmodel-16-36[»]
242-69[»]
378-102[»]
4117-143[»]
5175-203[»]
6233-260[»]
7264-296[»]
1UPEmodel-A1-304[»]
3EMLX-ray2.60A2-316[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP29274.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP29274.

Proteomic databases

PRIDEP29274.

Genome annotation databases

EnsemblENST00000337539; ENSP00000336630; ENSG00000128271; Homo sapiens. [Genome view]
ENST00000406084; ENSP00000385337; ENSG00000128271; Homo sapiens. [Genome view]
ENST00000417596; ENSP00000394368; ENSG00000128271; Homo sapiens. [Genome view]
ENST00000424232; ENSP00000404497; ENSG00000128271; Homo sapiens. [Genome view]
ENST00000436735; ENSP00000397071; ENSG00000128271; Homo sapiens. [Genome view]
ENST00000439591; ENSP00000400190; ENSG00000128271; Homo sapiens. [Genome view]
ENST00000444262; ENSP00000414802; ENSG00000128271; Homo sapiens. [Genome view]
GeneID135.
KEGGhsa:135.
UCSCuc002zzx.1. human.

Organism-specific databases

CTD135.
GeneCardsGC22P022997.
HGNCHGNC:263. ADORA2A.
MIM102776. gene.
PharmGKBPA24584.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP29274.
HOVERGENP29274.
OMACSHAPLW.

Enzyme and pathway databases

ReactomeREACT_11061. Signalling by NGF.
REACT_14797. Signaling by GPCR.

Gene expression databases

ArrayExpressP29274.
BgeeP29274.
CleanExHS_ADORA2A.
GenevestigatorP29274.
GermOnlineENSG00000128271. Homo sapiens.

Family and domain databases

InterProIPR000276. 7TM_GPCR_Rhodpsn.
IPR001513. Adeno_A2A_rcpt.
IPR001634. Adenosn_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00553. ADENOSINA2AR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00201. Caffeine.
DB04932. Defibrotide.
DB00061. Pegademase bovine.
DB00277. Theophylline.
NextBio543.
SOURCESearch...

Entry information

Entry nameAA2AR_HUMAN
AccessionPrimary (citable) accession number: P29274
Secondary accession number(s): B2R7E0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: November 3, 2009
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents