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Protein

Connective tissue growth factor

Gene

Ctgf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).By similarity2 Publications

GO - Molecular functioni

  • fibronectin binding Source: Ensembl
  • growth factor activity Source: Ensembl
  • heparin binding Source: MGI
  • insulin-like growth factor binding Source: InterPro
  • integrin binding Source: MGI
  • protein C-terminus binding Source: MGI

GO - Biological processi

  • aging Source: Ensembl
  • angiogenesis Source: MGI
  • cartilage condensation Source: MGI
  • cell-cell signaling Source: GO_Central
  • cell differentiation Source: MGI
  • cell-matrix adhesion Source: MGI
  • cell migration Source: MGI
  • chondrocyte proliferation Source: MGI
  • connective tissue development Source: MGI
  • cytosolic calcium ion transport Source: Ensembl
  • DNA biosynthetic process Source: UniProtKB-KW
  • extracellular matrix constituent secretion Source: Ensembl
  • fibroblast growth factor receptor signaling pathway Source: MGI
  • integrin-mediated signaling pathway Source: MGI
  • intracellular signal transduction Source: Ensembl
  • lung development Source: MGI
  • negative regulation of cell death Source: GO_Central
  • negative regulation of gene expression Source: MGI
  • ossification Source: MGI
  • positive regulation of cardiac muscle contraction Source: Ensembl
  • positive regulation of cell activation Source: Ensembl
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of cell proliferation Source: Ensembl
  • positive regulation of collagen biosynthetic process Source: Ensembl
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • positive regulation of G0 to G1 transition Source: Ensembl
  • positive regulation of gene expression Source: Ensembl
  • positive regulation of JNK cascade Source: MGI
  • positive regulation of stress fiber assembly Source: MGI
  • reactive oxygen species metabolic process Source: MGI
  • regulation of cell growth Source: InterPro
  • regulation of chondrocyte differentiation Source: MGI
  • response to amino acid Source: Ensembl
  • response to anoxia Source: Ensembl
  • response to estradiol Source: Ensembl
  • response to fatty acid Source: Ensembl
  • response to glucose Source: Ensembl
  • response to mineralocorticoid Source: Ensembl
  • response to peptide hormone Source: Ensembl
  • tissue homeostasis Source: MGI

Keywordsi

Molecular functionHeparin-binding
Biological processCell adhesion, DNA synthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Connective tissue growth factor
Alternative name(s):
CCN family member 2
Hypertrophic chondrocyte-specific protein 24
Protein FISP-12
Gene namesi
Name:Ctgf
Synonyms:Ccn2, Fisp-12, Fisp12, Hcs24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:95537. Ctgf.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001440326 – 348Connective tissue growth factorAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi255 ↔ 292By similarity
Disulfide bondi272 ↔ 306By similarity
Disulfide bondi283 ↔ 322By similarity
Disulfide bondi286 ↔ 324By similarity
Disulfide bondi291 ↔ 328By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP29268.
PaxDbiP29268.
PeptideAtlasiP29268.
PRIDEiP29268.

PTM databases

PhosphoSitePlusiP29268.

Expressioni

Tissue specificityi

Testis, spleen, kidney, lung, heart, and brain (lowest level in testis and highest in lung).

Inductioni

By growth factors.

Gene expression databases

BgeeiENSMUSG00000019997.
CleanExiMM_CTGF.
ExpressionAtlasiP29268. baseline and differential.
GenevisibleiP29268. MM.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP29268. 1 interactor.
STRINGi10090.ENSMUSP00000020171.

Structurei

3D structure databases

ProteinModelPortaliP29268.
SMRiP29268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 97IGFBP N-terminalPROSITE-ProRule annotationAdd BLAST72
Domaini100 – 166VWFCPROSITE-ProRule annotationAdd BLAST67
Domaini197 – 242TSP type-1PROSITE-ProRule annotationAdd BLAST46
Domaini255 – 329CTCKPROSITE-ProRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 348Heparin-bindingBy similarityAdd BLAST103

Sequence similaritiesi

Belongs to the CCN family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IH0W. Eukaryota.
ENOG4111F77. LUCA.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiP29268.
KOiK06827.
OMAiCTPHRTA.
OrthoDBiEOG091G0SQP.
TreeFamiTF326070.

Family and domain databases

InterProiView protein in InterPro
IPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. TSP1_rpt.
IPR001007. VWF_dom.
PfamiView protein in Pfam
PF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiView protein in SMART
SM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiView protein in PROSITE
PS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASVAGPIS LALVLLALCT RPAMGQDCSA QCQCAAEAAP HCPAGVSLVL
60 70 80 90 100
DGCGCCRVCA KQLGELCTER DPCDPHKGLF CDFGSPANRK IGVCTAKDGA
110 120 130 140 150
PCVFGGSVYR SGESFQSSCK YQCTCLDGAV GCVPLCSMDV RLPSPDCPFP
160 170 180 190 200
RRVKLPGKCC EEWVCDEPKD RTAVGPALAA YRLEDTFGPD PTMMRANCLV
210 220 230 240 250
QTTEWSACSK TCGMGISTRV TNDNTFCRLE KQSRLCMVRP CEADLEENIK
260 270 280 290 300
KGKKCIRTPK IAKPVKFELS GCTSVKTYRA KFCGVCTDGR CCTPHRTTTL
310 320 330 340
PVEFKCPDGE IMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
Length:348
Mass (Da):37,824
Last modified:October 3, 2012 - v3
Checksum:i9BD99E6D0BEFC7BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24M → T in AAA37627 (PubMed:1888698).Curated1
Sequence conflicti24M → T in AAA37628 (PubMed:1888698).Curated1
Sequence conflicti24M → T in AAA73135 (PubMed:2029337).Curated1
Sequence conflicti24M → T in AAH06783 (PubMed:15489334).Curated1
Sequence conflicti161E → K in AAA37627 (PubMed:1888698).Curated1
Sequence conflicti161E → K in AAA37628 (PubMed:1888698).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M70641 Genomic DNA. Translation: AAA37627.1.
M70642 mRNA. Translation: AAA37628.1.
M80263 mRNA. Translation: AAA73135.1.
AC099695 Genomic DNA. No translation available.
CH466540 Genomic DNA. Translation: EDL04783.1.
BC006783 mRNA. Translation: AAH06783.1.
CCDSiCCDS23751.1.
PIRiA40578.
RefSeqiNP_034347.2. NM_010217.2.
UniGeneiMm.390287.

Genome annotation databases

EnsembliENSMUST00000020171; ENSMUSP00000020171; ENSMUSG00000019997.
GeneIDi14219.
KEGGimmu:14219.
UCSCiuc011xbr.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCTGF_MOUSE
AccessioniPrimary (citable) accession number: P29268
Secondary accession number(s): G5E830, Q922U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 3, 2012
Last modified: September 27, 2017
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families