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Protein

Connective tissue growth factor

Gene

Ctgf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).By similarity

GO - Molecular functioni

  1. heparin binding Source: MGI
  2. integrin binding Source: MGI

GO - Biological processi

  1. angiogenesis Source: MGI
  2. cartilage condensation Source: MGI
  3. cell-cell signaling Source: GO_Central
  4. cell differentiation Source: MGI
  5. cell-matrix adhesion Source: MGI
  6. cell migration Source: MGI
  7. chondrocyte proliferation Source: MGI
  8. connective tissue development Source: MGI
  9. cytosolic calcium ion transport Source: Ensembl
  10. DNA biosynthetic process Source: UniProtKB-KW
  11. extracellular matrix constituent secretion Source: Ensembl
  12. fibroblast growth factor receptor signaling pathway Source: MGI
  13. integrin-mediated signaling pathway Source: MGI
  14. intracellular signal transduction Source: Ensembl
  15. lung development Source: MGI
  16. negative regulation of cell death Source: GO_Central
  17. negative regulation of gene expression Source: MGI
  18. organ senescence Source: Ensembl
  19. ossification Source: MGI
  20. positive regulation of cardiac muscle contraction Source: Ensembl
  21. positive regulation of cell activation Source: Ensembl
  22. positive regulation of cell differentiation Source: MGI
  23. positive regulation of cell proliferation Source: Ensembl
  24. positive regulation of collagen biosynthetic process Source: Ensembl
  25. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  26. positive regulation of ERK1 and ERK2 cascade Source: MGI
  27. positive regulation of G0 to G1 transition Source: Ensembl
  28. positive regulation of gene expression Source: Ensembl
  29. positive regulation of JNK cascade Source: MGI
  30. positive regulation of protein phosphorylation Source: Ensembl
  31. positive regulation of stress fiber assembly Source: MGI
  32. reactive oxygen species metabolic process Source: MGI
  33. regulation of cell growth Source: InterPro
  34. regulation of chondrocyte differentiation Source: MGI
  35. response to amino acid Source: Ensembl
  36. response to anoxia Source: Ensembl
  37. response to estradiol Source: Ensembl
  38. response to fatty acid Source: Ensembl
  39. response to glucose Source: Ensembl
  40. response to mineralocorticoid Source: Ensembl
  41. response to peptide hormone Source: Ensembl
  42. tissue homeostasis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, DNA synthesis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_198602. PPARA activates gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Connective tissue growth factor
Alternative name(s):
CCN family member 2
Hypertrophic chondrocyte-specific protein 24
Protein FISP-12
Gene namesi
Name:Ctgf
Synonyms:Ccn2, Fisp-12, Fisp12, Hcs24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:95537. Ctgf.

Subcellular locationi

Secretedextracellular spaceextracellular matrix 1 Publication. Secreted 1 Publication

GO - Cellular componenti

  1. cell cortex Source: Ensembl
  2. cis-Golgi network Source: Ensembl
  3. cytosol Source: Ensembl
  4. extracellular region Source: MGI
  5. extracellular space Source: Ensembl
  6. Golgi apparatus Source: MGI
  7. intracellular membrane-bounded organelle Source: MGI
  8. perinuclear region of cytoplasm Source: Ensembl
  9. proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 348323Connective tissue growth factorPRO_0000014403Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi255 ↔ 292By similarity
Disulfide bondi272 ↔ 306By similarity
Disulfide bondi283 ↔ 322By similarity
Disulfide bondi286 ↔ 324By similarity
Disulfide bondi291 ↔ 328By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP29268.
PaxDbiP29268.
PRIDEiP29268.

PTM databases

PhosphoSiteiP29268.

Expressioni

Tissue specificityi

Testis, spleen, kidney, lung, heart, and brain (lowest level in testis and highest in lung).

Inductioni

By growth factors.

Gene expression databases

CleanExiMM_CTGF.
ExpressionAtlasiP29268. baseline and differential.
GenevestigatoriP29268.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020171.

Structurei

3D structure databases

ProteinModelPortaliP29268.
SMRiP29268. Positions 39-135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 9772IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini100 – 16667VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini197 – 24246TSP type-1PROSITE-ProRule annotationAdd
BLAST
Domaini255 – 32975CTCKPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni246 – 348103Heparin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CCN family.Curated
Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG72934.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiP29268.
KOiK06827.
OMAiTYRAKFC.
OrthoDBiEOG7N8ZXB.
TreeFamiTF326070.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASVAGPIS LALVLLALCT RPAMGQDCSA QCQCAAEAAP HCPAGVSLVL
60 70 80 90 100
DGCGCCRVCA KQLGELCTER DPCDPHKGLF CDFGSPANRK IGVCTAKDGA
110 120 130 140 150
PCVFGGSVYR SGESFQSSCK YQCTCLDGAV GCVPLCSMDV RLPSPDCPFP
160 170 180 190 200
RRVKLPGKCC EEWVCDEPKD RTAVGPALAA YRLEDTFGPD PTMMRANCLV
210 220 230 240 250
QTTEWSACSK TCGMGISTRV TNDNTFCRLE KQSRLCMVRP CEADLEENIK
260 270 280 290 300
KGKKCIRTPK IAKPVKFELS GCTSVKTYRA KFCGVCTDGR CCTPHRTTTL
310 320 330 340
PVEFKCPDGE IMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
Length:348
Mass (Da):37,824
Last modified:October 3, 2012 - v3
Checksum:i9BD99E6D0BEFC7BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241M → T in AAA37627 (PubMed:1888698).Curated
Sequence conflicti24 – 241M → T in AAA37628 (PubMed:1888698).Curated
Sequence conflicti24 – 241M → T in AAA73135 (PubMed:2029337).Curated
Sequence conflicti24 – 241M → T in AAH06783 (PubMed:15489334).Curated
Sequence conflicti161 – 1611E → K in AAA37627 (PubMed:1888698).Curated
Sequence conflicti161 – 1611E → K in AAA37628 (PubMed:1888698).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M70641 Genomic DNA. Translation: AAA37627.1.
M70642 mRNA. Translation: AAA37628.1.
M80263 mRNA. Translation: AAA73135.1.
AC099695 Genomic DNA. No translation available.
CH466540 Genomic DNA. Translation: EDL04783.1.
BC006783 mRNA. Translation: AAH06783.1.
CCDSiCCDS23751.1.
PIRiA40578.
RefSeqiNP_034347.2. NM_010217.2.
UniGeneiMm.390287.

Genome annotation databases

EnsembliENSMUST00000020171; ENSMUSP00000020171; ENSMUSG00000019997.
GeneIDi14219.
KEGGimmu:14219.
UCSCiuc011xbr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M70641 Genomic DNA. Translation: AAA37627.1.
M70642 mRNA. Translation: AAA37628.1.
M80263 mRNA. Translation: AAA73135.1.
AC099695 Genomic DNA. No translation available.
CH466540 Genomic DNA. Translation: EDL04783.1.
BC006783 mRNA. Translation: AAH06783.1.
CCDSiCCDS23751.1.
PIRiA40578.
RefSeqiNP_034347.2. NM_010217.2.
UniGeneiMm.390287.

3D structure databases

ProteinModelPortaliP29268.
SMRiP29268. Positions 39-135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020171.

PTM databases

PhosphoSiteiP29268.

Proteomic databases

MaxQBiP29268.
PaxDbiP29268.
PRIDEiP29268.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020171; ENSMUSP00000020171; ENSMUSG00000019997.
GeneIDi14219.
KEGGimmu:14219.
UCSCiuc011xbr.1. mouse.

Organism-specific databases

CTDi1490.
MGIiMGI:95537. Ctgf.

Phylogenomic databases

eggNOGiNOG72934.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiP29268.
KOiK06827.
OMAiTYRAKFC.
OrthoDBiEOG7N8ZXB.
TreeFamiTF326070.

Enzyme and pathway databases

ReactomeiREACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_198602. PPARA activates gene expression.

Miscellaneous databases

NextBioi285469.
PROiP29268.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CTGF.
ExpressionAtlasiP29268. baseline and differential.
GenevestigatoriP29268.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, mapping, and expression of fisp-12, a growth factor-inducible gene encoding a secreted cysteine-rich protein."
    Ryseck R.-P., Macdonald-Bravo H., Mattei M.-G., Bravo R.
    Cell Growth Differ. 2:225-233(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "Identification of a gene family regulated by transforming growth factor-beta."
    Brunner A., Chinn J., Neubauer M.G., Purchio A.F.
    DNA Cell Biol. 10:293-300(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Cyr61 and Fisp12 are both ECM-associated signaling molecules: activities, metabolism, and localization during development."
    Kireeva M.L., Latinkic B.V., Kolesnikova T.V., Chen C.-C., Yang G.P., Abler A.S., Lau L.F.
    Exp. Cell Res. 233:63-77(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and migration through integrin alphavbeta3, promotes endothelial cell survival, and induces angiogenesis in vivo."
    Babic A.M., Chen C.-C., Lau L.F.
    Mol. Cell. Biol. 19:2958-2966(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCTGF_MOUSE
AccessioniPrimary (citable) accession number: P29268
Secondary accession number(s): G5E830, Q922U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 3, 2012
Last modified: February 4, 2015
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.