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P29268 (CTGF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Connective tissue growth factor
Alternative name(s):
CCN family member 2
Hypertrophic chondrocyte-specific protein 24
Protein FISP-12
Gene names
Name:Ctgf
Synonyms:Ccn2, Fisp-12, Fisp12, Hcs24
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes By similarity. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells By similarity. Enhances fibroblast growth factor-induced DNA synthesis By similarity. Ref.6 Ref.7

Subunit structure

Monomer By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix. Secreted Ref.6.

Tissue specificity

Testis, spleen, kidney, lung, heart, and brain (lowest level in testis and highest in lung).

Induction

By growth factors.

Sequence similarities

Belongs to the CCN family.

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Contains 1 IGFBP N-terminal domain.

Contains 1 TSP type-1 domain.

Contains 1 VWFC domain.

Ontologies

Keywords
   Biological processCell adhesion
DNA synthesis
   Cellular componentExtracellular matrix
Secreted
   DomainSignal
   LigandHeparin-binding
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

angiogenesis

Inferred from direct assay Ref.7PubMed 10446209. Source: MGI

cartilage condensation

Inferred from direct assay PubMed 15305294. Source: MGI

cell differentiation

Inferred from direct assay PubMed 15305294. Source: MGI

cell migration

Inferred from direct assay Ref.7. Source: MGI

cell-matrix adhesion

Inferred from direct assay Ref.6. Source: MGI

chondrocyte proliferation

Inferred from mutant phenotype PubMed 18597638. Source: MGI

connective tissue development

Inferred from mutant phenotype PubMed 23839921. Source: MGI

cytosolic calcium ion transport

Inferred from electronic annotation. Source: Ensembl

extracellular matrix constituent secretion

Inferred from electronic annotation. Source: Ensembl

fibroblast growth factor receptor signaling pathway

Inferred from direct assay Ref.6. Source: MGI

integrin-mediated signaling pathway

Inferred from direct assay Ref.7PubMed 10446209. Source: MGI

intracellular signal transduction

Inferred from electronic annotation. Source: Ensembl

lung development

Inferred from mutant phenotype PubMed 18213577. Source: MGI

negative regulation of cell death

Inferred from electronic annotation. Source: Ensembl

negative regulation of gene expression

Inferred from mutant phenotype PubMed 18597638. Source: MGI

organ senescence

Inferred from electronic annotation. Source: Ensembl

ossification

Inferred from mutant phenotype PubMed 11237711PubMed 12736220. Source: MGI

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of G0 to G1 transition

Inferred from electronic annotation. Source: Ensembl

positive regulation of JNK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle contraction

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell activation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell death

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of collagen biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of stress fiber assembly

Inferred from electronic annotation. Source: Ensembl

reactive oxygen species metabolic process

Inferred from direct assay PubMed 19364818. Source: MGI

regulation of cell growth

Inferred from electronic annotation. Source: InterPro

regulation of chondrocyte differentiation

Inferred from mutant phenotype PubMed 18597638. Source: MGI

response to amino acid

Inferred from electronic annotation. Source: Ensembl

response to anoxia

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to fatty acid

Inferred from electronic annotation. Source: Ensembl

response to glucose

Inferred from electronic annotation. Source: Ensembl

response to mineralocorticoid

Inferred from electronic annotation. Source: Ensembl

response to peptide hormone

Inferred from electronic annotation. Source: Ensembl

tissue homeostasis

Inferred from mutant phenotype PubMed 23839921. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

cell cortex

Inferred from electronic annotation. Source: Ensembl

cis-Golgi network

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

extracellular region

Inferred from direct assay Ref.1. Source: MGI

extracellular space

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

proteinaceous extracellular matrix

Inferred from direct assay Ref.6. Source: MGI

   Molecular_functionheparin binding

Inferred from direct assay Ref.6. Source: MGI

integrin binding

Inferred from direct assay PubMed 10446209. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 348323Connective tissue growth factor
PRO_0000014403

Regions

Domain26 – 9772IGFBP N-terminal
Domain100 – 16667VWFC
Domain197 – 24246TSP type-1
Domain255 – 32975CTCK
Region246 – 348103Heparin-binding By similarity

Amino acid modifications

Disulfide bond255 ↔ 292 By similarity
Disulfide bond272 ↔ 306 By similarity
Disulfide bond283 ↔ 322 By similarity
Disulfide bond286 ↔ 324 By similarity
Disulfide bond291 ↔ 328 By similarity

Experimental info

Sequence conflict241M → T in AAA37627. Ref.1
Sequence conflict241M → T in AAA37628. Ref.1
Sequence conflict241M → T in AAA73135. Ref.2
Sequence conflict241M → T in AAH06783. Ref.5
Sequence conflict1611E → K in AAA37627. Ref.1
Sequence conflict1611E → K in AAA37628. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P29268 [UniParc].

Last modified October 3, 2012. Version 3.
Checksum: 9BD99E6D0BEFC7BC

FASTA34837,824
        10         20         30         40         50         60 
MLASVAGPIS LALVLLALCT RPAMGQDCSA QCQCAAEAAP HCPAGVSLVL DGCGCCRVCA 

        70         80         90        100        110        120 
KQLGELCTER DPCDPHKGLF CDFGSPANRK IGVCTAKDGA PCVFGGSVYR SGESFQSSCK 

       130        140        150        160        170        180 
YQCTCLDGAV GCVPLCSMDV RLPSPDCPFP RRVKLPGKCC EEWVCDEPKD RTAVGPALAA 

       190        200        210        220        230        240 
YRLEDTFGPD PTMMRANCLV QTTEWSACSK TCGMGISTRV TNDNTFCRLE KQSRLCMVRP 

       250        260        270        280        290        300 
CEADLEENIK KGKKCIRTPK IAKPVKFELS GCTSVKTYRA KFCGVCTDGR CCTPHRTTTL 

       310        320        330        340 
PVEFKCPDGE IMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA 

« Hide

References

« Hide 'large scale' references
[1]"Structure, mapping, and expression of fisp-12, a growth factor-inducible gene encoding a secreted cysteine-rich protein."
Ryseck R.-P., Macdonald-Bravo H., Mattei M.-G., Bravo R.
Cell Growth Differ. 2:225-233(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"Identification of a gene family regulated by transforming growth factor-beta."
Brunner A., Chinn J., Neubauer M.G., Purchio A.F.
DNA Cell Biol. 10:293-300(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Cyr61 and Fisp12 are both ECM-associated signaling molecules: activities, metabolism, and localization during development."
Kireeva M.L., Latinkic B.V., Kolesnikova T.V., Chen C.-C., Yang G.P., Abler A.S., Lau L.F.
Exp. Cell Res. 233:63-77(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and migration through integrin alphavbeta3, promotes endothelial cell survival, and induces angiogenesis in vivo."
Babic A.M., Chen C.-C., Lau L.F.
Mol. Cell. Biol. 19:2958-2966(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M70641 Genomic DNA. Translation: AAA37627.1.
M70642 mRNA. Translation: AAA37628.1.
M80263 mRNA. Translation: AAA73135.1.
AC099695 Genomic DNA. No translation available.
CH466540 Genomic DNA. Translation: EDL04783.1.
BC006783 mRNA. Translation: AAH06783.1.
PIRA40578.
RefSeqNP_034347.2. NM_010217.2.
UniGeneMm.390287.

3D structure databases

ProteinModelPortalP29268.
SMRP29268. Positions 27-168, 200-241, 265-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000020171.

PTM databases

PhosphoSiteP29268.

Proteomic databases

PaxDbP29268.
PRIDEP29268.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020171; ENSMUSP00000020171; ENSMUSG00000019997.
GeneID14219.
KEGGmmu:14219.
UCSCuc011xbr.1. mouse.

Organism-specific databases

CTD1490.
MGIMGI:95537. Ctgf.

Phylogenomic databases

eggNOGNOG72934.
GeneTreeENSGT00750000117442.
HOGENOMHOG000231462.
HOVERGENHBG000635.
InParanoidP29268.
KOK06827.
OMAKTYRAKF.
OrthoDBEOG7N8ZXB.
TreeFamTF326070.

Gene expression databases

ArrayExpressP29268.
CleanExMM_CTGF.
GenevestigatorP29268.

Family and domain databases

InterProIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio285469.
PROP29268.
SOURCESearch...

Entry information

Entry nameCTGF_MOUSE
AccessionPrimary (citable) accession number: P29268
Secondary accession number(s): G5E830, Q922U0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot