Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P29268

- CTGF_MOUSE

UniProt

P29268 - CTGF_MOUSE

Protein

Connective tissue growth factor

Gene

Ctgf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 3 (03 Oct 2012)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes By similarity. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells By similarity. Enhances fibroblast growth factor-induced DNA synthesis By similarity.By similarity

    GO - Molecular functioni

    1. heparin binding Source: MGI
    2. integrin binding Source: MGI

    GO - Biological processi

    1. angiogenesis Source: MGI
    2. cartilage condensation Source: MGI
    3. cell differentiation Source: MGI
    4. cell-matrix adhesion Source: MGI
    5. cell migration Source: MGI
    6. chondrocyte proliferation Source: MGI
    7. connective tissue development Source: MGI
    8. cytosolic calcium ion transport Source: Ensembl
    9. DNA replication Source: UniProtKB-KW
    10. extracellular matrix constituent secretion Source: Ensembl
    11. fibroblast growth factor receptor signaling pathway Source: MGI
    12. integrin-mediated signaling pathway Source: MGI
    13. intracellular signal transduction Source: Ensembl
    14. lung development Source: MGI
    15. negative regulation of cell death Source: Ensembl
    16. negative regulation of gene expression Source: MGI
    17. organ senescence Source: Ensembl
    18. ossification Source: MGI
    19. positive regulation of cardiac muscle contraction Source: Ensembl
    20. positive regulation of cell activation Source: Ensembl
    21. positive regulation of cell death Source: Ensembl
    22. positive regulation of cell differentiation Source: Ensembl
    23. positive regulation of cell proliferation Source: Ensembl
    24. positive regulation of collagen biosynthetic process Source: Ensembl
    25. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    26. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
    27. positive regulation of G0 to G1 transition Source: Ensembl
    28. positive regulation of gene expression Source: Ensembl
    29. positive regulation of JNK cascade Source: Ensembl
    30. positive regulation of protein phosphorylation Source: Ensembl
    31. positive regulation of stress fiber assembly Source: Ensembl
    32. reactive oxygen species metabolic process Source: MGI
    33. regulation of cell growth Source: InterPro
    34. regulation of chondrocyte differentiation Source: MGI
    35. response to amino acid Source: Ensembl
    36. response to anoxia Source: Ensembl
    37. response to estradiol Source: Ensembl
    38. response to fatty acid Source: Ensembl
    39. response to glucose Source: Ensembl
    40. response to mineralocorticoid Source: Ensembl
    41. response to peptide hormone Source: Ensembl
    42. tissue homeostasis Source: MGI

    Keywords - Biological processi

    Cell adhesion, DNA synthesis

    Keywords - Ligandi

    Heparin-binding

    Enzyme and pathway databases

    ReactomeiREACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    REACT_198602. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Connective tissue growth factor
    Alternative name(s):
    CCN family member 2
    Hypertrophic chondrocyte-specific protein 24
    Protein FISP-12
    Gene namesi
    Name:Ctgf
    Synonyms:Ccn2, Fisp-12, Fisp12, Hcs24
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:95537. Ctgf.

    Subcellular locationi

    Secretedextracellular spaceextracellular matrix 1 Publication. Secreted 1 Publication

    GO - Cellular componenti

    1. cell cortex Source: Ensembl
    2. cis-Golgi network Source: Ensembl
    3. cytosol Source: Ensembl
    4. extracellular region Source: MGI
    5. extracellular space Source: Ensembl
    6. Golgi apparatus Source: Ensembl
    7. perinuclear region of cytoplasm Source: Ensembl
    8. proteinaceous extracellular matrix Source: MGI

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 348323Connective tissue growth factorPRO_0000014403Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi255 ↔ 292By similarity
    Disulfide bondi272 ↔ 306By similarity
    Disulfide bondi283 ↔ 322By similarity
    Disulfide bondi286 ↔ 324By similarity
    Disulfide bondi291 ↔ 328By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP29268.
    PRIDEiP29268.

    PTM databases

    PhosphoSiteiP29268.

    Expressioni

    Tissue specificityi

    Testis, spleen, kidney, lung, heart, and brain (lowest level in testis and highest in lung).

    Inductioni

    By growth factors.

    Gene expression databases

    ArrayExpressiP29268.
    CleanExiMM_CTGF.
    GenevestigatoriP29268.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000020171.

    Structurei

    3D structure databases

    ProteinModelPortaliP29268.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 9772IGFBP N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini100 – 16667VWFCPROSITE-ProRule annotationAdd
    BLAST
    Domaini197 – 24246TSP type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini255 – 32975CTCKPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni246 – 348103Heparin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CCN family.Curated
    Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
    Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
    Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
    Contains 1 VWFC domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG72934.
    GeneTreeiENSGT00750000117442.
    HOGENOMiHOG000231462.
    HOVERGENiHBG000635.
    InParanoidiP29268.
    KOiK06827.
    OMAiKTYRAKF.
    OrthoDBiEOG7N8ZXB.
    TreeFamiTF326070.

    Family and domain databases

    InterProiIPR006207. Cys_knot_C.
    IPR006208. Glyco_hormone_CN.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000867. IGFBP-like.
    IPR012395. IGFBP_CNN.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000884. Thrombospondin_1_rpt.
    IPR001007. VWF_C.
    [Graphical view]
    PfamiPF00007. Cys_knot. 1 hit.
    PF00219. IGFBP. 1 hit.
    PF00090. TSP_1. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
    SMARTiSM00041. CT. 1 hit.
    SM00121. IB. 1 hit.
    SM00209. TSP1. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view]
    SUPFAMiSSF57184. SSF57184. 1 hit.
    SSF82895. SSF82895. 1 hit.
    PROSITEiPS01185. CTCK_1. 1 hit.
    PS01225. CTCK_2. 1 hit.
    PS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS50092. TSP1. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29268-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLASVAGPIS LALVLLALCT RPAMGQDCSA QCQCAAEAAP HCPAGVSLVL    50
    DGCGCCRVCA KQLGELCTER DPCDPHKGLF CDFGSPANRK IGVCTAKDGA 100
    PCVFGGSVYR SGESFQSSCK YQCTCLDGAV GCVPLCSMDV RLPSPDCPFP 150
    RRVKLPGKCC EEWVCDEPKD RTAVGPALAA YRLEDTFGPD PTMMRANCLV 200
    QTTEWSACSK TCGMGISTRV TNDNTFCRLE KQSRLCMVRP CEADLEENIK 250
    KGKKCIRTPK IAKPVKFELS GCTSVKTYRA KFCGVCTDGR CCTPHRTTTL 300
    PVEFKCPDGE IMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA 348
    Length:348
    Mass (Da):37,824
    Last modified:October 3, 2012 - v3
    Checksum:i9BD99E6D0BEFC7BC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241M → T in AAA37627. (PubMed:1888698)Curated
    Sequence conflicti24 – 241M → T in AAA37628. (PubMed:1888698)Curated
    Sequence conflicti24 – 241M → T in AAA73135. (PubMed:2029337)Curated
    Sequence conflicti24 – 241M → T in AAH06783. (PubMed:15489334)Curated
    Sequence conflicti161 – 1611E → K in AAA37627. (PubMed:1888698)Curated
    Sequence conflicti161 – 1611E → K in AAA37628. (PubMed:1888698)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M70641 Genomic DNA. Translation: AAA37627.1.
    M70642 mRNA. Translation: AAA37628.1.
    M80263 mRNA. Translation: AAA73135.1.
    AC099695 Genomic DNA. No translation available.
    CH466540 Genomic DNA. Translation: EDL04783.1.
    BC006783 mRNA. Translation: AAH06783.1.
    CCDSiCCDS23751.1.
    PIRiA40578.
    RefSeqiNP_034347.2. NM_010217.2.
    UniGeneiMm.390287.

    Genome annotation databases

    EnsembliENSMUST00000020171; ENSMUSP00000020171; ENSMUSG00000019997.
    GeneIDi14219.
    KEGGimmu:14219.
    UCSCiuc011xbr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M70641 Genomic DNA. Translation: AAA37627.1 .
    M70642 mRNA. Translation: AAA37628.1 .
    M80263 mRNA. Translation: AAA73135.1 .
    AC099695 Genomic DNA. No translation available.
    CH466540 Genomic DNA. Translation: EDL04783.1 .
    BC006783 mRNA. Translation: AAH06783.1 .
    CCDSi CCDS23751.1.
    PIRi A40578.
    RefSeqi NP_034347.2. NM_010217.2.
    UniGenei Mm.390287.

    3D structure databases

    ProteinModelPortali P29268.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000020171.

    PTM databases

    PhosphoSitei P29268.

    Proteomic databases

    PaxDbi P29268.
    PRIDEi P29268.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020171 ; ENSMUSP00000020171 ; ENSMUSG00000019997 .
    GeneIDi 14219.
    KEGGi mmu:14219.
    UCSCi uc011xbr.1. mouse.

    Organism-specific databases

    CTDi 1490.
    MGIi MGI:95537. Ctgf.

    Phylogenomic databases

    eggNOGi NOG72934.
    GeneTreei ENSGT00750000117442.
    HOGENOMi HOG000231462.
    HOVERGENi HBG000635.
    InParanoidi P29268.
    KOi K06827.
    OMAi KTYRAKF.
    OrthoDBi EOG7N8ZXB.
    TreeFami TF326070.

    Enzyme and pathway databases

    Reactomei REACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    REACT_198602. PPARA activates gene expression.

    Miscellaneous databases

    NextBioi 285469.
    PROi P29268.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P29268.
    CleanExi MM_CTGF.
    Genevestigatori P29268.

    Family and domain databases

    InterProi IPR006207. Cys_knot_C.
    IPR006208. Glyco_hormone_CN.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR000867. IGFBP-like.
    IPR012395. IGFBP_CNN.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000884. Thrombospondin_1_rpt.
    IPR001007. VWF_C.
    [Graphical view ]
    Pfami PF00007. Cys_knot. 1 hit.
    PF00219. IGFBP. 1 hit.
    PF00090. TSP_1. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036495. IGFBP_rP_CNN. 1 hit.
    SMARTi SM00041. CT. 1 hit.
    SM00121. IB. 1 hit.
    SM00209. TSP1. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57184. SSF57184. 1 hit.
    SSF82895. SSF82895. 1 hit.
    PROSITEi PS01185. CTCK_1. 1 hit.
    PS01225. CTCK_2. 1 hit.
    PS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS50092. TSP1. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure, mapping, and expression of fisp-12, a growth factor-inducible gene encoding a secreted cysteine-rich protein."
      Ryseck R.-P., Macdonald-Bravo H., Mattei M.-G., Bravo R.
      Cell Growth Differ. 2:225-233(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. "Identification of a gene family regulated by transforming growth factor-beta."
      Brunner A., Chinn J., Neubauer M.G., Purchio A.F.
      DNA Cell Biol. 10:293-300(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Cyr61 and Fisp12 are both ECM-associated signaling molecules: activities, metabolism, and localization during development."
      Kireeva M.L., Latinkic B.V., Kolesnikova T.V., Chen C.-C., Yang G.P., Abler A.S., Lau L.F.
      Exp. Cell Res. 233:63-77(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and migration through integrin alphavbeta3, promotes endothelial cell survival, and induces angiogenesis in vivo."
      Babic A.M., Chen C.-C., Lau L.F.
      Mol. Cell. Biol. 19:2958-2966(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCTGF_MOUSE
    AccessioniPrimary (citable) accession number: P29268
    Secondary accession number(s): G5E830, Q922U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3