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P29241

- NADA_APLCA

UniProt

P29241 - NADA_APLCA

Protein

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase

Gene
N/A
Organism
Aplysia californica (California sea hare)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for calcium mobilization from endoplasmic reticulum. Also has cADPr hydrolase activity.1 Publication

    Catalytic activityi

    NAD+ + H2O = ADP-D-ribose + nicotinamide.1 Publication
    NADP+ + nicotinate = nicotinate-adenine dinucleotide phosphate + nicotinamide.1 Publication

    Enzyme regulationi

    Activity is presumably regulated by its sequestration in vesicles before egg fertilization. After fertilization and upon NADase release, it could then be regulated via its potential phosphorylation sites.

    GO - Molecular functioni

    1. NAD(P)+ nucleosidase activity Source: UniProtKB-EC
    2. NAD+ nucleosidase activity Source: InterPro
    3. transferase activity Source: UniProtKB-KW

    GO - Biological processi

    1. single fertilization Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transferase

    Keywords - Biological processi

    Fertilization

    Keywords - Ligandi

    Calcium, NAD, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (EC:3.2.2.6)
    Alternative name(s):
    2'-phospho-ADP-ribosyl cyclase
    2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20)
    2'-phospho-cyclic-ADP-ribose transferase
    ADP-ribosyl cyclase
    Short name:
    ADPRC
    Short name:
    ADRC
    NAD glycohydrolase
    NAD(+) nucleosidase
    Short name:
    NADase
    OrganismiAplysia californica (California sea hare)
    Taxonomic identifieri6500 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

    Subcellular locationi

    Cytoplasmic vesicle
    Note: Localized to vesicles or granules within ova of all stages.

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24242 PublicationsAdd
    BLAST
    Chaini25 – 282258ADP-ribosyl cyclase/cyclic ADP-ribose hydrolasePRO_0000004030Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi39 ↔ 58
    Disulfide bondi75 ↔ 155
    Disulfide bondi136 ↔ 149
    Disulfide bondi230 ↔ 251
    Disulfide bondi263 ↔ 272

    Post-translational modificationi

    Has different isoforms which may be the result of different amounts of phosphorylation.

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Expressioni

    Tissue specificityi

    Oocytes.

    Developmental stagei

    Immature eggs have higher levels of NADase transcripts than the mature ones.

    Structurei

    Secondary structure

    1
    282
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi31 – 4515
    Turni48 – 503
    Helixi58 – 6912
    Helixi74 – 763
    Turni79 – 824
    Helixi83 – 897
    Turni95 – 973
    Beta strandi98 – 1036
    Helixi105 – 1128
    Turni113 – 1164
    Helixi121 – 1233
    Helixi125 – 1306
    Helixi152 – 1543
    Helixi157 – 1604
    Helixi162 – 17413
    Beta strandi177 – 18610
    Beta strandi188 – 1903
    Beta strandi195 – 1973
    Helixi198 – 2014
    Helixi204 – 2063
    Beta strandi211 – 2199
    Beta strandi222 – 2243
    Beta strandi230 – 2323
    Helixi233 – 24412
    Beta strandi248 – 2547
    Helixi256 – 2649
    Helixi270 – 2723

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LBEX-ray2.40A/B25-282[»]
    1R0SX-ray2.00A/B25-282[»]
    1R12X-ray1.70A/B25-282[»]
    1R15X-ray2.40A/B/C/D/E/F/G/H25-282[»]
    1R16X-ray2.00A/B25-282[»]
    3I9JX-ray2.18A/B25-282[»]
    3I9KX-ray1.83A/B25-282[»]
    3I9LX-ray1.75A/B25-282[»]
    3I9OX-ray3.00A/B25-282[»]
    3ZWMX-ray2.50A/B/C/D/E/F/G/H25-282[»]
    3ZWNX-ray1.80A/B25-282[»]
    3ZWOX-ray2.00A/B/C/D/E/F/G/H24-282[»]
    3ZWPX-ray2.11A/B/C/D/E/F/G/H25-282[»]
    3ZWVX-ray2.30A/B/C/D/E/F/G/H25-282[»]
    3ZWWX-ray2.30A/B/C/D/E/F/G/H25-282[»]
    3ZWXX-ray2.60A/B/C/D/E/F/G/H25-282[»]
    3ZWYX-ray2.40A/B/C/D/E/F/G/H25-282[»]
    ProteinModelPortaliP29241.
    SMRiP29241. Positions 25-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29241.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ADP-ribosyl cyclase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR003193. ADP-ribosyl_cyclase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10912. PTHR10912. 1 hit.
    PfamiPF02267. Rib_hydrolayse. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29241-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPVAIIACV CLAVTLTSIS PSEAIVPTRE LENVFLGRCK DYEITRYLDI    50
    LPRVRSDCSA LWKDFFKAFS FKNPCDLDLG SYKDFFTSAQ QQLPKNKVMF 100
    WSGVYDEAHD YANTGRKYIT LEDTLPGYML NSLVWCGQRA NPGFNEKVCP 150
    DFKTCPVQAR ESFWGMASSS YAHSAEGEVT YMVDGSNPKV PAYRPDSFFG 200
    KYELPNLTNK VTRVKVIVLH RLGEKIIEKC GAGSLLDLEK LVKAKHFAFD 250
    CVENPRAVLF LLCSDNPNAR ECRLAKRFYR IA 282
    Length:282
    Mass (Da):31,899
    Last modified:December 1, 1992 - v1
    Checksum:i4CF809DF4E30A824
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85206 mRNA. Translation: AAA65698.1.
    PIRiS27769.
    RefSeqiNP_001191476.1. NM_001204547.1.
    UniGeneiAcl.44579.

    Genome annotation databases

    GeneIDi100533234.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85206 mRNA. Translation: AAA65698.1 .
    PIRi S27769.
    RefSeqi NP_001191476.1. NM_001204547.1.
    UniGenei Acl.44579.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LBE X-ray 2.40 A/B 25-282 [» ]
    1R0S X-ray 2.00 A/B 25-282 [» ]
    1R12 X-ray 1.70 A/B 25-282 [» ]
    1R15 X-ray 2.40 A/B/C/D/E/F/G/H 25-282 [» ]
    1R16 X-ray 2.00 A/B 25-282 [» ]
    3I9J X-ray 2.18 A/B 25-282 [» ]
    3I9K X-ray 1.83 A/B 25-282 [» ]
    3I9L X-ray 1.75 A/B 25-282 [» ]
    3I9O X-ray 3.00 A/B 25-282 [» ]
    3ZWM X-ray 2.50 A/B/C/D/E/F/G/H 25-282 [» ]
    3ZWN X-ray 1.80 A/B 25-282 [» ]
    3ZWO X-ray 2.00 A/B/C/D/E/F/G/H 24-282 [» ]
    3ZWP X-ray 2.11 A/B/C/D/E/F/G/H 25-282 [» ]
    3ZWV X-ray 2.30 A/B/C/D/E/F/G/H 25-282 [» ]
    3ZWW X-ray 2.30 A/B/C/D/E/F/G/H 25-282 [» ]
    3ZWX X-ray 2.60 A/B/C/D/E/F/G/H 25-282 [» ]
    3ZWY X-ray 2.40 A/B/C/D/E/F/G/H 25-282 [» ]
    ProteinModelPortali P29241.
    SMRi P29241. Positions 25-275.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100533234.

    Organism-specific databases

    CTDi 100533234.

    Miscellaneous databases

    EvolutionaryTracei P29241.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR003193. ADP-ribosyl_cyclase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10912. PTHR10912. 1 hit.
    Pfami PF02267. Rib_hydrolayse. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of a molluscan egg-specific NADase, a second-messenger enzyme."
      Glick D.L., Hellmich M.R., Beushausen S., Tempst P.J., Bayley H., Strumwasser F.
      Cell Regul. 2:211-218(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-72; 84-95; 98-114; 190-209 AND 216-225.
      Tissue: Ovotestis.
    2. "Unifying mechanism for Aplysia ADP-ribosyl cyclase and CD38/NAD(+) glycohydrolases."
      Cakir-Kiefer C., Muller-Steffner H., Schuber F.
      Biochem. J. 349:203-210(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-42, CATALYTIC ACTIVITY.
    3. "Purification and characterization of a molluscan egg-specific NADase, a second-messenger enzyme."
      Hellmich M.R., Strumwasser F.
      Cell Regul. 2:193-202(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Similarities in amino acid sequences of Aplysia ADP-ribosyl cyclase and human lymphocyte antigen CD38."
      States D.J., Walseth T.F., Lee H.C.
      Trends Biochem. Sci. 17:495-495(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO CD38.
    5. "CD38 is the major enzyme responsible for synthesis of nicotinic acid-adenine dinucleotide phosphate in mammalian tissues."
      Chini E.N., Chini C.C., Kato I., Takasawa S., Okamoto H.
      Biochem. J. 362:125-130(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN SYNTHESIS OF NICOTINIC ACID-ADENINE DINUCLEOTIDE PHOSPHATE.
    6. "Crystal structure of Aplysia ADP ribosyl cyclase, a homologue of the bifunctional ectozyme CD38."
      Prasad G.S., McRee D.E., Stura E.A., Levitt D.G., Lee H.C., Stout C.D.
      Nat. Struct. Biol. 3:957-964(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

    Entry informationi

    Entry nameiNADA_APLCA
    AccessioniPrimary (citable) accession number: P29241
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3