Reviewed, UniProtKB/Swiss-Prot P29239 (LUXB2_PHOLE)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase beta chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase beta chain

Gene names

Name:

luxB

Organism

Photobacterium leiognathi

Taxonomic identifier

553611 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Alkanal monooxygenase beta chain		PRO_0000220175

Sequences

	Sequence		Length	Mass (Da)
	P29239-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: E7E5C05069B64688	FASTA	326	37,701
	10 20 30 40 50 60 MNFGLFFLNF QPEGMTSEMV LDNMVDTVAL VDKDDYHFKR VLVSEHHFSK NGIIGEPLTA 70 80 90 100 110 120 ISFLLGLTKR IEIGSLNQVI TTHHPVRIGE QTGLLDQMSY GRFVLGLSDC VNDFEMDFFK 130 140 150 160 170 180 RKRSSQQQQF EACYEILNEA LTTNYCQADD DFFNFPRISV NPHCISEVKQ YILASSMGVV 190 200 210 220 230 240 EWAARKGLPL TYRWSDSLAE KEKYYQRYLA VAKENNIDVS NIDHQFPLLV NINENRRIAR 250 260 270 280 290 300 DEVREYIQSY VSEAYPTDPN IELRVEELIE QHAVGKVDEY YDSTMHAVKV TGSKNLLLSF 310 320 ESMKNKDDVT KLINMFNQKI KDNLIK

References

[1]

"The lux genes of the luminous bacterial symbiont, Photobacterium leiognathi, of the ponyfish. Nucleotide sequence, difference in gene organization, and high expression in mutant Escherichia coli." Lee C.Y., Szittner R.B., Meighen E.A. Eur. J. Biochem. 201:161-167(1991) [PubMed: 1915359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25521 / L1 / CIP 665.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63594 Genomic DNA. Translation: AAA25619.1.
PIR	S17954.
3D structure databases
SMR	P29239. Positions 1-320, 97-323.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.3. 349.
Family and domain databases
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like. IPR002103. Luciferase_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXB2_PHOLE

Accession

Primary (citable) accession number: P29239

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)