Reviewed, UniProtKB/Swiss-Prot P29219 (IMPA1_XENLA)

Names and origin

Protein names

Recommended name:
    Inositol monophosphatase
    EC=3.1.3.25
Alternative name(s):
    Inositol-1(or 4)-monophosphatase
      Short name=IMPase
      Short name=IMP

Gene names

Name:

impa1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides.
Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inhibited by Li+ By similarity.
Pathway	Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inositol-1(or 4)-monophosphatase activity Inferred from electronic annotation. Source: EC lithium ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Inositol monophosphatase		PRO_0000142518
Sites
	Metal binding	73	1	Magnesium 1 By similarity
	Metal binding	93	1	Magnesium 1 By similarity
	Metal binding	93	1	Magnesium 2 By similarity
	Metal binding	95	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	96	1	Magnesium 2 By similarity
	Metal binding	224	1	Magnesium 2 By similarity
	Binding site	96	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29219-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 34E9CD9A99231292	FASTA	285	30,729
	10 20 30 40 50 60 MEDRWQECMD FLAVSIARKA GSVVCAALKE DVSIMVKTSL APADLVTATD QKVEEMIISS 70 80 90 100 110 120 IKEKYPSHSF IGEESVAAGA GSTLTDNPTW IIDPIDGTTN FVHRFPFVAV SIGFAVHKQV 130 140 150 160 170 180 EFGVVYSCVE DKMYTGRKGK GSFCNGQKLQ VSGQKDITKS MIITELGSNR NPEFIKTVSL 190 200 210 220 230 240 SNMERLLCIP IHGIRAVGTA AVNMCLVATG GADAYYEMGL HCWDMAAASV IVTEAGGTIL 250 260 270 280 DATGGLFDLM SCRIISASSR EIAERIAKEL QIIPLERDDG KSTNS

References

[1]	"Inositol monophosphatase is a highly conserved enzyme having localized structural similarity to both glycerol 3-phosphate dehydrogenase and haemoglobin." Wreggett K.A. Biochem. J. 286:147-152(1992) [PubMed: 1325777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Oocyte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65513 mRNA. Translation: CAA46486.1.
PIR	S24343.
RefSeq	NP_001095235.1.
UniGene	Xl.3663
3D structure databases
HSSP	HSSP built from PDB template 1IMF based on UniProtKB P29218.
SMR	P29219. Positions 5-279.
ModBase	Search...
Genome annotation databases
GeneID	397905.
KEGG	xla:397905.
Phylogenomic databases
HOVERGEN	P29219.
Enzyme and pathway databases
BRENDA	3.1.3.25. 648.
Family and domain databases
InterPro	IPR000760. Inositol_P. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00378. INOSPHPHTASE.
ProDom	PD023420. Inositol_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMPA1_XENLA

Accession

Primary (citable) accession number: P29219

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project