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P29208

- MENC_ECOLI

UniProt

P29208 - MENC_ECOLI

Protein

o-succinylbenzoate synthase

Gene

menC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Converts SHCHC to OSB.

    Catalytic activityi

    (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O.

    Cofactori

    Magnesium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei133 – 1331Proton donor
    Metal bindingi161 – 1611Magnesium
    Metal bindingi190 – 1901Magnesium
    Metal bindingi213 – 2131Magnesium
    Active sitei235 – 2351Proton acceptor

    GO - Molecular functioni

    1. hydro-lyase activity Source: EcoCyc
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. menaquinone biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Menaquinone biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:O-SUCCINYLBENZOATE-COA-SYN-MONOMER.
    ECOL316407:JW2256-MONOMER.
    MetaCyc:O-SUCCINYLBENZOATE-COA-SYN-MONOMER.
    BRENDAi4.2.1.113. 2026.
    SABIO-RKP29208.
    UniPathwayiUPA00079; UER00165.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    o-succinylbenzoate synthase (EC:4.2.1.113)
    Short name:
    OSB synthase
    Short name:
    OSBS
    Alternative name(s):
    4-(2'-carboxyphenyl)-4-oxybutyric acid synthase
    o-succinylbenzoic acid synthase
    Gene namesi
    Name:menC
    Ordered Locus Names:b2261, JW2256
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11532. menC.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320o-succinylbenzoate synthasePRO_0000171270Add
    BLAST

    Proteomic databases

    PaxDbiP29208.

    Expressioni

    Gene expression databases

    GenevestigatoriP29208.

    Interactioni

    Protein-protein interaction databases

    IntActiP29208. 5 interactions.
    MINTiMINT-1305669.
    STRINGi511145.b2261.

    Structurei

    Secondary structure

    1
    320
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1312
    Beta strandi26 – 3611
    Beta strandi39 – 457
    Turni49 – 513
    Beta strandi52 – 543
    Helixi56 – 7015
    Helixi81 – 9414
    Beta strandi109 – 1113
    Helixi114 – 1229
    Beta strandi126 – 1338
    Beta strandi135 – 1373
    Helixi139 – 15214
    Beta strandi156 – 1616
    Helixi168 – 1769
    Turni180 – 1823
    Helixi183 – 1853
    Beta strandi186 – 1905
    Beta strandi193 – 1953
    Helixi196 – 20611
    Beta strandi210 – 2134
    Helixi214 – 2174
    Beta strandi229 – 2346
    Helixi236 – 2394
    Helixi242 – 25413
    Beta strandi258 – 2625
    Helixi268 – 28114
    Helixi292 – 2943
    Beta strandi298 – 3014
    Helixi313 – 3153
    Beta strandi316 – 3183

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FHUX-ray1.65A1-320[»]
    1FHVX-ray1.77A1-320[»]
    1R6WX-ray1.62A1-320[»]
    2OFJX-ray2.30A/B/C/D1-320[»]
    ProteinModelPortaliP29208.
    SMRiP29208. Positions 1-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29208.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1441.
    HOGENOMiHOG000271247.
    KOiK02549.
    OMAiYEANRDG.
    OrthoDBiEOG6VXFBR.
    PhylomeDBiP29208.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_00470. MenC_1.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR013342. Mandelate_racemase_C.
    IPR001354. MR_MLE.
    IPR010196. OSB_synthase.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF01188. MR_MLE. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01927. menC_gamma/gm+. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P29208-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSAQVYRWQ IPMDAGVVLR DRRLKTRDGL YVCLREGERE GWGEISPLPG    50
    FSQETWEEAQ SVLLAWVNNW LAGDCELPQM PSVAFGVSCA LAELTDTLPQ 100
    AANYRAAPLC NGDPDDLILK LADMPGEKVA KVKVGLYEAV RDGMVVNLLL 150
    EAIPDLHLRL DANRAWTPLK GQQFAKYVNP DYRDRIAFLE EPCKTRDDSR 200
    AFARETGIAI AWDESLREPD FAFVAEEGVR AVVIKPTLTG SLEKVREQVQ 250
    AAHALGLTAV ISSSIESSLG LTQLARIAAW LTPDTIPGLD TLDLMQAQQV 300
    RRWPGSTLPV VEVDALERLL 320
    Length:320
    Mass (Da):35,477
    Last modified:November 1, 1997 - v2
    Checksum:iE20245EC13D06839
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti176 – 1772Missing in AAA71917. (PubMed:8335646)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07256 Unassigned DNA. Translation: AAA71917.1.
    U00096 Genomic DNA. Translation: AAC75321.1.
    AP009048 Genomic DNA. Translation: BAA16085.2.
    PIRiC64997.
    RefSeqiNP_416764.1. NC_000913.3.
    YP_490501.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75321; AAC75321; b2261.
    BAA16085; BAA16085; BAA16085.
    GeneIDi12931509.
    946734.
    KEGGiecj:Y75_p2225.
    eco:b2261.
    PATRICi32119889. VBIEscCol129921_2354.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07256 Unassigned DNA. Translation: AAA71917.1 .
    U00096 Genomic DNA. Translation: AAC75321.1 .
    AP009048 Genomic DNA. Translation: BAA16085.2 .
    PIRi C64997.
    RefSeqi NP_416764.1. NC_000913.3.
    YP_490501.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FHU X-ray 1.65 A 1-320 [» ]
    1FHV X-ray 1.77 A 1-320 [» ]
    1R6W X-ray 1.62 A 1-320 [» ]
    2OFJ X-ray 2.30 A/B/C/D 1-320 [» ]
    ProteinModelPortali P29208.
    SMRi P29208. Positions 1-320.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P29208. 5 interactions.
    MINTi MINT-1305669.
    STRINGi 511145.b2261.

    Proteomic databases

    PaxDbi P29208.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75321 ; AAC75321 ; b2261 .
    BAA16085 ; BAA16085 ; BAA16085 .
    GeneIDi 12931509.
    946734.
    KEGGi ecj:Y75_p2225.
    eco:b2261.
    PATRICi 32119889. VBIEscCol129921_2354.

    Organism-specific databases

    EchoBASEi EB1494.
    EcoGenei EG11532. menC.

    Phylogenomic databases

    eggNOGi COG1441.
    HOGENOMi HOG000271247.
    KOi K02549.
    OMAi YEANRDG.
    OrthoDBi EOG6VXFBR.
    PhylomeDBi P29208.

    Enzyme and pathway databases

    UniPathwayi UPA00079 ; UER00165 .
    BioCyci EcoCyc:O-SUCCINYLBENZOATE-COA-SYN-MONOMER.
    ECOL316407:JW2256-MONOMER.
    MetaCyc:O-SUCCINYLBENZOATE-COA-SYN-MONOMER.
    BRENDAi 4.2.1.113. 2026.
    SABIO-RK P29208.

    Miscellaneous databases

    EvolutionaryTracei P29208.
    PROi P29208.

    Gene expression databases

    Genevestigatori P29208.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPi MF_00470. MenC_1.
    InterProi IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR013342. Mandelate_racemase_C.
    IPR001354. MR_MLE.
    IPR010196. OSB_synthase.
    [Graphical view ]
    PANTHERi PTHR13794. PTHR13794. 1 hit.
    Pfami PF01188. MR_MLE. 1 hit.
    [Graphical view ]
    SMARTi SM00922. MR_MLE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR01927. menC_gamma/gm+. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Menaquinone (vitamin K2) biosynthesis: cloning, nucleotide sequence, and expression of the menC gene from Escherichia coli."
      Sharma V., Meganathan R., Hudspeth M.E.S.
      J. Bacteriol. 175:4917-4921(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Unexpected divergence of enzyme function and sequence: 'N-acylamino acid racemase' is o-succinylbenzoate synthase."
      Palmer D.R., Garrett J.B., Sharma V., Meganathan R., Babbitt P.C., Gerlt J.A.
      Biochemistry 38:4252-4258(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg(2+) and o-Succinylbenzoate."
      Thompson T.B., Garrett J.B., Taylor E.A., Meganathan R., Gerlt J.A., Rayment I.
      Biochemistry 39:10662-10676(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

    Entry informationi

    Entry nameiMENC_ECOLI
    AccessioniPrimary (citable) accession number: P29208
    Secondary accession number(s): P76476, P76931
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3