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Protein

Chaperonin CPN60, mitochondrial

Gene

CPN60

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

GO - Molecular functioni

  • ATP binding Source: TAIR
  • copper ion binding Source: TAIR
  • protein binding involved in protein folding Source: GO_Central
  • structural constituent of ribosome Source: CAFA
  • unfolded protein binding Source: GO_Central

GO - Biological processi

  • 'de novo' protein folding Source: GO_Central
  • chaperone-mediated protein folding Source: GO_Central
  • mitochondrion organization Source: TAIR
  • protein import into mitochondrial intermembrane space Source: GO_Central
  • protein refolding Source: InterPro
  • response to cadmium ion Source: TAIR
  • response to heat Source: TAIR
  • ribosome assembly Source: CAFA

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperonin CPN60, mitochondrial
Alternative name(s):
HSP60
Gene namesi
Name:CPN60
Ordered Locus Names:At3g23990
ORF Names:F14O13.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G23990.
TAIRilocus:2076081. AT3G23990.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31Mitochondrion1 PublicationAdd BLAST31
ChainiPRO_000000500932 – 577Chaperonin CPN60, mitochondrialAdd BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP29197.
PRIDEiP29197.

PTM databases

iPTMnetiP29197.

Expressioni

Inductioni

By heat shock.

Gene expression databases

ExpressionAtlasiP29197. baseline and differential.
GenevisibleiP29197. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi7315. 5 interactors.
MINTiMINT-8065192.
STRINGi3702.AT3G23990.1.

Structurei

3D structure databases

ProteinModelPortaliP29197.
SMRiP29197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0356. Eukaryota.
COG0459. LUCA.
HOGENOMiHOG000076290.
InParanoidiP29197.
KOiK04077.
OMAiTDTDKME.
OrthoDBiEOG093606CJ.
PhylomeDBiP29197.

Family and domain databases

CDDicd03344. GroEL. 1 hit.
Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60. 1 hit.
InterProiView protein in InterPro
IPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
PfamiView protein in Pfam
PF00118. Cpn60_TCP1. 1 hit.
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiView protein in PROSITE
PS00296. CHAPERONINS_CPN60. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRFASNLAS KARIAQNARQ VSSRMSWSRN YAAKEIKFGV EARALMLKGV
60 70 80 90 100
EDLADAVKVT MGPKGRNVVI EQSWGAPKVT KDGVTVAKSI EFKDKIKNVG
110 120 130 140 150
ASLVKQVANA TNDVAGDGTT CATVLTRAIF AEGCKSVAAG MNAMDLRRGI
160 170 180 190 200
SMAVDAVVTN LKSKARMIST SEEIAQVGTI SANGEREIGE LIAKAMEKVG
210 220 230 240 250
KEGVITIQDG KTLFNELEVV EGMKLDRGYT SPYFITNQKT QKCELDDPLI
260 270 280 290 300
LIHEKKISSI NSIVKVLELA LKRQRPLLIV SEDVESDALA TLILNKLRAG
310 320 330 340 350
IKVCAIKAPG FGENRKANLQ DLAALTGGEV ITDELGMNLE KVDLSMLGTC
360 370 380 390 400
KKVTVSKDDT VILDGAGDKK GIEERCEQIR SAIELSTSDY DKEKLQERLA
410 420 430 440 450
KLSGGVAVLK IGGASEAEVG EKKDRVTDAL NATKAAVEEG ILPGGGVALL
460 470 480 490 500
YAARELEKLP TANFDQKIGV QIIQNALKTP VYTIASNAGV EGAVIVGKLL
510 520 530 540 550
EQDNPDLGYD AAKGEYVDMV KAGIIDPLKV IRTALVDAAS VSSLLTTTEA
560 570
VVVDLPKDES ESGAAGAGMG GMGGMDY
Length:577
Mass (Da):61,281
Last modified:December 1, 2000 - v2
Checksum:i829ABEE93BFBB5DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti567A → G in CAA77646 (PubMed:1349837).Curated1
Sequence conflicti573 – 574GG → VV in CAA77646 (PubMed:1349837).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11547 mRNA. Translation: CAA77646.1.
AP001297 Genomic DNA. Translation: BAB03017.1.
CP002686 Genomic DNA. Translation: AEE76842.1.
AY099594 mRNA. Translation: AAM20445.1.
BT010398 mRNA. Translation: AAQ56841.1.
PIRiS20876.
RefSeqiNP_189041.1. NM_113304.5.
UniGeneiAt.25152.

Genome annotation databases

EnsemblPlantsiAT3G23990.1; AT3G23990.1; AT3G23990.
GeneIDi821983.
GrameneiAT3G23990.1; AT3G23990.1; AT3G23990.
KEGGiath:AT3G23990.

Similar proteinsi

Entry informationi

Entry nameiCH60A_ARATH
AccessioniPrimary (citable) accession number: P29197
Secondary accession number(s): Q9LIQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 2000
Last modified: September 27, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families