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Protein

Chaperonin CPN60, mitochondrial

Gene

CPN60

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

GO - Molecular functioni

  • ATP binding Source: TAIR
  • copper ion binding Source: TAIR

GO - Biological processi

  • mitochondrion organization Source: TAIR
  • protein refolding Source: InterPro
  • response to cadmium ion Source: TAIR
  • response to heat Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperonin CPN60, mitochondrial
Alternative name(s):
HSP60
Gene namesi
Name:CPN60
Ordered Locus Names:At3g23990
ORF Names:F14O13.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G23990.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • mitochondrial matrix Source: TAIR
  • mitochondrion Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131MitochondrionSequence analysisAdd
BLAST
Chaini32 – 577546Chaperonin CPN60, mitochondrialPRO_0000005009Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP29197.
PRIDEiP29197.

PTM databases

iPTMnetiP29197.

Expressioni

Inductioni

By heat shock.

Gene expression databases

GenevisibleiP29197. AT.

Interactioni

Protein-protein interaction databases

BioGridi7315. 5 interactions.
MINTiMINT-8065192.
STRINGi3702.AT3G23990.1.

Structurei

3D structure databases

ProteinModelPortaliP29197.
SMRiP29197. Positions 32-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0356. Eukaryota.
COG0459. LUCA.
HOGENOMiHOG000076290.
InParanoidiP29197.
KOiK04077.
OMAiSNAMKKV.
PhylomeDBiP29197.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60.
InterProiIPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiPS00296. CHAPERONINS_CPN60. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRFASNLAS KARIAQNARQ VSSRMSWSRN YAAKEIKFGV EARALMLKGV
60 70 80 90 100
EDLADAVKVT MGPKGRNVVI EQSWGAPKVT KDGVTVAKSI EFKDKIKNVG
110 120 130 140 150
ASLVKQVANA TNDVAGDGTT CATVLTRAIF AEGCKSVAAG MNAMDLRRGI
160 170 180 190 200
SMAVDAVVTN LKSKARMIST SEEIAQVGTI SANGEREIGE LIAKAMEKVG
210 220 230 240 250
KEGVITIQDG KTLFNELEVV EGMKLDRGYT SPYFITNQKT QKCELDDPLI
260 270 280 290 300
LIHEKKISSI NSIVKVLELA LKRQRPLLIV SEDVESDALA TLILNKLRAG
310 320 330 340 350
IKVCAIKAPG FGENRKANLQ DLAALTGGEV ITDELGMNLE KVDLSMLGTC
360 370 380 390 400
KKVTVSKDDT VILDGAGDKK GIEERCEQIR SAIELSTSDY DKEKLQERLA
410 420 430 440 450
KLSGGVAVLK IGGASEAEVG EKKDRVTDAL NATKAAVEEG ILPGGGVALL
460 470 480 490 500
YAARELEKLP TANFDQKIGV QIIQNALKTP VYTIASNAGV EGAVIVGKLL
510 520 530 540 550
EQDNPDLGYD AAKGEYVDMV KAGIIDPLKV IRTALVDAAS VSSLLTTTEA
560 570
VVVDLPKDES ESGAAGAGMG GMGGMDY
Length:577
Mass (Da):61,281
Last modified:December 1, 2000 - v2
Checksum:i829ABEE93BFBB5DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti567 – 5671A → G in CAA77646 (PubMed:1349837).Curated
Sequence conflicti573 – 5742GG → VV in CAA77646 (PubMed:1349837).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11547 mRNA. Translation: CAA77646.1.
AP001297 Genomic DNA. Translation: BAB03017.1.
CP002686 Genomic DNA. Translation: AEE76842.1.
AY099594 mRNA. Translation: AAM20445.1.
BT010398 mRNA. Translation: AAQ56841.1.
PIRiS20876.
RefSeqiNP_189041.1. NM_113304.4.
UniGeneiAt.25152.

Genome annotation databases

EnsemblPlantsiAT3G23990.1; AT3G23990.1; AT3G23990.
GeneIDi821983.
GrameneiAT3G23990.1; AT3G23990.1; AT3G23990.
KEGGiath:AT3G23990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11547 mRNA. Translation: CAA77646.1.
AP001297 Genomic DNA. Translation: BAB03017.1.
CP002686 Genomic DNA. Translation: AEE76842.1.
AY099594 mRNA. Translation: AAM20445.1.
BT010398 mRNA. Translation: AAQ56841.1.
PIRiS20876.
RefSeqiNP_189041.1. NM_113304.4.
UniGeneiAt.25152.

3D structure databases

ProteinModelPortaliP29197.
SMRiP29197. Positions 32-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7315. 5 interactions.
MINTiMINT-8065192.
STRINGi3702.AT3G23990.1.

PTM databases

iPTMnetiP29197.

Proteomic databases

PaxDbiP29197.
PRIDEiP29197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G23990.1; AT3G23990.1; AT3G23990.
GeneIDi821983.
GrameneiAT3G23990.1; AT3G23990.1; AT3G23990.
KEGGiath:AT3G23990.

Organism-specific databases

TAIRiAT3G23990.

Phylogenomic databases

eggNOGiKOG0356. Eukaryota.
COG0459. LUCA.
HOGENOMiHOG000076290.
InParanoidiP29197.
KOiK04077.
OMAiSNAMKKV.
PhylomeDBiP29197.

Miscellaneous databases

PROiP29197.

Gene expression databases

GenevisibleiP29197. AT.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60.
InterProiIPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiPS00296. CHAPERONINS_CPN60. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA clones encoding Arabidopsis thaliana and Zea mays mitochondrial chaperonin HSP60 and gene expression during seed germination and heat shock."
    Prasad T.K., Stewart C.R.
    Plant Mol. Biol. 18:873-885(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Seed.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  6. "Identification of phosphoproteins in Arabidopsis thaliana leaves using polyethylene glycol fractionation, immobilized metal-ion affinity chromatography, two-dimensional gel electrophoresis and mass spectrometry."
    Aryal U.K., Krochko J.E., Ross A.R.
    J. Proteome Res. 11:425-437(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCH60A_ARATH
AccessioniPrimary (citable) accession number: P29197
Secondary accession number(s): Q9LIQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 2000
Last modified: June 8, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.