P29193 (CAPP1_SACHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phosphoenolpyruvate carboxylase, housekeeping isozyme Short name=PEPC Short name=PEPCase EC=4.1.1.31
Organism	Saccharum hybrid (Sugarcane)
Taxonomic identifier	15819 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Saccharum

Protein attributes

Sequence length	966 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.
Cofactor	Magnesium By similarity.
Enzyme regulation	By light-reversible phosphorylation.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Photosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 966	966	Phosphoenolpyruvate carboxylase, housekeeping isozyme		PRO_0000166675
Sites
	Active site	172	1	By similarity
	Active site	601	1	By similarity
Amino acid modifications
	Modified residue	11	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29193 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: FD84762318FED073	FASTA	966	110,219
	10 20 30 40 50 60 MARNAVDKAT SIDAQLRLLA PQKLSDDDKL VEYDALLLDR FLDILQDLHG EDIRETVQEC 70 80 90 100 110 120 YELAAEYENK LDPKMLDEIG NVLTSLDPGD SIVITKSFSH MLILANLAEE VQIAYRRRIK 130 140 150 160 170 180 LKKGDFVDEN SATTESDIEE TLKRLMHQLK KSPLEVFDAL KNQTVDLVLT AHPTQSVRRS 190 200 210 220 230 240 LLQKHGRIRN CLTQLYAKDI TPDEKQELDE ALQREIQAAF RTDEIRRAPP TPQDEMRAGM 250 260 270 280 290 300 SYFHETIWKG VPKFLRRVDT ALKNIGINER LPYNAPIIQF SSWMGGDRDG NPRVTPEITR 310 320 330 340 350 360 DVCLLARMMA ANLYNAQIED LMFELSMWRC SDELRVKVDE LHRSSKKDTT KHYIEFWKQV 370 380 390 400 410 420 PPSEPYRVIL SDVRDKLYNT RERARHLLAS GFSEIPEEAT FTDVEQFLEP LELCYRSLCA 430 440 450 460 470 480 CGDRSVADGS LLDFLRQVST FGLSLVRLDI RQESDRHTDV MDAITEYLGI GSYRKWTEEK 490 500 510 520 530 540 RQEWLLSELN GKRPLFGPDL PKSDEIADVL DTFHVLAELP SDSFGAYVIS MATAPSDVLA 550 560 570 580 590 600 VELLQRECHV KKPLRVVPLF EKLADLEAAP AALARLFSVE WYRNRINGKQ EVMIGYSDSG 610 620 630 640 650 660 KDAGRFSAAW QLYKAQEELI NVAKLYGVKL TMFHGRGGTV GRGGGPTHLA ILSQPPETIH 670 680 690 700 710 720 GSLRVTVQGE VIEQSFGEEH LCFRTLQRFT AATLEHGMHP PISPKPEWRA LMDEMAIVAT 730 740 750 760 770 780 KEYRSIVFEE PRFVEYFRLA TPEMEYGRMN IGSRPSKRKP SAGIESLRAI PWIFAWTQTR 790 800 810 820 830 840 FHLPVWLGFG AAFKHVLDKD IRNLQTLQEM YNQWPFFRVT IDLVEMVFAK GDPGIAALYD 850 860 870 880 890 900 KLLVSEDLWS FGKRLRANYE ETKQLLLQVA GHKDLLEGDP YLKQRLRIRD SYITALNVCQ 910 920 930 940 950 960 AYMLKRIRDP GFQVNPGPHL SKDIMDMGKP ASELVKLNTT SEYAPGLEDT LILTMKGIAA GMQNTG

References

[1]	"Structure and expression of a sugarcane gene encoding a housekeeping phosphoenolpyruvate carboxylase." Albert H.A., Martin T., Sun S.S. Plant Mol. Biol. 20:663-671(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86661 Genomic DNA. Translation: AAC33164.1.
3D structure databases
ProteinModelPortal	P29193.
SMR	P29193. Positions 31-966.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os01t0758300-01.
Organism-specific databases
Gramene	P29193.
Family and domain databases
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP1_SACHY

Accession

Primary (citable) accession number: P29193

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program