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P29190 (PCKG_HAECO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEPCK
EC=4.1.1.32
Gene names
Name:PEPCK
OrganismHaemonchus contortus (Barber pole worm)
Taxonomic identifier6289 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaStrongylidaTrichostrongyloideaHaemonchidaeHaemonchinaeHaemonchus

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In parasitic nematodes PEPCK carboxylates phosphoenolpyruvate to oxaloacetate thus introducing the products of glycolysis to mitochondrial metabolism. HAMAP-Rule MF_00452

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Subunit structure

Monomer.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_0000103633

Regions

Nucleotide binding282 – 2876GTP By similarity
Nucleotide binding525 – 5284GTP By similarity
Region399 – 4013Substrate binding By similarity

Sites

Active site2831 By similarity
Metal binding2391Manganese By similarity
Metal binding2591Manganese; via tele nitrogen By similarity
Metal binding3061Manganese By similarity
Binding site811Substrate By similarity
Binding site2321Substrate; via amide nitrogen By similarity
Binding site2391Substrate By similarity
Binding site2811Substrate By similarity
Binding site4011GTP By similarity
Binding site4321GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
P29190 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 40A801A1EB647CFD

FASTA61969,656
        10         20         30         40         50         60 
MKRLGHVPIH KGDFHLLPPK VQRFVAEKAE LMRPRGIYIC DGSQHEADEI IDKLIERGML 

        70         80         90        100        110        120 
SPLKAYENNY ICRTDPKDVA RVESKTWMVT PDKYQTVCHT PDGIEPIMGH WMSPDSLATE 

       130        140        150        160        170        180 
LDSRFPGCMA GRIMYVIPFS MGPVGGPLSK IGVQLTDSNY VVLSMRIMTR VGHEVWDALG 

       190        200        210        220        230        240 
DNDFVRCIHS VGLPRPVKQR VINHWPCNPE RVLIAHRPAE REIWSFGSGY GGNSLLGKKM 

       250        260        270        280        290        300 
LALRIASNIA KDEGWMAEHM LIMGVTRPDG KEHFIAAAFP SACGKTNLAM LEPALPGWKV 

       310        320        330        340        350        360 
RCVGDDIAWM KFGEDGRLYA INPEYGFFGV APGTSKKTNP MAVATFQKNS IFTNVGETAN 

       370        380        390        400        410        420 
GEYFWEGLED EIKDKNVDMI NWLGEKWRIG DPGLCAHPNS RFAAPASQCP IIHPEWESPK 

       430        440        450        460        470        480 
GVPIDAIIFG GRRPAGVPLV FETRSWLHGI FTGACLKSEA TAAAEHKGKT VMHDPMAMRP 

       490        500        510        520        530        540 
FMGYNFGHYL QHWIDLNKDG RKVPKIYHVN WFRRDANNKF LWPGYGQNIR VIDWIVRRLD 

       550        560        570        580        590        600 
GEPDIGVDTP IGIVPKKGAI NASGLPDIQW DELMSVPKEY WTNDAKEIRK FLEEQVGPDL 

       610 
PKEIRAEMDA QEERINKQA 

« Hide

References

[1]"Cloning of a cDNA encoding phosphoenolpyruvate carboxykinase from Haemonchus contortus."
Klein R.D., Winterrowd C.A., Hatzenbuhler N.T., Shea M.H., Favreau M.A., Nulf S.C., Geary T.G.
Mol. Biochem. Parasitol. 50:285-294(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M76494 mRNA. Translation: AAA29180.1.
PIRA45625.

3D structure databases

ProteinModelPortalP29190.
SMRP29190. Positions 7-617.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_HAECO
AccessionPrimary (citable) accession number: P29190
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families