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P29190

- PCKG_HAECO

UniProt

P29190 - PCKG_HAECO

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
PEPCK
Organism
Haemonchus contortus (Barber pole worm)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

In parasitic nematodes PEPCK carboxylates phosphoenolpyruvate to oxaloacetate thus introducing the products of glycolysis to mitochondrial metabolism.UniRule annotation
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811Substrate By similarity
Binding sitei232 – 2321Substrate; via amide nitrogen By similarity
Metal bindingi239 – 2391Manganese By similarity
Binding sitei239 – 2391Substrate By similarity
Metal bindingi259 – 2591Manganese; via tele nitrogen By similarity
Binding sitei281 – 2811Substrate By similarity
Active sitei283 – 2831 By similarity
Metal bindingi306 – 3061Manganese By similarity
Binding sitei401 – 4011GTP By similarity
Binding sitei432 – 4321GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi282 – 2876GTP By similarity
Nucleotide bindingi525 – 5284GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
Gene namesi
Name:PEPCK
OrganismiHaemonchus contortus (Barber pole worm)
Taxonomic identifieri6289 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaStrongylidaTrichostrongyloideaHaemonchidaeHaemonchinaeHaemonchus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 619619Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_0000103633Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP29190.
SMRiP29190. Positions 7-617.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni399 – 4013Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29190-1 [UniParc]FASTAAdd to Basket

« Hide

MKRLGHVPIH KGDFHLLPPK VQRFVAEKAE LMRPRGIYIC DGSQHEADEI    50
IDKLIERGML SPLKAYENNY ICRTDPKDVA RVESKTWMVT PDKYQTVCHT 100
PDGIEPIMGH WMSPDSLATE LDSRFPGCMA GRIMYVIPFS MGPVGGPLSK 150
IGVQLTDSNY VVLSMRIMTR VGHEVWDALG DNDFVRCIHS VGLPRPVKQR 200
VINHWPCNPE RVLIAHRPAE REIWSFGSGY GGNSLLGKKM LALRIASNIA 250
KDEGWMAEHM LIMGVTRPDG KEHFIAAAFP SACGKTNLAM LEPALPGWKV 300
RCVGDDIAWM KFGEDGRLYA INPEYGFFGV APGTSKKTNP MAVATFQKNS 350
IFTNVGETAN GEYFWEGLED EIKDKNVDMI NWLGEKWRIG DPGLCAHPNS 400
RFAAPASQCP IIHPEWESPK GVPIDAIIFG GRRPAGVPLV FETRSWLHGI 450
FTGACLKSEA TAAAEHKGKT VMHDPMAMRP FMGYNFGHYL QHWIDLNKDG 500
RKVPKIYHVN WFRRDANNKF LWPGYGQNIR VIDWIVRRLD GEPDIGVDTP 550
IGIVPKKGAI NASGLPDIQW DELMSVPKEY WTNDAKEIRK FLEEQVGPDL 600
PKEIRAEMDA QEERINKQA 619
Length:619
Mass (Da):69,656
Last modified:December 1, 1992 - v1
Checksum:i40A801A1EB647CFD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76494 mRNA. Translation: AAA29180.1.
PIRiA45625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76494 mRNA. Translation: AAA29180.1 .
PIRi A45625.

3D structure databases

ProteinModelPortali P29190.
SMRi P29190. Positions 7-617.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a cDNA encoding phosphoenolpyruvate carboxykinase from Haemonchus contortus."
    Klein R.D., Winterrowd C.A., Hatzenbuhler N.T., Shea M.H., Favreau M.A., Nulf S.C., Geary T.G.
    Mol. Biochem. Parasitol. 50:285-294(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPCKG_HAECO
AccessioniPrimary (citable) accession number: P29190
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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