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P29190

- PCKG_HAECO

UniProt

P29190 - PCKG_HAECO

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

PEPCK

Organism
Haemonchus contortus (Barber pole worm)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    In parasitic nematodes PEPCK carboxylates phosphoenolpyruvate to oxaloacetate thus introducing the products of glycolysis to mitochondrial metabolism.
    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei232 – 2321Substrate; via amide nitrogenBy similarity
    Metal bindingi239 – 2391ManganeseBy similarity
    Binding sitei239 – 2391SubstrateBy similarity
    Metal bindingi259 – 2591Manganese; via tele nitrogenBy similarity
    Binding sitei281 – 2811SubstrateBy similarity
    Active sitei283 – 2831By similarity
    Metal bindingi306 – 3061ManganeseBy similarity
    Binding sitei401 – 4011GTPBy similarity
    Binding sitei432 – 4321GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi282 – 2876GTPBy similarity
    Nucleotide bindingi525 – 5284GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

    GO - Biological processi

    1. gluconeogenesis Source: InterPro

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
    Short name:
    PEPCK
    Gene namesi
    Name:PEPCK
    OrganismiHaemonchus contortus (Barber pole worm)
    Taxonomic identifieri6289 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaStrongylidaTrichostrongyloideaHaemonchidaeHaemonchinaeHaemonchus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 619619Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103633Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP29190.
    SMRiP29190. Positions 7-617.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni399 – 4013Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29190-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRLGHVPIH KGDFHLLPPK VQRFVAEKAE LMRPRGIYIC DGSQHEADEI    50
    IDKLIERGML SPLKAYENNY ICRTDPKDVA RVESKTWMVT PDKYQTVCHT 100
    PDGIEPIMGH WMSPDSLATE LDSRFPGCMA GRIMYVIPFS MGPVGGPLSK 150
    IGVQLTDSNY VVLSMRIMTR VGHEVWDALG DNDFVRCIHS VGLPRPVKQR 200
    VINHWPCNPE RVLIAHRPAE REIWSFGSGY GGNSLLGKKM LALRIASNIA 250
    KDEGWMAEHM LIMGVTRPDG KEHFIAAAFP SACGKTNLAM LEPALPGWKV 300
    RCVGDDIAWM KFGEDGRLYA INPEYGFFGV APGTSKKTNP MAVATFQKNS 350
    IFTNVGETAN GEYFWEGLED EIKDKNVDMI NWLGEKWRIG DPGLCAHPNS 400
    RFAAPASQCP IIHPEWESPK GVPIDAIIFG GRRPAGVPLV FETRSWLHGI 450
    FTGACLKSEA TAAAEHKGKT VMHDPMAMRP FMGYNFGHYL QHWIDLNKDG 500
    RKVPKIYHVN WFRRDANNKF LWPGYGQNIR VIDWIVRRLD GEPDIGVDTP 550
    IGIVPKKGAI NASGLPDIQW DELMSVPKEY WTNDAKEIRK FLEEQVGPDL 600
    PKEIRAEMDA QEERINKQA 619
    Length:619
    Mass (Da):69,656
    Last modified:December 1, 1992 - v1
    Checksum:i40A801A1EB647CFD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76494 mRNA. Translation: AAA29180.1.
    PIRiA45625.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76494 mRNA. Translation: AAA29180.1 .
    PIRi A45625.

    3D structure databases

    ProteinModelPortali P29190.
    SMRi P29190. Positions 7-617.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a cDNA encoding phosphoenolpyruvate carboxykinase from Haemonchus contortus."
      Klein R.D., Winterrowd C.A., Hatzenbuhler N.T., Shea M.H., Favreau M.A., Nulf S.C., Geary T.G.
      Mol. Biochem. Parasitol. 50:285-294(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiPCKG_HAECO
    AccessioniPrimary (citable) accession number: P29190
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3