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Protein

Pancreatic triacylglycerol lipase

Gene

PNLIP

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651Nucleophile
Active sitei189 – 1891Charge relay system
Metal bindingi200 – 2001Calcium; via carbonyl oxygen
Metal bindingi203 – 2031Calcium; via carbonyl oxygen
Metal bindingi205 – 2051Calcium
Metal bindingi208 – 2081Calcium
Active sitei276 – 2761Charge relay system

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. triglyceride lipase activity Source: UniProtKB-EC

GO - Biological processi

  1. intestinal cholesterol absorption Source: Ensembl
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic triacylglycerol lipase (EC:3.1.1.3)
Short name:
PL
Short name:
PTL
Short name:
Pancreatic lipase
Gene namesi
Name:PNLIP
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi165 – 1651S → X: 80% loss of P-nitrophenyl acetate hydrolysis activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 12›12Add
BLAST
Chaini13 – 461449Pancreatic triacylglycerol lipasePRO_0000017784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi16 ↔ 22
Disulfide bondi103 ↔ 114
Disulfide bondi250 ↔ 274
Disulfide bondi298 ↔ 309
Disulfide bondi312 ↔ 317
Disulfide bondi445 ↔ 461

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By colipase/CLPS in the presence of bile salts.

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000007877.

Structurei

Secondary structure

1
461
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 174Combined sources
Turni18 – 203Combined sources
Beta strandi21 – 244Combined sources
Beta strandi29 – 324Combined sources
Helixi44 – 474Combined sources
Beta strandi50 – 556Combined sources
Beta strandi58 – 658Combined sources
Helixi69 – 746Combined sources
Beta strandi81 – 877Combined sources
Helixi98 – 10912Combined sources
Beta strandi112 – 1187Combined sources
Helixi120 – 1234Combined sources
Helixi127 – 15226Combined sources
Helixi156 – 1583Combined sources
Beta strandi159 – 1646Combined sources
Helixi167 – 17711Combined sources
Turni178 – 1803Combined sources
Beta strandi182 – 1898Combined sources
Turni193 – 1975Combined sources
Turni200 – 2023Combined sources
Helixi206 – 2083Combined sources
Beta strandi209 – 2157Combined sources
Helixi222 – 2254Combined sources
Beta strandi235 – 2417Combined sources
Helixi261 – 2655Combined sources
Helixi274 – 28815Combined sources
Helixi301 – 3055Combined sources
Beta strandi319 – 3213Combined sources
Helixi324 – 3263Combined sources
Turni328 – 3314Combined sources
Beta strandi332 – 3409Combined sources
Beta strandi344 – 3474Combined sources
Beta strandi351 – 36111Combined sources
Beta strandi364 – 37411Combined sources
Beta strandi382 – 3898Combined sources
Beta strandi394 – 40310Combined sources
Beta strandi407 – 41711Combined sources
Beta strandi427 – 4359Combined sources
Beta strandi441 – 4455Combined sources
Beta strandi456 – 4616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HPLX-ray2.30A/B13-461[»]
ProteinModelPortaliP29183.
SMRiP29183. Positions 13-461.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29183.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini351 – 461111PLATPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40923.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP29183.
KOiK14073.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR008976. Lipase_LipOase.
IPR013818. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
WTLSLLLGAV VGNEVCYERL GCFSDDSPWA GIVERPLKIL PWSPEKVNTR
60 70 80 90 100
FLLYTNENPD NFQEIVADPS TIQSSNFNTG RKTRFIIHGF IDKGEESWLS
110 120 130 140 150
TMCQNMFKVE SVNCICVDWK SGSRTAYSQA SQNVRIVGAE VAYLVGVLQS
160 170 180 190 200
SFDYSPSNVH IIGHSLGSHA AGEAGRRTNG AVGRITGLDP AEPCFQGTPE
210 220 230 240 250
LVRLDPSDAQ FVDVIHTDIA PFIPNLGFGM SQTAGHLDFF PNGGKEMPGC
260 270 280 290 300
QKNVLSQIVD IDGIWQGTRD FAACNHLRSY KYYTDSILNP DGFAGFSCAS
310 320 330 340 350
YSDFTANKCF PCSSEGCPQM GHYADRFPGR TKGVGQLFYL NTGDASNFAR
360 370 380 390 400
WRYRVDVTLS GKKVTGHVLV SLFGNKGNSR QYEIFQGTLK PDNTYSNEFD
410 420 430 440 450
SDVEVGDLEK VKFIWYNNVI NLTLPKVGAS KITVERNDGS VFNFCSEETV
460
REDVLLTLTA C
Length:461
Mass (Da):50,921
Last modified:April 1, 1993 - v2
Checksum:i382F33F3CE446738
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66218 mRNA. Translation: CAA46961.1.
PIRiS21223.
RefSeqiNP_001157421.1. NM_001163949.1.
UniGeneiEca.12462.

Genome annotation databases

GeneIDi100034202.
KEGGiecb:100034202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66218 mRNA. Translation: CAA46961.1.
PIRiS21223.
RefSeqiNP_001157421.1. NM_001163949.1.
UniGeneiEca.12462.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HPLX-ray2.30A/B13-461[»]
ProteinModelPortaliP29183.
SMRiP29183. Positions 13-461.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000007877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100034202.
KEGGiecb:100034202.

Organism-specific databases

CTDi5406.

Phylogenomic databases

eggNOGiNOG40923.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP29183.
KOiK14073.

Miscellaneous databases

EvolutionaryTraceiP29183.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR008976. Lipase_LipOase.
IPR013818. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of horse pancreatic lipase as determined by protein and cDNA sequencing. Implications for p-nitrophenyl acetate hydrolysis by pancreatic lipases."
    Kerfelec B., Foglizzo E., Bonicel J., Bougis P.E., Chapus C.
    Eur. J. Biochem. 206:279-287(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Horse pancreatic lipase. The crystal structure refined at 2.3-A resolution."
    Bourne Y., Martinez C., Kerfelec B., Lombardo D., Chapus C., Cambillau C.
    J. Mol. Biol. 238:709-732(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiLIPP_HORSE
AccessioniPrimary (citable) accession number: P29183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: April 1, 1993
Last modified: January 7, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.