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Protein

Pancreatic triacylglycerol lipase

Gene

PNLIP

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei165Nucleophile1
Active sitei189Charge relay system1
Metal bindingi200Calcium; via carbonyl oxygen1
Metal bindingi203Calcium; via carbonyl oxygen1
Metal bindingi205Calcium1
Metal bindingi208Calcium1
Active sitei276Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

ESTHERihorse-1plip. Pancreatic_lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic triacylglycerol lipase (EC:3.1.1.3)
Short name:
PL
Short name:
PTL
Short name:
Pancreatic lipase
Gene namesi
Name:PNLIP
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi165S → X: 80% loss of P-nitrophenyl acetate hydrolysis activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1 – 12Add BLAST›12
ChainiPRO_000001778413 – 461Pancreatic triacylglycerol lipaseAdd BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi16 ↔ 22
Disulfide bondi103 ↔ 114
Disulfide bondi250 ↔ 274
Disulfide bondi298 ↔ 309
Disulfide bondi312 ↔ 317
Disulfide bondi445 ↔ 461

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP29183.
PRIDEiP29183.

Expressioni

Inductioni

By colipase/CLPS in the presence of bile salts.

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000007877.

Structurei

Secondary structure

1461
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 17Combined sources4
Turni18 – 20Combined sources3
Beta strandi21 – 24Combined sources4
Beta strandi29 – 32Combined sources4
Helixi44 – 47Combined sources4
Beta strandi50 – 55Combined sources6
Beta strandi58 – 65Combined sources8
Helixi69 – 74Combined sources6
Beta strandi81 – 87Combined sources7
Helixi98 – 109Combined sources12
Beta strandi112 – 118Combined sources7
Helixi120 – 123Combined sources4
Helixi127 – 152Combined sources26
Helixi156 – 158Combined sources3
Beta strandi159 – 164Combined sources6
Helixi167 – 177Combined sources11
Turni178 – 180Combined sources3
Beta strandi182 – 189Combined sources8
Turni193 – 197Combined sources5
Turni200 – 202Combined sources3
Helixi206 – 208Combined sources3
Beta strandi209 – 215Combined sources7
Helixi222 – 225Combined sources4
Beta strandi235 – 241Combined sources7
Helixi261 – 265Combined sources5
Helixi274 – 288Combined sources15
Helixi301 – 305Combined sources5
Beta strandi319 – 321Combined sources3
Helixi324 – 326Combined sources3
Turni328 – 331Combined sources4
Beta strandi332 – 340Combined sources9
Beta strandi344 – 347Combined sources4
Beta strandi351 – 361Combined sources11
Beta strandi364 – 374Combined sources11
Beta strandi382 – 389Combined sources8
Beta strandi394 – 403Combined sources10
Beta strandi407 – 417Combined sources11
Beta strandi427 – 435Combined sources9
Beta strandi441 – 445Combined sources5
Beta strandi456 – 461Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HPLX-ray2.30A/B13-461[»]
ProteinModelPortaliP29183.
SMRiP29183.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29183.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini351 – 461PLATPROSITE-ProRule annotationAdd BLAST111

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHRX. Eukaryota.
ENOG410Y92X. LUCA.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP29183.
KOiK14073.

Family and domain databases

CDDicd00707. Pancreat_lipase_like. 1 hit.
Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR013818. Lipase/vitellogenin.
IPR016272. Lipase_LIPH.
IPR033906. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
WTLSLLLGAV VGNEVCYERL GCFSDDSPWA GIVERPLKIL PWSPEKVNTR
60 70 80 90 100
FLLYTNENPD NFQEIVADPS TIQSSNFNTG RKTRFIIHGF IDKGEESWLS
110 120 130 140 150
TMCQNMFKVE SVNCICVDWK SGSRTAYSQA SQNVRIVGAE VAYLVGVLQS
160 170 180 190 200
SFDYSPSNVH IIGHSLGSHA AGEAGRRTNG AVGRITGLDP AEPCFQGTPE
210 220 230 240 250
LVRLDPSDAQ FVDVIHTDIA PFIPNLGFGM SQTAGHLDFF PNGGKEMPGC
260 270 280 290 300
QKNVLSQIVD IDGIWQGTRD FAACNHLRSY KYYTDSILNP DGFAGFSCAS
310 320 330 340 350
YSDFTANKCF PCSSEGCPQM GHYADRFPGR TKGVGQLFYL NTGDASNFAR
360 370 380 390 400
WRYRVDVTLS GKKVTGHVLV SLFGNKGNSR QYEIFQGTLK PDNTYSNEFD
410 420 430 440 450
SDVEVGDLEK VKFIWYNNVI NLTLPKVGAS KITVERNDGS VFNFCSEETV
460
REDVLLTLTA C
Length:461
Mass (Da):50,921
Last modified:April 1, 1993 - v2
Checksum:i382F33F3CE446738
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66218 mRNA. Translation: CAA46961.1.
PIRiS21223.
RefSeqiNP_001157421.1. NM_001163949.1.
UniGeneiEca.12462.

Genome annotation databases

GeneIDi100034202.
KEGGiecb:100034202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66218 mRNA. Translation: CAA46961.1.
PIRiS21223.
RefSeqiNP_001157421.1. NM_001163949.1.
UniGeneiEca.12462.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HPLX-ray2.30A/B13-461[»]
ProteinModelPortaliP29183.
SMRiP29183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000007877.

Protein family/group databases

ESTHERihorse-1plip. Pancreatic_lipase.

Proteomic databases

PaxDbiP29183.
PRIDEiP29183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100034202.
KEGGiecb:100034202.

Organism-specific databases

CTDi5406.

Phylogenomic databases

eggNOGiENOG410IHRX. Eukaryota.
ENOG410Y92X. LUCA.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP29183.
KOiK14073.

Miscellaneous databases

EvolutionaryTraceiP29183.

Family and domain databases

CDDicd00707. Pancreat_lipase_like. 1 hit.
Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR013818. Lipase/vitellogenin.
IPR016272. Lipase_LIPH.
IPR033906. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIPP_HORSE
AccessioniPrimary (citable) accession number: P29183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.