Reviewed, UniProtKB/Swiss-Prot P29177 (MGMT_BOVIN)

Names and origin

Protein names

Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    EC=2.1.1.63
Alternative name(s):
    6-O-methylguanine-DNA methyltransferase
      Short name=MGMT
    O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:

MGMT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	9 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›9	›9	Methylated-DNA--protein-cysteine methyltransferase		PRO_0000139356
Sites
	Active site	9	1	Nucleophile; methyl group acceptor By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	9	1

Sequences

	Sequence		Length	Mass (Da)
	P29177-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 325171A720476047	FASTA	9	967
	NPIPILTPC

References

[1]	"Active site amino acid sequence of the bovine O6-methylguanine-DNA methyltransferase." Rydberg B., Hall J., Karran P. Nucleic Acids Res. 18:17-21(1990) [PubMed: 2308822] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Thymus.

Cross-references

Sequence databases
IPI	IPI00694323.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P29177.
Enzyme and pathway databases
BRENDA	2.1.1.63. 251.
Family and domain databases
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. [Graphical view]
PROSITE	PS00374. MGMT. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGMT_BOVIN

Accession

Primary (citable) accession number: P29177

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)