Reviewed,
UniProtKB/Swiss-Prot P29177 (MGMT_BOVIN)
Last modified
June 16, 2009.
Version 42.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Methylated-DNA--protein-cysteine methyltransferase EC=2.1.1.63 Alternative name(s): 6-O-methylguanine-DNA methyltransferase Short name=MGMT O-6-methylguanine-DNA-alkyltransferase | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 9 AA. |
| Sequence status | Fragment. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Catalytic activity | DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. |
| Cofactor | Binds 1 zinc ion By similarity. |
| Subcellular location | Nucleus Probable. |
| Sequence similarities | Belongs to the MGMT family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair |
| Cellular component | Nucleus |
| Ligand | DNA-binding Metal-binding Zinc |
| Molecular function | Methyltransferase Transferase |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | DNA repair Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated-DNA-[protein]-cysteine S-methyltransferase activityInferred from electronic annotation. Source: EC zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
References
| [1] | "Active site amino acid sequence of the bovine O6-methylguanine-DNA methyltransferase." Rydberg B., Hall J., Karran P. Nucleic Acids Res. 18:17-21(1990) [PubMed: 2308822] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Thymus. |
Cross-references
Sequence databases | |
|---|---|
| IPI | IPI00694323. |
3D structure databases | |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | P29177. |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.63. 251. |
Family and domain databases | |
| InterPro | IPR001497. MethylDNA_cys_MeTrfase_AS. [Graphical view] |
| PROSITE | PS00374. MGMT. Partial match. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MGMT_BOVIN | ||||||||
| Accession | Primary (citable) accession number: P29177 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


