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P29168

- GAG_MLVDE

UniProt

P29168 - GAG_MLVDE

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Protein

Gag polyprotein

Gene
gag
Organism
Murine leukemia virus (strain DEF27)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei129 – 1302Cleavage; by viral protease p14 By similarity
Sitei213 – 2142Cleavage; by viral protease p14 By similarity
Sitei476 – 4772Cleavage; by viral protease p14 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri500 – 51718CCHC-typeAdd
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. virion assembly Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiMurine leukemia virus (strain DEF27)
Taxonomic identifieri31688 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

Chain Gag polyprotein : Virion By similarity. Host cell membrane; Lipid-anchor Reviewed prediction
Chain Matrix protein p15 : Virion Reviewed prediction
Chain Capsid protein p30 : Virion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 536535Gag polyprotein By similarityPRO_0000390809Add
BLAST
Chaini2 – 129128Matrix protein p15 Reviewed predictionPRO_0000040888Add
BLAST
Chaini130 – 21384RNA-binding phosphoprotein p12 Reviewed predictionPRO_0000040889Add
BLAST
Chaini214 – 476263Capsid protein p30 Reviewed predictionPRO_0000040890Add
BLAST
Chaini477 – 53660Nucleocapsid protein p10 Reviewed predictionPRO_0000040891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host By similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Structurei

3D structure databases

ProteinModelPortaliP29168.
SMRiP29168. Positions 2-98, 214-345, 350-380, 477-532.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili436 – 47439 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1864PPXY motif Reviewed prediction

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase Reviewed prediction.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri500 – 51718CCHC-typeAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29168-1 [UniParc]FASTAAdd to Basket

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MGQTITTPLS LTLEHWRDVQ CIASNQSVDV KRRRWVTFCS VEWPSFDVGW    50
PLDGTFNLDI ILQVKSKVFC PGPHGHPDQV PYIVTWEALA YHPPPWVKPF 100
VSPKPFPLST LPFSPPGPSA HPPSRSDLYT ALIPSIKTKP PKSRVLPTNG 150
GPLIDLLTEN PPNLGEQGPP LPKGPVKKRR PPPPRYSPPG PMVSRLRGNR 200
DPPAADSTTS RAFPLRLGGN GQLQYWPFSS SDLYNWKNNN PSFSEDPGKL 250
TALIESVLTT HQPTWDDCQQ LLGTLLTGEE KQRVLLEARK AVRGNDGRPT 300
QLPNEVNSAF PLERPDWDYT TPEGRNHLVL YRQLLLAGLQ NAGRSPTNLA 350
KVKGITQGPS ESPSAFLERL KEAYRRYTPY DPEDPGQETN VSMSFIWQSA 400
PDIGRKLERL EDLKSKTLGD LVREAEKIFN KRETPEEREE RIRRETEEKE 450
ERRRAEDEQR EKERDRRRHR EMSKFLATVV TGQRQDRQGG ERRRPQLDKD 500
QCAYCKEKGH WAKDCPKKPR GPRGPRPQTS LLTLGD 536
Length:536
Mass (Da):60,891
Last modified:January 23, 2007 - v3
Checksum:i4D990FD5C5AEF39B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64096 Genomic DNA. Translation: AAA46509.1. Sequence problems.
PIRiB40416. FOMVME.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64096 Genomic DNA. Translation: AAA46509.1 . Sequence problems.
PIRi B40416. FOMVME.

3D structure databases

ProteinModelPortali P29168.
SMRi P29168. Positions 2-98, 214-345, 350-380, 477-532.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEi PS50158. ZF_CCHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characteristics and contributions of defective, ecotropic, and mink cell focus-inducing viruses involved in a retrovirus-induced immunodeficiency syndrome of mice."
    Chattopadhyay S.K., Sengupta D.N., Fredrickson T.N., Morse H.C. III, Hartley J.W.
    J. Virol. 65:4232-4241(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGAG_MLVDE
AccessioniPrimary (citable) accession number: P29168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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