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P29168

- GAG_MLVDE

UniProt

P29168 - GAG_MLVDE

Protein

Gag polyprotein

Gene

gag

Organism
Murine leukemia virus (strain DEF27)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.By similarity
    Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
    Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei129 – 1302Cleavage; by viral protease p14By similarity
    Sitei213 – 2142Cleavage; by viral protease p14By similarity
    Sitei476 – 4772Cleavage; by viral protease p14By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri500 – 51718CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural constituent of virion Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. virion assembly Source: InterPro

    Keywords - Ligandi

    Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gag polyprotein
    Alternative name(s):
    Core polyprotein
    Cleaved into the following 4 chains:
    Matrix protein p15
    Short name:
    MA
    Alternative name(s):
    pp12
    Capsid protein p30
    Short name:
    CA
    Nucleocapsid protein p10
    Short name:
    NC-gag
    Gene namesi
    Name:gag
    OrganismiMurine leukemia virus (strain DEF27)
    Taxonomic identifieri31688 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-KW
    3. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by hostBy similarity
    Chaini2 – 536535Gag polyproteinBy similarityPRO_0000390809Add
    BLAST
    Chaini2 – 129128Matrix protein p15Sequence AnalysisPRO_0000040888Add
    BLAST
    Chaini130 – 21384RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040889Add
    BLAST
    Chaini214 – 476263Capsid protein p30Sequence AnalysisPRO_0000040890Add
    BLAST
    Chaini477 – 53660Nucleocapsid protein p10Sequence AnalysisPRO_0000040891Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Interactioni

    Subunit structurei

    Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP29168.
    SMRiP29168. Positions 2-98, 214-345, 350-380, 477-532.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili436 – 47439Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi183 – 1864PPXY motifSequence Analysis

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase Potential.Curated

    Sequence similaritiesi

    Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri500 – 51718CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Family and domain databases

    Gene3Di1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProiIPR000840. G_retro_matrix_N.
    IPR002079. Gag_p12.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01140. Gag_MA. 1 hit.
    PF01141. Gag_p12. 1 hit.
    PF02093. Gag_p30. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEiPS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29168-1 [UniParc]FASTAAdd to Basket

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    MGQTITTPLS LTLEHWRDVQ CIASNQSVDV KRRRWVTFCS VEWPSFDVGW    50
    PLDGTFNLDI ILQVKSKVFC PGPHGHPDQV PYIVTWEALA YHPPPWVKPF 100
    VSPKPFPLST LPFSPPGPSA HPPSRSDLYT ALIPSIKTKP PKSRVLPTNG 150
    GPLIDLLTEN PPNLGEQGPP LPKGPVKKRR PPPPRYSPPG PMVSRLRGNR 200
    DPPAADSTTS RAFPLRLGGN GQLQYWPFSS SDLYNWKNNN PSFSEDPGKL 250
    TALIESVLTT HQPTWDDCQQ LLGTLLTGEE KQRVLLEARK AVRGNDGRPT 300
    QLPNEVNSAF PLERPDWDYT TPEGRNHLVL YRQLLLAGLQ NAGRSPTNLA 350
    KVKGITQGPS ESPSAFLERL KEAYRRYTPY DPEDPGQETN VSMSFIWQSA 400
    PDIGRKLERL EDLKSKTLGD LVREAEKIFN KRETPEEREE RIRRETEEKE 450
    ERRRAEDEQR EKERDRRRHR EMSKFLATVV TGQRQDRQGG ERRRPQLDKD 500
    QCAYCKEKGH WAKDCPKKPR GPRGPRPQTS LLTLGD 536
    Length:536
    Mass (Da):60,891
    Last modified:January 23, 2007 - v3
    Checksum:i4D990FD5C5AEF39B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64096 Genomic DNA. Translation: AAA46509.1. Sequence problems.
    PIRiB40416. FOMVME.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64096 Genomic DNA. Translation: AAA46509.1 . Sequence problems.
    PIRi B40416. FOMVME.

    3D structure databases

    ProteinModelPortali P29168.
    SMRi P29168. Positions 2-98, 214-345, 350-380, 477-532.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProi IPR000840. G_retro_matrix_N.
    IPR002079. Gag_p12.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01140. Gag_MA. 1 hit.
    PF01141. Gag_p12. 1 hit.
    PF02093. Gag_p30. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEi PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characteristics and contributions of defective, ecotropic, and mink cell focus-inducing viruses involved in a retrovirus-induced immunodeficiency syndrome of mice."
      Chattopadhyay S.K., Sengupta D.N., Fredrickson T.N., Morse H.C. III, Hartley J.W.
      J. Virol. 65:4232-4241(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGAG_MLVDE
    AccessioniPrimary (citable) accession number: P29168
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 84 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3