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P29168 (GAG_MLVDE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein

Cleaved into the following 4 chains:

  1. Matrix protein p15
    Short name=MA
  2. RNA-binding phosphoprotein p12
    Alternative name(s):
    pp12
  3. Capsid protein p30
    Short name=CA
  4. Nucleocapsid protein p10
    Short name=NC-gag
Gene names
Name:gag
OrganismMurine leukemia virus (strain DEF27)
Taxonomic identifier31688 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.

Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.

Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.

Subunit structure

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Subcellular location

Gag polyprotein: Virion By similarity. Host cell membrane; Lipid-anchor Potential.

Matrix protein p15: Virion Potential.

Capsid protein p30: Virion Potential.

Nucleocapsid protein p10: Virion Potential.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase Potential.

Post-translational modification

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Sequence similarities

Contains 1 CCHC-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host By similarity
Chain2 – 536535Gag polyprotein By similarity
PRO_0000390809
Chain2 – 129128Matrix protein p15 Potential
PRO_0000040888
Chain130 – 21384RNA-binding phosphoprotein p12 Potential
PRO_0000040889
Chain214 – 476263Capsid protein p30 Potential
PRO_0000040890
Chain477 – 53660Nucleocapsid protein p10 Potential
PRO_0000040891

Regions

Zinc finger500 – 51718CCHC-type
Coiled coil436 – 47439 Potential
Motif183 – 1864PPXY motif Potential

Sites

Site129 – 1302Cleavage; by viral protease p14 By similarity
Site213 – 2142Cleavage; by viral protease p14 By similarity
Site476 – 4772Cleavage; by viral protease p14 By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine; by host By similarity

Sequences

Sequence LengthMass (Da)Tools
P29168 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 4D990FD5C5AEF39B

FASTA53660,891
        10         20         30         40         50         60 
MGQTITTPLS LTLEHWRDVQ CIASNQSVDV KRRRWVTFCS VEWPSFDVGW PLDGTFNLDI 

        70         80         90        100        110        120 
ILQVKSKVFC PGPHGHPDQV PYIVTWEALA YHPPPWVKPF VSPKPFPLST LPFSPPGPSA 

       130        140        150        160        170        180 
HPPSRSDLYT ALIPSIKTKP PKSRVLPTNG GPLIDLLTEN PPNLGEQGPP LPKGPVKKRR 

       190        200        210        220        230        240 
PPPPRYSPPG PMVSRLRGNR DPPAADSTTS RAFPLRLGGN GQLQYWPFSS SDLYNWKNNN 

       250        260        270        280        290        300 
PSFSEDPGKL TALIESVLTT HQPTWDDCQQ LLGTLLTGEE KQRVLLEARK AVRGNDGRPT 

       310        320        330        340        350        360 
QLPNEVNSAF PLERPDWDYT TPEGRNHLVL YRQLLLAGLQ NAGRSPTNLA KVKGITQGPS 

       370        380        390        400        410        420 
ESPSAFLERL KEAYRRYTPY DPEDPGQETN VSMSFIWQSA PDIGRKLERL EDLKSKTLGD 

       430        440        450        460        470        480 
LVREAEKIFN KRETPEEREE RIRRETEEKE ERRRAEDEQR EKERDRRRHR EMSKFLATVV 

       490        500        510        520        530 
TGQRQDRQGG ERRRPQLDKD QCAYCKEKGH WAKDCPKKPR GPRGPRPQTS LLTLGD 

« Hide

References

[1]"Characteristics and contributions of defective, ecotropic, and mink cell focus-inducing viruses involved in a retrovirus-induced immunodeficiency syndrome of mice."
Chattopadhyay S.K., Sengupta D.N., Fredrickson T.N., Morse H.C. III, Hartley J.W.
J. Virol. 65:4232-4241(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64096 Genomic DNA. Translation: AAA46509.1. Sequence problems.
PIRFOMVME. B40416.

3D structure databases

ProteinModelPortalP29168.
SMRP29168. Positions 2-98, 214-345, 350-380, 477-532.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAG_MLVDE
AccessionPrimary (citable) accession number: P29168
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families