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Protein

Bacillolysin

Gene

npr

Organism
Paenibacillus polymyxa (Bacillus polymyxa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the generation of beta- and alpha-amylases from the large amylase precursor.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca2+ ions per subunit.Curated
  • Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi339Calcium 1Sequence analysis1
Metal bindingi341Calcium 1Sequence analysis1
Metal bindingi419Calcium 2Sequence analysis1
Metal bindingi423Zinc; catalyticPROSITE-ProRule annotation1
Active sitei424PROSITE-ProRule annotation1
Metal bindingi427Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi447Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi466Calcium 2Sequence analysis1
Metal bindingi466Calcium 3Sequence analysis1
Metal bindingi469Calcium 4; via carbonyl oxygenSequence analysis1
Metal bindingi470Calcium 4Sequence analysis1
Metal bindingi473Calcium 4; via carbonyl oxygenSequence analysis1
Metal bindingi476Calcium 4Sequence analysis1
Active sitei507Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
OrganismiPaenibacillus polymyxa (Bacillus polymyxa)
Taxonomic identifieri1406 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002860225 – 286Activation peptide1 PublicationAdd BLAST262
ChainiPRO_0000028603287 – 590BacillolysinAdd BLAST304

Keywords - PTMi

Zymogen

Interactioni

Protein-protein interaction databases

STRINGi886882.PPSC2_c4320.

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi290 – 292Combined sources3
Beta strandi298 – 302Combined sources5
Beta strandi304 – 306Combined sources3
Beta strandi309 – 314Combined sources6
Beta strandi321 – 325Combined sources5
Beta strandi338 – 344Combined sources7
Helixi347 – 367Combined sources21
Turni371 – 373Combined sources3
Beta strandi379 – 388Combined sources10
Beta strandi392 – 394Combined sources3
Beta strandi399 – 402Combined sources4
Beta strandi408 – 411Combined sources4
Helixi414 – 416Combined sources3
Helixi418 – 431Combined sources14
Turni432 – 434Combined sources3
Helixi440 – 458Combined sources19
Beta strandi460 – 465Combined sources6
Helixi466 – 468Combined sources3
Beta strandi478 – 482Combined sources5
Helixi484 – 487Combined sources4
Helixi493 – 495Combined sources3
Helixi501 – 522Combined sources22
Beta strandi524 – 526Combined sources3
Beta strandi529 – 531Combined sources3
Helixi536 – 549Combined sources14
Helixi557 – 572Combined sources16
Helixi577 – 589Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GERX-ray1.59A/B290-590[»]
ProteinModelPortaliP29148.
SMRiP29148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105D4Y. Bacteria.
COG3227. LUCA.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVWFSLLG GAMLLGSVAS GASAESSVSG PAQLTPTFHT EQWKAPSSVS
60 70 80 90 100
GDDIVWSYLN RQKKSLLGVD SSSVREQFRI VDRTSDKSGV SHYRLKQYVN
110 120 130 140 150
GIPVYGAEQT IHVGKSGEVT SYLGAVINED QQEEATQGTT PKISASEAVY
160 170 180 190 200
TAYKEAAARI EALPTSDDTI SKDAEEPSSV SKDTYAEAAN NDKTLSVDKD
210 220 230 240 250
ELSLDKASVL KDSKIEAVEA EKSSIAKIAN LQPEVDPKAE LYYYPKGDDL
260 270 280 290 300
LLVYVTEVNV LEPAPLRTRY IIDANDGSIV FQYDIINEAT GKGVLGDSKS
310 320 330 340 350
FTTTASGSSY QLKDTTRGNG IVTYTASNRQ SIPGTLLTDA DNVWNDPAGV
360 370 380 390 400
DAHAYAAKTY DYYKSKFGRN SIDGRGLQLR STVHYGSRYN NAFWNGSQMT
410 420 430 440 450
YGDGDGDGST FIAFSGDPDV VGHELTHGVT EYTSNLEYYG ESGALNEAFS
460 470 480 490 500
DVIGNDIQRK NWLVGDDIYT PNICGDALRS MSNPTLYDQP HHYSNLYKGS
510 520 530 540 550
SDNGGVHTNS GIINKAYYLL AQGGTFHGVT VNGIGRDAAV QIYYSAFTNY
560 570 580 590
LTSSSDFSNA RAAVIQAAKD LYGANSAEAT AAAKSFDAVG
Length:590
Mass (Da):63,529
Last modified:December 1, 1992 - v1
Checksum:i4ED303761408F6F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti287 – 289NEA → ATG AA sequence (PubMed:1834632).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00861 Genomic DNA. Translation: BAA00734.1.
PIRiA41335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00861 Genomic DNA. Translation: BAA00734.1.
PIRiA41335.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GERX-ray1.59A/B290-590[»]
ProteinModelPortaliP29148.
SMRiP29148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi886882.PPSC2_c4320.

Protein family/group databases

MEROPSiM04.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105D4Y. Bacteria.
COG3227. LUCA.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPRE_PAEPO
AccessioniPrimary (citable) accession number: P29148
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.